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Diversity of Diptera in Bohol Island, Philippines, using DNA barcodes

By: Reizl Jose, Sharon Rose Tabugo, Olga Nuñeza, Rudolf Meier

Key Words: Diptera, Conservation, Molecular Operational Taxonomic Unit (MOTU), Microalgae, Malaise trap.

Int. J. Biosci. 14(6), 122-131, June 2019.


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Studying island systems offers a great advantage to look into diversity of organisms because this provides opportunity for both dispersal, isolation mechanisms and key to conservation. The study was conducted to determine the diversity of true flies in Bohol, Island, Philippines. Malaise traps were used to collect samples from different habitats. A total of 94 Molecular Operational Taxonomic Units (MOTUs) clustering at 3% threshold were identified. The result showed a diversity index of H’=3.70. Observed diversity may be influenced by availability of heterogeneous habitats present between sampling sites and longer period of sampling. Sixty-nine (69) presumptive Diptera species belonging to eight (8) families were identified in Bohol. In addition, family Mycetophilidae showed the highest number of species in Bohol at 49.28% of the total species. Moreover, DNA barcodes showed presumptive species belonging to eight families of Diptera were found only in Magsaysay Park, Bohol. These include: Culicidae, Dolichopodidae, Empididae, Mycetophilidae, Sepsidae, Stratiomyidae, Tabanidae, and Tephritidae. This is possibly due to the unique characteristic of habitat in Magsaysay Park, Bohol, hence, a key site for conservation.

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Diversity of Diptera in Bohol Island, Philippines, using DNA barcodes

Ang Y, Lim GS, Meier R. 2008. Morphology and DNA sequences confirm the first Neotropical record for the Holarctic sepsid species Themira leachi (Meigen)(Diptera: Sepsidae). Zootaxa 1933, 63-65.

Ang Y, Puniamoorthy J, Pont AC, Bartak M, Blanckenhorn WU, Eberhard WG, Meier R. 2013. A plea for digital reference collections and other science-based digitization initiatives in taxonomy: Sepsidnet as exemplar. Systematic Entomology 38, 637-644.

Ashfaq M, Akhtar S, Rafi MA, Mansoor S, Hebert PD. 2017. Mapping global biodiversity connections with DNA barcodes: Lepidoptera of Pakistan. PloS one 12, e0174749.

Bickel DJ. 2013. The family Dolichopodidae (Diptera) of the Pilbara region, Western Australia in its Australasian biogeographic context, with the description of 19 new species. Records of the Western Australian Museum 83, 291-348.

Bickford D, Lohman DJ, Sodhi NS, Ng PK, Meier R, Winker K., Ngram KK, Das I. 2007. Cryptic species as a window on diversity and conservation. Trends in ecology & evolution 22, 148-155.

Borkent A. 1981. The distribution and habitat preferences of the Chaoboridae (Culicomorpha: Diptera) of the Holarctic region. Canadian Journal of Zoology 59, 122-133.

Didham RK, Ghazoul J, Stork NE, Davis AJ. 1996. Insects in fragmented forests: a functional approach. Trends in Ecology & Evolution 11, 255-260.

Ella DS. 2017. PEAKWARE: World Mountain Encyclopedia. Date accessed: April 27.

Esselstyn JA, Oliveros CH, Moyle RG, Peterson AT, McGuire JA, Brown RM. 2010. Integrating phylo- genetic and taxonomic evidence illuminates complex biogeographic patterns along Huxley’s modifica- tion of Wallace’s Line. Journal of Biogeography 37, 2054–2066.

Greve C, Gimnich F, Hutterer R, Misof B, Haase M. 2012. Radiating on oceanic islands: patterns and processes of speciation in the land snail genus Theba (Risso 1826). PLoS One, 7e34339.

Guernaoui S, Boumezzough A. 2009. Habitat preferences of phlebotomine sand flies (Diptera: Psychodidae) in southwestern Morocco. Journal of medical entomology 46, 1187-1194.

Hajibabaei M, McKenna C. 2012. DNA mini-barcodes. In DNA barcodes. Humana Press, Totowa, NJ. p 339-353.

Hall R. 1998. The plate tectonics of Cenozoic SE Asia and the distribution of land and sea. Pp. 99–132 in R. Hall and J. D. Holloway, eds., Biogeography and geological evolution of SE Asia. Backhuys Publishers, Leiden, p 417.

Hebert PD, Cywinska A, Ball SL, Dewaard JR. 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences 270, 313-321.

Inclan DJ, Stireman III JO. 2011. Tachinid (Diptera: Tachinidae) parasitoid diversity and temporal abundance at a single site in the northeastern United States. Annals of the Entomological Society of America 104, 287-296.

Ivković M, Miliša M, Baranov V, Mihaljević Z. 2015. Environmental drivers of biotic traits and phenology patterns of Diptera assemblages in karst springs: The role of canopy uncovered. Limnologica 54, 44-57.

Jakovlev J, Kjærandsen J, Viklund B. 2008. Fungus gnats (Diptera: Bolitophilidae, Diadocidiidae, Ditomyiidae, Keroplatidae & Mycetophilidae) from Tyresta National Park and Nature Reserve in Sweden. Sahlbergia 14, 29-52.

Katoh K, Standley DM. 2013. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular biology and evolution 30, 772-780.

Lee T, Ming T. 2016. Next-Gen barcoding reveals overwhelming species diversity in two insect groups: Implications for morphology-based taxonomy (Thesis). Department of Biological Sciences National University of Singapore.

Meier R, Zhang G, Ali F. 2008. The use of mean instead of smallest interspecific distances exaggerates  the size of the “barcoding gap” and leads to misidentification. Systematic biology 57, 809-813.

Meier R, Wong W, Srivathsan A, Foo M. 2016. $1 DNA barcodes for reconstructing complex phenomes and finding rare species in specimen‐rich samples. Cladistics 32, 100-110.

Novotny V, Drozd P, Miller SE, Kulfan M, Janda M, Basset Y, Weiblen GD. 2006. Why are there so many species of herbivorous insects in tropical rainforests? Science 313, 1115-1118.

Økland B. 1994. Mycetophilidae (Diptera), an insect group vulnerable to forestry practices? A comparison of clearcut, managed and semi-natural spruce forests in southern Norway. Biodiversity & Conservation 3, 68-85.

Oosterbroek P. 1998. The families of Diptera of the Malay Archipelago 1, Brill.

Ricklefs RE, Bermingham E. 2007. The causes of evolutionary radiations in archipelagoes: Passerine birds in the Lesser Antilles. The American Naturalist 169, 285–297.

Ryberg M. 2015. Molecular operational taxonomic units as approximations of species in the light of evolutionary models and empirical data from Fungi. Molecular ecology 24, 5770-5777.

SánchezGonzález LA, NavarroSigüenza AG, Krabbe NK, Fjeldså J, GarcíaMoreno J. 2015. Diversification in the Andes: the Atlapetes brush‐finches. Zoologica Scripta 44, 135-152.

Scotland RW, Olmstead RG, Bennett JR. 2003. Phylogeny reconstruction: the role of morphology. Systematic Biology 52, 539-548.

Siefert A, Green T. 2005. Effects of changes in canopy cover on understory vegetation in the Long Island Pine Barrens. In Brookhaven (NY): Pine Barrens Research Forum.

Srivathsan A, Meier R. 2012. On the inappropriate use of Kimura‐2‐parameter (K2P) divergences in the DNA‐barcoding literature. Cladistics 28, 190-194.

Stork NE. 1997. Measuring global biodiversity and its decline, pp. 41Ð 68. In M. L. Reaka-Kudla, D. E. Wilson, and E. O. Wilson [eds.], Biodiversity II: understanding and protecting our biological resources. Joseph Henry Press, Washington, DC.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular biology and evolution 30, 2725-2729.

Tan DS, Ang Y, Lim GS, Ismail MRB, Meier R. 2010. From ‘cryptic species’ to integrative taxonomy: an iterative process involving DNA sequences, morphology, and behaviour leads to the resurrection of Sepsis pyrrhosoma (Sepsidae: Diptera). Zoologica Scripta 39, 51-61.

Tews J, Brose U, Grimm V, Tielbörger K, Wichmann MC, Schwager M, Jeltsch F. 2004. Animal species diversity driven by habitat heterogeneity/diversity: the importance of keystone structures. Journal of biogeography 31, 79-92.

Thompson FC. [Ed.]. 2008. Biosystematic database of world Diptera. Accessed on 12 November 2016).

Villegas RN. ed. 2003. Tubod: the heart of Bohol. Manila: National Commission for Culture and the Arts. ISBN 9718140360.

Vogler AP, Monaghan MT. 2007. Recent advances in DNA taxonomy. Journal of Zoological Systematics and Evolutionary Research 45, 1-10.

Wong WH, Tay YC, Puniamoorthy J, Balke M, Cranston PS, Meier R. 2014. Direct PCR’optimization yields a rapid, cost‐effective, nondestructive and efficient method for obtaining DNA barcodes without DNA extraction. Molecular Ecology Resources 14, 1271-1280.

Zhang J, Kobert K, Flouri T, Stamatakis A. 2013. PEAR: a fast and accurate Illumina Paired-End reAd mergeR. Bioinformatics 30, 614-620.

Reizl Jose, Sharon Rose Tabugo, Olga Nuñeza, Rudolf Meier.
Diversity of Diptera in Bohol Island, Philippines, using DNA barcodes.
Int. J. Biosci. 14(6), 122-131, June 2019.
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