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A comparative study of total phenolic contents and antioxidant potential of seeds of Peganum harmala

By: Peganum harmala L., Methanol, Dichloromethane, Extraction, Total phenolic content, Antioxidant activity.

Key Words: Peganum harmala L., Methanol, Dichloromethane, Extraction, Total phenolic content, Antioxidant activity.

Int. J. Biosci. 14(3), 431-437, March 2019.

DOI: http://dx.doi.org/10.12692/ijb/14.3.431-437

Certification: ijb 2019 0032 [Generate Certificate]

Abstract

Various solvents including methanol, hexane, benzene, chloroform and dichloromethane were utilized for the extraction of phenolic contents from the seeds of pegnum harmala. Total phenolic contents (TPC) were determined by using Folin-Ciocalteu reagent method against gallic acid as standard by UV-Vis spectrophotometer at 765 nm while DPPH  free radical scavenging activity of the extracts was measure by using UV-Vis spectrophotometer and taking reading at 517 nm and ascorbic acid as standard. Total phenolic contents 27.7 mg GA/g, 22.2 mg GA/g, 26.4 mg GA /g, 30.7 mg GA /g and 17.3 mg GA /g were for dichloromethane, benzene, chloroform, methanol and hexane extracts respectively. Methanol extract showed high TPC content and high antioxidant activity (72%) followed by dichloromethane extract (67%), chloroform extract (63 %), benzene extract (52 %) and hexane extract (48 %) respectively for 50 μL of each sample. The antioxidant activity was concentrated dependent for all the solvents and high TPC contents showed higher antioxidant activity.

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A comparative study of total phenolic contents and antioxidant potential of seeds of Peganum harmala

Asgarpanah J, Ramezanloo F. 2012. Chemistry, pharmacology and medicinal properties of Peganum harmala L. African Journal of Pharmacy and Pharmacology 6, 1573-1580.

Abdullahi MN, Ilyas N, Ibrahim H. 2013.Evaluation of phytochemical screening and analgesic activity of aqueous extract of the leaves of Microtrichiaperotitiidc (Asteraceae) in mice using hotplate method. Journal of Medicinal Plant Research 3, 37-43.

Ayoola GA, Coker HB, Adesegun SA, Adepoju AA, Obbayweya K, Ezennia EC, Atangbayila TO. 2008.  Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in southwestern Nigeria. Tropical Journal of Pharmaceutical Research 7, 1019-1024.

Banso A, Adeyemo S. 2006. Phytochemical screening and antimalarial assessment of Abutilon mauritiamum, Bacopamonnifera and Daturastramonium. Biokemistri 18, 39-44.

Bhalodia NR, Shukla VJ. 2015. Antibacterial and antifungal activities from leaf extracts of Cassia fistful L.: An ethnomedicinal plant. Journal of Advanced Pharmaceutical Technology and Research 2, 104-108.

Baiano A, del Nobile MA. 2015. Antioxidant compounds from vegetable matrices: Biosynthesis, occurrence, and extraction systems. Critical Reviews of Food Science and Nutrition 56, 2053–2068.

Djarmoun M, Boumerfeg S, Baghiani A, Boussoualim N, Zerargui F, Trabsa H, Belkhiri F, Khennouf S, Arrar L. 2012.  Evaluation of antioxidant and antibacterial properties of peaganum harmala seed extracts.  Research. Journal of Pharmaceutical, Biological and Chemical Sciences 3(4), 1109-1120.

Djarmoun M, Boumerfeg S, Baghiani A, Boussoualim N, Zerargui F, Trabsa H, Belkhiri F, Khennouf S, Arrar L. 2012.  Evaluation of antioxidant and antibacterial properties of peaganum harmala seed extracts. Research. Journal of Pharmaceutical, Biological and Chemical Sciences 3(4), 1109-1120.

Espín JC, Soler-Rivas C, Wichers HJ. 2000. Characterization of the total free radical scavenger capacity of vegetable oils and oil fractions using 2, 2-diphenyl-1-picrylhydrazyl radical. Journal of Agricultural and Food Chemistry 48, 648-656.

Fang YZ, Yang S, Wu G. 2002. Free radicals, antioxidants, and nutrition. Nutrition 18, 872–879.

Joshi A, Bhobe M, Saatarkar A. 2013. Phytochemical investigation of the roots of Grewiamicrocos Linn. Journal of Chemical and Pharmaceutical Research 5, 80-87.

Iqbal E, Salim KA, Lim LBL. 2015. Phytochemical screening, total phenolic and antioxidant activities of bark and leaf extracts of Goniothalamudvelutinus (Airy Shaw) from Brunei Darussalam. Journal of King Saud University 27, 224-232.

Kaskoos RA. 2014. Physico-chemical parameters, phytochemical screening and antioxidant activity of seeds of Peganum harmala collected from Iraq. Asian Journal of Biomedical and Pharmaceutical Sciences 4, 20-24.

Klancnik A, Piskernik S, Jersek B, Mozina SS. Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts. Journal of microbiological methods 81, 121-126.

Khademalhosseini AA, Tabatabaei A, Akbari  P, Fereidouni MS, Akhlaghi M. 2015. Comparison of in vivo antiseptic and in vitro antimicrobial effects of Peganum harmala L. seeds ethanolic extract with Butadiene. Journal of Coastal Life Medicine 3, 70-77.

Leamsomrong K, Suttajit M, Chantiratikul P. 2009. Flow Injection Analysis System for the Determination of Total Phenolic Compound by Using Folin-Ciocalteu Assay. Asian Journal of Applied Science 2, 184-190.

Li S, Tan HY, Wang N, Zhang ZJ, Lao L, Wong CW, Feng Y. 2015. The role of oxidative stress and antioxidants in liver diseases. International Journal of Molecular Science 16, 26087–26124.

Maroua K, Dalila B, Hanane EH, Mohammed L, Hassan B, Saad IK, Sadok B. 2017. Phytochemical screening, total phenolics and biological activities of Tunisian peganum harmala seed extracts. Journal of Chemical and Pharmaceutical Research 9(2), 32-39.

Mazandarani M, Sineh Sepehr K, Baradaran B, Khuri V. 2008. Autecology, phytochemical and antioxidant activity of Peganum harmala L. seed extract in North of Iran (Tash Mountains). Journal of Medicinal Plants and By-products 2, 151-156.

Shahverdi AR, Ostad SN, Khodaee S, Bitarafan L, Monsef-Esfahani HR, Jamalifar H, Mohseni M. 2008. Antimicrobial and cytotoxicity potential of Peganum harmala smoke. Pharmacognosy Magazine 4, 236-242.

Wang F, Li Y, Zhang YJ, Zhou Y, Li S, Li HB. 2016. Natural products for the prevention and treatment of hangover and alcohol use disorder. Molecules 21, 64-76.

Zhou Y,  Zheng J, Li S, Zhou T, Zhang P, Li HB. 2016. Alcoholic beverage consumption and chronic diseases. International Journal Environmental Research Public Health 13, 522-530.

Peganum harmala L., Methanol, Dichloromethane, Extraction, Total phenolic content, Antioxidant activity..
A comparative study of total phenolic contents and antioxidant potential of seeds of Peganum harmala.
Int. J. Biosci. 14(3), 431-437, March 2019.
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