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Antimicrobial susceptibility testing of ciprofloxacin, amoxicillin and erythromycin against Staphylococcus aureus and Escherichia coli isolated from healthy students of Delta State University Abraka South South, Nigeria

By: Anie Clement Oliseloke, Enwa Felix Oghenemaro, Jemikalaja Daniel Johnson, Okubuzor Benjamin

Key Words: Staphylococcus aureus, Escherichia coli, Resistance, Urine, Nostrils.

J. Bio. Env. Sci. 13(4), 158-165, October 2018.

DOI: http://dx.doi.org/10.12692/ijb/13.4.158-165

Certification: ijb 2018 0020 [Generate Certificate]

Abstract

Escherichia coli and Staphylococcus aureus have been identified to be amongst the key causes of hospital acquired infections worldwide. These microorganisms have been known for their high tendency to build up resistance to a wide variety of antibiotics that can render previously potent drugs ineffective. This study determined the frequency and susceptibility profile of Escherichia coli and Staphylococcus aureus nasal isolates from healthy students of Delta State University Abraka South South, Nigeria. The nasal and urine samples were collected and immediately inoculated on solidified Mannitol salt agar and on solid Mac Conkey agar respectively and incubated for 24 hours at 37°C. Ideal biochemical tests were carried out to identify each microorganism isolated. Out of one hundred (100) samples collected, 53 organisms were positively identified to be bacterial isolates.  Nineteen (38%) strains of S. aureus and 3 (6%) other S. spp were isolated.  Total number of Gram-negative bacteria from the urine samples is 32 bacterial isolates. Of the 32 predominant was twelve Proteus mirabilis (24%), followed by Citrobacter freundii (12%), and Aeromonas hydrophilia (12%), next is Proteus vulgaris(6%), E. coli isolates (4%) while Pseudomonas aeroginosa  (4%) and one strain of Alcaligenes feacalis (2%) was isolated. The antimicrobial resistance pattern shows that all the bacterial isolates had no resistance to ciprofloxacin. S. aureus showed some level of resistance to amoxicillin (10.5%) while erythromycin is(20 %). E coli showed complete resistance to amoxicillin (100%) and erythromycin (100%). Thus, it can be inferred that ciprofloxacin can be more effective in treating infections caused by the test microorganisms, than amoxicillin and erythromycin.

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Antimicrobial susceptibility testing of ciprofloxacin, amoxicillin and erythromycin against Staphylococcus aureus and Escherichia coli isolated from healthy students of Delta State University Abraka South South, Nigeria

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Anie Clement Oliseloke, Enwa Felix Oghenemaro, Jemikalaja Daniel Johnson, Okubuzor Benjamin.
Antimicrobial susceptibility testing of ciprofloxacin, amoxicillin and erythromycin against Staphylococcus aureus and Escherichia coli isolated from healthy students of Delta State University Abraka South South, Nigeria.
J. Bio. Env. Sci. 13(4), 158-165, October 2018.
https://innspub.net/ijb/antimicrobial-susceptibility-testing-ciprofloxacin-amoxicillin-erythromycin-staphylococcus-aureus-escherichia-coli-isolated-healthy-students-delta-state-university-abraka/
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