In vitro germination and seedling development of tunisian caper (Capparis spinosa L.)

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Research Paper 01/01/2017
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In vitro germination and seedling development of tunisian caper (Capparis spinosa L.)

Rhimi Awatef, Hannachi Hédia, Hjaoujia Sonia, Yousfi Haifa, Boussaid Mohamed
Int. J. Agron. Agri. Res.10( 1), 1-8, January 2017.
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Abstract

Capparis spinosa L. (Capparaceae) is a perennial tropical and subtropical shrub plant known worldwide. Despite the increasing demand and economic importance of capers, little information is available regarding the propagation of this shrub. In fact, it is usually propagated by seed, but its percentage of germination is very low. In this report, in vitro seed germination and seedling development of four caper populations were studied and several treatments were evaluated to determine the ability of several factors to increase the percentage of germination. The seeds were treated with sulfuric acid (H2SO4) for 30 min. These seeds were left out in two different germination media (MS and H2O), for 30 days by soaking them in 1000 or 2000 mg.l-1 of GA3 each for 6, 12, 24 or 48 h. High variability was observed among the germination percentages of the different treatments varying from 4.16 to 75%. The variability was due to the Gibberellic acid level (GA3), to the genotype and to the media culture. The highest germination rate of 75% was obtained from the Nahli site seeds treated with 2000 mg.l-1 GA3 for 48 hours.
For all populations, regenerated plants were transplanted in pots on a sterile substrate. The rate of survival plants after acclimatization was 100%.

VIEWS 25

Andersson L, Milberg P. 1998. Variation in seed dormancy among mother plants, populations and year of seed collection. Seed Science Research 8, 29–38.

Arefi IH, Nejad SK, Kafi M. 2012. Roles of duration and concentration of priming agents on dormancy breaking and germination of caper (Capparis spinosa L.) for the protection of arid degraded areas. Pakistan Journal of Botany 44(1), 225-230.

Baskin CC, Baskin JM. 1998. Seeds: Ecology, biogeography, and evolution of dormancy and germination. Academic Press, San Diego, USA. xiv + 666 p.

Baytop T. 1999. Türkiye’de Bitkiler ile Tedavi (Geçmişte ve Bugün) – (Therapy with Medicinal Plants in Turkey – Past and Present). Nobel Tıp Kitabevi. 2. Baskı, Istanbul.

Bewley JD, Black M. 1994. Seeds: Physiology of Development and Germination. Plenum Press, New York.

Crunkilton DD, Garret HE, Pallardy SG. 1994. Growth and ectomycorgizal development of northern red oak seedlings treated with IBA. Scientia Horticulturae 29, 771–783

Eddouks M, Lemhadri A, Michel JB. 2005. Hypolipidemic activity of aqueous extract of Capparis spinosa L. in normal and diabetic rats. Journal of Ethnopharmacology 98, 345-350.

Kyauk H, Hopper NW, Brigham RD. 1995. Effect of temperature and pre-soaking on germination, root length and shoot length sesame (Sesamum indicum L.). Journal Environmental and Experimental Botany 35, 345–51

Murashige T, Skoog F. 1962. A revised medium for rapid growth and bio-assays with tabacco tissues cultures. Physiologia Plantarum Journal 15, 473–497.

Rhizopoulou S, Loannidi E, Alexandredes N, Argiroupoulos A. 2006. A study on functional and structural traits of the nocturnal flowers of Capparis spinosa L. Journal of Arid Environments 66, 635-647.

Saifi N, Ibijbijen J, Echchgadda G. 2013. In vitro propagation via seeds of Capparis ovata Desf.  African Journal of Biotechnology 21(12), 3235-3238.

Sakcali MS, Bahadir H, Ozturk M. 2008. Eco-physiology of Capparis spinosa L.: A plant suitable for combating desertification. Pakistan Journal of Botany 40, 1481-1486.

Shankarraja NS, Sulikeri GS. 1993. Presowing treatments of seeds to improve germination in cardamom (Elettaria cardamomum L. Maton var minor Watt). Journal of Plantation Crops 21, 116–127

Shirazi AM. 2003. Standardizing methods for evaluating the chilling requirements to break dormancy in seeds and buds (including geophytes): introduction to the workshop. Scientia Horticulturae 38, 334-335.

Soyler D, Arslan N. 1999. Effect of heat, light and dark treatments on seed germination of capers (Capparis spinosa L.). Anadolu 9, 63-75.

Soyler D, Khawar MK. 2007. Seed Germination of Caper (Capparis ovata var. Herbacea) Using α Naphthalene Acetic Acid and Gibberellic Acid. International Journal of Agriculture and Biology 9, 35–37

Sozzi G, Chiesa A. 1995. Improvement of caper (Capparis spnosa L.) seed germination by breaking seed coat-induced dormancy. Scientia Horticulturae 62, 255-261.

Swaminathaan C, Srinivasan M. 1996. Seedling investigation through plant growth substances in teak (Tectona grandis). Journal of Tropical Forest Science 8, 310–326

Zohary M. 1960. The species of Capparis in the Mediterranean and the Near Eastern Countries. Bulletin of the Research Council of Israel 8, 49–64.