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Antibacterial potential of methanolic extract of Syzygiumaromaticum (clove) against different bacterial strains

By: Emad Mohamed Abdallah

Key Words: Antibacterial, cup-plat diffusion, cloves, Syzygiumaromaticum, Gram-positive, Gram-negative.

Int. J. Biosci. 16(2), 394-401, February 2020.

DOI: http://dx.doi.org/10.12692/ijb/16.2.394-401

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Abstract

The flower buds of Syzygium aromaticum (Cloves) are a well-known spice, prescriped for treatment from some microbial diseases since ancient civilizations and in traditional medicine today. In the currenrt investigation, the methanolic extract of cloves was tested against 4 Gram-positive bacteria , namely Bacillus cereus ATCC10876, Staphylococcus saprophyticus ATCC 43867, Enterococcus faecalis ATCC 29212 and Staphylococcus epidermidis ATCC 12228. In addition to 5 strains  of Gram-negative bacteria , namely  Proteus vulgaris ATCC 6380, Klebsiella pneumoniae ATCC 27736, Salmonella typhimurium ATCC 14028, Shigella flexneri ATCC 12022 and  Escherichia coli ATCC 25922, using two diffusion methods (Cup-plate and disc diffusion methods), besides MIC and MBC testings. The extract showed noticable dose-dependant antibacterial activity against all tested bacterial strains with varied degrees, and there was no significant differences between the results of the cup-plate diffusion assay and the disc-diffusion assay. Moreover, MIC values were between 3.9 to 125 mg/ml, and MBC values ranged between 7.8 to 125 mg/ml, which was higher than the MIC’s, the MBC/MIC ratio indicating that the extract has a bactericidal attitude, which makes it suitable source for the formulation of new antibacterial drugs.

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Antibacterial potential of methanolic extract of Syzygiumaromaticum (clove) against different bacterial strains

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Emad Mohamed Abdallah.
Antibacterial potential of methanolic extract of Syzygiumaromaticum (clove) against different bacterial strains.
Int. J. Biosci. 16(2), 394-401, February 2020.
https://innspub.net/ijb/antibacterial-potential-methanolic-extract-syzygiumaromaticum-clove-different-bacterial-strains/
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