The alleviation of lead toxicity in Vicia faba L. by the application of selenium
By: Naima Layachi, Assia Amri, Zine Kechrid
Key Words: Lead, selenium, Vicia faba L., Antioxidant enzymes, Oxidative stress.
Int. J. Biosci. 12(5), 1-12, May 2018.
Certification: ijb 2018 0025 [Generate Certificate]
Selenium (Se) is an essential element for humans but is not considered as essential for plants. However, its beneficial role in improving plant growth and stress tolerances is well established. In order to study the role of Se in lead (Pb) toxicity in Faba bean (Vicia faba L.).This experiment was carried out on plants grown for 2 weeks on Hoagland medium supplied with 50 μM Pb in the form of lead nitrate Pb(NO3)2 and/or Se concentrations of 1.5 and 6 μM in the form of sodium selenite Na2SeO3 with three replications. The result showed that Pb decreased chlorophyll “a” and chlorophyll “b”. However, supplementation of Se restores the negative effect of Pb and increases pigment content. Osmolytes (soluble sugar and proline) were increased under Pb stress and further increase was observed with addition of Se. Pb also increased production of lipid peroxidation and the activities of antioxidant enzymes such as catalase and ascorbate peroxidase. Supplementation of Se decreased accumulation of lipid peroxidation and increased the activities of antioxidant enzymes to greater levels in roots and shoots.
The data suggest that Se might have an important protective effect in plants under Pb and may help to alleviate the adverse effect of Pb on the growth of Vicia faba L.
The alleviation of lead toxicity in Vicia faba L. by the application of selenium
Ahmad R, Waraich EA, Nawaz F, Ashraf MY, Khalid M. 2016. Selenium (Se) improves drought tolerance in crop plants–amythorfact? J Sci Food Agric 96, 372–380.
Ardebili NO, Saadatmand S, Niknam V, Khavari-Nejad RA. 2014.The alleviating effects of selenium and salicylic acid in salinity exposed soybean. Acta Physiol. Plant 36, 3199–3205.
Bañuelos GS, Fakra SC, Walse SS, Marcus MA, Yang SI, Pickering IJ. 2011. Selenium accumulation ,distribution, and speciation in spineless prickly pear cactus:a drought-and salt-tolerant, selenium en richednutraceutical fruit crop forbiofortified foods. Plant Physiol 155, 315–327.
Broadley MR, Alcock J, Alford J, Cartwright P, Foot I, Fairweather-Tait SJ. 2010. Selenium bio fortification of high-yielding winter wheat (Triticum aestivum L.) by liquid orgranular Se fertilisation. Plant Soil 332, 5–18.
Cartes P, Gianfera L, Mora ML. 2005. Uptake of selenium and its antioxidative activity in ryegrass when applied as selenate and selenite forms. Plant Soil 276, 359–367.
Chatterjee C, Dube BK, Sinha P, Srivastava P. 2004. Detrimental effects of Pbphytotoxicity on growth, yield and metabolism of rice. Comm Soil Sci Plant An 35(1-2), 255-265.
Djanaguiraman M, Prasad PVV, Seppänen M. 2010. Selenium protects sorghum leaves from oxidative damage under high temperature stress by enhanc-ing antioxidant defense system. Plant Physiology and Biochemistry 48(12), 999–1007.
Ellis DR, Salt DE. 2003. Plants,selenium and human health. Curr Opin Plant Biol 6, 273–279.
Feng R, Wei C, Tu S. 2013. The roles of selenium in protecting plants against abiotic stresses. Environ Exp Bot. 87, 58–68.
Filek M, Keskinen R, Hartikainen H, Szarejko I, Janiak A, Miszalski Z. 2008. The protective role of selenium in rape seedlings subjected to cadmium stress. J Plant Physiol 165, 833–844.
Gopal R, Rizvi AH. 2008. Excess Pb alters growth, metabolism and translocation of certain nutrients in radish. Chemosphere 70(9), 1539-1544.
Han D, Xiong S L, Tu SX, Liu JC, Chen C. 2015.Interactive effects of selenium and arsenicon growth, antioxidant system, arsenic and selenium species of NicotianatabacumL. Environ ExpBot 117, 12–19.
Hartikainen H, Xue T, Piironen V. 2000 .Selenium as an anti-oxidant and pro-oxidant in ryegrass. Plant Soil 225, 193–200.
Hartikainen H, Xue T, Piironen V. 2000. Selenium asan anti-oxidant and pro-oxidant in ryegrass. Plant Soil 225, 193–200.
Hartikainen H. 2005. Biogeochemistry of selenium and it sim pact on food chain quality and human health. J Trace Elem Med Biol 18, 309–318.
Hasanuzzaman M, Hossain MA, Fujita M. 2011. Selenium-induced up-regulation of the antioxidant defense and methylglyoxal detoxification system reduces salinity-induced damage in rapeseed seedlings. Biological Trace Element Research 143, 1704–1721.
Hatfield DL, Tsuji PA, Carlson BA, Gladyshev VN. 2014. Selenium and selenocysteine:roles in cancer ,health,and development. Trends Biochem Sci 39, 112–120.
Hawrylak-Nowak B, Dresler S, Wo´jcik M. 2014. Selenium affects physiological parameters and phytochelatins accumulation in cucumber (Cucumis sativus L.) plants grown under cadmium exposure.SciHortic172, 10–18.
Hawrylak-Nowak B. 2009. Beneficial effects of exogenous selenium in cucumber seedlings subjected to salt stress. Biol Trace Elem Res 132,259–269.
Heath RL, Packer L. 1968.Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125, 189–198.
Hoagland DR, Arnon DJ. 1959. The water-culture method of growing plants without soil. Calif Agric ExpStn Circ 347, 26–29.
Holden M.1975. Chlorophylls in chemistry and biochemistry of plant pigment. 2nd Ed. Academie Press, New York, 133.
Kumar M, Bijo AJ, Baghel RS, Reddy CRK, Jha B. 2012. Selenium and spermine alleviates cadmium induced toxicity in the red seaweed Gracilaria dura by regulating antioxidant system and DNA methylation. Plant PhysiolBiochem 51, 129–138.
Lee DH, Kim YS, Lee C B. 2001.The inductive response of the antioxidant enzymes by salt stress in the rice (Oryza sativa L.). Plant Physiol 158, 737–745.
Lin L, Zhou WH, Dai HX, Cao FB, Zhang G, P, Wu FB. 2012. Selenium reduces cadmium uptake and mitigates cadmium toxicity in rice. J Hazard Mater 235, 343–351.
Liu DT, Li X, Jin X, Yang E, Islam Q. 2008. Pb induced changes in the growth and antioxidant metabolism of the Pb accumulating and non-accumulating ecotypes of Sedum alfredii. J Integr Plant Biol 50(2), 129-140.
Loggini B, Scartazza A, Brugnoli E, Navari- Izzo F. 1999. Antioxidative defense system, pigment composition and photosynthetic efficiency in two wheat cultivars subjected to drought. Plant Physiology 119, 1091-1099.
Majumder A, Sengupta S, Goswami L. 2010. Osmolyte regulation in abiotic stress. In: Pareek A, Sopory SK, Bohnert HJ, Ed. Abiotic stress adaptation in plants, Springer, Netherlands, 349–370.
Malik JA, Goel S, Kaur N, Sharma S, Singh I, Nayyar H. 2012. Selenium antagonizes the toxic effects of arsenic on mungbean (Phaseolus aureus Roxb.) plants by restricting its uptake and enhancing the antioxidative and detoxification mechanisms. Environ Exp.Bot77, 242–248.
Malik JA, Goel S, Kaur N, Sharma S, Singh I, Nayyar H. 2012. Selenium antag-onises the toxic effects of arsenic on mungbean (Phaseolus aureus Roxb.) plants by restricting its uptake and enhancing the antioxidative and detoxification mechanisms. Environmental and Experimental Botany 77, 242–248.
Mittler R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7(9), 405–410.
Monneveux PH, Nemmar M. 1986. Contribution
à l’étude de la résistance à la sècheresse chez le blé tendre. Etude de l’accumulation de la proline au cours du cycle de développement. Agronomie.17.
Mozafariyan M, Shekari L, Hawrylak-Nowak B, Kamelmanesh MM. 2014. Protective role of selenium on pepper exposed to cadmium stress during reproductive stage. Biol Trace Elem. Res 160, 97–107.
Mroczek-Zdyrska M, Wojcik M. 2012 .The influence of selenium on root growth and oxidative stress induced by lead in Vicia faba L. minor plants. Biol Trace Elem. Res.147, 320–328.
Murkowski A, Skórska E. 2008. Comparison of phytotoxicity of lead and tin organic compounds by means of luminescence methods. Acta Agrophysica 11(1), 131-140.
Nakano Y, Asada K. 1981. Hydrogen Peroxide is scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts. Plant Cell and Physiology 22, 867-880. http://dx.doi.org/10.1093/oxfordjournals.pcp.a076232
Nawaz F, Ashraf MY, Ahmad R, Waraich EA. 2013. Selenium (Se) seed priming induced growth and biochemical changes in wheat under water deficit conditions. Biol Trace Elem Res 151,284–293.
Pennanen A, Tailin XUE, Hartikainen H. 2002. Protective role of selenium in plant subjected to severe UV irradiation stress. J Appl Bot 76, 66–76.
Pezzarossa B, Remorini D, Gentile ML, Massai R. 2012. Effects of foliar and fruit addition of sodium selenite on selenium accumulation and fruit quality. J Sci Food Agric 92, 781–786.
Pilon-Smits EAH, Quinn CF, Tapken W, Malagoli M, Schiavon M. 2009. Physiological functions of beneficial elements. Curr Opin Plant Biol 12, 267–274.
Pukacka S, Ratajczak E, Kalembam E. 2011. The protective role of selenium in recalcitrant Acer saccharium L. seeds subjected to desiccation. J Plant Physiol 168, 220–225.
Qing X, Zhao X, Hu C, Wang P, Zhang Y, Zhang X. 2015.Selenium alleviates chromium toxicity by preventing oxidative stress in cabbage(Brassica campestris L. ssp. Pekinensis) leaves. Ecotoxicol Environ Saf 114, 179–189.
Rayman MP. 2000. The importance of selenium to human health. Lancet 356, 233–241.
SaidiI, Chtourou Y, Djebali W. 2014 .Selenium alleviates cadmium toxicity by preventing oxidative stress insunflower(Helianthus annuus) seedlings. J Plant Physiol 171, 85–91.
Schields R, Burnett W. 1960. Determination of protein bound carbohydrate in serum by a modified anthrone method. Anal Chem 32, 885-886
Shanker K, Mishra S, Srivastava S, Srivastava R, Daas S, Prakash S. 1996a. Effect of selenite and selenite on plant uptake and translocation of mercury by tomato(Lycopersicum esculentum). Plant Soil 183, 233–238.
Shanker K, Mishra S, Srivastava S, Srivastava R, Daas S, Prakash S. 1996b.Effect of selenite and selenite on plant uptake of cadmium by maize (Zea mays). Bull Environ Contam Toxicol 56, 419-
Sharma A, Bhushan JHA, Dubey RA. 2011. Oxidative stress and antioxidative defense systems in plants growing under abiotic stresses. In: Pessarakli M, Ed. Handbook of Plant and Crop Stress, CRC press, 89–138.
Sharma P, Dubey RS. 2005. Lead toxicity in plants. Brazilian Journal of Plant Physiology 17, 35-52.
Srivastava S, Shanker K, Srivastava S, Srivastav R, Das S, Prakash S. 1998.Effect of selenium supplementation on the uptake and translocation of chromium by spinach (Spinacea oleracea). Bull Environ Contam Toxicol 60, 750–758.
Terry N, Zayed AM, DeSouza MP, Tarun AS. 2000. Selenium in higher plants. Annu Rev Plant Physiol Plant Mol Biol. 51, 401–432.
Trumble JT, Kundm GS, White KK. 1998. Influence of form and quantity of selenium on the development and survival of an insect herbivore. Environ. Pollut. 101, 175–182.
Turakainen M, Hartikainen H, Seppanen MM. 2004. Effects of selenium treatments on potato (Solanum tuberosum L.) growth and concentrations of soluble sugars and starch. J Agric. Food Chem 52, 5378–5382.
Wang YD, Wang X, Wong YS. 2012. Proteomics analysis reveals multiple regulatory mechanisms in response to selenium in rice. Journal of Proteomics 75, 184-186.
Wierzbicka M, Potocka A. 2002. Lead tolerance in plants growing on dry and moist soil. Acta Biol Cracov Bot 44, 21–28.
Wu ZL, Yin XB, Bañuelos GS, Lin ZQ, Zhu Z, Liu Y. 2016 .Effect of selenium on control of postharvest gray mold of tomato fruit and the possible mechanisms involved. Front Microbiol 6, 1441.
Wu ZL, Bañuelos GS, Lin ZQ, Liu Y, Yuan LX, Yin XB. 2015. Bio fortification and phytoremediation of selenium in China. Front Plant Sci. 6, 136.
Yao X, Chu J, He X, Ba C. 2011. Protective role of selenium in wheat seedlings subjected to enhanced UV-B radiation. Russian Journal of Plant Physiology 58(2), 283–289.
Yao XQ, Chu JZ, Ba CJ. 2010a. Antioxidant responses of wheat seedlings to exoge-nous selenium supply under enhanced ultraviolet-B. Biological Trace Element Research 136(1), 96–105.
Yao XQ, Chu JZ, Wang GY. 2009a. Effects of selenium on wheat seedlings under drought stress. Biological Trace Element Research 130(3), 283–290.
Yao XQ, Chu JZ, Wang GY. 2009b. Effects of drought stress and selenium supply on growth and physiological characteristics of wheat seedlings. Acta Physiologies Plant arum 31(5), 1031–1036.
Yao, XQ, Chu JZ, Ba CJ. 2010b. Responses of wheat roots to exogenous selenium supply under enhanced ultraviolet-B. Biological Trace Element Research 137 (2), 244–252.
Yathavakilla SKV, Caruso JA. 2007 .A study of Se–Hg antagonism in Glycine max (soybean) roots by size exclusion and reversed phase HPLC–ICPMS. Anal Bioanal Chem 389, 715–723.
Zhu YG, Pilon-Smits EAH, Zhao FJ, Williams PN, Meharg AA. 2009. Selenium in higher plants: under standing mechanisms for bio fortification and phytoremediation. Trends Plant Sci 14, 436–442.
The alleviation of lead toxicity in Vicia faba L. by the application of selenium.
Int. J. Biosci. 12(5), 1-12, May 2018.
By Authors and International Network for
Natural Sciences (INNSPUB)