International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Comparison of Zielh-Neelsen staining and polymerase chain reaction for the diagnosis of Johne’s disease in small ruminants in the district Lahore, Pakistan

By: Saba Badar, Ashiq Ali, Zafar Iqbal Choudary, Abdul Jabbar, Sidra Altaf, Qaisar Tanveer, Iqra Asif, Asima Tariq, Muhammad Saif Ur Rehman Babar, Muhammad Umar Amin, Muhammad Kashif, Akasha Tanveer, Kiran Choudhary, Sidra Aslam, Tehreem Umar

Key Words: Paratuberculosis, Small intestine, Zeihl-Nelson's staining, PCR.

Int. J. Biosci. 18(5), 7-13, May 2021.

DOI: http://dx.doi.org/10.12692/ijb/18.5.7-13

Certification: ijb 2021 0213 [Generate Certificate]

Abstract

Paratuberculosis is a bacterial disease that affects small and large ruminants and has an impact on the economy of the country in terms of production losses and cost of treatment and control. The objectives of this study were the diagnosis of Paratuberculosis or Johne’s disease (JD) in goats of Lahore, Pakistan and a comparison of the two diagnostic techniques for Paratuberculosis. Part of the small intestines and associated mesenteric lymph nodes were collected randomly from goats of all ages and both sexes. Those samples were subjected to the Herrold’s Egg Yolk Medium (HEYM) culture and DNA extraction followed by PCR and Ziehl-Neelsen’s staining. Acid-fast bacilli were found in the tissues that showed the gross lesions of severe enteritis and lymphadenitis in the collected samples. Results showed that there was a clinical and subclinical infection. This study showed PCR more sensitive in the detection of Insertion Sequence (IS) 900 genomic fragment as compared to the HEYM culture.

| Views 74 |

| Views 74 |

Comparison of Zielh-Neelsen staining and polymerase chain reaction for the diagnosis of Johne’s disease in small ruminants in the district Lahore, Pakistan

Acharya KR, Dhand NK, Whittington RJ, Plain KM. 2017. PCR Inhibition of a quantitative PCR for detection of Mycobacterium avium subspecies paratuberculosis DNA in feces: Diagnostic implications and potential solutions. Frontiers in microbiology 8, 115.

https://doi.org/10.3389/fmicb.2017.00115

Begg D, Whittington R. 2020. 12 Paratuberculosis in Sheep. Paratuberculosis: Organism, Disease, Control. 160.

Busatto C, Vianna JS, da Silva Junior LV, Ramis IB, da Silva PEA. 2019.  Mycobacterium avium: an overview. Tuberculosis 114, 127-134.

https://doi.org/10.1016/j.tube.2018.12.004

Eslami M, Shafiei M, Ghasemian A, Valizadeh S, AlMarzoqi AH, Shokouhi Mostafavi SK, Nojoomi F, Mirforughi SA. 2019. Mycobacterium avium paratuberculosis and Mycobacterium avium complex and related subspecies as causative agents of zoonotic and occupational diseases. Journal of Cellular Physiology 234(8), 12415-12421.

https://doi.org/10.1002/jcp.28076

Grant IR, Rowe MT, Dundee L, Hitchings E. 2001. Mycobacterium avium ssp. paratuberculosis: its incidence, heat resistance and detection in milk and dairy products. International Journal of Dairy Technology 54(1), 2-13

https://doi.org/10.1046/j.1471-0307.2001.00009.x

Green EP, Tizard MLV, Moss MT, Thompson J, Winterbourne DJ, McFadden JJ, Hermon-Taylor J. 1989. Sequence and characteristics of IS900, an insertion element identified in human Crohn’s disease isolate of Mycobacterium paratuberculosis. Nucl. Acids Res. 17 p 9063-9073.

https://doi.org/10.1093/nar/17.22.9063

Karuppusamy S, Kirby GM, Mutharia L, Tripathi BN. 2019. An update on Mycobacterium avium subspecies paratuberculosis antigens and their role in the diagnosis of Johne’s disease. World Journal of Microbiology and Biotechnology 35(8), 120.

https://doi.org/10.1007/s11274-019-2691-0

Li L,  Bannantine JP, Campo JJ, Randall A, Grohn YT, Katani R, Schilling M, Radzio-Basu J, Kapur V. 2017. Identification of sero-reactive antigens for the early diagnosis of Johne’s disease in cattle. PloS one. 12(9), e0184373.

https://doi.org/10.1371/journal.pone.0184373

Meijer ACE. 2019. Prevalence of hepatic epithelioid macrophage microgranulomas due to Mycobacterium avium subspecies paratuberculosis in Lambs on the North Island of New Zealand (Master’s thesis).

Morales-Pablos MI, Mejía-Sánchez P, Díaz-Aparicio E, Palomares-Resendiz EG, Gutiérrez-Hernández JL, Reyna-Granados JR, Luna-Nevárez P, Munguía-Xóchihua JA, Segura-Correa JC, Leyva-Corona JC. 2020. Risk factors associated with the seroprevalence of paratuberculosis in sheep flocks in the hot-arid region of Sonora, México. Tropical Animal Health and Production 52(3), 1357-1363.

https://doi.org/10.1007/s11250-019-02139-y

Okuni JB, Hansen S, Eltom KH, Eltayeb E, Amanzada A, Omega JA, Czerny CP, Abd El Wahed A, Ojok L. 2020. Paratuberculosis: A Potential Zoonosis and a Neglected Disease in Africa. Microorganisms 8(7), 1007.

https://doi.org/10.3390/microorganisms8071007

Sachse K, Frey J. 2003. PCR Detection of Microbial Pathogens. Humana Press, Totowa, New Jersey. 289-297.

Shah MM, Mistry M, Marsh SJ, Brown DA. Delmas P. 2002. Molecular correlates of the M‐current in cultured rat hippocampal neurons. The Journal of physiology 544(1), 29-37.

Sivakumar P, Tripathi BN, Singh N. 2005. Detection of Mycobacterium avium subsp. paratuberculosis in intestinal and lymph node tissues of water buffaloes (Bubalus bubalis) by PCR and bacterial culture. Veterinary Microbiology 108(3-4), 263-270. https://doi.org/10.1016/j.vetmic.2005.04.002

Soumya MP, Pillai RM, Antony PX, Mukhopadhyay HK, Rao VN. 2009. Comparison of faecal culture and IS900 PCR assay for the detection of Mycobacterium avium subsp. paratuberculosis in bovine faecal samples. Veterinary research communications 33(7), 781.

https://doi.org/10.1007/s11259-009-9226-3

Truyers I, Jennings A. 2016. Management and control of Johne’s disease in beef suckler herds. In Practice 38(7), 347-354.

http://dx.doi.org/10.1136/inp.i3394

Zarei M, Ghorbanpour M, Tajbakhsh S, Mosavari N. 2017. Comparison of ELISA method, PCR and Ziehl-Neelsen staining of the rectal mucosa for detection of Mycobacterium avium subsp. paratuberculosis infection in cattle 2(13), 29-37.

Saba Badar, Ashiq Ali, Zafar Iqbal Choudary, Abdul Jabbar, Sidra Altaf, Qaisar Tanveer, Iqra Asif, Asima Tariq, Muhammad Saif Ur Rehman Babar, Muhammad Umar Amin, Muhammad Kashif, Akasha Tanveer, Kiran Choudhary, Sidra Aslam, Tehreem Umar.
Comparison of Zielh-Neelsen staining and polymerase chain reaction for the diagnosis of Johne’s disease in small ruminants in the district Lahore, Pakistan.
Int. J. Biosci. 18(5), 7-13, May 2021.
https://innspub.net/ijb/comparison-of-zielh-neelsen-staining-and-polymerase-chain-reaction-for-the-diagnosis-of-johnes-disease-in-small-ruminants-in-the-district-lahore-pakistan/
Copyright © 2021
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update