Effect of dietary lipids on liver lipid profile and antioxidant enzymes in male Wistar rats
By: Amal Alaoud, Dina M. Trabzuni, Shaista Arzoo
Key Words: Monounsaturated fatty acids (MUFA); polyunsaturated fatty acids (PUFA); olive; hazelnut; lipids; antioxidants.
Int. J. Biosci. 21(1), 8-18, July 2022.
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This study evaluated the effect of olive and hazelnut oil on liver lipid profile and antioxidant status in adult rats. A total of fifteen male wistar rats (150-170 grams) were randomized into three groups. Control group received 30 grams diet daily with soybean oil. OLVO and HAZO groups received 30 grams diet with olive and hazelnut oil, respectively. Total cholesterol, triglycerides, HDL-C, LDL-C, SOD, GSH-PX, catalase activity and MDA was determined in liver homogenates. The result demonstrates significant (p≤0.05) gain in animal body weight in OLVO group compared to control group. The lowest total cholesterol (2.49±0.17 mg/dl) was observed in HAZO group and difference was significant (p≤0.05) between control and treatment groups but insignificant within treated groups. Similarly, lowest value of triglyceride (11.48±0.78 mg/dl), LDL-C (6.04±0.41 mg/dl) and VLDL-C (2.29±0.14 mg/dl) and the highest HDL value (29.59±1.99 mg/dl) was also observed in OLVO group and a significant (p≤0.05) difference was found between control and treated group. Ratio LDL/ HDL and cholesterol/ HDL was lower in OLVO group, followed by HAZO and control. The highest activity of GSHPx was observed in OLVO (17.02 ±0.50U/ml) group, but the results showed insignificant (p≥0.05) differences between control and treated group. Similarly, highest concentration of SOD (5.707±0.53 U/ml) and catalase (19.68 ±1.30nmol/min/ml) and least concentration of MDA (0.67 nmol/ml) was also observed in OLVO. MUFA diets were found to have a beneficial effect on lipid profiles and antioxidant enzymes and may be used as nutritional alternatives to prevent lipid disturbances.
Effect of dietary lipids on liver lipid profile and antioxidant enzymes in male Wistar rats
Aguilera CM, Mesa MD, Ramirez-Tortosa MC,
Nestares MT, Ros E, Gil A. 2004. Sunflower oil does not protect against LDL oxidation as virgin olive oil does in patients with peripheral vascular disease. Clinical Nutrition 23, 673-81.
Aparicio R. 2000. Characterization: mathematical procedures for chemical analysis. In: Harwood J, Aparicio R (eds) Handbook of olive oil: analysis and properties. Aspen, Gaithersburg, Maryland Md, p 285–54.
Aquino J de S, Tavares RL, Medeiros Lde B, Martins CC, Pessoa DC, Stamford TL. 2015. Effect of the consumption on buriti oil on the metabolism of rats induced by iron overload. Archives of Endocrinology and Metabolism 59, 422-427.
Arroyave-Ospina JC, Wu Z, Geng Y, Moshage H. 2021. Role of oxidative stress in the pathogenesis of nonalcoholic fatty liver disease: Implications for prevention and therapy. Antioxidants 10, 174.
Baum SJ, Kris-Etherton PM, Willett WC, Lichtenstein AH, Rudel LL, Maki KC, Whelan J, Ramsden CE, Block RC. 2012. Fatty acids in cardiovascular health and disease: A comprehensive update. Journal of Clinical Lipidology 6, 216-234.
Berry EM, Eisenberg S, Haratz D, Friedlander Y, Norman Y, Kaufmann NA. 1991. Effect of diets rich in monounsaturated fatty acids on plasma lipoproteins- the Jerusalem Nutrition Study: high MUFAs vs. high PUFAs. American Journal of Clinical Nutrition 53, 899–907.
Boudalia S, Berges R, Chabanet C, Folia M, Decocq L, Pasquis B, Najar LA, Lavier MCC. 2014. A multi-generational study on low-dose BPA exposure in Wistar rats: Effects on maternal behavior, flavor intake and development. Neurotoxicology and Teratology 41, 16-26.
Caimari A, Puiggròs F, Suárez M, Crescenti A, Laos S, Ruiz JA, Alonso V, Moragas J, Bas JMD, Arola L. 2015. The intake of a hazelnut skin extract improves the plasma lipid profile and reduces the lithocholic/deoxycholic bile acid faecal ratio, a risk factor for colon cancer, in hamsters fed a high-fat diet. Food Chemistry 15, 138–44.
Chang NW, Huang PC. 1999. Comparative effects of polyunsaturated to saturated fatty acid ratio versus polyunsaturated and monounsaturated fatty acids to saturated fatty acid ratio on lipid metabolism in rats. Artherosclerosis 142, 185-191.
de la Torre-Carbot K, Chávez-Servín JL, Reyes P, Ferriz RA, Gutiérrez E, Escobar, K, Aguilera A, Anaya MA, García-Gasca T, GarcíaOP, Rosado JL. 2015. Changes in lipid profile of Wistar Rats after sustained consumption of different types of commercial vegetable oil: A preliminary study. Universal Journal of Food and Nutrition Science 3, 10-18.
Degirolamo C, Rudel LL. 2010. Dietary monounsaturated fatty acids appear not to provide cardioprotection. Current Atherosclerosis Report 12, 391-396.
Diane A, Victoriano M, Fromentin G. 2008. Acute stress modifies food choice in Wistar male and female rats. Appetite 50, 397-07.
Diekman C, Malcolm K. 2009. Consumer perception and insights on fats and fatty acids: knowledge on the quality of diet fat. Annals of Nutrition and Metabolism 54(1), 25–32.
Duavy SMP, Salazar GJT, Leite G de O, Ecker A, Barbosa NV. 2017. Effect of dietary supplementation with olive and sunflower oils on lipid profile and liver histology in rats fed high cholesterol diet. Asian Pacific Journal of Tropical Medicine 10, 539-543.
El-Kholy TA, Abu Hilal M, Al-Abbadi HA, Serafi AS, Al-Ghamdi AK, Sobhy HM, Richardson JRC. 2014. The effect of extra virgin olive oil and soybean on DNA, cytogenicity and some antioxidant enzymes in rats. Nutrients 6, 2376-2386.
Forsberg L, de Faire U, Morgenstern R. 2001. Oxidative stress, human genetic variation and disease. Archives of Biochemistry and Biophysics 389, 84-93.
Garg A. 1998. High-monounsaturated-fat diets for patients with diabetes mellitus: a meta-analysis. American Journal of Clinical Nutrition 67, 577S–82S.
GBD 2016 Causes of Death Collaborators. 2017. Global, regional and national age-sex specific mortality for 264 causes of death, 1980–2016: a systematic analysis for the Global Burden of Disease Study 2016. Lancet 390, 1151–1210.
GBD 2017. Diet Collaborators. 2019. Health effects of dietary risks in 195 countries, 1990–2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet 393, 1958–1972.
Gorinstein S, Leontowicz H, Lojek A,
Leontowicz M, Ciz M, Krzeminski R Gralak M, Czerwinski J, Jastrzebski Z, Trakhtenberg S, Grigelmo-Miguel N, Soliva-Fortuny R, Martin-Belloso O. 2002. Olive oils improve lipid metabolism and increase antioxidant potential in rats fed diets containing cholesterol. Journal of Agricultural and Food Chemistry 50, 6102–08.
Gutteridge JM, Halliwell B. 2000. Free radicals and antioxidants in the year 2000: A historical look to the future. Ann N Y Acad Science 899, 136-47.
Hatipoglu A, Kanbagli Ö, Balkan J, Küçük M, Cevikbaş U, Aykaç-Toker G, H Berkkan, M Uysal. 2004. Hazelnut oil administration reduces aortic cholesterol accumulation and lipid peroxides in the plasma, liver, and aorta of rabbits fed a high-cholesterol diet. Bioscience Biotechnology and Biochemistry 68, 2050-57.
Hayes KC, Pronezuk A, Lindsey S, Diersen Schade D. 1994. Dietary fatty acids (12:0, 14:0, 16:0) differ in their impact on plasma cholesterol and lipoproteins in non-human primates. American Journal of Clinical Nutrition 53, 491-98.
Heyden S. 1994. Polyunsaturated and monounsaturated fatty acids in the diet to prevent coronary heart disease via cholesterol reduction. Annals of Nutrition and Metabolism 38, 117–122.
Hu FB, Willett WC. 2002. Optimal diets for prevention of coronary heart disease. JAMA 288, 2569–78.
Institute of Medicine. 2005. Dietary Reference Intakes for Energy, Carbohydrate, Fiber, Fat, Fatty Acids, Cholesterol, Protein, and Amino Acids. Washington, DC: The National Academies Press.
Jean C, Fromentin G, Tomé D, Larue-Achagiotis C. 2002. Wistar rats allowed to self-select macronutrients from weaning to maturity choose a high-protein, high-lipid diet. Physiology and Behavior 76, 65-73.
Jenkins WM, Jenkins AE, Brydson C. 2020. The portfolio diet for cardiovascular disease risk reduction. An evidence based approach to lower cholesterol through plant food consumption. Chapter 3 – How It Works: Mechanisms of Action. Editor(s): Jenkins WM, Jenkins AE, Jenkins AL, Brydson C, p 29-46.
Kelly JH, Sabaté J. 2006. Nuts and coronary heart disease: An epidemiological perspective. British Journal of Nutrition 96, S61–S67.
Kratz M, Cullen P, Kannenberg F, Kassner A, Fobker M, Abuja PM. 2002. Effects of dietary fatty acids on the composition and oxidizability of low-density lipoprotein. European Journal of Clinical Nutrition 56, 72-81.
Łuczaj W, Gęgotek A, Skrzydlewska E. 2017. Antioxidants and HNE in redox homeostasis. Free Radical Biology and Medicine 111, 87-101.
Madigan C, Ryan M, Owens D, Collins P, Tomkin GH. 2005. Comparison of diets high in monounsaturated versus polyunsaturated fatty acid on postprandial lipoproteins in diabetes. Irish Journal of Medical Science 174, 8-20.
Marai I, Massalha S. 2014. Effect of omega-3 polyunsaturated fatty acids and vitamin D on cardiovascular diseases. Israel Medical Association Journal 16, 117-121.
Mercanlıgil S, Arslan P, Alasalvar C, Okut E, Akgul E, Pinar A, Geyik PÖ, Tokgözoğlu L, Shahidi F. 2007. Effects of hazelnut-enriched diet on plasma cholesterol and lipoprotein profiles in hypercholesterolemic adult men. European Journal of Clinical Nutrition 61, 212–220.
Millan J, Pinto X, Munoz A, Zúñiga M, Rubiés-Prat J, Pallardo LF. 2009. Lipoprotein ratios: Physiological significance and clinical usefulness in cardiovascular prevention. Vascular Health and Risk Management 5, 757-65.
Morello, JR, Motliva MJ, Tovar MJ, Romero MP. 2007. Changes in commercial virgin oil during storage with special emphasis on the phenolic fraction. Journal of Agricultural and Food Chemistry 85, 357-64.
Nakbi A, Tayeb W, Grissa A, Issaoui M, Dabbou S, Chargui I, Ellouz M, Miled A, Hammami M. 2010. Effect of olive oil and its fraction oxidative stress and the livers fatty acid composition in 2,4-dichlorophenoxyaccetic treated rats. Nutrition and Metabolism 7, 80.
Nardini M, D’Aquino M, Tomassi G, Gentili V, Di Felice M, Scaccini C. 1995. Dietary fish oil enhances plasma and LDL oxidative modification in rats. Journal of Nutritional Biochemistry 6, 474–80.
Natoli S, McCoy P. 2007. A review of the evidence: Nuts and body weight. Asia Pacific Journal of Clinical Nutrition 16, 588–97.
Parthasarathy S, Khoo JC, Miller E, Barnett J, Witzturn J, Steinberg D. 1990. Low density lipoprotein rich in oleic acid is protected against oxidative modification: Implications for dietary prevention of atherosclerosis. Proceedings of the National Academy of Sciences, USA 87, 3894-98. http://dx.doi.org/10.1073/pnas.87.10.3894
Petersen JM, Oosthuizen W, Jerling JC. 2005. A systematic review of the effects of nuts on blood lipid profiles in humans. Journal of Nutrition 135, 2082–2089.
Reaven P, Parthasarathy S, Grasse BJ, Miller E, Steinberg D, Witztum JL. 1993. Effects of oleate rich and linoleate rich diets on the susceptibility of low density lipoprotein to oxidative modification in mildly hypercholesterolemic subjects. Journal of Clinical Investigation 91, 668–76.
Ros E, Núñez I, Pérez-Heras A, Serra M, Gilabert R, Casals E, Deulofeu R. 2004. A walnut diet improves endothelial function in hypercholesterolemic subjects: A randomized crossover trial. Circulation 109, 1609–1614.
Sabate J. 2003. Nut consumption and body weight. American Journal Clinical Nutrition 78, 647S–50S.
Scaccini C, Nardini M, D’Aquino M, Gentili V, Di Felice M, Tomassi G. 1992. Effect of dietary oils on lipid peroxidation and on antioxidant parameters of rat plasma and lipoprotein fractions. Journal of Lipid Research 33, 627-33.
Schwingshackl L, Strasser B, Hoffmann G. 2011. Effects of monounsaturated fatty acids on cardiovascular risk factors: a systematic review and meta-analysis. Annals of Nutrition and Metabolism 59, 176-186.
Trautwein EA, Rieckhoff D, Kunath-Rau A,
Erbersdobler HF. 1999. Replacing saturated fat with PUFA-Rich (Sunflower Oil) Or MUFA-rich (rapeseed, olive and high-oleic sunflower oil) fats resulted in comparable hypocholesterolemic effects in cholesterol-fed hamsters. Annals of Nutrition and Metabolism 43, 159-72.
Uauy R. 2009. Dietary fat quality for optimal health and well-being: overview of recommendations. Annals of Nutrition and Metabolism 54(1), 2-7.
Wamoto M, Imaizumi K, Sato M, Hirooka Y, Sakai K, Takeshita A, Kono M. 2002. Serum lipid profiles in Japanese women and men during consumption of walnuts. European Journal of Clinical Nutrition 56, 629–37.
Weech M, Vafeiadou K, Hasaj M, Todd, S, Yaqoob P, Jackson KG, Lovegrove JA. 2014. Development of a food-exchange model to replace saturated fat with MUFAs and n-6 PUFAs in adults at moderate cardiovascular risk. Journal of Nutrition 144, 846-855.
World Health Organization. 2021. Non-communicable diseases. Accessed from- https://www.who.int/news-room/fact-sheets/detail/noncommunicable-diseases.
Effect of dietary lipids on liver lipid profile and antioxidant enzymes in male Wistar rats.
Int. J. Biosci. 21(1), 8-18, July 2022.
By Authors and International Network for
Natural Sciences (INNSPUB)