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Characterization of Zoonotic Bacteria from Captive Crocodiles in Bangladesh

By: Ariful Islam, Md. Bakhtiar Lijon, Md. Mahadee Hasan, Abu Syem Muhammad Arif, Mahbubul Pratik Siddique, Muhammad Tofazzal Hossain , Md. Ariful Islam

Key Words: Antibiogram, Bacterial flora, Bangladesh, Crocodiles, PCR.

Int. J. Biosci. 19(2),139-152, August 2021.

DOI: http://dx.doi.org/10.12692/ijb/19.2.139-152

Certification: ijb 2021 0275 [Generate Certificate]

Abstract

An attempt was undertaken to characterize the bacterial flora of saltwater crocodiles reared on a commercial farm at Valuka, Mymensingh, Bangladesh. Swab samples (n=60) comprised of the oral swab (n=20), nasal swab (n=20) and cloacal swab (n=20) were collected from apparently healthy farm-raised crocodiles. Samples were cultured into various selective media to isolate bacteria. Characterization of the isolated bacteria was performed by studying the cultural, staining, biochemical properties followed by polymerase chain reaction. The antimicrobial susceptibility patterns of bacteria were investigated against eight commonly used antibiotics by the disc diffusion method. A total of 135 bacterial isolates belonged to 5 genera such as Escherichia (22%), Salmonella (22%), Staphylococcus (21%), Bacillus (16%) and Vibrio (19%) were identified. A genus-specific PCR targeting 16s rRNA gene successfully identified Escherichia coli, Salmonella spp. and Staphylococcus spp.  In PCR assay, 11 of 30 (36.67%) E. coli isolates were found positive for stx1 and stx2 genes and 4 of 30 (13.33%) isolates were found positive for and hly gene. Five isolates of Staphylococcus spp. were found positive with nuc gene, indicating these were pathogenic strains of Staphylococcus aureus. Multidrug resistance profile was observed in E. coli, Salmonella spp. and Vibrio spp. The results of this study indicate that captive crocodiles in the farm harbor multidrug-resistant pathogenic bacteria, which may produce infection in crocodiles and might cause health problems if transmitted to a human.

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Characterization of Zoonotic Bacteria from Captive Crocodiles in Bangladesh

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Ariful Islam, Md. Bakhtiar Lijon, Md. Mahadee Hasan, Abu Syem Muhammad Arif, Mahbubul Pratik Siddique, Muhammad Tofazzal Hossain , Md. Ariful Islam.
Characterization of Zoonotic Bacteria from Captive Crocodiles in Bangladesh.
Int. J. Biosci. 19(2),139-152, August 2021.
https://innspub.net/ijb/characterization-of-zoonotic-bacteria-from-captive-crocodiles-in-bangladesh/
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