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Effect of Glomus intraradices in improving the tolerance of a hybrid family of Theobroma cacao L. against Phytophthora megakarya

By: Simo Claude, Fankem Henri, Njonzo-Nzo Stephanie Alvine, Tassong Saah Denis, Taffouo Victor Desire, Djocgoue Pierre François

Key Words: Theobroma cacao, Phytophthora megakarya, Glomus intraradices, Necrosis, Tolerance, Phenolic compounds.

Int. J. Biosci. 14(4), 76-94, April 2019.

DOI: http://dx.doi.org/10.12692/ijb/14.4.76-94

Certification: ijb 2019 0008 [Generate Certificate]

Abstract

The cocoa tree (Theobroma cacao L.) is a perennial plant of economic importance grown in several cocoa producing countries. Phytophthora megakarya is an oomycete that has a negative impact on cocoa production in Cameroon causing substantial yield losses (up to 100%). To remedy this, the use of genetic resources for the selection of highly tolerant genotypes is necessary for its production. The leaf necrosis of cacao in absence and in the presence of Glomus intraradices was measured, the accumulation of phenolic compounds was evaluated in the leaves of genotypes after Phytophthora megakarya infection in the absence and presence of Glomus intraradices. The analysis maps of the development of necrosis and the accumulation of phenolic compounds obtained on the basis of the necrotic area and the contents of phenolic compounds was used to appreciate the degree of tolerance of the hybrid genotypes againts Phytophthora megakarya. In addition, the development of necrosis on surfaces less than 0.5 cm2 in the presence of Glomus intraradices was observed in 75% against 94% (without control of G. intraradices) of hybrid genotypes. Glomus intraradices have significantly improved the tolerance of these hybrid genotypes. Moreover, it has been found that some hybrid genotypes of the Ficup offspring exhibiting better tolerance with necrotic surfaces between 0 and 0.5 cm2 in the presence of G. intraradices. Sensitive hybrid genotypes in the presence of G. intraradices, increased their degree of tolerance against the attack of Phytophthora megakarya. This G. intraradices can therefore be used by farmers in cocoa plantations to improve their yield.

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Effect of Glomus intraradices in improving the tolerance of a hybrid family of Theobroma cacao L. against Phytophthora megakarya

Ajeesh R, Vikas K, Santoshkumar A, Surendra K. 2015. Harnessing arbuscular mycorrhizal fungi (amf) for quality seedling production. Research Journal of Agricultural and Forestry ciences 3(6), 22-40.

Al-Askar AA, Rashad YM. 2010. Arbuscular mycorrhizal fungi: a biocontrol agent against common bean Fusarium root rot disease. Plant Pathology Journal 9(1), 31-38.

http://dx.doi.org/10.3923/ppj.2010.31.38.

Blaha G, Lotode R. 1976. Un critère primordial de la sélection du cacaoyer au Cameroun : la résistance à la pourriture brune des cabosses (P. palmivora).Café Cacao Thé 20, 97-115.

Bowers JH, Sanogo S, Tondje PR, Hebbar PK, Lumsden RD. 2001. Developing strategies for biological control of black pod, monilia pod rot, and witches’broom on cacao. In : Proceedings of the 1st West and Central Africa training workshop on biocontrol of plant diseases, with special reference to cacao black pod diseases. 25-29 June, 2001. Douala, Cameroon, p10-16.

Cilas C, Ndounbé-Nkeng M, Bidzanga N, N’goran J. 2004. Incidence de la maladie et de résistance sur le terrain. Dans: Cilas C. et Despréaux D. (eds). Amélioration de la résistance des arbres de cacao à la maladie de Phytophthora. Repères, CIRAD, Paris, France, p 171-193

Constabel CP, Lynn Y, Patton JJ, Christopher ME. 2000. Polyphenoloxidase from hybrid poplar. Cloning and expression in response to wounding and herbivory.  Plant Physiology 124, 285-295.

Coulibaly K, Kebe IB, KoffI NK, Mpik J, Kone D. 2013. Caractérisation des isolats de Phytophthora spp du verger cacaoyers de Côte d’Ivoire. Journal of Applied Biosciences 70, 5567– 5579.

Damodaran T, Kumar N, Kavino M. 2009. Breeding and evaluation of Musa hybrids resistant to Fusarium oxysporum f. sp. cubense race 1. Fruits 64, 3-12.

http://dx.doi.org/10.1051/fruits/2008044.

Djocgoue PF, Boudjeko T, Mbouobda HD, Nankeu DJ, El Hadrami I, Omokolo ND. 2007. Heritability of phenols in the resistance of Theobroma cacao against Phytophthora megakarya, the causal agent of black pod disease. Journal of Phytopathology 155, 519-525.

http://dx.doi.org/10.1111/j.1439-0434.2007.01268.x.

Djocgoue PF, Simo C, Mbouobda HD, Boudjeko T, Nankeu DJ, Omokolo ND. 2010. Assessment and heritability of productivity and tolerance level to Phytophthora megakarya in two hybrid population of Theobroma cacao. Journal of Plant Pathology 92 (3), 607-617.

Effa PO, Jude MN, Martine LO, Jules CDK, Pierre FD. 2017. Virulence Test of Some Phytophthora megakarya Isolates on Cocoa (Theobroma cacao L.) Hybrid Pods. IOSR Journal of Biotechnology and Biochemistry 3(1), 73-81.

http://dx.doi.org/10.9790/264X-03017381.

Fagbohun ED, Aderiye BI. 2017. Effects of Phytophthora Infection on Nutrient Composition of Theobroma cacao L. International Journal of Biochemistry Research & Review 16(4), 1-8.

http://dx.doi.org/10.9734/IJBCRR/2017/31872.

Harman EG, Howell RC, Viterbo A, Chet I, Lorito M. 2004. Trichoderma species – opportunistic, avirulent, plant symbionts. Nature reviews, Microbiology 2, 43-56.

http://dx.doi.org/10.1038/nrmicro797

Housti F, Andary C, Gargadenne A, Amssa M. 2002. Effects of wounding and salicylic acid on hydroxycinnamocylmalic acids in Thunbergia alata. Plant Physiology Biochemistry 40, 761-769.

Iwaro AD. 2001. Sreenivasan T.N, Butler D. R, Thevénin J.M., Mooleedhar V., Bekele F., Sounigo O., and Umaharan P. Rapid screening for Phytophthora pod rot resistance by means of detached pod inoculation. In: Eskes A.B. & Efron Y. Eds. Proceedings of the CFI/ICCO/IPGRI Projet Workshop on Workin procedures for cocoa germplasm evaluation and selection. 1-6 February 1998. Montpellier, France, p 109-111.

Lu FC, Lee CY, Wang CL. 2015. The influence of arbuscular mycorrhizal fungi infection on yam (Dioscorea spp.) tuber weights and secondary metabolite content. Peer Journal 3, e1266.

http://dx.doi.org/10.7717/peerj.1266.

Manga NJ, Effa OP, Ondobo ML, Djoko KJC, Djocgoue PF. 2016. Heritability of the tolerance to Phytophthora megakarya Bras. And Grif. of Theobroma cacao L. in terms of their necrosis length, phenolic contents and activity of enzymes. International Journal of Biosciences 8(5), 249-261.

http://dx.doi.org/10.12692/ijb/8.5.249-261.

Marigo G. 1973. Sur une méthode de fractionnement et d’estimation des composés phénoliques chez les végétaux. Analysis 2(2), 106-110.

Marx HD, Bryan WC, Cordell CE. 1977. Survival and growth of Pine seedlings with Pisolithus ectomy-corrhizae after two years on reforestation sites in North Carolina and Florida. Forest Science 3(3), 363-373.

Minyaka E, Niemenak N, Ngangue LTA, Madina BC, Bahoya JA, Omokolo ND. 2017. Peroxidase and polyphenol oxidase activities associated to somatic embryogenesis potential in an elite hybrid genotype of Theobroma cacao L. African Journal of Biotechnology 16(49), 2278-2288.

http://dx.doi.org/10.5897/AJB2017.16157.

Nana WL, Nwaga D, Fokom R, Oneya S, Ngakou A. 2002. Variation des composés phénoliques chez Vigna unguiculata (L.) Walp. (Légumineuse) et influence des rhizobia et des mycorhizes sur leur biosynthèse. African Journal of Sciences and Technology 3, 127-135.

Nana WL, Ekounda TV, Mkounga P, Eke P, Nkengfack EA, Nwaga D. 2016. Potentialisation of the biocontrol efficacy of arbuscular mycorrhizas fungi against cocoa black pod rot causing Phytophthora megakarya with natural flavonoid. International Journal of Agronomy and Agricultural Research 9(1), 165-181.

Ndoumbe-Nkeng M, Cilas C, Nyemb E, Nyasse S, Bieysse D, Flori A, Sache I. 2004 Impact of removing diseased pods on cocoa black pod caused by Phytophthora megakarya and on cocoa production in Cameroon. Crop Protection 23, 415-424.

Ngonkeu MEL. 2009. Tolérance de certaines variétés de maïs aux sols à toxicité aluminique et manganique du Cameroun et diversités moléculaire et fonctionnelle des mycorhizes à arbuscules. Thèse, Université de Yaoundé I, Cameroun 224 p.

Nyadanu D, Akromah R, Adomako B, Kwoseh C, Dzahini-Obiatey H, Lowor ST, Akrofi AY, Assuah MK. 2012. Host plant resistance to Phytophthora pod rot in cocoa (Theobroma cacao L.): The role of epicuticular wax on pod and leaf surfaces. International Journal of Botany 8(1), 13-21.

http://dx.doi.org/3923/ijb.2012.13.21.

Nyassé S, Cilas C, Herail C, Blaha G. 1995. Infection foliaire en tant que test de dépistage précoce de la résistance du cacao (T. cacao) à la maladie de la pourriture brune des cabosses par P. megakarya. Protection des cultures 14(8), 657-663.

Nzweundji JG, Tchiechoua YH, Tchotet JMT, Djocgoue PF, Omokolo DN, Niemenak N, Ngome FA, Donfagsiteli NT, Mbita Messi JCH, Ngonkeu ELM. 2015. Molecular diversity of arbuscular mycorrhizal fungi associated with Prunus africana (hook f.) Kalkman (Rosaceae) in humid forest zones of Cameroon. International Journal of Development Research 5, 5289-5297.

Ondobo ML, Effa OP, Djocgoue PF, Manga NJ, Boudjeko T, Omokolo ND. 2014. Phenolic content and heritability of resistance in four hybrid populations of Theobroma cacao L. after Leaves inoculation with Phytophthora megakarya Bras. and Grif. International Journal of Biological and Chemical Sciences 8(1), 17-30.

http://dx.doi.org/10.4314/ijbcs.v8i1.3

Ondobo LM, Effa OP, Manga NJ, Djoko KCJ, Djocgoue FP. 2017. Breeding of parental and tolerant hybrids of Theobroma cacao L. to Phytophthora megakarya Bras. and Griff. International Journal of Plant Physiology and Biochemistry 9(2), 9-21.

https://doi.org/10.5897/ijppb2017.0262

Pokou ND, N’goran JAK, Kébé I, Eskes A, Tahi M, Sangaré A. 2008. Levels of resistance to Phytophthora pod rot in cocoa accessions selected on-farm in Côte d’Ivoire. Crop Protection 27, 302-309.

Phillips JM, Hayman DS. 1970. Improved Procedures for Clearing Roots and Staining Parasitic Vesicular- Arbuscular Mycorrhizal Fungi for Rapid Assessment of Infection. Transactions of the British Mycological Society 55, 158-161.

http://dx.doi.org/10.1016/S0007-1536(70)80110-3

Shahin SA, Jonathan S, Lary DJ, Brent K, Shen D, Strem MD, Amoako-Attah I, Akrofi AY, Begoude AD, ten Hoopen GM, Coulibaly K, Kebe BI, Melnick RL, Guiltinan MJ, Tyler BM, Meinhardt LW, Bailey BA. 2017a. Phytophthora megakarya and Phytophthora palmivora, Closely Related Causal Agents of Cacao Black Pod Rot, underwent increases in genome sizes and gene numbers by different mechanisms. Genome Biology and Evolution 9(3), 536–557.

http://dx.doi.org/10.1093/gbe/evx021

Shahin SA, Jonathan S, Lary DJ, Strem MD, Meinhardt LW, Bailey BA. 2017b. Phytophthora megakarya and P. palmivora, causal agents of black pod rot, induce similar plant defense responses late during infection of susceptible cacao pods. Frontiers in Plant Science 18, 1-18.

http://dx.doi.org/10.3389/fpls.2017.00169

Simo C, Djocgoue PF, Mbouobda HD, Effa OP, Boudjeko T, Ndiang Z, Omokolo ND. 2014. Assessing relationship between phenolic compounds and resistance to Phytophthora megakarya using two cocoa (Theobroma cacao L.) families. African Journal of Biotechnology 13(9), 2956-2965.

http://dx.doi.org/10.5897/AJB2014.13747.

Taquet B. 1985. Les mécanismes physiologiques de la réaction de défense du palmier à huile contre la fusariose vasculaire. Application à la recherche de nouveaux moyens de lutte. Thèse de Doctorat 3e cycle. Université Paris 6, France 151 p.

Tchameni SN, Ngonkeu MEL, Begoude BAD, Nana WL, Fokom R, Mbarga JB, Tchana T, Tondje PR, Etoa FX, Kuaté J. 2011. Effect of Trichoderma asperellum and arbuscular mycorrhizal fungi on cacao growth and resistance against black pod disease. Crop Protection 30, 1321-1327.

http://dx.doi.org/10.1016/j.cropo.2011.05.003.

Tchameni NS, Nwaga D, Nana WL, Ngonkeu EL, Fokom R, Kuate J, Etoa FX. 2012. Growth enhancement, amino acid synthesis and reduction in susceptibility towards Phytophthora megakarya by arbuscular mycorrhizal fungi infection cocoa plants. Journal of Phytopathology 160, 220-228.

http://dx.doi.org/10.1111/j.1439-0434.2012.01888.x.

Tchameni NS, Sameza LM, O’donovanb A, Fokom R, Mangaptche NLE, Nana WL, Etoa FX, Nwaga D. 2017. Antagonism of Trichoderma asperellum against Phytophthora megakarya and its potential to promote cacao growth and induce biochemical defence. Mycology 8(2).

http://dx.doi.org/10.1080/21501203.1300199.

Trouvelot A, Kough J, Gianinazzi-Pearson V. 1986. Evaluation of VA infection levels in root systems. Research for estimation methods having a functional significance. In : V. Gianinazzi-Pearson and S. Gianinazzi (eds.), Physiological and Genetical Aspects of Mycorrhizae. INRA Press, Paris, France, p 217– 221.

Simo Claude, Fankem Henri, Njonzo-Nzo Stephanie Alvine, Tassong Saah Denis, Taffouo Victor Desire, Djocgoue Pierre François.
Effect of Glomus intraradices in improving the tolerance of a hybrid family of Theobroma cacao L. against Phytophthora megakarya.
Int. J. Biosci. 14(4), 76-94, April 2019.
https://innspub.net/ijb/effect-glomus-intraradices-improving-tolerance-hybrid-family-theobroma-cacao-l-phytophthora-megakarya/
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