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Characterization of Lasiodiplodia pseudotheobromae associated with citrus stem-end rot disease in Bangladesh

By: Razia Sultana, Md. Samiul Islam, Hafizur Rahman, Md. Shah Alam, Md. Asadul Islam, Biswanath Sikdar

Key Words: Stem-end rot, citrus fruit, Lasiodiplodia pseudotheobromae, molecular identification, bio-control.

Int. J. Biosci. 13(5), 252-262, November 2018.

DOI: http://dx.doi.org/10.12692/ijb/13.5.252-262

Certification: ijb 2018 0029 [Generate Certificate]

Abstract

Stem-end rot is the most common postharvest citrus fruit diseases in Bangladesh and worldwide. The aim of the present study was to identify the causal pathogen and assays to in vitro control of the pathogen against different plant extracts. The pathogen was isolated from infected fruit on PDA media forming white to grey colonies. The maximum mycelial growth of isolate was recorded at temperature 250C and 300C with radial growth 88.00 mm and 79.00 mm respectively on PDA media. Different pH, carbohydrates, and salts were also used to lead the growth profiling which also revealed the morphology of isolated pathogen. The isolated fungus was identified based on a combination of morphological characteristics and sequencing of the internal transcribed spacers (ITS) region and the gene encoding lp1 small subunit ribosomal RNA gene. The nucleotide sequence comparison and phylogenetic analyses indicate approximately 99% identity with the fungal pathogen Lasiodiplodia pseudotheobromae. Based on the morphological characteristics, molecular and phylogenetic approaches, the causal fungus was identified as L. pseudotheobromae. Different plants extract were used to evaluate in vitro control of the citrus fruit end-rot disease pathogen. The methanolic extract of Azadirachta indica and Psidium guajava significantly inhibited the mycelial growth (93.78% and 90.90% respectively) of L. pseudotheobromae in vitro.

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Characterization of Lasiodiplodia pseudotheobromae associated with citrus stem-end rot disease in Bangladesh

Al-Adawi AO, Deadman ML, Al-Rawahi AK, Khan AJ, Al-Maqbali YM. 2003. Diplodia theobromae associated with sudden decline of mango in the Sultanate of Oman. Plant Pathology 52, 419-425.

https://doi.org/10.1046/j.1365-3059.2003.00841.x

Alves A, Crous PW, Correia A, Phillips AJL.  2008. Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. Fungal Diversity 28, 1-13.

Bashir U, Tahira JJ. 2012. Evaluation of Eucalyptus camaldulensis against Fusarium solani. International Journal of Agriculture & Biology 14, 53-55.

Brouwer H, Coutinho PM, Henrissat B, de Vries RP. 2014. Carbohydrate-related enzymes of important Phytophthora plant pathogens. Fungal Genetics Biology 72, 192-200.

https://doi.org/10.1016/j.fgb.2014.08.011

Burgess TI, Barber PA, Mohali S, Pegg G, de Beer W, Wingfield MJ. 2006. Three new Lasiodiplodia spp. from the tropics, recognized based on DNA sequence comparisons and morphology. Mycologia 98, 423-435.

https://doi.org/10.1080/15572536.2006.11832677

Chakraborty U, Dutta SK, Chakraborty BN. 1991. Antifungal activity of some plant extracts on phytopathogenic fungi. Indian Botanical Contactor 8, 107-111.

Chen S, Li G, Liu Q, Li J, Liu F. 2016. Characteristics of Lasiodiplodia theobromae from Rosa rugosa in South China. Crop Protection 79, 51-55.

https://doi.org/10.1016/j.cropro.2015.10.011

Coutinho IBL, Freire FCO, Lima CS, Lima JS, Gonçalves FJT, Machado AR, Cardoso JE. 2017. Diversity of genus Lasiodiplodia associated with perennial tropical fruit plants in northeastern Brazil. Plant Pathology 66, 90-104.

https://doi.org/10.1111/ppa.12565

Dhandhukia PC, Thakkar VR. 2007. Standardization of growth and fermentation criteria of Lasiodiplodia theobromae for production of jasmonic acid. African Journal of Biotechnology 6,  707-712.

Droby S, Cohen L, Daus A, Weiss B, Horev B, Chalutz E, Katz H, Keren-Tzur M, Shachnai A. 1998. Commercial testing of Aspire: a yeast preparation for the biological control of postharvest decay of citrus. Biological Control 12, 97-101.

https://doi.org/10.1006/bcon.1998.0615

Felix C. Duarte AS. Vitorino R, Guerreiro AC. Domingues P. Correia A, Esteves AC. 2016. Temperature modulates the secretome of the phytopathogenic fungus Lasiodiplodia theobromae. Frontiers in Plant Science 7, 1096-1100.

https://doi.org/10.3389/fpls.2016.01096

Fong YK, Anuar S, Lim HP, Tham FY, Sanderson FR. 2000. A modified filter paper technique for long-term preservation of some fungal cultures. Mycologist 14, 127-130.

https://doi.org/10.1016/S0269-915X(00)80090-7

Fulton RW. 1986. Practices and precautions in the use of cross protection for plant virus disease control. Annual Review of Phytopathology 24, 67-81.

Islam MS, Sultana R, Khalekuzzaman M, Sikdar B, Acharjee UK, Hasan MF, Islam MA. 2017. Isolation and characterization of bacterial spot disease of citrus through biochemical approaches and its control measures. Journal of Pharmacognosy and Phytchemistry 6, 2418-2422.

Ismail M, Zhang J. 2004. Post-harvest citrus diseases and their control. Outlooks Pest Management 15, 29-44.

Jacobs KA, Rehner SA. 1998. Comparison of cultural and morphological characters and ITS sequences in anamorphs of Botryosphaeria and related taxa. Mycologia 90, 601-610.

https://www.jstor.org/stable/3761219

Kader AA, Rolle RS. 2004. The role of post-harvest management in assuring the quality and safety of horticultural produce; June 23-34; Rome, Italy: Food & Agriculture Organization.

Kour A, Shawl AS, Rehman S, Sultan P, Qazi PH, Suden P, Verma V. 2008. Isolation and identification of an endophytic strain of Fusarium oxysporum producing podophyllotoxin from Juniperus recurva. World Journal of Microbiology and Biotechnology 24, 1115-1121.

https://doi.org/10.1007/s11274-007-9582-5

Lee SF, Forsberg CW, Gibbins LN. 1985. Cellulolytic activity of Clostridium acetobutylicum. Applied Journal of Environmental Microbiology 50, 220-228.

Meszka B, Michalecka M. 2016. Identification of Phytophthora sp. isolated from plants and soil samples on strawberry plantations in Poland. Journal of Plant Diseases and Protection 123, 29-36.

https://doi.org/10.1007/s41348-016-0007-2

Munirah MS, Azmi AR, Yong SYC, Nur Ain Izzati MZ. 2017. Characterization of Lasiodiplodia theobromae and L. pseudotheobromae  causing fruit rot on pre-harvest mango in Malaysia. Plant Pathology and Quarantine 7, 202–213.

Punnithaligam E. 1980. Plant diseases attributed to Botryodiplodia theobromae Pat. Mycologia 71, 1-123.

Ram L, Singh S. 2006. Medicinal importance of citrus products and by-products-a review. Agricultural Reviews. 27, 170-180.

Rangaswami G. 1958. An agar blocks technique for isolating soil micro-organisms with special reference to Pythiaceous fungi. Science and Culture 24, 20-26.

Rastogi RP, Mehrotra BN. 1995. Compendium of Indian Medicinal Plants. May 3-4; New Delhi, CDRI, Lucknow, India: Publications & Information Directorate.

Rieger M. 2006. Introduction to fruit crops 4, USA: The Haworth Press, p 87-90.

Saha D, Dasgupta S, Saha A. 2005. Antifungal activity of some plant extracts against fungal pathogens of tea (Camellia sinensis.). Pharmaeutical Biology 43, 87-91.

https://doi.org/10.1080/13880200590903426

Sarker MNI, Barman SC, Islam MM, Islam MR, Chakma AS. 2017. Role of lemon (Citrus limon) production on livelihoods of rural people in Bangladesh. Journal of Agricultural Economics and Rural Development 3, 167-175.

Sathya K, Parthasarathy S, Thiribhuvanamala G, Prabakar K. 2017. Morphological and molecular variability of Lasiodiplodia theobromae causing stem end rot of mango in Tamil Nadu, India. International Journal of Pure and Applied Bioscience 5, 1024-1031.

Sharma P, Sharma RA, Vyas GK. 2013. Comparative antimicrobial activity and phytochemical analysis of Datura stramonium L, plant extracts and callus In vitro. European Journal of Medicinal Plants 3, 281-287.

Spiegel-Roy P, Goldschmidt EE. 1996. The biology of citrus. 1, UK: Cambridge University Press, 369-374.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30, 2725-2729.

https://doi.org/10.1093/molbev/mst197

Ullah SF, Hussain Y, Iram S. 2017. Pathogenic characterization of Lasiodiplodia causing stem end rot of mango and its control using botanics. Pakistan Journal of Botany 49, 1605-1613.

Vincent JM. 1947. Distortion of fungal hyphae in the presence of certain inhibitors. Nature 159, 850-860.

https://doi.org/10.1038/159850b0

Woo PC, Lau SK, Ngan AH, Tse H, Tung ET,  Yuen KY. 2008. Lasiodiplodia theobromae pneumonia in a liver transplant recipient. Journal of Clinical Microbiology 46, 380-384.

https://doi.org/10.1128/JCM.01137-07

Zhang J. 2007. The potential of a new fungicide fludioxonil for stem-end rot and green mold control on Florida citrus fruit. Postharvest Biology and Technology 46, 262-270.

https://doi.org/10.1016/j.postharvbio.2007.05.016

Zhang J, Swingle PP. 2005. Effects of curing on green mold and stem-end rot of citrus fruit and its potential application under Florida packing system. Plant Disease 89, 834-840.

https://doi.org/10.1094/PD-89-0834

Razia Sultana, Md. Samiul Islam, Hafizur Rahman, Md. Shah Alam, Md. Asadul Islam, Biswanath Sikdar.
Characterization of Lasiodiplodia pseudotheobromae associated with citrus stem-end rot disease in Bangladesh.
Int. J. Biosci. 13(5), 252-262, November 2018.
https://innspub.net/ijb/characterization-lasiodiplodia-pseudotheobromae-associated-citrus-stem-end-rot-disease-bangladesh/
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