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Effect of methoxyfenozide on synthesis of major proteins in ovaries of Ephestia kuehniella Zell. (Lepidoptera: Pyralidae)

By: Kirane-Amrani Leila, Bakli Djihen, TazirAsma, Soltani Noureddine

Key Words: Ephestia kueuhniella, Ovaries proteins, Ecdysone agonist, Methoxyfenozide.

Int. J. Biosci. 12(4), 16-22, April 2018.

DOI: http://dx.doi.org/10.12692/ijb/12.4.16-22

Certification: ijb 2018 0286 [Generate Certificate]

Abstract

Insect growth regulators (IGRs) belong to a class of compounds which interfere with normal growth, development and reproduction of insects. Through greater selectivity of action IGRs have less undesirable effects on man, wild life and environment. Many of the IGRs mimic the action of insect hormones, ecdysone or juvenile hormone (JH). Methoxyfenozide is a potent non-steroidal ecdysone agonist developed as an insecticide and is effective against lepidopteran pests. The effects of methoxyfenozide (RH-2485) on reproduction of the Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae), an important pest in stored products worldwide, were evaluated under laboratory conditions. Treatment at LD50 (0.01 μg/pupa) and LD90 (0.37 μg/pupa) was made by topical applications on newly ecdysed female pupae. Data showed that methoxyfenozide, significantly affected reproductive parameters in the ovaries in treated females such as amounts of proteins, vitellogenin and vitellin as compared to the control series. In addition, electrophoretic separation of ovarian proteins by sodium dodecyl sulfate polyacrylamide slab gels (SDS-PAGE) showed that the treatment with the ecdysteroid agonist resulted in reduction in the number and the intensity of some proteins bands compared to controls. The major proteins in the ovaries of lepidopteran insects are members of the storage protein family and as storage proteins are crucial for insect development they may be targeted for developing better insect control strategies.

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Effect of methoxyfenozide on synthesis of major proteins in ovaries of Ephestia kuehniella Zell. (Lepidoptera: Pyralidae)

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Kirane-Amrani Leila, Bakli Djihen, TazirAsma, Soltani Noureddine.
Effect of methoxyfenozide on synthesis of major proteins in ovaries of Ephestia kuehniella Zell. (Lepidoptera: Pyralidae).
Int. J. Biosci. 12(4), 16-22, April 2018.
https://innspub.net/ijb/effect-methoxyfenozide-synthesis-major-proteins-ovaries-ephestia-kuehniella-zell-lepidoptera-pyralidae/
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