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Antimicrobial and Immunomodulatory Properties of Crude Extract and Compounds from Boswellia dalzielii Hutch

By: Talom Tangue Benjamin, Tagne Simo Richard, Talla Emmanuel, Fodouop Chegaing Siméon Pierre, Njateng Guy Sedar Senghor, Nyemb Jean Noël, Almas Jabeen, Huma Aslam Bhatti, Rukesh Maharjan, Tatsadjieu NgouneLéopold, Kuiate Jules-Roger

Key Words: Boswellia dalzielii; antimicrobials; immunomodulatory; oxidative burst.

Int. J. Biosci. 14(2), 161-171, February 2019.

DOI: http://dx.doi.org/10.12692/ijb/14.2.161-171

Certification: ijb 2019 0132 [Generate Certificate]

Abstract

In the North of Cameroon, Boswellia dalzielii is a medicinal plant used in traditional medicine to treat infectious diseases (dysentery, typhoid, cholera and malaria). The aim of this study is to identify and characterized the bioactive compounds from the roots of this plant. The methanol crude extract from the root of Boswellia dalzielii Hutch was obtained by maceration in methanol. Partition of part of this crude extract using solvent-solvent extraction in n-hexane, ethyl acetate, n-butanol yield hexane, yield hexane, ethyl acetate, n-butanol and residual fractions. Ethyl acetate fraction showed the highest antibacterial activity and was subjected to column chromatography. Crude extract, fractions and pure compounds were tested for their antibacterial properties using microdilution method with Alamar Blue as dye. Immunomodulatory activities were determined using chemiluminescence immunoassay. Ethyl acetate fraction exhibited highest antimicrobial activities and its fractionation afforded 3 known compounds and a mixture: trans-desoxyrhapontigenin-3-O-rutinoside (1), α-amyrin (3) and trans-desoxyrhaponticin (4) and a mixture of β-amyrin and lupeol (2) in a ratio (1:2). Mixture (2) as far as compounds (1) and (3) showed no antimicrobial activity against all bacteria strains tested while desoxyrhapontigenin-3-O-rutincompound (4) (MIC=125µg/mL) was relatively active. Ethyl acetate fraction exhibited lowest immunomodulator activity while compound (3) showed highest immunomodulating activities towards polymorphoneutrophils (IC50 < 7 µg/mL). These results showed that Boswellia dalzielii possess both antibacterial and immunomodulatory effects due to different comounds and may justify it use in traditional medicine to fight infectious diseases.

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Antimicrobial and Immunomodulatory Properties of Crude Extract and Compounds from Boswellia dalzielii Hutch

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Talom Tangue Benjamin, Tagne Simo Richard, Talla Emmanuel, Fodouop Chegaing Siméon Pierre, Njateng Guy Sedar Senghor, Nyemb Jean Noël, Almas Jabeen, Huma Aslam Bhatti, Rukesh Maharjan, Tatsadjieu NgouneLéopold, Kuiate Jules-Roger.
Antimicrobial and Immunomodulatory Properties of Crude Extract and Compounds from Boswellia dalzielii Hutch.
Int. J. Biosci. 14(2), 161-171, February 2019.
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