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Effect of metabolite of Tamarix aphylla against liver fibrosis in Balb-c mice

By: Sanobar Gull, Razia Noreen, Shazia Anwer Bukhari, Muhammad Shareef Masoud, Muhammad Qasim

Key Words: Tamarix aphylla, Antifibrotic activity, CCl4, SGPT, SGOT.

Int. J. Biosci. 15(2), 490-502, August 2019.


Certification: ijb 2019 0109 [Generate Certificate]


The main focus of this research was the characterization of ethanol extract of Tamarix aphylla  leaf (EETAL) to explore the antioxidant profile and hepatoprotective activity against CCl4 liver injury in mice. The EETAL was used for phytochemical study; phenolics and flavonoids content (185.66± 3.05 mg GAE/g and 194.66±1.52 mg QE/g). In vivo studies, mice were allocated randomly into four groups, each group comprises of six mice. Liver was injured by CCl4 of (2-4) groups to analyze the liver marker enzyme. Lowest value of superoxide dismutase (SOD), Glutathione peroxidase and catalase (10.26±1.07, 1.05 ±0.08, 6.91 ± 0.30) in CCl4 treating group but normal in G4 group EETAL treated group (SOD, Gpx and catalase: 19.08±1.25, 2.47 ± 0.17, 12.41±0.30) remains nearer to the control group (SOD, Gpx and catalase: 25.37± 1.11, 2.96± 0.37, and 12.56±0.92) or silymarin group (SOD, Gpx and catalase: 21.18± 1.05, 2.56 ±0.15, 12.64±0.54) respectively. The values of lipid peroxidation and MDA (22.51 ± 1.15, 7.49± 0.15) were significantly higher in CCl4 treating group as compare to the normal group (LPO and MDA: 10.39± 0.05, 2.49± 0.03) and decrease in group 4 treated with EETAL (13.75 ±0.10, 2.34± 0.09). The high level of serum marker enzyme in CCl4 group (AST, ALT, ALP, and Bilirubin total: [(213.6±5.4, 264.16±5.9, 306.3±4.9, 1.41±0.3) IU/L]. The EETAL recover the serum level [(AST: 43.35±2.65, ALT: 53.5± 2.8, ALP: 178.33±2.1) IU/L]. The histopathological examination showed improvement in cellular architecture with EETAL. The results of present research demonstrated the protective effect of EETAL against liver fibrosis in mice.

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Effect of metabolite of Tamarix aphylla against liver fibrosis in Balb-c mice

Amina T, Boukhari A, Nouidjem Y. 2018. Phenolic content, HPLC analysis and Antioxidant activity extract from Tamarix Articulata.  Journal of Advance Pharmaceutical Education Resources 8(4), 1-8.

Auribie MA. 2011. Antioxidant activity of tannin from Tamarix aphylla L. leaves. Basrah Journal of Agricultural Sciences 24(1), 1-10.

Boll M, Weber LW, Becker E, Stampfl A. 2001. Mechanism of carbon tetrachloride-induced hepatotoxicity. Hepatocellular damage by reactive carbon tetrachloride metabolites. Zeitschrift fur Naturforschung C567(8), 49-59.

Brautbar N, William J. 2002. Industrial solvents and liver toxicity: Risk assessment, risk factors and mechanisms. International Journa of Hygiene and Enviornmental Health   205, 479-491.

Chang CH, Lin HY, Chang CY, Liu YC. 2006. Comparisons on the antioxidant properties of fresh, freeze-dried and hot-air-dried tomatoes. Journal of Food Engineering 77(3), 478-485.

Chaturvedi S, Drabu S, Sharma M. 2012. Anti-inflammatory and Analgesic activity of Tamarix gallica. International journal of pharmacy and pharmaceutical sciences 4(3), 653–658.

Chiu YJ, Chou SC, Kao CP, Wu KC, Chen CJ. 2018.   Hepatoprotective effect of ethanol extract of Polygonum orientale on carbon tetrachloride-induced acute liver injury in mice. Journal of food and drug Analysis 26, 369-379.

De Victorica J. Galván M. 2001.Pseudomonas aeruginosa as an indicator of health risk in water for human consumption. Water Science and Technology 43, 49–52.

Desai SN, Patel DK, Devkar RV, Patel PV, Ramachandran AV. 2012. Hepatoprotective potential of polyphenolrich extract of Murraya koenigii L. An in vivo study. Food and Chemical Toxicology  50(2), 410-314.

Emad AM, Gamal EE. 2013. Screening for Antimicrobial Activity of some plants from Saudi Folk Medicine. Global Journal of Research on Medicinal Plants and Indigenous Medicine 2, 189–197.

Farah N, Syeda Q, Zabta KS, Azhar A, Irtifaq A. 2013.  Phytochemical Investigations of Tamarix Indica Willd. And Tamarix Passernioides Del. Ex Desv. Leaves from Pakistan. Pakistan journal of Botany 45(5), 1503-1507.

Garcia N, Zazueta C. 2015. Ellagic acid: Pharmacological activities and molecular mechanism involved in liver protection. Pharmacological Research 97, 84-103.

Hussain AI,   Chatha SA, Noor SK, Khan ZA. 2012. Effect of extraction techniques and solvent systems on the extraction of antioxidant components from Peanut (Arachis hypogaea L.) Hulls. Food Analytical Methods 5, 890-896.

Jaeschke H, Gores GJ, Cederbaumai Hinson JA, Pessayre D, Lemasters JJ. 2002. Mechanisms of hepatotoxicity. Toxicological Sciencces 65(2), 166-76.

Juan-Juan C, Qing LV, Bao Z   Han-Qing C. 2019. Structural characterization and hepatoprotective activities of polysaccharides from the leaves of Toona sinennsis (A.Juss) Roem. Carbohydrate polymers 212, 89-101.

Kamila Z, Agnieszka D, Kolodziejczak A, Rotsztejn H. 2018. Antioxidant properties of ferulic Acid and its possible Application. Skin Pharmacology and physiology 31, 332–336.

Khoddami MA, Wilkes TH, Roberts. 2013. Techniques for analysis of plant phenolic compounds. Molecules 18, 2328-2375.

Kim T, Lee HK, Song IB, Lim JH, Cho ES, Son HY, Jung JY, Yun H. 2013. Platycodin D attenuates bile duct ligation-induced hepatic injury and fibrosis in mice. Food and Chemical Toxicology 51, 364-369.

Knockaert L, Berson A, Ribault C, Prost PE, Fautrel A,  Pajaud J. 2012. Carbon tetrachloride mediated lipid peroxidation induces early mitochondrial alteration in mouse liver. Laboratory Investigation 92, 396-410.

Kuppan N, Shyamala M, Chen Y, Lachimanan YL, Subramanion L,  Sreenivasan S. 2011. Hepatoprotective potential of Clitoria ternatea Leaf Extract against paracetamol induced damage in mice. Molecules 16, 10134-10145.

Lefahal  M, Benahmed M, Louaar S, Zallagui A, Duddeck H, Medjroubi K, Akkal S. 2010. Antimicrobial activity of Tamarix gallica L. extracts and isolated flavonoids. Advance in Natural and Applied Sciences 4(3), 289-292.

Liu YY, Lu BN, Peng  JY. 2011. Hepatoprotective activity of the total flavonoids from Rosa leavigata Michx fruit in mice treated by paracetamol. Food Chemistry 125, 719-725.

Lu Y,  Hu D, Zhao X, Wang S, Wei G, Wang J. 2016.  Protective effect of wedelolactone against CCl4-induced acute liver injury in mice. International Immunopharmacology  34, 44-52.

Lu B, Xu Y, Xu L, Cong X, Yin L, Li H,  Peng J. 2012. Mechanism investigation of dioscin against CCl4-induced acute liver damage in mice. Environmental Toxicology and Pharmacology 34(2), 127-135.

Mahfoudhi A, Abdelkader BS, Meriem G, Saousan G, Gorcii MM,  Maha Z.  2016. Antioxidant and antimicrobial activities of Tamarix aphylla Karst growing in Tunisia. Mor. Journal of Chemistry 4, 987-995.

Mahfoudhi A, Prencipe FP, Mighri Z, Pellati F. 2014. Metabolite profiling of polyphenols in the Tunisian plant Tamarix aphylla (L.) Karst. Journal of Pharmaceutical and Biomedical Analysis  99, 97-105.

Malhi H, Gores GJ.  2008. Cellular and Molecular Mechanism of Liver Injury. Gastroenterology 134(6), 1641-1654.

Marwat SK, Khan MA, Aslam KM, Rehman F, Ahmad M, Zafar, M. 2008. Salvadora persica, Tamarix aphylla and Zizyphus mauritiana: Three Woody Plant Species Mentioned in Holy Quran and Ahadith, and their Ethnobotanical Uses in North Western Part (D.I. Khan) of Pakistan. Ethnobotanical Leaflet 12, 1013-21.

Mcgill MR, Williams CD, Xie Y, Ramachandran A, Jaeschke H. 2012. Acetaminophen-induced liver injury in rats and mice:Comparison of protein adducts, mitochondrial dysfunction, and oxidative stress in the mechanism of toxicity. Toxicology and Applied Pharmacology 264(3), 387-394.

Mohsin A, Shah AH, Al-Yahya MA, Tariq MM, Tanira OM, Ageel AM. 1989.  Analgesic, Antipyretic Activity and Phytochemical Screening of Some Plants Used in Traditional Arab System of Medicine, Fitoterapia 60, 174-177.

Nagalekshmi R, Aditya M, Dhanya K, Chandrasekharan Cherupally KN. 2011. Hepatoprotective activity of Andrographis Paniculata and Swertia Chirayita. Food and Chemical Toxicology 49, 3367-3373.

Nasir GA, Mohsin S, Khan M, Shams S, Ali G, Riazuddin S. 2013. Mesenchymal stem cells and Interleukin-6 attenuate liver fibrosis in mice. Journal of Translational Medicine   11, 78.

Nilesh J, Abhay KS. 2012. Hepatoprotective activity of chenopodium album Linn. In vitro and in vivo studies. Journal of Experimental and Integrative Medicine 2, 331-336.

Novo  E,  Cannito S, Morello  E, Paternostro C,  Bocca  C, Miglietta A, Parola M. 2015. Hepatic myofibroblasts and fibrogenic progression of chronic liver diseases. Histology and Histopathology 30, 1011-1032. (PMID: 25896393)

Panhwar AQ, Abro H. 2007. Ethno botanical studies of Mahal kohistan (khirthar national park). Pakistan Journal of Botany 39(7), 2301-2315.

Prakash S, Joshi YK. 2004. Assessment of micronutrient antioxidants, total antioxidant capacity and lipid peroxidation levels in liver cirrhosis. Asia Pacific Journal of Clinical Nutrition 13, S110.

Rajib A, Monirul I, Musaddik A, Haque E.  2009. Hepatoprotective activity of methanol extract of some medicinal plants against carbon tetrachloride induced hepatotoxicity in albino rats. European Journal of Scientific Research 3(3), 302-310.

Ramesh KG, Talib H, Panigrahi G, Avik D, Gireesh NS, Faiyazuddin MD,  Chandana VR. 2011. Hepatoprotective effect of Solanum xanthocarpum fruit extract against CCl4 induced actute toxicity in experimental animals. Asian Pacific Journal of Tropical Biomedicine 964-968.

Raza H, John A, Benedict S. 2011. Acetyl salicylic acid-induced oxidative stress, cell cycle arrest, apoptosis and mitochondrial dysfunction in human hepatoma HepG2 cells. European Journal of Pharmacology 668, 15-24.

Sekkien A, Swilam N, Ebada S, Esmat A, El-Khatib AH, Linscheid MW,  Singab AN.  2018. Polyphenols from Tamarix nilotica: LC–ESI-MSn Profiling and In Vivo Antifibrotic Activity. Molecules 23(6), 1411.

Steel RGD, Torrie JH, Dickey D. 1997. Principles and procedures of statistics, a biometrical approach. New York: McGraw Hill Bok Co Inc: USA.

Sultana B, Anwar F, Rafique AM, Chatha SAH. 2008. Antioxidant potential of extract from different agro wastes, stabilization of corn oil. Grasas Y Aceites 59, 205-17.

Surendra KR, Sharma S, Neeru V. 2012. Hepatoprotective activity of Vitis Vinifera root extract against carbon tetra chloride induced liver damage in rat. Acta poloniae pharmaceutica-Drug Research 69, 933-937. (PMID: 23061290).

Vijay N, Padmaa MP. 2011. Hepatoprotective Activity of Chenopodium Album Linn. Against alcohol induced liver damage. Phytomedicine   3, 511-523.

Yusufoglu HS, Algasoumi SI. 2011. Anti-inflammatory and Wound Healing Activities of Herbal Gel Containing an Antioxidant Tamarix aphylla Leaf Extract. International Journal of Pharmacology 7(8), 829-835.

Zhan YY, Wang JH, Tian X, Feng SX, Xue L,  Tian LP. 2016. Protective effects of seed melon extract on CCl4-induced hepatic fibrosis in mice. Journal of Ethnopharmacology 193, 531-537.

Sanobar Gull, Razia Noreen, Shazia Anwer Bukhari, Muhammad Shareef Masoud, Muhammad Qasim.
Effect of metabolite of Tamarix aphylla against liver fibrosis in Balb-c mice.
Int. J. Biosci. 15(2), 490-502, August 2019.
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