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Cultural, morphological, pathogenic and molecular characterization of Mucor fragilis causing bunch rot of grapes in Pakistan and its bio management through plant essential oil

By: Salman Ghuffar, Gulshan Irshad, Farah Naz, Muhammad Azam Khan

Key Words: Grapes, Pakistan, Plant essential oil, Mucor fragilis, Bunch rot

Int. J. Biosci. 12(4), 392-399, April 2018.

DOI: http://dx.doi.org/10.12692/ijb/12.4.392-399

Certification: ijb 2018 0026 [Generate Certificate]

Abstract

Post-harvest disease of grape (Vitis vinifera) especially rot caused by Mucor sp. are responsible to minimize the market value of grapes. Twenty two isolates recovered from five different locations of fruit markets located in Attock district of Punjab province, Pakistsn. During morphological characterization light sulphur yellow to off white, fluffy, fast-growing colonies were observed. Width of sporangiophores ranged 6.5 to 10.5 μm; sporangia were globose to sub globose, containing yellow to orange sporangiospores. For molecular characterization, the internal transcribed spacer (ITS1, 5.8s and ITS2) regions of two pathogenic isolates were amplified using ITS1 & ITS4 primers. Sequence comparison revealed 98-100% genetic homology (Accession no.KX550076 and KY290546) with previously reported isolates of M. fragilis. Among, In vitro evaluation of five plant essential oils against M. fragilis. Thyme oil at 0.01% concentration showed the minimum mycelia radial growth (3.6cm) 3 days after incubation at 25±2°C as compared to control (8.0cm). The thyme (Eo) was further evaluated for the presence of antimicrobial compounds viz. terpenoids, alkaloids, phenolics and saponins employing standard protocols and found positive for the presence of all compounds. During the application of thyme oil at (0.1%) concentration on fruit bunches for the determination of decaying percentage. The result showed 9.53% decay caused by M. fragilis on treated bunches up to six days of storage and control was 71.34% calculated. Keeping in all view, Thyme oil possessing good inhibitory action upon M. Fragilis may be a potential candidate for preservation and extension of shelf-life of grapes commercially.

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Cultural, morphological, pathogenic and molecular characterization of Mucor fragilis causing bunch rot of grapes in Pakistan and its bio management through plant essential oil

Abd-Alla MA, Nadia GE, Eman RH. 2013. Effect of Some Natural Plant Extracts & Plant Essential Oils on Suppressive of Penecillium digitatium (Pers.:Fr.) Sacc. and its enzyme activity which caused Citrus Green Mold for Navel Oranges in Egypt. Journal of Applied Sciences Research 9(6), 4073-4080.

Agarwal C, Singh RP, Agarwal R. 2002. Grape seed extract induces apoptotic death of human prostate carcinoma DU145 cells via caspases activation accompanied by dissipation of mitochondrial membrane potential and cytochrome release. Carcinogenesis 23(11), 1869-1876.

Akhtar N, Anjum T, Jabeen R. 2013. Isolation and identification of storage fungi from citrus sampled from major growing areas of Punjab, Pakistan. International Journal of Agriculture and Biology 15, 1283-1288.

Anonymous. 1985. SAS introductory guide. 3rd ED. SAS Institute, Cary, NC.

Behr M, Legay S, Evers D. 2013. Molecular identification of botrytis cinerea, penicillium spp. and cladosporium spp. in luxembourg. Journal International des Sciences de la Vigne et du Vin 47(3), 239-247.

Ellis MB. 1971. Dematiacious hyphomycetes. CABI, Kew, England, 608.

Evans WC. 1997. Trease and Evans phamacognosy. (14th ED) Harcourt Brace and Company. Asia Pvt. Ltd. Singapore 343.

FAO. 2013. Food and Agriculture Organization. Area and production of grapes. http://www. faostat. fao.org.

Gatto MA, Ippolito A, Linsalata V, Cascarano NA, Nigro F, Va­nadia S, Venere D. 2011. Activity of extracts from wild edible herbs against postharvest fungal diseases of fruit and vegetables. Postharvest Biology and Technology 61, 72-82.

Ghaouth AE, Arul J, Grenier J, Asselin A. 1992. Antifungal activity of chitosan on two post harvest pathogens of strawberry fruits. Phytopathology       82, 398-402.

Ghuffar S, Irshad G, Naz F, Rosli HB, Hyder S, Mehmood N, Zeshan MA, Raza MM, Mayer CG, Gleason ML. 2017. First report of two Penicillium spp. causing post-harvest fruit rot of grapes in Pakistan. Plant Disease (In Press) https://doi.org/10.1094/PDIS-10-17-1616-PDN

Ghuffar S, Irshad G, Naz F, Zhang X, Bashir A, Yang H, Zhai F, Gleason ML. 2017. First report of post-harvest rot caused by Pestalotiopsis sp. on grapes in Punjab, Pakistan (In press) https://doi.org/10.1094/PDIS-08-17-1281-PDN

Ghuffar S, Irshad G, Shahid M, Naz F, Riaz A, Khan MA, Mehmood N, Sattar A, Asadullah HM, Gleason ML. 2018. First report of Alternaria alternata causing fruit rot of grapes in Pakistan. Plant Disease (In press) https://doi.org/10.1094/PDIS-01-18-0096-PDN

Hall TA. 1999. Bio Edit: a user friendly biological sequence alignment editor and analysis program for windows 95/98/ NT. Nucleic Acids Symposium Series no 41, 95-98.

Javed S, Javaid A, Anwar W, Majeed RA, Akhtar R, Naqvi SF. 2017. First Report of Botrytis Bunch Rot of Grapes Caused by Botrytis cinerea in Pakistan. Plant Disease 101(6), 1036.

Kassemeyer HH. 2009. Fungi of Grapes, Biology of Microorganisms on Grapes, in Must and in Wine      4, 61-89.

Khan AD, Nabi G. 2010. Grapes cultivation in Charsadda a district of cmp II in Baluchistan. www.npfs-minfa.gov.pk.

Kokate CK. 1999. Practical Pharmacognasy. (4th ED) Vallabh Parkashan Publications, New Dehli, India 115.

Kumar S, Stecher G, Tamura K. 2016. MEGA7: molecular evolutionarygenetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33, 1870-1874.

Lu JH, He YQ. 2010. Fumigant toxicity of Ailanthus altissima Swingle, Atractylodes lancea (Thunb.) DC. and Elsholtzia stauntonii Benth extracts on three major stored-grain insects. Industrial Crops and Products 32(3), 681-683.

Naz F, Qamarunnisa S, Shinwari ZK, Azhar A, Ali SI. 2013. photochemical investigations of Tamarix indica and Tamarix passernioides Del.ex Desv. Leaves from Pakistan. Pakistan journal of Botany 45(5), 1503-1507.

Reverchon E. 1997. Supercritical fluid extraction and fractionation of essential oils and related products. Journal of Supercritical Fluids 10, 1-37.

Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. 1997. The Clustal-X windows interface: flexible strategiesfor multiple sequence alignment aided by quality analysistools. Nucleic Acids Research 25, 4876-4882.

Thuong T, Nguyen T,  Duong TT, Lee HB. 2016. Characterization of Two New Records of Mucoralean Species Isolated from Gut of Soldier Fly Larva in Korea. Mycobiology 44(4), 310-313.

White TJ. 1990. PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego 315.

Zoffoli JP, Latorre BA, Naranjo P. 2007. Hairline, a postharvest cracking disorder in table grapes induced by sulfure dioxide. Postharvest Biology and Technology 47- 90.

Salman Ghuffar, Gulshan Irshad, Farah Naz, Muhammad Azam Khan.
Cultural, morphological, pathogenic and molecular characterization of Mucor fragilis causing bunch rot of grapes in Pakistan and its bio management through plant essential oil.
Int. J. Biosci. 12(4), 392-399, April 2018.
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