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Determination of the novel SNPs in UQCC gene among three different cattle breeds in Pakistan

By: Majida Parveen Narejo, Javed Ahmed Ujan, Shahnawaz Ujan, Sham Lal, Om Parkash, Munir Ahmad, Majeeda Ruk, Fawad Shabir Memon, Aziz Ahmed Ujjan, Shahid Jakhrani

Key Words: Cattle, DNA sequencing, PCR, SNPS, UQCC

Int. J. Biosci. 18(5), 167-173, May 2021.

DOI: http://dx.doi.org/10.12692/ijb/18.5.167-173

Certification: ijb 2021 0237 [Generate Certificate]

Abstract

For farm animals, the quality of meat is of great economic importance. It is influenced by the environment and by the multi-genes. In recent decades, developments for molecular genetics have assisted in the discovery of genes or markers associated with traits that affect the quality of meat. The aim of this study was to investigate the novel SNPs in UQCC gene that may affect the meat quality. Initially, UQCC gene were amplified from cattle DNA samples and visualized on gel electrophoresis. Later, these PCR products were sent for DNA sequencing followed by SNP analysis. Study showed that five mutations were spotted in Exon 1 region of UQCC gene in all three cattle breeds. Based on genetic code, three different types of mutation were observed, namely, silent, missense and delete mutations. Silent mutation was common between Dhanni and Red Sindhi cattle breeds. Missense mutation was observed only in Red Sindhi cattle breed whereas delete mutation was very common among all three cattle breeds. Missense mutation observed in Red Sindhi cattle may be consider as a good sign and can produce good impact on meat traits as it codes for essential amino acids. These results suggest that Red Sindhi cattle breed can be implied for breed admixture and marker assisted selection in cattle breeding.

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Determination of the novel SNPs in UQCC gene among three different cattle breeds in Pakistan

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Majida Parveen Narejo, Javed Ahmed Ujan, Shahnawaz Ujan, Sham Lal, Om Parkash, Munir Ahmad, Majeeda Ruk, Fawad Shabir Memon, Aziz Ahmed Ujjan, Shahid Jakhrani.
Determination of the novel SNPs in UQCC gene among three different cattle breeds in Pakistan.
Int. J. Biosci. 18(5), 167-173, May 2021.
https://innspub.net/ijb/determination-of-the-novel-snps-in-uqcc-gene-among-three-different-cattle-breeds-in-pakistan/
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