International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Detection of bovine tuberculosis in cow carcasses in Baghdad

By: Maysoon S. Abbas, Amina N. AL-Thwaini, Ayed M. AL-Dilimi

Key Words: Tuberculosis, Bovine, Mycobacteria, Carcasses, Lymph node.

Int. J. Biosci. 15(2), 348-354, August 2019.

DOI: http://dx.doi.org/10.12692/ijb/15.2.348-354

Certification: ijb 2019 0095 [Generate Certificate]

Abstract

Bovine Tuberculosis is a contagious disease ,both animals and humans are highly susceptible to infection, infected animal act as source for infected farmers, veterinarians, slaughterhouse workers, butchers   so the importance of our study is to stand on the extent of tuberculosis infection in cow carcasses in three abattoris of Baghdad (Al-shula,Al-karkh and Al-dura.), Thousands carcasses were examined, six hundreds and fourty two samples of lymph nodes and organs were collected from (128) carcasses these involved (10) retropharyngeal lymphnode, (8) portal lymph nodes,(110)mesenteric lymph node(85)spleen samples, (4) supramammary lymph node, and (4)uterus samples, the samples were transported to laboratory for bacteriological culturing The were processed for isolation of mycobacteria following standard procedures for homogenization, suspension, centrifugation and decontamination with 4 % of NaOH and were inoculated on Lowenstein-Jensen (L-J) media with and without pyruvate and incubated at 37ºC incubator for culture for a maximum up to 8 wk. after growth appear direct microscopic examination  and biochemical test for mycobacteria (growth on mac-conckey without crystal violet ,urease test, catalas test,nitrate reduction,(thiophen2 carboxylic acid hydroxide(TCH),sensitivity to antimycobacterial drug ethambutol (EMB), Isonicotinic hydrazide (INH), Streptomycin(SM), Rimfampicin (RMP), Para-aminosalicylic acid(PAS). the results indicated that the percentage of infection( 2.8% and0.6%)in Al-shula and Al-karkh abattoirs respectively while AL-dura abattoir was found (0%)and  (8.1%) of lymphnode ,(17.6%)of lung,(3.8%)of livers,(3.5%)of spleen and (25%) for each supramammary lymph node and uterus infected with bovine tuberculosis twenty bacterial isolated belonged to mycobacterium were isolated from carcasses,(17) of them were Mycobacterium bovis.this is the first study in  isolation of Mycobacterium bovis from cow carcasses in Iraq.

| Views 25 |

Detection of bovine tuberculosis in cow carcasses in Baghdad

Abdul Basit, Mubbashir Hussain, Sultan Ayaz, Muhammad Shahid, Kashif Rahim, Iqbal Ahmad, Riaz Ullah, Abeer Hashem, Elsayed Abd-Allah, Abdulaziz Abdullah Alqarawi, Naila. 2015. Isolation and identification of Mycobacterium bovis and Mycobacterium tuberculosis from animal tissues by conventional and molecular method Indian Journal of animal research. 49(5), 687-693.

AL-Azwi AM, AL-Darriaji AM, AL-Joboury KH. 1992. Apathological study on bovine tuberculosis in baghdad .Journal of Veterinary research 2(1), 102-106.

Brown WH, Hernandez De.  Anda J. 1998.Tuberculosis in adult beef cattle of Mexican origin shipped direct to slaughterin to Texas .Journal American Veterinary Medical Association 15(14), 557-9.

Clarice Q. Fujimura Leite, Ivone S. Anno, Sergio R. de Andrade Leite, Eliana Roxo, Glenn P. Morlock, Robert C. Cooksey Faculdade de Ciências. 2003. Isolation and Identification of Mycobacteria from Livestock Specimens and Milk Obtained in Brazil, Ministério da Instituto Oswaldo Cruz Saúde     98(3), 319-323,

Claxton PD, Eamens GJ, Mylreay PJ. 1979. Laboratory diagnosis of bovine tuberculosis Australian Veterinary Journal 55, 514-519.

Diana L, Whipple Carole A, Bolin Janice M, Miller. 1996. Distribution of lesions in cattle infected with Mycobacterium bovis Journal Veterinary Diagnostic Investigation 8, 351 -354.

Francis J. 1971.Susceptibility to tuberculosis and the route of infection.Australian Veterinary Journal 47, 414.

Joseph KN, Kuria 2019. Diseases Caused by Bacteria in Cattle: Tuberculosis [Online First], Intech Open.

http://dx.doi.org/10.5772/intechopen.82051.

https://www.intechopen.com/online-first/diseases-caused-by-bacteria-in-cattle-tuberculosis

Henandez De Anda J, Renteria Evangelisa J, Lopezalencia C. 1997. An abattoir monitoring system for diagnosis of tuberculosis in cattle in Baja.California, Mexico, Journal American Veterinary Medical Association 2(11), 709-711.

Hernandez J, Basca D. 1998. Effect of tuberculosis on milk product on in dairy cow. Journal. American Veterinary Medical Association 213(6), 851-854.

Hein WR, Tomosovic AA. 1981. An abattoir survey of tuberculosis inferal buffaloes Australian Veterinary Journal 57, 543-547.

Kustandi JD. 1984. Isolation and identification of mycobacterium from cattle in and around Baghdad .MSC. Thesis, College of Veterinary medicine .University of Baghdad p 34.

Luna HT, Lee. 1968. Manual of histological staining methods of the armed forces. Institute of pathology 3rd ed. The blakiston division McGraw-Hill book Co. New York Toronto, London, Sydney.

Menzies Neill SD. 2000. Cattle-to-cattle transmission of bovine tuberculosis. Veterinary Journal 160, 92-106.

Mamadou Diallo, Bassirou Diarra, Moumine SanogoAntieme C.G. Togo, Anou M.  Somboro Mariam H. Diallo, Brehima Traore, Mamoudou MaigaYounoussa KonéKarim TounkaraYeya dit Sadio SarroBocar Baya, Drissa Goita, Hamadoun Kassambara, Bindongo P. P. Dembélé, Sophia Siddiqui, Robert L. MurphySounkalo Dao, Souleymane DialloAnatole TounkaraMamadou Niang. 2016. Molecular identification of Mycobacterium bovis from cattle and human host in Mali: expanded genetic diversity BMC Veterinary research  12, 145

 Nolwazi L. Bhembea B, Ishmael F. Jajac, Uchechukwu U. Nwodoa B, Anthony I. Okoha B, Ezekiel. 2017. Prevalence of tuberculous lymphadenitis in slaughtered cattle in Eastern Cape, South Africa International Journal of Infectious Diseases 61, 27-37.

O’Reilly L, Mand Daborn CJ. 1995. The epidemiology of Mycobacterium bovis infections in animals and man: a review Tubercle and Lung Disease  76(1), 1-46.

Okolo MI. 1992. Tuberculosis in apparently healthy milch cows. Microbiology 69, 105-111.

Palmer W, Ray Waters DL, Whipple. 2002. Aerosol delivery of virulent Mycobacterium bovis to cattle Tuberculosis 82(6), 275—282.

Roberts CD, Koneman EW Kim YK. 1991. Mycobacterium p. (304-339). In manual of clinical microbiology5th ed.Balows A. (penin chiel) American Society for microbiology. Walingtion D.C.V.S.A.(cited from Qunin, et al.,1994).

Sa’idu AS, EC, Dzikwi AA, Kwaga JKP,  Gamawa AA, Sman AU, Maigari SA,  Ibrahim S. 2015. Detection of Mycobacterium bovis in Organs of Slaughtered Cattle by DNA-Based Polymerase Chain Reaction and Ziehl-Neelsen Techniques in Bauchi State, Nigeria Journal of Veterinary Medicine Volume, Article ID 921868, 7 pages.

Srivastava K, Chauhan DS, Gupta P, Singh HB, Sharma VD, Yadav VS, Sreekumaran Thakral SS, Dharamdheeran JS, Nigam P, Prasad HK Katoch VM. 2008. Isolation of Mycobacterium bovis & M. tuberculosis from cattle of some farms in north India – Possible relevance in human health Indian Journal of medical research. 128, 26-31.

Maysoon S. Abbas, Amina N. AL-Thwaini, Ayed M. AL-Dilimi.
Detection of bovine tuberculosis in cow carcasses in Baghdad.
Int. J. Biosci. 15(2), 348-354, August 2019.
https://innspub.net/ijb/detection-bovine-tuberculosis-cow-carcasses-baghdad/
Copyright © 2019
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update