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Effectiveness of Piper betel leaf extracts against Acinetobacter species isolated from bronchitis and pharyngitis patients

By: Sehr Syed, Umair Azmat, Shaista Ilyas, Arslan Fazal, Tanzeela Murmim, Fakhra Riaz, Maryam Khan, Saba Shamim

Key Words: Bronchitis; pharyngitis; Acinetobacter; Piper betel leaf; GC-MS analysis.

Int. J. Biosci. 17(5), 162-175, November 2020.


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Acinetobacter species is associated with healthcare-associated infections especially chronic cough and other related complications. They are becoming increasingly antibiotic resistant. In the present work, the antimicrobial activity of different extract (water, ethanol, methanol, and chloroform) of Piper betel leaves were tested against Acinetobacter sp. SZ-1 and Acinetobacter baumanii TM-1 isolated from clinical samples collected from the patients suffering from pharyngitis, bronchitis and sore throat. For the antibacterial activity, different plant extracts were used in which Piper betel leaf stand out to be the most effective. Six solvents were used for this study in which only chloroformic extract showed the zones of inhibition. TLC analysis showed five spots with Rf 0.896,0.973,0.747,0.574 and 0.482. SDS-PAGE revealed proteins of different molecular weight when the bacterial cells were treated with ethanolic extract. It was also confirmed by Bradford analysis. GCMS analysis depicted different bioactive compounds including phytol; phenol,2,2-methylenebis[6-(1,1-dimethylethyl)-4-methyl; spirost-8-en-11-one,3-hydroxy-,(3β,5α,14β,20β,22β,25R) and 2,2,4-trimethyle-3-(3,8,12,16-tetramethyl-heptadeca-3,7,11,15-tetraenyl)-cyclohexanol. Furthermore, in silico analysis can enhance the existing knowledge about establishing the significance of P. betel leaves as an effective drug to treat the acute and chronic upper respiratory tract infections.

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Effectiveness of Piper betel leaf extracts against Acinetobacter species isolated from bronchitis and pharyngitis patients

Abbo A, Carmeli Y, Navon-Venezia S, Siegman-Igra Y, Schwaber M. 2007. Impact of multi-drug-resistant Acinetobacter baumannii on clinical outcomes. European Journal of Clinical Microbiology and Infectious Diseases 26(11), 793-800.

Abrahim NN, Kanthimathi MS, Adbul-Aziz A. 2012. Piper betle shows antioxidant activities, inhibits MCF-7 cell proliferation and increases activities of catalase and superoxide dismutase. BMC Complementary and Alternative Medicine 12, 220-231.

Agarwal T, Singh R, Shukla AD, Waris I, Gujrati A. (2012). Comparative analysis of antibacterial activity of four Piper betel varieties. Advances in Applied Sciences Research 3(2), 698-705.

Al Atrouni A, Joly-Guillou M-L, Hamze M, Kempf M. 2016. Reservoirs of non-baumannii Acinetobacter species. Frontiers in Microbiology 7, 49.

Albert RH. 2010. Diagnosis and treatment of acute bronchitis. American Family Physician 82(11), 1345-1350.

Albrecht MC, Griffith ME, Murray CK. 2006. Impact of Acinetobacter infection on the mortality of burn patients. Journal of the American College of Surgeons 203, 546-550.

Aliahmat NS, Yusof MRM, Wan WJ, Ngah MS, Wan WZ, Yusof YAM. 2012. Antioxidant enzyme activity and malondialdehyde levels can be modulated by Piper betle, tocotrienol rich fraction and Chlorella vulgaris in aging C57BL/6 mice. Clinics (Sao Paulo) 67(12), 447-1454.

Bauer AW, Kirby WM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology 45(4), 493-496.

Boldy DA, Skidmore SJ, Kristinsson KG, Ayres JG. 1990. Acute bronchitis in the community: Clinical features, infective factors, changes in pulmonary function and bronchial reactivity to histamine. Respiratory Medicine 84, 377-385.

Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248-254.

Chakraborty D, Shah B. 2011. Antimicrobial, antioxidative and antihemolytic activity of Piper betel leaf extracts. International Journal of Pharmacy and Pharmaceutical Sciences 3, 192-199.

Chauhan ES, Aishwarya J, Singh A, Tiwari A. 2016. A review: nutraceuticals properties of Piper betel (Paan). American Journal of Phytomedicine and Clinical Therapeutics 4(02), 028-041.

Cheesbrough M. 2006. District laboratory practice in tropical countries, part 2. Cambridge university press.

Center for Disease Control (CDC). 2004. Acinetobacter baumannii infections among patients at military medical facilities treating injured US service members, 2002-2004. Morbidity and Mortality Weekly Report 53, 1063.

Datta A, Ghoshdastidar S, Singh M. 2011. Antimicrobial property of Piper betel leaf against clinical isolates of bacteria. International Journal of Pharma Sciences and Research 2, 104-109.

Doughari HJ, Ndakidemi PA, Human IS, Benade S. 2011. The ecology, biology and pathogenesis of Acinetobacter spp.: an overview. Microbes and Environments 26(2), 101-112.

Dwivedi B, Mehta B. 2011. Chemical investigation of aliphatic compounds of Piper betle (leaf stalk). Journal of Natural Product and Plant Resources 1, 18-24.

Eliopoulos GM, Maragakis LL, Perl TM. 2008. Acinetobacter baumannii: epidemiology, antimicrobial resistance, and treatment options. Clinical Infectious Diseases 46(8), 1254-1263.

Evans B, Hamouda A, GB Amyes S. 2013. The rise of carbapenem-resistant Acinetobacter baumannii. Current Pharmaceutical Design 19, 223-238.

Guha P. 2006. Betel leaf: the neglected green gold of India. Journal of Human Ecology 19, 87-93.

Hildreth AF, Takhar S, Clark MA, Hatten B. 2015. Evidence-based evaluation and management of patients with pharyngitis in the emergency department. Emergency Medicine Practice 17(9), 1-16.

Howard A, O’Donoghue M, Feeney A, Sleator RD. 2012. Acinetobacter baumannii: an emerging opportunistic pathogen. Virulence 3(3), 243-250.

Ishnava KB, Chauhan JB, Barad MB. 2013. Anticariogenic and phytochemical evaluation of Eucalyptus globules Labill. Saudi Journal of Biological Sciences 20(1), 69-74.

Jung J, Park W. 2015. Acinetobacter species as model microorganisms in environmental microbiology: current state and perspectives. Applied Microbiology and Biotechnology 99(6), 2533-2548.

Kamolvit W, Sidjabat HE, Paterson DL. 2015. Molecular epidemiology and mechanisms of carbapenem resistance of Acinetobacter spp. in Asia and Oceania. Microbial Drug Resistance 21, 424-434.

Kaveti B, Tan L, Sarnnia, Kuan TS, Baig M. 2011. Antibacterial activity of Piper betel leaves. International Journal of Pharmaceutical Teaching Practical 2(3), 129-132.

Khan JA, Kumar N. 2011. Evaluation of antibacterial properties of extracts of Piper betel leaf. Journal of Pharmaceutical and Biomedical Sciences 11, 1-3.

Kim V, Criner GJ. 2013. Chronic bronchitis and chronic obstructive pulmonary disease. American Journal of Respiratory and Critical Care Medicine 187(3), 228-237.

Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.

Liu YC, Lee MR, Chen CJ, Lin YC, Ho HC. 2015. Purification of Cu/Zn superoxide dismutase from Piper betle leaf and its characterization in the oral cavityJournal of Agricultural and Food Chemistry 63(8), 2225-2232.

Lolans K, Rice TW, Munoz-Price LS, Quinn JP. 2006. Multicity outbreak of carbapenem-resistant Acinetobacter baumannii isolates producing the carbapenemase OXA-40. Antimicrobial Agents and Chemotherapy 50, 2941-2945.

Louie JK, Hacker JK, Gonzales R, Mark J, Maselli JH, Yagi S, Drew WL. 2005. Characterization of viral agents causing acute respiratory infection in a San Francisco University Medical Center clinic during the influenza season. Clinical Infectious Diseases 41, 822-828.

Nalina T, Rahim Z. 2007. The crude aqueous extract of Piper betle L. and its antibacterial effect towards Streptococcus mutans. American Journal of Biotechnology and Biochemistry 3, 10-15.

Nouri L, Nafchi AM, Karim A. 2014. Phytochemical, antioxidant, antibacterial, and α-amylase inhibitory properties of different extracts from betel leaves. Industrial Crops and Products 62, 47-52.

Park JY, Park S, Lee SH, Lee MG, Park YB, Oh KC, Lee JM, Kim D, Seo KH, Shin KC, Yoo KH, Ko Y, Jang SH, Jung KS, Hwang Y. 2016. Microorganisms causing community-acquired acute bronchitis: The role of bacterial infection. PLoS One 11(10), e0165553.

Petrovska BB. 2012. Historical review of medicinal plants’ usage. Pharmacognosy Reviews 6(11), 1.

Quisumbing E. 1978. Medicinal Plants of the Philippines. Quezon City, Philippines. Katha Publishing Co., Inc.

Ramji N, Ramji N, Iyer R, Chandrasekaran S. 2002. Phenolic antibacterials from Piper betle in the prevention of halitosis. Journal of Ethnopharmacology 83(1-2), 149-152.

Rodríguez-Baño J, García L, Ramírez E, Martínez-Martínez L, Muniain MA, Fernández-Cuenca F, Beltrán M, Gálvez J, Rodríguez JM, Velasco C, Morillo C, Perez F, Endimiani A, Bonomo RA, Pascual A. 2009. Long-term control of hospital-wide, endemic multidrug-resistant Acinetobacter baumannii through a comprehensive “bundle” approach. American Journal of Infection Control 37(9), 715-722.

Row LCM, Ho JC. 2009. The antimicrobial activity, mosquito larvicidal activity, antioxidant property and tyrosinase inhibition of Piper betle. Journal of the Chinese Chemical Society 56(3), 653-658.

Rukshana M, Doss A, Kumari P. 2017. Phytochemical screening and GC-MS analysis of leaf extract of Pergularia daemia (Forssk) Chiov. Asian Journal of Plant Science and Research 7, 9-15.

Sambrook J, Russell D. 2001. Molecular Cloning: A Laboratory Manual. 3rd Ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.

Schulz V, Hänsel R, Tyler VE. 2001. Rational phytotherapy: a physician’s guide to herbal medicine. Psychology Press.

Sebeny PJ, Riddle MS, Petersen K. 2008. Acinetobacter baumannii skin and soft-tissue infection associated with war trauma. Clinical Infectious Diseases 47(4), 444-449.

Seifert H, Strate A, Pulverer G. 1995. Nosocomial bacteremia due to Acinetobacter baumannii. Clinical features, epidemiology, and predictors of mortality. Medicine 74(6), 340-349.

Shamim S, Khan M. 2017. Phytochemical screening by high performance liquid chromatography (HPLC) and antimicrobial activity of different solvent fractions of areca nuts against Bacillus subtilis biofilm. International Research Journal of Pharmacy 8, 39-37.

Shamim S, Rehman A. 2015. Antioxidative enzyme profiling and biosorption ability of Cupriavidus metallidurans CH34 and Pseudomonas putida mt2 under cadmium stress. Journal of Basic Microbiology 55, 374-381.

Subashkumar R, Sureshkumar M, Babu S, Thayumanavan T. 2013. Antibacterial effect of crude aqueous extract of Piper betle L. against pathogenic bacteria. International Journal of Research in Pharmaceutical and Biomedical Sciences 4(1), 42-46.

Valle DL, Puzon JJM, Cabrera EC, Rivera LW. 2016. Thin layer chromatography-Bioautography and gas chromatography-mass spectrometry of antimicrobial leaf extracts from Philippine Piper betle L. against multidrug-resistant bacteria. Evidence-Based Complementary and Alternative Medicine 2016, 1-7.

Vikrama CP, S Murugesan, A Arivuchelvan, K Sukumar, A Arulmozhi, A Jagadeeswaran. 2018. GC-MS profiling of methanolic extract of Piper betle (Karpoori variety) leaf. Journal of Pharmacognosy and Phytochemistry 7(6), 2449-2452.

Villegas MV, Hartstein AI. 2003. Acinetobacter outbreaks, 1977–2000. Infection Control and Hospital Epidemiology 24(4), 284-295.

Wenzel RP, Fowler AA. 2006. Acute bronchitis. The New England Journal of Medicine 355(20), 2125-2130.

Wilson K. 2001. Preparation of genomic DNA from bacteria. Current Protocols in Molecular Biology 56, 2.4.1-2.4.5.

Sehr Syed, Umair Azmat, Shaista Ilyas, Arslan Fazal, Tanzeela Murmim, Fakhra Riaz, Maryam Khan, Saba Shamim.
Effectiveness of Piper betel leaf extracts against Acinetobacter species isolated from bronchitis and pharyngitis patients.
Int. J. Biosci. 17(5), 162-175, November 2020.
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