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Carbon source and lifting of recalcitrance to the induction of somatic embryos in cacao (Theobroma cacao L.)

By: Koné Daouda, N’Nan Alla Oulo, Kouassi Kan Modeste, Koffi Kouablan Edmond

Key Words: Key words: Recalcitrant, Cocoa, Somatic embryogenesis, Glucose, Fructose, Sucrose.

Int. J. Biosci. 19(1), 75-84, July 2021.

DOI: http://dx.doi.org/10.12692/ijb/19.1.75-84

Certification: ijb 2021 0260 [Generate Certificate]

Abstract

In cocoa trees, genetic improvement has allowed the development of elite high-producing varieties. Unfortunately, the plant’s natural method of multiplication comes up against certain difficulties which do not allow sufficient improved material to be available and to have homogeneous plantings. This work aimed to develop a method for mass production by in vitro culture of improved materials deemed recalcitrant to somatic embryogenesis. Different concentrations of glucose, sucrose and fructose were used to induce the production of embryos of four improved and recalcitrant genotypes to somatic embryogenesis coded C8, C14, C15 and C16. The plant material consisted of petal and staminode explants. The best results which are characterized by levels of callus embryogenesis and the number of somatic embryos of 30% and 20, respectively, were obtained with glucose at a concentration of 30 g/L. This study has shown that it is possible to produce somatic embryos in these improved varieties, thus removing the recalcitrance of these genotypes to somatic embryogenesis. The availability of improved material, consisting of identical plants, will allow the creation of more homogeneous plantations with high yields.

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Carbon source and lifting of recalcitrance to the induction of somatic embryos in cacao (Theobroma cacao L.)

Alemanno L, Ramos T, Gargadenec A, Andary C, Ferriere N.  2003. Localization and identification of phenolic compound in Theobroma cacao L. somatic embryogenesis. Annals of Botany 92(4), 613-623.

Borkowska B, Szczerba J. 1991. Influence of different carbon sources on invertase activity and growth of sour cherry (Prunus cerasus L.) shoot cultures. Journal of Experimental Botany 42(240), 911-915.

Brown DCW, Finstad KI, Watson EM. 1995. Somatic embryogenesis in herbaceous dicots. In: In vitro Embryogenesis in plants. (T. A. Thorpe-Edition.). Amsterdam Kluwer Academic Publishers, p 345-415.

Chauvin JE, Salesses G. 1988. Effet du fructose sur la micropropagation du châtaignier Castanea sp. Academic Sciences 306(5),  207-212.

Driver JA, Kuniyuki AH. 1984. In vitro propagation of paradox walnut root stock. HortScience 19, 507-509.

Eliane MT, Modeste KK, André SB, Edmond KK, Mongomaké K. 2019. Effect of Water Stress Induced by Polyethylene Glycol 6000 on Somatic Embryogenesis in Cocoa (Theobroma cacao L.). Agricultural Sciences 10, 1240-1254.

Gill R, Saxena PK. 1993. Direct somatic embryogenesis and regeneration of plants from seedlings explant of peanut (Arachis hypogaea L.) promotive role of thidiazuron can. Journal. Botany, 70, 1186–1192.

Hitomi K, Chokyu S, Ikeda Y. 2019. Micropropagation of Asparagus through somatic embryogenesis. 1. Somatic embryogenesis and plant regeneration from seedlings. Hiroshima Prefect Agriculture Expedition 53, 43-50.

Issali AE, Traoré A, Koffi KE, Ngoran JAK, Sangaré A. 2008a. Characterization of callogenic and embryogenic abilities of some genotypes of cocoa (Theobroma cacao L.) under selection in Côte d’Ivoire. Biotechnology Egypt, 7, 51-58.

http://dx.doi.org/10.3923/biotech.2008.51.58

Jane K, Siaka K, Lucien D, Georges N. 2017. Enhanced Plantlet Regeneration in Two Cacao (Theobroma cacao) Clones from Immature Inflorescence Explants. Hortsciences 52(6), 892–895. 2017.

http://dx.doi.org/10.21273/HORTSCI11844-17

Koné D, Kouassi KM, N’Nan AO, Kouablan KE. 2019. Induction of somatic embryos of recalcitrant genotypes of Theobroma. Journal of Applied Biosciences 133, 13552 – 13560.

Koné D, N’Nan AO, Kouassi KM, Kouablan K E. 2021. Use of mineral salts to remove recalcitrance to somatic embryogenesis of improved genotypes of cacao (Theobrama cacao L.). African Journal of Biotechnology 20(1), 33-42.

Kouassi KM, Manlé TE, Koné D, Soumahoro A B, Koné T, Kouablan KE, Koné M. 2017a. Effect of antioxidants on the callus induction and the development of somatic embryogenesis of cocoa [Theobroma cacao (L.)]. Australian Journal of Crop Sciences 11(1), 25-31.

https://doi.org/10.21475/ajcs.2017.11.01.pne174

Kouassi KM, Kahia J, Kouame NC, Tahi GM, Kouablan KE. 2017b. Comparing the effect of plant growth regulators on callus and somatic embryogenesis induction in four elite Theobroma cacao L. Genotypes Hortsciences 52(1), 142–145. http://dx.doi.org/10.21273/HORTSCI11092-1.6

Kouassi KM, Kouablan KE, Silué O, Tahi GM, Touré M,  Konan KP. 2018. Comparison of systems combining auxins with thidiazuron or kinetin supplemented with polyvinylpirrolidone during embryogenic callus induction in three Theobroma cacao L. genotypes. Internatinal Journal Biological and Chemical Sciences 12(2), 804-811.

http://dx.doi.org/10.4314/ijbcs.v12i2.15

Li Z, Traoré A, Maximova S, Guiltinan MJ. 1998. Somatic embryogenesis and plant regeneration from floral explants of cacao (Theobroma cacao L.) using thidiazuron. In Vitro Cellular Development Biology Plant 34, 293-299.

http://dx.doi.org/10.1007/BF02822737

Micheli F, Guiltinan M, Gramacho KP, Wilkinson MJ, Figueira AVDO, Cascardo JC DM, Maximova S, Lanaud C. 2009. Functional genomics of cacao. Advances Botony Research, 119-177.

Minyaka E, Niemenak N, Ngangue LTA, Madina BCV, Bahoya JA, Omokolo ND. 2017. Peroxidase and polyphenol oxidase activities associated to somatic embryogenesis potential in an elite hybrid genotype of Theobroma cacao L. African Journal of Biotechnology 16(49), 2278-2288. http://dx.doi.org/10.5897/AJB2017.16157

Moncousin C, Ribaux M, O’Rourke J, Gavillet S. 1992. Effects of type of carbohydrate during proliferation and rooting of microcuttings of MalusJork 9. Agronomie 12(10), 775-781.

Tahi GM, Trebissou IC, Guiraud SB, Ribeyre F, Lachenaud P, Pokou ND, N’guessan KF, Walet P N, Coulibaly K, Kébé IB, Assi MEB,  Koné B, Kassin E, Cilas C. 2017. Second cycle de sélection récurrente du cacaoyer (Theobroma cacao L.) en Côte d’Ivoire : Genetic parameters in the two constitutive 2, 47-67.

Tan CL, Furtek DB. 2003.  Development of an in vitro regeneration system for Theobroma cacao from mature tissues. Plant Sciences 164, 407-412.

Traoré A, Guiltinan MJ. 2006. Effects of carbon source and explants type on somatic embryogenesis of four cacao genotypes. Horticultural Sciences, 41(3), 753-758.

Koné Daouda, N’Nan Alla Oulo, Kouassi Kan Modeste, Koffi Kouablan Edmond.
Carbon source and lifting of recalcitrance to the induction of somatic embryos in cacao (Theobroma cacao L.).
Int. J. Biosci. 19(1), 75-84, July 2021.
https://innspub.net/ijb/carbon-source-and-lifting-of-recalcitrance-to-the-induction-of-somatic-embryos-in-cacao-theobroma-cacao-l/
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