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Anticoagulant activity of salivary gland extract of a haematophagus insect, Rhipicephalus microplus from Bangladesh

By: Md. Forhad Hossain, A.S.M. Aminul Islam, Md Abu Reza, Khandaker Md. Khalid-Bin-Ferdaus

Key Words: Anticoagulant, Haematophagus insect, Salivary gland extract, Prothrombin time assay.

Int. J. Biosci. 13(3), 173-179, September 2018.

DOI: http://dx.doi.org/10.12692/ijb/13.3.173-179

Certification: ijb 2018 0001 [Generate Certificate]

Abstract

Blood sucking insect contains anticoagulant proteins or peptides in their saliva. In absence of such component in the saliva, insects will starve to death. These anticoagulants have remarkable biopharmaceutical applications in treatment of various thrombolytic disorders and other therapeutic indications. Therefore, these insects and their salivary gland is a virtual gold mine for drug lead compounds for future anticoagulant drug development. A number of research groups are currently involved in research on different haematophagus insects around the world. However, so far haematophagus insect of our region is totally unexplored. Therefore, it is interesting to search for anticoagulant activity of salivary gland extract (SGE) as a potential anticoagulant drug source from the blood sucking insects of our country. Among different tick species in Bangladesh Rhipicephalus microplus is the most common one. In the current project we (i) collected salivary glands from R. microplus, (ii) extracted protein from the salivary glands and (iii) examined anticoagulant activity of the salivary gland extract using prothrombin time (PT) assay. In comparison to control (14 sec), the salivary gland extract shows increased clotting time of 14.62 second, 16.42 second, 22.74 second, 26.18 second, 26.98 second and 27.04 second respectively in 2.5 ml, 5 ml, 7.5 ml, 10 ml, 12.5 ml and 15 ml concentration of SGE. Thus, R. microplus from Bangladesh has potential as a source of anticoagulant lead molecule. Further experimentation on isolation of anticoagulant molecule is in progress.

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Anticoagulant activity of salivary gland extract of a haematophagus insect, Rhipicephalus microplus from Bangladesh

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Md. Forhad Hossain, A.S.M. Aminul Islam, Md Abu Reza, Khandaker Md. Khalid-Bin-Ferdaus.
Anticoagulant activity of salivary gland extract of a haematophagus insect, Rhipicephalus microplus from Bangladesh.
Int. J. Biosci. 13(3), 173-179, September 2018.
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