International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Antibacterial and antibiofilm activity of the phenazine extract of a fluorescent Pseudomonas on coagulase-negative staphylococci isolated from the Anti-Cancer Center of Batna, Algeria

By: Zatout Asma, Djibaoui Rachid, Dahah Hicham, Mazari Hibat Errahmen, Benkredda Fatima, Kassah-Laouar Ahmed

Key Words: Coagulase-negative staphylococci, Pseudomonas, Inhibition, Biofilm, Phenazine compounds

Int. J. Biosci. 15(3), 412-424, September 2019.

DOI: http://dx.doi.org/10.12692/ijb/15.3.412-424

Certification: ijb 2019 0250 [Generate Certificate]

Abstract

Faced with many failures in the elimination of CoNS pathogens, we aimed to find an effective agent to eradicate these bacteria. In the present study we tested 20 isolates of CoNS collected from the Anti-Cancer Center of Batna (Algeria). As antistaphylococcal agents we used in our experiment 29 Pseudomonas isolates selected from the roots of plant (Hordeum murinum). The results of crossed streak method on MHA medium showed that the 29 isolates of Pseudomonas inhibited 19 CoNS. The strain with the greater inhibitory effect PK inhibited 14 CoNS. The high performing Pseudomonas PK has been used for the production of phenazine compounds. The dry compound of PK from ethyl acetate extraction has been shown to be effective against 13 isolates of staphylococci. The percentage of biofilm inhibition by the PK phenazine extract was between (32.2%) and (77.56%). The analysis of the compound obtained by UV-visible and infrared spectrum showed that it was similar to hydroxyphenazine.

| Views 44 |

| Views 44 |

Antibacterial and antibiofilm activity of the phenazine extract of a fluorescent Pseudomonas on coagulase-negative staphylococci isolated from the Anti-Cancer Center of Batna, Algeria

Abalain-Collocml, Béraud J, Lay-Roguès GL, Tandé D, Tran Minoui A. 2014. Bacttériologie. In: Béraud J, Ed. Le technicien d’analyses biomédicales. 2ème édition, 936-1067.

Bauer AW, Kirby WMM, Sherris JC, Turck M. 1966. Antibiotic Susceptibility Testing by a Standardized Single Disk Method. American Journal of Clinical Pathology 45, 493-496.

Bazarganimm and Rohloff J. 2016. Antibiofilm activity of essential oils and plant extracts against Staphylococcus aureus and Escherichia coli biofilms. Food Control 61, 156-164.

Bertrand X, Lallemand S, Thouverez M, Boisson K, Talon D. 2002. Bactériémies liées aux staphylocoques à coagulase négative : incidence, niveau de résistance à la teicoplanine et épidémiologie moléculaire. Pathologie Biologie 50, 552-559.

Bonsall RF, Weller DM, Thomashow LS. 1997. Quantification of 2,4diacetylphloroglucinol produced by fluorescent Pseudomonas spp. in vitro and in the rhizosphere of wheat. Applied and Environmental Microbiology 3, 951-955.

Borrero NV, Bai F, Perez C, Duong BQ, Rocca JR, Jin S, Huigens III RW. 2014. Phenazine Antibiotic Inspired Discovery of Potent Bromophenazine Antibacterial Agents against Staphylococcus aureus and Staphylococcus epidermidis. Organic & Biomolecular Chemistry 1-6.

Bossis E, Lemanceau P, Latour X, Gardan L. 2000. The taxonomy of Pseudomonas fluorescens, Pseudomonas putida: current status and need for revision. Agronomie 20, 51-63.

Cardozo VF, Oliveira AG, Nishio EK, Perugini MRE, Andrade CGTJ, Silveira WD, Durán N, Andrade G, Kobayashi RKT, Nakazato G. 2013. Antibacterial activity of extracellular compounds produced by a Pseudomonas strain against methicillinresistant Staphylococcus aureus (MRSA) strains. Annals of Clinical Microbiology and Antimicrobials 12, 1-8.

Chain EB, Mellows G. 1977. Pseudomonic acid. Part 1: The structure of Pseudomonic acid A, a novel antibiotic produced by Pseudomonas fluorescens. Journal of the Chemical Society, Perkin Transactions 1, 294.

Christensen GD, Simpson WA, Yonger JJ, Baddor LM, Barrett FF, Melton DM, Beachey EH. 1985. Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. Journal of Clinical Microbiology 22, 996-1006.

Clinical and Laboratory Standard Institute. 2014. Performance standards for Antimicrobial Susceptibility; Twenty-Fourth informational supplement.

Cuevas O, Cercenado E, Vindel A, Guinea J, Sa´nchez-Conde M, Sa´nchez-Somolinos M, Bouza E, and the Spanish Group for the Study of Staphylococcus. 2004. Evolution of the Antimicrobial Resistance of Staphylococcus spp. In Spain: Five Nationwide Prevalence Studies, 1986 to 2002. Antimicrobial Agents and Chemotherapy 4240-4245.

Dahah H, Djibaoui R, Nemmiche S. 2016. Antimicrobial, antioxidant and hemolytic effects of Pyocyanin produced by Pseudomonas aeruginosa isolated from saline soil of Mina river, Algeria. International Journal of Biosciences 9, 134-143.

Delaney SM, Mavrodi DV, Bonsall RF, Thomashow LS. 2001. phzO, a gene for biosynthesis of 2-hydrolyated phenazine compounds in Pseudomonas aureofaciens 30–84. Journal of Bacteriology 183, 318-327.

Ehlersson G. Hellmark B, Svartström O, Stenmark B, Söderquist B. 2017. Phenotypic characterisation of coagulase-negative staphylococci isolated from blood cultures in newborn infants, with a special focus on Staphylococcus capitis. Acta paediatrica 106, 1576-1582.

El Amraoui B, El Amraoui M, Cohen N et al. 2014. Antifungal and antibacterial activity of marine microorganisms Annales Pharmaceutiques Françaises 72, 107-111.

Fauchère JL, Avril JL. 2005. Bactériologie générale et médicale. Ed. Ellipses 213-218.

Garnier F, Denis F. 2007. Cocci à Gram positif. In: Denis F., Ploy M. C., Martin C., Bingen E et Quentin R, Bactériologie médicale (Techniques usuelles). Ed. Elsevier Masson p. 252-257.

Grahl N, Kern SE, Newman DK, Hogan DA. 2013. The Yin and Yang of Phenazine Physiology. In: Chincholkar S, Thomashow L, Ed. Microbial Phenazines Biosynthesis, Agriculture and Health, Springer p. 48.

Haas D, Keel C. 2003. Regulation of antibiotic production in root-colonizing Pseudomonas spp. and relevance for biological control of plant disease. Annual Review of Phytopathology 41, 117-153.

Hall-Stoodley L, Costerton JW, Stoodley P. 2004. Bacterial biofilms: from the natural environment to infectious diseases. Nature Reviews Microbiology 2, 95-108.

Hazalin NA, Ramasamy K, Meng Lim SS, Abdul Wahab I, Cole ALJ, Abdul Majeed AB. 2009. Cytotoxic and antibacterial activities of endophytic fungi isolated from plants at the National Park, Pahang, Malaysia. BMC Complementary and Alternative Medicine 09, 1-5.

Herard A, Brasme L, Jaussaud R, Colin J, Vernet-Garnier V, Lardennois B. 1998. Place actuelle des staphylocoques à coagulase négative en urologie. Progrès en Urologie 8, 579-585.

Hotterbeekx A, Kumar-Singh S, Goossens H, alhotra-Kumar S. 2017. In vivo and In vitro Interactions between Pseudomonas aeruginosa and Staphylococcus spp. Frontiers in Cellular and Infection Microbiology 7, 1-13

Igbinosa IH, Igbinosa EO, Okoh AI. 2014. Molecular Detection of Metallo-β- Lactamase and Putative Virulence Genes in Environmental Isolates of Pseudomonas Species. Polish Journal of Environmental Studies. 23, 2327-2331.

Jacques P, Delfosse P, Ongena M, Lepoivre P, Cornélis P, Koedam N, Neirinckx L, Thonart P. 1993. Les mécanismes biochimiques développés par les Pseudomonas fluorescents dans la lutte biologique contre les maladies des plantes transmises par le sol. Cahiers Agricultures 2, 301-307.

Jain A, Agarwal J, Bansal S. 2004. Prevalence of methicillin-resistant, coagulase negative staphylococci in neonatal intensive care units: findings from a tertiary care hospital in India. Journal of Medical Microbiology 53, 941-944

Koksal F, Yasar H, Samasti M. 2009. Antibiotic resistance patterns of coagulase negative Staphylococcus strains isolated from blood cultures of septicemic patients in Turkey. Microbiological Research 164, 404-410.

Laine MH, Karwoski MT, Raaska LB, Sandholm TM. 1996. Antimicrobial activity of Pseudomonas spp. against food poisoning bacteria and moulds. Letters in Applied Microbiology 22, 214-218.

Laursen JB, Nielsen J. 2004. Phenazine Natural Products: Biosynthesis, Synthetic Analogues, and Biological Activity. Chemical Reviews 104, 1663-1685.

Leclercq R. 2002. Résistance des staphylocoques aux antibiotiques Annales francaises d’anesthesie et de réanimation 21, 375-383.

Leisinger T, Margraff R. 1979. Secondary Metabolites of the Fluorescent Pseudomonads. Microbiological Reviews 43, 422-442.

Mack D, Horstkotte MA, Rohde H, Knobloch JK-M. 2006. Coagulase-Negative Staphylococci. In: Pace JL, Rupp ME, Finch RG, Ed. Biofilms, Infection, and Antimicrobial Therapy. Taylor and Francis p.115.

Marino A, Bellinghieri V, Nostro A, Miceli N, Taviano MF, Güvenç A, Bisignano G. 2010. In vitro effect of branch extracts of Juniperus species from Turkey on Staphylococcus aureus biofilm. FEMS Immunology & Medical Microbiology 470-476.

Mashburn LM, Jett AM, Akins DR, Whiteley M. 2005. Staphylococcus aureus serves as an iron source for Pseudomonas aeruginosa during in vivo co-culture. Journal of Bacteriology 187, 554-566.

Mezaache-Aichour S, Guechi A, Nicklin J, Drider D, Prevost H, Strange RN. 2012. Isolation, identification and antimicrobial activity of pseudomonads isolated from the rhizosphere of potatoes growing in Algeria. Journal of Plant Pathology 94, 89-98.

Moloneymg. 2016. Natural products as a source for novel antibiotics. Trends in pharmacological sciences 37, 689-701.

Nansathit A, Apipattarakul S, Phaosiri C, Pongdontri P, Chanthai S, Ruangviriyachai C. 2009. Synthesis, Isolation of Phenazine Derivatives and Their Antimicrobial Activities. Walailak Journal of Science and Technology 6, 79-91.

Nasr RA, AbuShady HM, Hussein HS. 2012. Biofilm formation and presence of icaAD gene in clinical isolates of staphylococci. The Egyptian Journal of Medical Human Genetics 13, 269-274.

Nauciel C, Vildé JL. 2005. Bactériologie médicale connaissances et pratique. 2ème édition Masson Paris p. 53- 80.

Nostro A, Guerrini A, Marino A, Tacchini M, Di Giulio M, Grandini A, Akin M, Cellini L, Bisignano G, Saracoglu HT. 2016. In vitro activity of plant extracts against biofilm-producing food-related bacteria. International Journal of Food Microbiology 6, 79-91.

Pinna A, Zanetti S, Sotgiu M, Sechi LA, Fadda G, Carta F. 1999. Identification and antibiotic susceptibility of coagulase negative staphylococci isolated in corneal/external infections. British Journal of Ophthalmology 83, 771-773.

Qin Z, Yang L, Qu D, Molin S, Tolker-Nielsen T. 2009. Pseudomonas aeruginosa extracellular products inhibit staphylococcal growth, and disrupt established biofilms produced by Staphylococcus epidermidis. Microbiology 155, 2148-2156.

Qin Z, Yang X, Yang L, Jiang J, Ou Y, Molin S, et al. 2007. Formation and properties of in vitro biofilms of ica-negative Staphylococcus epidermidis clinical isolates. Journal of Medical Microbiology 56, 83-93.

Raut JS, Karuppayil SM. 2014. A status review on the medicinal properties of essential oils. Industrial Crops and Products 62, 250-264.

Rodrigues LB, Santos LRD, Tagliari VZ, Rizzo NN, Trenhago G, de Oliveira AP, Goetz F, do Nascimento VP. 2010. Quantification of biofilm production on polystyrene by Listeria, Escherichia coli and Staphylococcus aureus isolated from a poultry slaughterhouse. Brazilian Journal of Microbiology 41, 1082-1085.

Saleem M, Nazir M, Ali MS, Hussain H, Lee YS, Riaz N, Jabbar A. 2010. Antimicrobial natural products: an update on future antibiotic drug candidates. Natural Product Reports 27, 238-254.

Selvin J, Shanmughapriya S, Gandhimathi R, Seghal-Kiran G, Rajeetha RT, Natarajaseenivasan K, Hema TA. 2009. Optimization and production of novel antimicrobial agents from sponge associated marine actinomycetes Nocardiopsis dassonvillei MAD08. Applied Microbiology and Biotechnology 83, 435-445.

Shahid I, Rizwan M, Baig DN, Saleem RS, Malik KA, Mehnaz S. 2017. Secondary Metabolites Production and Plant Growth Promotion by Pseudomonas chlororaphis and P. aurantiaca Strains Isolated from Cactus, Cotton, and Para Grass. Journal of Microbiology and Biotechnology 27, 480-491.

Shrestha LB, Bhattarai NR, Khanal B. 2017. Antibiotic resistance and biofilm formation among coagulase-negative staphylococci isolated from clinical samples at a tertiary care hospital of eastern Nepal. Antimicrobial Resistance and Infection Control 6, 89.

Singh RP, Kunari P, Reddy CR. 2015. Antimicrobial compounds from sea-weeds associated bacteria and fungi. Applied Microbiology and Biotechnology 99, 1571-1586.

Soumya KR, Philip S, Sugathan S, Mathew J, Radhakrishnan EK. 2017. Virulence factors associated with Coagulase Negative Staphylococci isolated from human infections. 3 Biotech 7, 1-10.

Stepanovic S, Vukovi D, Hola V, Bonaventura G, Djukic S, C´irkovic´ I, Ruzicka F. 2007. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by Staphylococci. Journal of Pathology, Microbiology and Immunology 115, 891-899.

Veerendrakumar M, Janakiram P. 2015. Antagonistic Activity Exhibited by Crude Extracts of Pseudomonas aeruginosa (PIC-4) against Aeromona shydrophila and E. coli. International Journal of Life Sciences Biotechnology and Pharma Research 4, 36-41.

Veselova A, Klein SH, Bass IA, Lipasova VA, Metlitskaya AZ, Ovadis MI, Chernin LS, Khmel IA. 2008. Quorum sensing systems of regulation, synthesis of phenazine antibiotics, and antifungal activity in rhizospheric bacterium Pseudomonas chlororaphis 449. Russian Journal of Genetics 44, 1400-1408.

Zatout Asma, Djibaoui Rachid, Dahah Hicham, Mazari Hibat Errahmen, Benkredda Fatima, Kassah-Laouar Ahmed.
Antibacterial and antibiofilm activity of the phenazine extract of a fluorescent Pseudomonas on coagulase-negative staphylococci isolated from the Anti-Cancer Center of Batna, Algeria.
Int. J. Biosci. 15(3), 412-424, September 2019.
https://innspub.net/ijb/antibacterial-antibiofilm-activity-phenazine-extract-fluorescent-pseudomonas-coagulase-negative-staphylococci-isolated-anti-cancer-center-batna-algeria/
Copyright © 2019
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update