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The agrA expression linked to quorum sensing-mediated coagulase formation using philippine ilongot ethnobotanicals against Staphylococcus aureus

By: Gabrielle T. Salamanca, Somar Israel D. Fernando, Khristina G. Judan Cruz

Key Words: Staphylococcus aureus, Quorum sensing, Coagulase, Ilongot, Ethnobotanicals, agrA.

Int. J. Biosci. 15(2), 33-41, August 2019.

DOI: http://dx.doi.org/10.12692/ijb/15.2.33-41

Certification: ijb 2019 0078 [Generate Certificate]

Abstract

Staphylococcal infections are among the most common hospital-acquired infections worldwide. Quorum sensing in bacterium is vital to their pathogenicity and the use of quorum sensing inhibition (QSI) compounds reveals opportunities to target virulence without developing resistance and suggests a strategic approach to develop drugs against pathogenic bacteria.  Ethnobotanical crude extracts (ECEs) of the Ilongot community of Aurora province, Philippines were tested for the QSI activity against coagulase formation in the reference strain Staphylococcus aureus. Antibacterial testing of extracts was done as pre-screening for coagulase formation.  Extracts were evaluated through tube coagulase assay for coagulase formation. Molecular confirmation of quorum sensing inhibition as affected by the ECEs was done through gene expression analysis of agrA. Antibacterial activity was observed in Urena lobata leaves, Dillenia philippinensis leaves and Diplazium esculentum. Coagulation was inhibited in tubes treated with crude extracts of Dillenia philippinensis bark, Stachytarpeta jamaicensis leaves, and Eleusine indica roots therefore, exhibiting QSI activities against S. aureus. S. jamaicensis leaves. E. indica roots and D. philippinensis bark significantly downregulated agrA therefore, confirming the QSI activity. The results indicate the potential of these ethnobotanicals for therapeutic approach in inhibiting bacterial virulence without developing resistance.

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The agrA expression linked to quorum sensing-mediated coagulase formation using philippine ilongot ethnobotanicals against Staphylococcus aureus

Adeloye A, Akinpelu D, Ogundaini OA, Obafemi C. 2007. Studies on Antimicrobial, Antioxidant and Phytochemical Analysis of Urena lobata Leave Extract. Journal of Physical and Natural Sciences 1, 1-9.

Adonizio AL, Downum K, Bennett BC, Mathee K. 2006. Anti-Quorum Sensing Activity of Medicinal Plants in Southern Florida. Journal of Ethnopharmacology 105(3), 427-435.

Akter S, Hossain MM, Ara I, Akhtar P. 2014. Investigation of In vitro Antioxidant, Antimicrobial and Cytotoxic Activity of Diplazium esculentum (Retz.) Sw. International Journal of advances in Pharmacy, Biology and Chemistry 3(3), 723-733.

Alaekwe IO, Ajiwe VI, Ajiwe A, Aningo G. 2015. Phytochemical and Anti–microbial screening of the Aerial Parts of Eleusine indica. International Journal of Pure & Applied Bioscience 3(1), 257-264.

Amit S, Sunil K, Sp B, Arvind N. 2011. Antibacterial activity of Diplazium esculentum (Retz.) Sw. Pharmacognosy Journal 3(21), 77-79.

Balberona AN, Noveno JJ, Angeles MGB, Santos RI, Cachin E. 2018. Ethnomedicinal Plants Utilized by the Ilongot-Eǵongot Community of Bayanihan, Maria Aurora, Aurora, Philippines. International Journal of Agricultural Technology 14(2), 145-159.

Barcelo RC. 2015. Phytochemical Screening and Antioxidant Activity of Edible Wild Fruits in Benguet, Cordillera Administrative Region, Philippines. Electronic Journal of Biology 11(3), 80-89.

Barrogo K, Cruz KJ, Jacinto WR. 2015. Quorum Sensing-Inhibition Activities of Philippine Ethnobotanicals against Virulence Factors in Pseudomonas aeruginosa. International Journal of Biosciences 13, 173-182.

Bassetti M, Trecarichi EM, Mesini A, Spanu T, Giacobbe DR, Rossi M, Shenone E, Pascale GD, Molinari MP, Cauda R, Viscoli C, Tumbarello M. 2011. Risk Factors and Mortality of Healthcare-Associated and Community-Acquired Staphylococcus aureus bacteraemia. Clininical Microbiology and Infection 18(9), 862-869.

Boucher H, Miller LG, Razonable RR. 2010. Serious Infections Caused by Methicillin-Resistant Staphylococcus aureus. Clinical Infectious Diseases 51, S183–S197.

Burdette SD, Watkins RR, Wong KK, Mathew SD, Martin DJ, Markert RJ. 2012. Staphylococcus aureus Pyomyositis Compared with Non-Staphylococcus aureus Pyomyositis. Journal of Infection 64(5), 507-512.

Camilli A, Bassler BL. 2006. Bacterial Small-Molecule Signaling Pathways. Science 311, 1113–1116.

Changchien CH, Chen YY, Chen SW, Chen WL, Tsay JG, Chu C. 2011. Retrospective Study of Necrotizing Fasciitis and Characterization of its Associated Methicillin-Resistant Staphylococcus aureus in Taiwan. BMC Infectious Diseases 11(297), 1-11.

Cheng AG, McAdow M, Kim HK, Bae T, Missiakas DM, Schneewind O. 2010. Contribution of Coagulases towards Staphylococcus aureus Disease and Protective Immunity. PLoS Pathogens 6(8), 19-20.

Cheung GY, Wang R, Khan BA, Sturdevant DE, Otto M. 2011. Role of the Accessory Gene Regulator agr in Community-Associated Methicillin-Resistant Staphylococcus aureus Pathogenesis. Infection and Immunity 79, 1927–1935.

Cruz KG, Gatchalan JJC, Jacinto WR. 2018. Philippine Ethnobotanicals Inhibit Quorum Sensing-Controlled Biofilm Formation in Pseudomonas aureginosa. International Journals of Biology, Pharmacy and Allied Sciences 7(4), 527-537.

Damle S, Sharon K. 2017. Phytochemical Studies of Ixora coccinea Linn – An Ethnobotanical Plant from Karwar district. International Journals of Biology, Pharmacy and Allied Sciences 6(7), 1403-1415.

DeLeo FR, Diep BA, Otto M. 2009. Host Defense and Pathogenesis in Staphylococcus aureus Infections. Infectious Disease Clinics of North America 23(1), 17–34.

Desai AV, Patil VM, Patil SS, Yeligar VC, Patil SV. 2017. Phytochemical Investigation of Eleusine indica for In-vivo Diuretic and In-vitro Anti-urolithiatic Activity. World Journal of Pharmaceutical Research 6(8), 1292-1304.

Dunman PM, Murphy E, Haney S, Palacios D, Tucker-Kellogg G, Wu S, Brown EL, Zagursky RJ, Shales D, Projan SJ. 2001. Transcription Profiling-Based Identification of Staphylococcus aureus Genes Regulated by the agr and/or sarA Loci. Journal of Bacteriology 183(24), 7341-7353.

Idu M, Omogbai EKI, Aghimien GE, Amaechina F, Timothy O, Omonigho SE. 2007. Preliminary Phytochemistry, Antimicrobial Properties and Acute Toxicity of Stachytarpheta jamaicensis (L.) Vahl. Leaves. Trends in Medical Research 2(4), 193-198.

Kalia VC, Rani A, Lal S, Cheema S, Raut CP. 2007. Combing Databases Reveals Potential Antibiotic Producers. Expert Opinion on Drug Discovery 2(2), 211-224.

Kechrid A, Pérez-Vázquez M, Smaoui H, Hariga D, Rodríguez-Baños M, Vindel A, Baquero F, Cantón R, Del Campo R. 2011. Molecular Analysis of Community-Acquired Methicillin-Susceptible and Resistant Staphylococcus aureus Isolates Recovered from Bacteraemic and Osteomyelitis Infections in Children from Tunisia. Clinical Microbiology and Infection 17(7), 1020-1026.

Koenig RL, Ray JL, Maleki SJ, Smeltzer MS, Hurlburt BK. 2004. Staphylococcus aureus AgrA Binding to the RNAIII-agr Regulatory Region. Journal of Bacteriology 186(22), 7549-7555.

Koh CL, Sam CK, Yin WF, Tan L, Krishnan T, Chong Y, Chan KG. 2013. Plant-Derived Natural Products as Sources of Anti-Quorum Sensing Compounds. Sensors 13(5), 6217–6228.

Kreienbuehl L, Charbonney E, Eggimann P. 2011. Community-Acquired Necrotizing Pneumonia Due to Methicillin-Sensitive Staphylococcus aureus Secreting Panton-Valentine Leukocidin: A Review of Case Reports. Annuals of Intensive Care 1, 1-7.

Limos GBB, Jacinto WR, Cruz KJ. 2015. Philippine Ethnobotanicals Inhibit Virulence Factors in Staphylococcus aureus. International Journal of Biosciences 13, 178-187.

McClure J, Conly JM, Lau G. 2006. Novel Multiplex PCR Assay for Detection of the Staphylococcal Virulence Marker Panton-Valentine Leukocidin Genes and Simultaneous Discrimination of Methicillin-Susceptible From Resistant Staphylococci. Journal of Clinical Microbiology 44, 1141-1144.

Mutungwa W, Alluri N, Majumdar M. (2015). Anti-Quorum Sensing Activity of Some Commonly Used Traditional Indian Spices. International Journal of Pharmacy and Pharmaceutical Sciences 7, 80-83.

Novick RP, Geisinger E. 2008. Quorum Sensing in Staphylococci. Annual Review of Genetics 42, 541-564.

Queck SY, Jameson-Lee M, Villaruz AE, Bach TL, Khan BA, Sturdevant DE, Ricklefs SM, Li M, Otto M. 2008. RNAIII-Independent Target Gene Control by the agr Quorum-Sensing System: Insight Into the Evolution of Virulence Regulation in Staphyloccocus aureus. Molecular cell 32(1), 150-158.

Ragasa C, Alimboyoguen AB, Shen C. 2009. Antimicrobial Triterpenes from Dillenia philippinensis. The Philippine Scientist 46(1), 78-87.

Rajkumari J, Borkotoky S, Murali A, Suchiang K, Mohanty SK, Busi S. 2018. Attenuation of Quorum Sensing Controlled Virulence Factors and Biofilm Formation in Pseudomonas aeruginosa by Pentacyclic Triterpenes, Betulin and Betulinic Acid. Microbial Pathogenesis 118, 48–60.

Rasmussen TB. Givskov M. 2006. Quorum-Sensing Inhibitors as Anti-Pathogenic Drugs. International Journal of Medicinal Microbiology 296(2-3), 149-61.

Rezai A, Oyong GG, Borja VB, Inoue M, Abe T, Tamamura R, Nagatsuka H, Setsu K, Buery RR. 2011. Molecular Screening of Anti-Quorum Sensing Capability of Salvadora persica on Enterococcus faecalis. Journal of Hard Tissue Biology 20(2), 115-124.

Sasidharan S, Latha L, Y, Zuraini Z, Suryani S, Sangetha S, Shirley L. 2007. Antidiarrheal and Antimicrobial Activities of Stachytarpheta jamaicensis Leaves. Indian Journal of Pharmacology 39(5), 245.

Smith RS, Iglewski BH. 2003. Pseudomonas aeruginosa Quorum-Sensing Systems and Virulence. Current Opinion in Microbiology 6(1), 56-60.

Song C, Ma H, Zhao Q, Song S, Jia Z. 2012. Inhibition of Quorum Sensing Activity by Ethanol Extract of Scutellaria baicalensis Georgi. Journal of Plant Pathology and Microbiology 72, 443.

Srisawat S. 2007. Effect of Some Thai Medicinal Plant Extracts on Antibacterial Activity of Periodontopathic Bacteria and Their Anti- Inflammatory Activity and Toxicity to Gingival Connective Tissue Fibroblast. Prince of Songkla University.

Sully EK, Malachowa N, Elmore BO, Alexander SM, Femling JK, Gray BM, DeLeo FR, Otto M, Cheung AL, Edwards BS, Sklar LA, Horswill AR, Hall PR, Gresham HD. 2014. Selective Chemical Inhibition of agr Quorum Sensing in Staphylococcus aureus Promotes Host Defense With Minimal Impact on Resistance. PLoS Pathogens 10(6), 1-14.

Tan LY, Yin WF, Chan KG. 2013. Piper nigrum, Piper betle and Gnetum gnemon-Natural Food Sources with Anti-Quorum Sensing Properties. Sensors 13(3), 3975-3985.

Thangiah A. 2019. Phytochemical Screening and Antimicrobial Evaluation of Ethanolic-Aqua Extract of Stachytarpheta jamaicensis (L.) vahl Leaves Against Some Selected Human Pathogenic Bacteria. Rasyan Journal of Chemistry 12(1), 300-307.

Tortora GJ, Funke BR, Case CL. 2013. Microbiology: An Introduction (11 ed.). Glenview, IL Pearson Education Inc.

Wielders CLC, Fluit AC, Brisse S, Verhoef J, Schmitz FJ. 2002. mecA Gene is Widely Disseminated in Staphylococcus aureus Population. Journal of Clinical Microbiology 40(11), 3970-3975.

Gabrielle T. Salamanca, Somar Israel D. Fernando, Khristina G. Judan Cruz.
The agrA expression linked to quorum sensing-mediated coagulase formation using philippine ilongot ethnobotanicals against Staphylococcus aureus.
Int. J. Biosci. 15(2), 33-41, August 2019.
https://innspub.net/ijb/agra-expression-linked-quorum-sensing-mediated-coagulase-formation-using-philippine-ilongot-ethnobotanicals-staphylococcus-aureus/
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