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Antioxidant activity of organic extracts of root bark of Ziziphus jujube Gaertn (L) var. hysudrica Edgew

By: Dure-E-Shahwar, Khalid Hussain, Nadeem Irfan Bukhari, Zafar Iqbal, Ahmad Adnan, Naureen Shahzadi, Freeha Rafique

Key Words: Root bark, Extraction, Total phenolic content, Total flavonoid content, Antioxidant activity

Int. J. Biosci. 15(2), 559-569, August 2019.

DOI: http://dx.doi.org/10.12692/ijb/15.2.559-569

Certification: ijb 2019 0011 [Generate Certificate]

Abstract

Extracts of root bark of ziziphus jujube were prepared by using methanol, acetone, ethyl acetate, dichloromethane, chloroform and n-Hexane through maceration. Their total phenolic contents and total flavonoid contents were determined by using single concentration of all extracts (200µg/mL). Total phenolic contents 207.33, 197.33, 157.33, 97.33, 67.33mg eq GA/g were measured in methanol, dichloromethane, acetone, chloroform, ethyl acetate and n-hexan respectively. While total flavonoid contents 162.28, 162.28, 138.59, 133.33, 128.54 and 93,85mg eq rutin/g were measured in acetone, ethyl acetate, chloroform, dichloromethane, n-hexane and methanol extract respectively. Antioxidant activity of organic extracts of root bark of Ziziphus jujube was measured by three methods; DPPH, TAC and ABTS assays. Highest antioxidant activity was observed for dichloromethane extract (DPPH 41.26%, ABTS 47.64% and TAC 504.87mg ascorbic acid equivalent/g of extract) and methanol extracts (DPPH 40.14%, ABTS 48.69% and TAC 501.21mg ascorbic acid equivalent/g of extract). The results of antioxidant potential can be clearly related to total phenolic content results as both dichloromethane and methanol extracts were high in total phenolics and have shown high antioxidant potential. A Dose dependent study was conducted by using DPPH and ABTS assays for determining IC50 value. Data was analyzed by using one-way ANOVA. Comparison was performed by using LSD test and p ˂ 0.05 was considered as significant.

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Antioxidant activity of organic extracts of root bark of Ziziphus jujube Gaertn (L) var. hysudrica Edgew

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Dure-E-Shahwar, Khalid Hussain, Nadeem Irfan Bukhari, Zafar Iqbal, Ahmad Adnan, Naureen Shahzadi, Freeha Rafique.
Antioxidant activity of organic extracts of root bark of Ziziphus jujube Gaertn (L) var. hysudrica Edgew.
Int. J. Biosci. 15(2), 559-569, August 2019.
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