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Antibacterial resistance pattern in isolates from pus samples: An observational study

By: Saifullah Khan Jadoon, Kainat Saeed, Anwar Khalid, Naveed Muhammad, Palwasha Khan, Faheem Jan

Key Words: Antibiotics, Antimicrobial resistance, observational study, Pus samples.

Int. J. Biosci. 15(4), 367-371, October 2019.

DOI: http://dx.doi.org/10.12692/ijb/15.4.367-371

Certification: ijb 2019 0323 [Generate Certificate]

Abstract

Development of antibacterial drug resistance has made the cure of bacterial wound infections very challenging, especially in post-operative cases. Current study was carried out at Ayub Teaching Hospital, Abbottabad. The aim of this study was to identify organisms developing resistance and to detect the predominant bacterial sketch and its antibiogram in Abbottabad. An observational study was directed for a period of 1 year from June 2016 to May 2017 at Ayub Teaching Hospital, Abbottabad. Pus samples from several locations were collected aseptically from 300 patients and were subjected to isolation and identification of aerobic bacteria by standard procedure. Out of the 300 clinical samples, 238 showed bacterial growth. Staphylococcus aureus was the most common organism isolated (57.5%), followed by Escherichia coli (19.72%), Klebsiella (12.28%), Pseudomonas (10.50%) among others. S. aureus was found to be highly resistant to penicillin, ampicillin, amoxicillin and nalidixic acid, while being sensitive to sipraxin and vancomycin. On the other hand, of the gram-negative bacilli isolated, E. coli was found to be more common, followed by Klebsiella, Pseudomonas, Proteus, and Acinetobacter. They were all found to be highly resistant to ciprofloxacin and amoxicillin and fairly sensitive to amikacin and azaetronam. This study shows that in spite of the geographical diversity, the infecting bacterial isolates and their antibiogram from this part are found to be alike to those found in any other part of Pakistan.

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Antibacterial resistance pattern in isolates from pus samples: An observational study

Ahmed J, Ali A, Hussain M, Ahmad H. 2013. Antimicrobial susceptibility pattern of bacteria isolated from burn wounds in a tertiary care hospital in Pakistan. African Journal of Microbiology Research 7(28), 3627-3631.

Bessa LJ, Fazii P, Di Giulio M, Cellini L. 2015. Bacterial isolates from infected wounds and their antibiotic susceptibility pattern: some remarks about wound infection. International wound journal 12(1), 47-52.

Bowler PG, Duerden BI, Armstrong DG. 2001. Wound microbiology and associated approaches to wound management. Clinical microbiology reviews 14(2), 244-469.

Cerceo E, Deitelzweig SB, Sherman BM, Amin AN. 2016. Multidrug-resistant gram-negative bacterial infections in the hospital setting: overview, implications for clinical practice, and emerging treatment options. Microbial Drug Resistance 22(5), 412-431.

CLSI. 2010. “Performance standards for antimicrobial susceptibility testing,” Twentieth informational supplement, Clinical and Laboratory Standards Institute Doc. M100eS20.

Cogen AL, Nizet V, Gallo RL. 2008. Skin microbiota: a source of disease or defence ?. British Journal of Dermatology 158(3), 442-455.

Dryden MS. 2009. Skin and soft tissue infection: microbiology and epidemiology. International Journal of Antimicrobial Agents 34, 2-7.

Dryden MS. 2010. Complicated skin and soft tissue infection. Journal of antimicrobial chemotherapy 65(3), 35-44.

Iredell J, Brown J, Tagg K. 2016. Antibiotic resistance in Enterobacteriaceae: mechanisms and clinical implications. Bmj 352, h6420.

Kehinde AO, Ademola SA, Okesola AO, Oluwatosin OM, Bakare RA. 2004. Pattern of bacterial pathogens in burn wound infections in Ibadan Nigeria. Annals of Burns and fire disasters 17(1), 12-15

Lockhart SR, Abramson MA, Beekmann SE, Gallagher G, Riedel S, Diekema DJ, Quinn JP, Doern GV. 2007. Antimicrobial resistance among Gram-negative bacilli causing infections in intensive care unit patients in the United States between 1993 and 2004. Journal of clinical microbiology 45(10), 3352-3359.

Mehta VJ, Kikani KM, Mehta SJ. 2014. Microbiological profile of diabetic foot ulcers and its antibiotic susceptibility pattern in a teaching hospital, Gujarat. Int J Basic Clin Pharmacol 3(1), 92-95.

Misic AM, Gardner SE, Grice EA. 2014. The wound microbiome: modern approaches to examining the role of microorganisms in impaired chronic wound healing. Advances in wound care 3(7), 502-510.

Prestinaci F, Pezzotti P, Pantosti A. 2015. Antimicrobial resistance: a global multifaceted phenomenon. Pathogens and global health 109(7), 309-318.

Rice LB. 2006. Antimicrobial resistance in gram-positive bacteria. American journal of infection control 34(5), 11-19.

Scalise A, Bianchi A, Tartaglione C, Bolletta E, Pierangeli M, Torresetti M, Marazzi M, Di Benedetto G. 2015. Microenvironment and microbiology of skin wounds: the role of bacterial biofilms and related factors. In Seminars in vascular surgery 28, 151-159.

Shahzad MN, Ahmed N, Qureshi KH. 2012. Reverse flow posterior interosseous flap: Experience with 53 flaps at Nishtar Hospital, Multan. Journal Pakistan Medical Association 62(9), 950-954.

Shriyan A, Sheetal R, Nayak N. 2010. Aerobic micro-organisms in post-operative wound infections and their antimicrobial susceptibility patterns. Journal of clinical and diagnostic research 4(6), 3392-3396.

Zhang S, Ren L, Li Y, Wang J, Yu W, Li N, Li J. 2014. Bacteriology and drug susceptibility analysis of pus from patients with severe intra‑abdominal infection induced by abdominal trauma. Experimental and therapeutic medicine 7(5), 1427-1431.

Saifullah Khan Jadoon, Kainat Saeed, Anwar Khalid, Naveed Muhammad, Palwasha Khan, Faheem Jan.
Antibacterial resistance pattern in isolates from pus samples: An observational study.
Int. J. Biosci. 15(4), 367-371, October 2019.
https://innspub.net/ijb/antibacterial-resistance-pattern-isolates-pus-samples-observational-study/
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