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Antimicrobial treatment of citrus budwood to control Candidatus Liberibacter asiaticus: An effort to develop low-cost technology for farmers

By: Muhammad Sarwar Yaqub, Rozina Aslam, Fatima Ismail, Azeem Nawaz Mughal, Muhammad Hassan, Afia Mushtaq

Key Words: Asian citrus psyllid, Citrus greening disease, Gram negative, Iodo starch test.

Int. J. Biosci. 17(3), 95-104, September 2020.

DOI: http://dx.doi.org/10.12692/ijb/17.3.95-104

Certification: ijb 2020 0036 [Generate Certificate]

Abstract

Huanglongbing (HLB), also known as citrus greening, is one of the most devastating diseases of citrus worldwide menacing the survival of Pakistan and the world’s citrus industry. HLB is caused by different species of Candidatus Liberibacter bacterium: Candidatus Liberibacter asiaticus, Candidatus Liberibacter americanus and Candidatus Liberibacter africanus. The bacterium is transmitted from one plant to another by psyllid vector and through budding/grafting of infected plant material. As potential control strategies for citrus HLB, the effectiveness of different doses of penicillin including 25ppm, 50ppm, 75ppm and 100ppm in solution form were evaluated on HLB infected scions grafted on a rough lemon. Infected grafts were immersed in penicillin solutions for 2, 4, 6 and 8 hours. Biochemical analysis for the elimination of bacterial pathogens from treated sprouts was performed using iodo starch test. Survival of disease-free scion after the treatment with the antimicrobial agent is a landmark for HLB management. Highly significant differences were obtained for Candidatus liberibacter asiaticus detection in penicillin treated grafts sprouts at 8 hours immersion periods (F= 42.12, P= 0.0001). Our results revealed that scions immersed for 8 hours in 75 ppm solution of penicillin provided the highest efficiency in graft survival, sprouting and suppressing the HLB. This may provide a useful tool for the management of citrus HLB for citrus growers.

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Antimicrobial treatment of citrus budwood to control Candidatus Liberibacter asiaticus: An effort to develop low-cost technology for farmers

Aubert B. 1993. Citrus greening disease, a serious limiting factor for citriculture in Asia and Africa. Proceeding of 6th Conference of the International Society of Nurserymen, South Africa. 134-142.

Aubert B. 1987. Trioza erytreae Del Guercio and Diaphorina citri Kuwayama (Homoptera: Psylloidea), the two vectors of citrus greening disease: biological aspects and possible control strategies. Fruits 42, 149-162.

Bove JM. 2006. Huanglongbing: a destructive, newly emerging, century old disease of citrus. Journal of Plant Pathology 88, 7-37. PMID: WOS: 000236591900002.

Brodersen C, Narciso C, Reed M, Etxeberria Ed. 2014. Phloem production in huanglongbing affected trees. Hort Science 49, 59-64.

Da Graca JV. 1991. Citrus greening disease. Annual Review of Phytopathology 29, 109–136.

Duan YP, Zhou LJ, Hall DG, Li WB, Doddapaneni H, Lin H, Liu L, Vahling CM, Gabriel DW, Williams KP, Dickerman A, Sun YJ, Gottwald T. 2009. Complete genome sequence of citrus huanglongbing bacterium, ‘Candidatus Liberibacter asiaticus’ obtained through metagenomics. Molecular Plant Microbe Interactions 22, 1011-1020.

http://aps journals.Apsnet.org/loi/mpmi

Etxeberria E, Gonzalez P, Achor D, Albrigo G. 2009. Anatomical distribution of abnormally high levels of starch in HLB-infected Valencia orange trees. Physiology and Molecular Plant Pathology 74, 76–83.  http://dx.doi.org/10.1016/j.pmpp.2009.09.004

Etxeberria E, Gonzalez P, Dawson W, Spann T. 2007. An iodine based starch test to assist in selecting leaves for HLB testing.

https://journals.flvc.org/edis/article/view/117218

Folimonova SY, Robertson CJ, Garnsey SM, Gowda S, Dawson WO. 2009. Examination of the responses of different genotypes of citrus to Huanglongbing (citrus greening) under different conditions. Phytopathology 99, 1346-1354.

http://dx.doi.org/10.1094/phyto-99-12-1346

Garnier M, Danel N, Bove JM. 1984. The organism is a gram-negative bacterium. In: Garnsey SM, Timmer LW, Dodds JA. (eds), Proc. 9th Conference of the International Organization of Citrus Virologists. Riverside, University of California: 115–124.

Gottwald TR. 2010. Current epidemiological understanding of citrus Huanglongbing. Annual Review of Phytopathology 48, 119-139.

https://doi.org/10.1146/annurev-phyto-073009-114418

Hodges AW, Spreen TH. 2006/7–2010/11. Economic impacts of citrus greening (HLB) in Florida. [Internet]. University of Florida Department of Food and Resource Economics. 2012. Available: http://news.ufl.edu/2012/01/24/greening-cost/.

Jagoueix S, Bove JM, Garnier M. 1994. The phloem-limited bacterium of greening is a member of the alpha subdivision of the proteobacteria. International Journal of Systematic Bacteriology 44, 379-386.

http://dx.doi.org/10.1099/00207713-44-3-379.

Jepson SB. 2009. Citrus greening disease (Huanglongbing). OSU Plant Clinic. Corvallis, Oregon, USA: Oregon State University.

Khan AH. 1989. Pathology of Trees. Faisalabad Press, University of Agriculture. 2, 47–49.

Li W, Hartung JH, Levy L. 2006. Quantitative real time PCR for detection and identification of Candidatus Liberibacter species associated with citrus huanglongbing. Journal of Microbiological Methods 66, 104-115.

http://dx.doi.org/10.1016/j.mimet.2005.10.018.

Lin KH. 1956. Observation of yellow shoot on citrus.  Etiological studies of yellow shoot on citrus. Acta Phytopathologica Sinica 2, 1-42.

McClean APD, Schwarz RE. 1970. Greening of blotchy-mottle disease of citrus. Phytophylactica 2, 177-94.

McManus PS, Stockwell VO, Sundin GW, Jones AL. 2002. Antibiotic use in plant agriculture. Annual Review of Phytopathology 40, 443-465. https://doi.org/10.1146/annurev.phyto.40.120301.093927

Miyakawa T. 1980. Experimentally-induced symptoms and host range of citrus likubin (greening disease). Annals of Phytopathological Society of Japan 46, 224–230.

Nariani TK, Ghosh SK, Kumar D, Raychaudhuri SP, Wishwanath SM. 1975. Detection and possibilities of therapeutic control of the greening disease of citrus caused by mycoplasma. Proceeding of Indian Natural Science Academic Series B 41, 334-339.

Nariani TK, Raychaudhuri SP, Wishwanath SM. 1971. Response of greening pathogen of citrus to certain tetracycline antibiotics. Current Science 20, 552.

Sagaram M, Burns JK. 2009. Leaf Chlorophyll Fluorescence Parameters and Huanglongbing. Journal of American Society of Horticultural Sciences 134(2), 194-201.

https://doi.org/10.21273/JASHS.134.2.194

Spratt BG, Cromie KD. 1988. Penicillin-binding proteins of gram-negative bacteria. Reviews of Infectious Diseases 10, 699-711.

Stansly P, Arevalo H, Qureshi j, Jones M, Hendricks K, Roberts P, Roka F. 2014. Vector control and foliar nutrition to maintain economic sustainability of bearing citrus in Florida groves affected by Huanglongbing. Pest Management Science 70, 415-426.

http://dx.doi.org/10.1002/ps.3577

Su HJ, Chen CN. 1990. Implementation of IPM on citrus virus and greening (likubin) disease. Proceeding of International workshop TARI, Taichung, Taiwan, April 9-14.

Taba S, Nasu K, Takaesu K, Ooshiro A, Moromizato ZI. 2006. Detection of citrus huanglongbing using an iodo starch reaction 53, 19-23.

Teixeira DC,Danet JL, Eveillard S, Martins EC, Junior WCJ, Yamamoto PT, Lopes SA, Bassanezi RB, Ayers AJ, Sailard C, Bove JM. 2005. Citrus huanglongbing in Sao Paulo State, Brazil: PCR detection of the Candidatus Liberibacter species associated with the disease. Molecular and Cellular Probes 19, 173-179.

Timmer LW, Garnsey SM, Broadbent P. 2003. Diseases of citrus pp.163-195. In: Ploetz RC (ed.). Disease of Tropical Fruit Crops. CABI Publishing, USA.

Weinert MP, Jackson SC, Grimshaw JF, bellis GA, Stephen PM, Gunua TG, Kame MF, Davis RI. 2004. Disease notes and new records. Detection of Huanglongbing (citrus greening disease) in Timor-Leste (East Timor) and in Papua New Guinea. CRISO Publishing. Australian Plant Pathology 33, 135–136.

Yang C, Powell CA, Duan Y, Shatters R, Zhang M. 2015. Antimicrobial nano emulsion formulation with improved penetration of foliar spray through citrus leaf cuticles to control citrus huanglongbing. PloS one 10, e0133826.

Yaqub MS, Khan IA, Aslam R. 2019. Antibiotic treatment of citrus budwood for the management of huanglongbing disease. International Journal of Biosciences 15, 99-109.

http://dx.doi.org/10.12692/ijb/15.2.99-109

Zhang M, Guo Y,Powell CA, Doud MS, Yang C, Duan Y. 2014. Effective antibiotics against Candidatus Liberibacter asiaticus in HLB-affected citrus plants identified via the graft-based evaluation. PLoS One 9(11), e111032.

http://dx.doi.org/10.1371.

Muhammad Sarwar Yaqub, Rozina Aslam, Fatima Ismail, Azeem Nawaz Mughal, Muhammad Hassan, Afia Mushtaq.
Antimicrobial treatment of citrus budwood to control Candidatus Liberibacter asiaticus: An effort to develop low-cost technology for farmers.
Int. J. Biosci. 17(3), 95-104, September 2020.
https://innspub.net/ijb/antimicrobial-treatment-citrus-budwood-control-candidatus-liberibacter-asiaticus-effort-develop-low-cost-technology-farmers/
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