International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Metabolic response of tomato (Lycopersicon esculentum Mill.) under salt stress combined with hormones

By: M. Arbaoui, M. Belkhodja

Key Words: Germination, Phytohormones, Processing tomato, Salinity, Soluble sugar and total proteins.

Int. J. Agron. Agri. Res. 12(1), 37-45, January 2018.

Certification: ijaar 2018 0069 [Generate Certificate]

Abstract

Effect of the combined the phytohormones-salinity (ABA, salicylic acid and GA3) on the total soluble protein and soluble sugars content contained in germinated seeds of two tomato varieties (Lycopersicon esculentum Mill.) is realized to characterize the soluble metabolite involvement in the processes of adaptation to salinity. The research of plant species tolerant to salinity requires an analysis of the behavior of the plant during its cycle especially during the phase of seed germination. At this stage of the seed, salinity is not the only constraining factor, the hormonal action is to be included. Thus, we propose an analysis of the seeds of two varieties of Rio Grande and Imperial tomatoes during their germination under salt stress at 100 mM NaCl.l-1 of distilled water, with or without added ABA at 0.005 mM, GA3 0.005 mM and SA at 0.5 mM. The observations are carried out in two stages at 48 and 96 hours of sowing show that the total soluble protein content and the soluble sugars are influenced by the different treatments; on the other hand, salicylic acid such as GA3 improves protein contents, salt stress and SA increase the soluble sugar content in germinated seeds. In contrast, the proteins decrease in seaweed associated with the ABA’s seeds of two varieties of processing tomato. On the other hand, a variability of the two quantified parameters of the germinated seed is expressed according to the treatment and between the two stages of germination.

| Views 85 |

Metabolic response of tomato (Lycopersicon esculentum Mill.) under salt stress combined with hormones

Afzal I, Shahzad MA, Basra MF, Nawaz A. 2006. Alleviation of Salinity Stress in Spring Wheat by Hormonal Priming with ABA, Salicylic Acid and Ascorbic Acid. International Journal of Agriculture Biology 1, 23-28.

Agamy RA, Hafez EE, Taha TH. 2013. Acquired Resistant Motivated by Salicylic Acid Applications on Salt Stressed Tomato (Lycopersicon esculentum Mill.). American-Eurasian Journal of Agricultural and Environmental Sciences 13, 50-57.

Amirjani MR. 2010. Effect of NaCl on Some Physiological Parameters of Rice. EJBS 3(1), 6-16.

Argyropoulou KE. 2011. Response of four greenhouse pepper hybrids to NaCl salinity- http://dspace.lib.cranfield.ac.uk/handle/1826/7140

Ashraf MY, Afaf R, Qureshi MS, Sarwar G, Naqvi MH. 2002. Salinity induced changes in α-amylase and protease activities and associated metabolism in cotton varieties during germination and early seedling growth stages. Acta Physiologiae Plantarum, 24(1), 37-44.

Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry 72(1-2), 248-254.

Bradford KJ, Downie AB, Gee OH, Alvarado V, Yang H, Dahal P. 2003. Abscisic acid and gibberellin differentially regulate expression of genes of the SNF1-related kinase complex in tomato seeds. Plant Physiol. 132, 1560-76.

Darwin C. 1856. On the Action of Sea‐water on the Germination of Seeds. Botanical Journal of the Linnean Society 1(3), 130-140.

Dong CJ, Wang XL, Shang QM. 2011. Salicylic acid regulates sugar metabolism that confers tolerance to salinity stress in cucumber seedlings.

Scientia Horticulturae, 129(4), 629-636 P.

DuBois M, Gilles KA, Hamilton JK, Rebers PT, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Analytical chemistry, 28(3), 350-356.

Eraslan F, Inal A, Gunes A, Alpaslan M, Atikmen NC. 2015. Comparative Physiological and Growth Responses of Tomato and pepper Plants to Fertilizer Induced Salinity and Salt Stress. Fresenius Environmental Bulletin, 24(5 A), 1774-1778.

Fait A, Angelovici R, Less H, Ohad I, Urbanczyk-Wochniak E, Fernie AR, Arabidopsis seed development and germination is associated with temporally distinct metabolic switches. Plant Physiol. 2006; 142, 839–54.

Hameed A, Afzal I, Iqbal N. 2010. Seed priming and salinity induced variations in wheat (Triticum aestivum L.) leaf protein profile. Seed Science and Technology, 38(1), 236-241.

Huang J, Redmann RE. 1995. Salt tolerance of Hordeum and Brassica species during germination and early seedling growth. Canadian Journal of Plant Science, 75(4), 815-819.

Iqbal M, Ashraf M. 2013. Gibberellic acid mediated induction of salt tolerance in wheat plants: growth, ionic partitioning, photosynthesis, yield and hormonal homeostasis. Environmental and Experimental Botany, 86, 76-85.

Jamil M, Lee KJ, Kim JM, Kim HS, Rha ES. 2007. Salinity reduced growth PS2 photochemistry and chlorophyll content in radish. Scientia Agricola, 64(2), 111-118.

Jat NK, Sharma V. 2006. The interactive effect of salinity and PGR on certain biochemical parameters in wheat seedlings. American Journal of Plant Physiology, 1(2), 132-141.

Kaur S, Gupta AK, Kaur N. 2002. Effect of osmo-and hydropriming of chickpea seeds on seedling growth and carbohydrate metabolism under water deficit stress. Plant growth regulation, 37(1), 17-22.

Lee S, Kim SG, Park CM. 2010. Salicylic acid promotes seed germination under high salinity by modulating antioxidant activity in Arabidopsis. New Phytologist, Vol. 188, Issue 2, 626-637 P.

MartínezAndújar C, Pluskota WE, Bassel GW, Asahina M, Pupel P, Nguyen TT, Fait A. 2012. Mechanisms of hormonal regulation of endosperm cap‐specific gene expression in tomato seeds. The plant journal, 71(4), 575-586.

Nawaz F, Ashraf MY, Ahmad R, Waraich EA. 2013. Selenium (Se) seed priming induced growth and biochemical changes in wheat under water deficit conditions. Biological trace element research 151(2), 284-293.

Nonogaki H, Bassel GW, Bewley JD. 2010. Germination-still a mystery. Plant Science, 179(6), 574-581.

Parvaneh R, Hosseini SM, Tavakoli S. 2012. The studying effect of drought stress on germination, proline, sugar, lipid, protein and chlorophyll content in purslane (Portulaca oleracea L.) leaves. Magnesium, 2(4), 7.

Price J, Laxmi A, Martin SKS, Jang JC. 2004. Global transcription profiling reveals multiple sugar signal transduction mechanisms in Arabidopsis. The Plant Cell, 16(8), 2128-2150.

Rajjou L, Duval M, Gallardo K, Catusse J, Bally J, Job C, Job D. 2012. Seed germination and vigor. Annual review of plant biology 63, 507-533.

Rosental L, Nonogaki H, Fait A. Activation and regulation of primary metabolism during seed germination. Seed Sci Res. 2014; 24, 1–15.

Shahba Z, Baghizadeh A, Yosefi M. 2010. The  salicylic acid effect on the tomato (Lycopersicum esculentum Mill.) germination, growth and photosynthetic pigment under salinity stress (NaCl). Journal of Stress Physiology and Biochemistry. 6(3), 4-16.

Shahba Z, Baghizadeh A, Yousefi M, Ohadi M. 2014. Effect of Salicylic Acid on Oxidative Stress Caused by NaCl Salinity in Lycopersicum esculentum Mill. Research Journal of Environmental Sciences, 8(1), 49.

Shi H, Wang X, Ye T, Chen F, Deng J, Yang P, Chan Z. 2014. The Cysteine2/Histidine2-Type transcription factor Zinc Finger of Arabidopsis thaliana 6 modulates biotic and abiotic stress responses by activating salicylic acid-related genes and C-Repeat-Binding Factor genes in Arabidopsis. Plant physiology 165(3), 1367-1379.

Wu D, Cai S, Chen M, Ye L, Chen Z, Zhang H, Zhang G. 2013. Tissue metabolic responses to salt stress in wild and cultivated barley. PLoS one, 8(1), e55431.

Zapata PJ, Serrano M, Pretel MT, Amorós A, Botella MÁ. 2004. Polyamines and ethylene changes during germination of different plant species under salinity. Plant Science, 167(4), 781-788.

M. Arbaoui, M. Belkhodja.
Metabolic response of tomato (Lycopersicon esculentum Mill.) under salt stress combined with hormones.
Int. J. Agron. Agri. Res. 12(1), 37-45, January 2018.
https://innspub.net/ijaar/metabolic-response-tomato-lycopersicon-esculentum-mill-salt-stress-combined-hormones/
Copyright © 2018
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update