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Detection of multi-drug resistant Streptococcus pyogenes from raw milk samples in Faisalabad

By: Muhammad Fahad Chaudhary, Ali Abbas Dilawar, Muhammad Asim, Suneel Ahmad, Unsa Saleem

Key Words: MDR, Raw milk, Streptococcus, MIC, Antibiotic susceptibility.

Int. J. Biosci. 17(6), 353-359, December 2020.

DOI: http://dx.doi.org/10.12692/ijb/17.6.353-359

Certification: ijb 2020 0114 [Generate Certificate]

Abstract

Milk is a very important source of nutrition for humans as well as animals. It contains carbohydrates, fats, vitamins, and minerals. Due to its nutritious composition, it is an excellent medium for the growth of microorganisms. Milk gets contaminated by different microorganisms including Streptococcus species.  Considering its importance, the present study was designed to check the microbial contamination of raw milk. 90 samples were collected from different areas of Faisalabad city. Mainly Streptococcus species were targeted and isolated. Out of 90, only 23 samples were found positive for Streptococcus species. Most species were identified as Streptococcus pyogenes by biochemical characteristics. Antibiotic susceptibility testing was performed and isolates were found to be susceptible to Amikacin (30µg), Tazobactam (110µg), Levofloxacin (5µg), Tobramycin (30µg), Ampicilin-sulbactam (20µg), Chloromphenicol (30µg), and Gentamycin (30µg) and were resistant to Cefoxitin (30µg), Tigecycline (15µg), Cefipime (30µ), Ampicilin (10µ), Cefotoxime (10µg), Trimethoprim (25µg), Cefuroxime (30µg), Cefixime (5µg), Tetracycline (10µg). For further confirmation, Minimum Inhibitory Concentration was performed according to CLSI guidelines and results showed that isolates were susceptible to only Ampicillin, Amikacin and Erythromycin. Animals suffering from clinical or sub-clinical mastitis are the source of Streptococcal contamination of milk. The current study supports the finding that raw milk can be regarded as a critical source of MDR bacteria. Strict monitoring and the implementation of effective hygienic should be implemented.

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Detection of multi-drug resistant Streptococcus pyogenes from raw milk samples in Faisalabad

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Muhammad Fahad Chaudhary, Ali Abbas Dilawar, Muhammad Asim, Suneel Ahmad, Unsa Saleem.
Detection of multi-drug resistant Streptococcus pyogenes from raw milk samples in Faisalabad.
Int. J. Biosci. 17(6), 353-359, December 2020.
https://innspub.net/ijb/detection-multi-drug-resistant-streptococcus-pyogenes-raw-milk-samples-faisalabad/
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