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An antifungal phenazine pigment obtained from Pseudomonas aeruginosa inhibits the growth of Aspergillus ochraceus

By: Popy Devnath, Md. Kamal Uddin, Md. Faysal Kamal, Md. Towhid Hossain, Mohammed Abul Manchur

Key Words: Biocontrol, Pseudomonas, Pyocyanin.

Int. J. Biosci. 17(2), 140-148, August 2020.

DOI: http://dx.doi.org/10.12692/ijb/17.2.140-148

Certification: ijb 2020 0023 [Generate Certificate]

Abstract

Aspergillus ochraceus, a pathogenic mold frequently found in grains, soil, and dried food products. This mold can produce several toxins especially Ochratoxin A and B on infected crops. These toxins are associated with food intoxications both in humans and animals. This study aimed to evaluate the antifungal potentiality of a pigment produced by Pseudomonas aeruginosa, against A. ochraceus in-vitro. Primarily, the cross streak method showed P. aeruginosa (isolate PU8 and PU10) inhibits the growth of A. ochraceus. Then in the quantitative assay using crude extract of the isolate showed significant inhibitory (p<0.05) activities which were up to 72% inhibition by PU8 and 59% by PU10. Then the pigment was extracted, purified, and characterized. The UV spectrophotometry, FT-IR (Fourier Transform Infrared Spectrometry), TLC (Thin-Layer Chromatography) and microscopic analysis proved that the crude extract contained pyocyanin as a potent antifungal phenazine pigment. The adverse effects of chemical fungicides necessitated the use of eco-friendly biological control agents against fungi. Fortunately, from this study, we can infer that P. aeruginosa can produce an antifungal phenazine pigment pyocyanin that inhibits the growth of A. ochraceus and be used as a potential bio-control agent.

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An antifungal phenazine pigment obtained from Pseudomonas aeruginosa inhibits the growth of Aspergillus ochraceus

Bayman P, Baker JL, Doster MA, Michailides TJ, Mahoney NE. 2002. Ochratoxin Production by the Aspergillus ochraceus Group and Aspergillus alliaceus. Applied and environmental microbiology 68, 2326-2329.

https://doi.org/10.1128/AEM.68.5.2326

Bouhet S, Oswald IP. 2005. The effects of mycotoxins, fungal food contaminants, on the intestinal epithelial cell-derived innate immune response. Veterinary Immunology and Immunopathology 108, 199-209.

https://doi.org/10.1016/j.vetimm.2005.08.010

Devnath P, Uddin K, Ahamed F. Hossain TM, Manchur MA. 2017. Extraction, purification and characterization of pyocyanin produced by Pseudomonas aeruginosa and evaluation for its antimicrobial activity. International Research Journal of Biological Science 6, 1-9.

Dharni S, Alam M, Kalani K. 2012. Production, purification, and characterization of antifungal metabolite from Pseudomonas aeruginosa SD12, a new strain obtained from tannery waste polluted soil. Journal of Microbiology and Biotechnology 22, 674-683.

https://doi.org/10.4014/jmb.1109.09061

Dirheimer G, Creppy EE. 1991. Mechanism of action of ochratoxin A. IARC Scientific Publications 115, 171–186.

Essar DW, Eberly L, Hadero A, Crawford IP. 1990. Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: Interchangeability of the two anthranilate synthase and evolutionary implications. Journal of Bacteriology 172, 884-900.

Fischer G, Dott W. 2003. Relevance of airborne fungi and their secondary metabolites for environmental, occupational and indoor hygiene. Archives of Microbiology 1799, 75-82.

https://doi.org/10.1007/s00203-002-0495-2

Gniadek A. 2009. Cytotoxicity of Aspergillus Fungi as a Potential Infectious Threat. Insight and Control of Infectious Disease in Global Scenario, ed. R. Priti 12, 231–248. InTech. Croatia.

https://doi.org/10.5772/2240

Gupta PK. 2018. Toxicity of Fungicides. In: Veterinary Toxicology: Basic and Clinical Principles: Third Edition. Elsevier, © Academic Press 2018. https://doi.org/10.1016/b978-0-12-805426-0.00017-2

Gupta PK. 2016. In: Toxic effects of pesticides (agrochemicals). Fundamentals of Toxicology. Elsevier, © Academic Press 2016.

https://doi.org/10.1016/B978-0-12-811410-0.00045-3

Holt JH, Krieg NR, Sneath PH, Staley JT, Williams ST. 1994. Bergey’s manual of determinative bacteriology ninth edition. European journal of paediatric neurology 13, 560.

https://doi.org/10.1016/j.ejpn.2008.10.006

Jayaseelan S, Ramaswamy D, Dharmaraj S. 2014. Pyocyanin: production, applications, challenges and new insights. World Journal of Microbiology and Biotechnology 30, 1159-1168.

https://doi.org/10.1007/s11274-013-1552-5

Karpagam S, Sudhakar T, Lakshmipathy M. 2013. Microbicidal response of pyocyanin produced by Pseudomonas aeruginosa toward clinical isolates of fungi. International Journal of Pharmacy and Pharmaceutical Science 5, 870-873.

Kőszegi T, Poór M. 2016. Ochratoxin a: Molecular interactions, mechanisms of toxicity and prevention at the molecular level. Toxins (Basel) 8, 111.

https://doi.org/10.3390/toxins8040111

Marrez DA, Mohamad HS. 2020. Biological activity and applications of pyocyanin produced by Pseudomonas aeruginosa. Open Acces Journal of Biomedical Science 1, 140-144.

https://doi.org/10.38125/OAJBS.000133

Mavrodi D V, Blankenfeldt W, Thomashow LS. 2006. Phenazine compounds in fluorescent Pseudomonas spp. biosynthesis and regulation. Annual Review of Phytopathology 44, 417-445.

https://doi.org/10.1146/annurev.phyto.44.013106.145710

Morales DK, Grahl N, Okegbe C, Dietrich LE, Jacobs NJ, Hogan DA.  2013. Control of Candida albicans metabolism and biofilm formation by Pseudomonas aeruginosa phenazines. MBio 4, e00526-12.

https://doi.org/10.1128/mBio.00526-12

Moss MO. 2008. Fungi, quality and safety issues in  fresh fruits and vegetables. Journal of Applied Microbiology 104, 1239-1243.

https://doi.org/10.1111/j.1365-2672.2007.03705.x

Ohfuji K, Sato N, Hamada-Sato N. 2004. Construction of a glucose sensor based on a screen-printed electrode and a novel mediator pyocyanin from Pseudomonas aeruginosa. Biosensors and Bioelectronics 19, 1237-1244.

https://doi.org/10.1016/j.bios.2003.11.010

Özyürek SB, Gür SD, Bilkay IS. 2016. Investigation of Antimicrobial Activity of Pyocyanin Produced by Pseudomonas aeruginosa Strains Isolated from Different Clinical Specimens. Hacettepe Journal of Biology Chemistry 44, 1-6.

https://doi.org/10.15671/HJBC.20164417526

Pitt JI, Hocking AD, Pitt JI, Hocking AD. 1997. Aspergillus and Related Teleomorphs. In: Fungi and Food Spoilage. Springer, Boston, MA10.

https://doi.org/10.1007/978-0-387-92207-2_8

Someya N, Tsuchiya K, Yoshida T, Noguchi MT, Sawada H. 2006. Combined use of the biocontrol bacterium Pseudomonas fluorescens strain LRB3W1 with reduced fungicide application for the control of tomato fusarium wilt. Biocontrol Science 11, 75-80.

https://doi.org/10.4265/bio.11.75

Stuart BH. 2005. Infrared Spectroscopy: Fundamentals and Applications. John Wiley and Sons

Sudhakar T, Karpagam S. 2011. Antifungal efficacy of pyocyanin produced from bioindicators of nosocomial hazards. Proceedings of the International Conference on Green Technology and Environmental Conservation (GTEC-2011), Chennai 224-229.

https://doi.org/10.1109/GTEC.2011.6167673

Tournas VH. 2005. Spoilage of vegetable crops by bacteria and fungi and related health hazards. Critical Review of Microbiology 31, 33-44.

https://doi.org/10.1080/10408410590886024

Watson D, Macdermot J, Wilson R, Cole PJ, Taylor GW. 1986. Purification and structural analysis of pyocyanin and 1-hydroxyphenazine. European Journal of Biochemistry 159, 309-313.

https://doi.org/10.1111/j.1432-1033.1986.tb09869.x

Wu D, Huang W, Duan Q, Li F, Cheng H. 2014. Sodium houttuyfonate affects production of N-acyl homoserine lactone and quorum sensing-regulated genes expression in Pseudomonas aeruginosa. Frontiers of Microbiology 5, 635.

https://doi.org/10.3389/fmicb.2014.00635.

Popy Devnath, Md. Kamal Uddin, Md. Faysal Kamal, Md. Towhid Hossain, Mohammed Abul Manchur.
An antifungal phenazine pigment obtained from Pseudomonas aeruginosa inhibits the growth of Aspergillus ochraceus.
Int. J. Biosci. 17(2), 140-148, August 2020.
https://innspub.net/ijb/antifungal-phenazine-pigment-obtained-pseudomonas-aeruginosa-inhibits-growth-aspergillus-ochraceus/
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