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Detection of Escherichia coli O157 H7 isolated from infected dogs with urinary tract infections using real time – polymerase chain reaction

By: Aida H. Ibrahim, Ban N.Nadhom, Ansam K. Mohammed

Key Words: E. coli, E. coli O157 H7, 16srRNA genes, Dogs, Urinary tract infection, RT-PCR.

Int. J. Biosci. 13(4), 102-110, October 2018.

DOI: http://dx.doi.org/10.12692/ijb/13.4.102-110

Certification: ijb 2018 0014 [Generate Certificate]

Abstract

This research was approved to isolate, detects, diagnosis and molecular composition of Escherichia coli O157 H7 from UTI in Baghdad city. 67 urine samples were collected from the referring dogs into the Adan Teaching Veterinary Hospitals from the period of October 2017 to June 2018. The urine samples were collected by using urethral catheterization method. The samples were cultured, the recovered colonies were diagnosed microscopically (Gram stain), morphorgically and biochemically according to slandered methods. The results showed that the frequency of isolated bacterial spp. were 26 (38.8%) of 67 samples of urine, sex and age disseminations of dogs with bacteriuria .The bacteria were insulated in clean culture from 20 (76.92%) samples and were insulated in diverse culture from 6 (23.07%) samples. A total of 37 strains were isolated from urine samples. Of the strains isolated, 12(32.43%) were as Escherichia coli, 7(18.91%) as Streptococcus species, 6 (16.21%) as Staphylococcus species, 4 (10.81%) as Klebsiella species. 3(8.10%) as Proteus species. and Pseudomonas aeruginosa and 2 (5.40%) as Corynebacterium species. Specific forward and reverse primers were designed according to a program from NCBI-Genbank for Escherichia coli and E. coli O157 H7 To study sequence of these genes  after amplification of 16srRNA genes. Genetic identities results showed that E. coli and E. coli  O157 H7 isolates gave 100%  matching and resembling the reference strains.

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Detection of Escherichia coli O157 H7 isolated from infected dogs with urinary tract infections using real time – polymerase chain reaction

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Aida H. Ibrahim, Ban N.Nadhom, Ansam K. Mohammed.
Detection of Escherichia coli O157 H7 isolated from infected dogs with urinary tract infections using real time – polymerase chain reaction.
Int. J. Biosci. 13(4), 102-110, October 2018.
https://innspub.net/ijb/detection-escherichia-coli-o157-h7-isolated-infected-dogs-urinary-tract-infections-using-real-time-polymerase-chain-reaction/
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