International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Enhanced yeast growth and bioethanol production using molasses and fermentation medium as substrates

By: Shafique Ahmed Rind, Fateh Muhammad Soomro, Agha Asad Noor, Atta Hussain Rind

Key Words: Yeast growth, molasses and fermentation medium, ethanol production.

Int. J. Biosci. 16(1), 455-464, January 2020.

DOI: http://dx.doi.org/10.12692/ijb/16.1.455-464

Certification: ijb 2020 0012 [Generate Certificate]

Abstract

The ethanol derived by the use of microorganisms during industrial fermentation process has been used as an alternate source of liquid energy and a substitute of high-cost petrol. Saccharomyces cerevisiae is best known microbial source due to its major impact on economic and social importance, rapid conversion of sugar, high growth and fermentation rate, high genetic stability and tolerance in stressful conditions. This work revealed that maximum growth of test strain on WL-nutrient broth, 0.8 ml inoculum size, 5.0 pH, and 32°C at 120 rpm after 72 hours. Glucose, sucrose; arginine, asparagine, glutamic acid; pantothenic acid, thiamine and riboflavin were the best organic sources for enhanced growth of test strains whereas calcium chloride, magnesium chloride, potassium chloride, ammonium chloride, ammonium sulphate, magnesium sulfate, manganese sulphate, potassium di-hydrogen phosphate, di-Potassium hydrogen phosphate, urea, peptone, yeast nitrogen base were the best chemicals for increased growth of test strain at their respective concentrations per 100ml. of WL-nutrient broth. The effect of alcohols showed that the test strain Cs tolerated higher concentration of ethanol as compared to the methanol and isopropanol. When studied the comparative growth, it was observed that the synthetic growth medium showed increased growth as compared to other media used. Ethanol production from clarified molasses and mixed with growth medium at different concentrations revealed 24.91% and 15.7% at sugar concentration 44 and 35.5% and sugar consumption 33.26 and 26.61% respectively.

| Views 16 |

| Views 16 |

Enhanced yeast growth and bioethanol production using molasses and fermentation medium as substrates

Aldiguer AS, Alfenore S, Cameleyer X, Goma G. 2004 .Synergistic temperature and ethanol effect on Saccharomyces cerevisiae dynamic behavior in ethanol bio-fuel production. Bioprocess Bio system Engineering volume 26, No. 4, 217-222.

Alegre RM, Rigo M, Jokes I. 2003. Ethanol fermentation of a diluted molasses medium by Saccharomyces cerevisiae immobilized on chrysotile. Brazalian Archieve of Biology and Technology volume 46, No. 4, 751-757.

AL-Sa’ady AJR. 2014.Optimization of invertase production from Saccharomyces cerevisiae by solid state fermentation. Current Research in Microbiology and Biotechnology volume 2, No. 3, 373-377.

Arifa T, Aftab M, Farasat T. bio-07. J. Applied 2010. Effect of cultural conditions on ethanol production by locally isolated Saccharomyces cerevisiae Pharmacology volume 3, No. 2, 72-78.

Arshad M, Khan ZM, Khalil-ur-Rehman, Shah FA, Rajoka MI. 2008. Optimization of process variables for minimization of byproduct formation during fermentation of blackstrap molasses to ethanol at industrial scale. Letters in Applied Microbiology volume 47, No. 5, pp. 410-414..

Balat M, Balat H, Oz C. 2008. Progress in bioethanol processing. Progress in Energy and Combustion Science volume 34, No. 5, 551-573.

Ballesteros M, Oliva JM, Negro MJ, Manzanares P, Ballesteros I. 2004. Ethanol from lignocellulosic materials by a simultaneous saccharification and fermentation process (SFS) with Kluyveromyces marxianus CECT 10875. Process Biochemistry volume 39, No. 12, 1843-1848.

Banat IM, Marchant R. 1995. Characterization and potential industrial applications of five novel, thermo-tolerant, fermentative yeast strains. World Journal of Microbiology and Biotechnology volume 11, No. 3, 304-306. https://doi.org/10.1007/BF00367104.

Bernardo O, Silva-Santisteban Y, Francisco. 2005. Agitation, aeration and shear stress as key factors in inulinase production by Kluyveromyces marxianus. Enzyme and Microbial Technology, volume 36, No. 5-6, 717-724.

Biofuels & Bioeconomy. 2018. The 8th International Conference on Biofuels, Bioenergy & Bioeconomy. Available from http://biofuels-bioeconomy.conferenceseries.com.

Byadgi SA, Kalburgi PB. 2016. Production of Bioethanol from Waste Newspaper. International Conference on Solid Waste Management, Procedia Environmental Sciences, volume 35, 555-562. https://doi.org/10.1016/j.proenv.07.040.

Duhan JS, Kumar A, Tanwar SK. 2013. Bioethanol production from starchy part of tuberous plant (potato) using Saccharomyces cerevisiae MTCC-170. African Journal of Microbiology Research volume 7, No. 46, 5253-5260.

Dung NTP, Tuong NH, Quang PH. 2012 .Testing of ethanol fermentation in sugarcane juice by thermotolerant yeasts. The 5th Satellite Seminar of JSPS-NRCT, Asian Core Program, 18-20.

Ghorbani F, Younesi H, Sri AE, Najafpour G. 2011 .Cane molasses fermentation for continuous ethanol production in an immobilized cells reactor by Saccharomyces cerevisiae. Renewable Energy volume 36, No. 2, 503-509.

Hahn-Hagerdal B, Gable M, Gorwa-Graslund MF, Liden G, Zacchi G. 2006. Bioethanol: The fuel of tomorrow from the residues of today. Trends in Biotechnology volume 24, No. 12, 549-556.

Hashimoto S, Ogura M, Aritomi K, Hoshida H, Nishizawa Y, Akada R. 2005. Isolation of auxotrophic mutants of diploid industrial yeast strains after UV mutagenesis. Applied and Environmental Microbiology volume 71, No. 1, 312-319,

http:// doi.org/10.1128/AEM.71.1.312-319.

Inan M, Chiruvolu V, Eskridge KM, Vlasuk GP, Dickerson K, Brow S. 1999 .Optimization of temperature, glycerol and pH conditions for fed-batch fermentation process for recombinant hookworm (Ancylostoma caninum) anticoagulant peptide (AcAP-5) production by Pichia pastoris. Enzyme and Microbiology Technology volume 24, No. 7, 438-445.

Inparuban K, Vasantharuba S, Balakumar S, Arasaratnam V. 2009. Optimization of culture conditions for Baker’s yeast cell mass production- a preliminary study. Journal of Science Eastern University Sri Lanka volume 6, No. 1, 34-45.

Janani K, Ketzi M, Megavathi S, Vinothkumar D, Babu RNG. 2013. Comparative Studies of Ethanol Production from different fruit wastes using Saccharomyces cerevisiae. International Journal of Innovative Research in Science, Journal Engineering and Technology volume 2, No. 12, pp. 7161-7167,

Kopsahelis N, Nisiotou A, Kourkoutas Y, Panas P, Nychas GJ, Kanellaki M. 2009. Molecular characterization and molasses fermentation performance of a wild yeast strain operating in an extremely wide temperature range. Bio resource Technology volume 100, No. 20, 4854-4862.

Landi C, Paciello L, de Alteriis E, Brambilla L, Parascandola P. 2001. Effect of auxotrophies on yeast performance in aerated fed-batch reactor. Biochemical Biophysical Research Communication volume 414, No. 3, 604-611.

Lin Y, Zhang W, Li C, Sakakibara K, Tanaka S, Kong H. 2012 .Factors affecting ethanol fermentation using Saccharomyces cerevisiae BY4742. Biomass and Bioenergy volume 47, 395-401. http://doi.org/10.1016/j.biombioe.2012.09.019.

Logothetis S, Walker G, Nerantzis ET. 2007. Effect of salt hyperosmotic stress on yeast cell viability. Proc Nat Sci Matica Srpska Novi Sad volume 113, 271-284.

Macedo IC, Seabra JEA, Silva JEAR. 2008. Greenhouse gases emissions in the production and use of ethanol from sugarcane in Brazil: the 2005/2006 averages and a prediction for 2020. Biomass and Bioenergy volume 32, No. 7, 582-595.

Manada P, Kathale N. 2015. Production of ethanol from mahua flower (Madhuca latifolia L.) using Saccharomyces cerevisiae- 3044 and study of parameters while fermentation. Journal of Research in Science and Technology, volume 1, No. 9, 6-10.

Mobini-Dehkordi M, Nahvi I, Zarkesh-Esfahani H, Ghaedi K, Tavassoli M, Akada R. 2011. Characterization of an interesting novel mutant strain of commercial Saccharomyces cerevisiae. Iranian Journal of Biotechnology, volume 9, No. 2, 109-114.

Mukherjee K, Banik AK. 2010. Effect of trace elements on biosorption of Hg2+ by Hg2+ tolerant. Saccharomyces cerevisiae A100. International. Journal of Pharmaceutical and Biosciences, volume 1, No. 2, 1-10.

Nielsen J, Larsson C, Maris VA, Pronk J. 2013. Metabolic engineering of yeast for production of fuels and chemicals. Current Opinion in Biotechnology, volume 24, No. 3, 398-404.

Noor AA, Dahot MU. 2008. Optimization studies of yeast feed for enhanced growth for ethanol production by shake flask method. Sindh University Research Journal (Sci. Ser.), Volume 40, No. 2, pp. 41-50.

Noor AA, Hameed A, Bhatti KP, Tunio SA. 2003 Bio-ethanol fermentation by the bioconversion of sugar from dates by Saccharomyces cerevisiae strains ASN-3 and HA-4. Biotechnology, volume 2, No. 1, 8-17.

Noor AA, Hameed A, Bhutto MA. 2005. Enhanced growth biotechnology of yeast for alcoholic fermentation. Biotechnology, volume 4, No. 1, 69-75.

Noor AA, Hameed A, Dahot MU. 2012. Yeast Feed (II) For Enhanced Growth for Ethanol Production. Sindh University Research Journal (Science Series), volume 44, No. 1, 97-104.

Noor AA, Hameed A, Ghumro PB. 1996 .The 96 hours multistep screening of sugar fermenting yeast Saccharomyces cerevisiae strain KA-1. J. Pure and Applied Science, volume 18, No. 1, 23-33.

Paciello AP, Parascandola P, Landi C. 2014. Auxotrophic Saccharomyces cerevisiae CEN.PK strains as new performers in ethanol production. Chemical Engineering Transactions, volume 38, 463-468.

Paciello L, Andrès I, Zueco J, Bianchi MM, de Alteriis E, Parascandola P. 2010. Expression of human interleukin-1b in Saccharomyces cerevisiae using PIR4 as fusion partner and production in aerated fedbatch reactor. Ann. Microbiol., Volume 60, 719-728.

Paciello L, Romano V, de Alteriis E, Zueco J, Parascandola P. 2009. Performance of the auxotrophic Saccharomyces cerevisiae BY4741 as host for human Interleukin-1β production in an aerated fed-batch reactor. Microbial Cell Factories, volume 8, 1-13.

Peña-Castro JM, del Moral S, Núñez-López L, Blanca E, Barrera-Figueroa, Amaya-Delgado L. 2017, Biotechnological Strategies to Improve Plant Biomass Quality for Bioethanol Production. BioMed Research International, volume 2017, 1-10.

Pérez-Torrado R. 2004. Estudio y mejora Del proceso de producción industrial de levaduras vínicas. PhD thesis, Universitat de València. Valencisa, Spain.

Periyasamy S. 2009. Production of Bio-ethanol from Sugar Molasses Using Saccharomyces cerevisiae. Modern and Applied Sciences, volume 3, No. 8, 32-37.

Phisalaphong M, Srirattana N, Tanthapanichakoon W. 2006. Mathematical modeling to investigate temperature effect on kinetic parameters of ethanol fermentation. Biochemical Engineering Journal, volume 28, No. 1, 36-43.

Phouong DNT, Huu TN, Xuan PH. 2014. Study on Ethanol fermentation conditions from molasses by thermo-tolerant yeasts. International Journal of Business and Applied Science, volume 1, No. 1, 13-22.

Reed G, Nagodawithana TW. 1988. Technology of Yeast Usage in Winemaking. American Journal of Enology and Viticulture, volume 39, No. 1, 83-90.

Rosma A, Ooi KI. 2006. Production of Candida utilizes biomass and intracellular protein content: Effect of agitation speed and aeration rate. Malaysian J. Microbiol. Volume 2, No. 2, pp. 15-18.

Samsuri M, Gozan M, Hermansyah H, Prasetya B, Nasikin M, Watanabe T. 2010. Ethanol production from bagasse with combination of cellulose cellubiase. In: simultaneous saccharification and fermentation (ssf) using white rot fungi pre-treatment. Journal of Chemical and Natural Resources Engineering, volume 3, 20-32.

Sanchez OJ, Carlos AC. 2008. Trends in biotechnological production of fuel ethanol from different feedstock. Biores. Technol., Volume 99, No. 13, pp. 5270-5295.

Saxena RC, Adhikari DK, Goyal HB. 2009. Biomass-based energy fuel through biochemical routes. A review. Renewable and Sustainable Energy Reviews, volume 13, 167-178.

Shah SFA. 2010. Enhanced production of ethanol from sugar cane molasses through thermotolerant Saccharomomy cescerevisiae cell. Ph. D. Thesis, Mehran University of Engineering and Technology, Jamshoro, Pakistan, 1-132.

Shaheen A. 2010. Production of ethanol from molasses using thermotolerant Kluyveromyces marxianus. Ph.D. thesis, Mehran University of Engineering and Technology, Jamshoro, Pakistan 1-187.

Stenberg K, Bollók MRéczey KGalbe M, Zacchi, G. 2000. Effect of substrate and cellulose concentration on simultaneous saccharification and fermentation steam-preheated soft wood for ethanol production. Biotechnology Bioengineering, volume 68, No. 2, 204-210.

Swain MR, Mishra J, Thatoi H. 2013. Bioethanol Production from Sweet Potato (Ipomoea batatas L.) Flour using Co-Culture of Trichoderma sp. and Saccharomyces cerevisiae in Solid-State Fermentation, Brazilian archives of Biology and Technology, volume 56, No. 2, 171-179.

Udhayaraja P, Narayanan JS. 2012. Optimization for production of bioethanol using sorghum stovar by Saccharomyces cerevisiae. International Journal of Research in Pure and Applied Microbiology, volume 2, No. 4, 64-67.

Walker GM. 2000 .Encyclopedia of Microbiology, Academic Press, volume 4, p. 939-953.

Ward OP, Singh A. 2006. Microbial technology for bioethanol production from agricultural waste. In: Ray RC Ed. Microbial Biotechnology in Agriculture and Aqua culture, vol. 1, Science Publishers, Enfield, New Hampshire, p. 517-557,.

Woodson M, Jablonowski CJ. 2008. An economic assessment of traditional and cellulosic ethanol technologies. Energy Sources, Part B., volume 3, 372-383.

http://doi:10.1080/15567240701232527.

WWI. 2006. Biofuels for transportation, global potential and implications for sustainable agriculture and energy in the 21st century, Management of Environmental Quality, volume 19, No. 1, p. 3-451. https://doi.org/10.1108/meq.2008.08319aae.005.

Wyman CE, Hinman ND. 1990. Ethanol: Fundamentals of ethanol production from renewable feed stocks and use as a transportation fuel. Applied Biochemistry and Biotechnology, Volume 24, No. 25, 735-753.

Xandé X, Archimède H, Gourdine JL, Anais C, Renaudeau D. 2010. Effects of the level of sugarcane molasses on growth and carcass performance of Caribbean growing pigs reared under a ground sugarcane stalks feeding system. Tropical Animal Health and Production  volume 42, No. 1, 13-20.

Shafique Ahmed Rind, Fateh Muhammad Soomro, Agha Asad Noor, Atta Hussain Rind.
Enhanced yeast growth and bioethanol production using molasses and fermentation medium as substrates.
Int. J. Biosci. 16(1), 455-464, January 2020.
https://innspub.net/ijb/enhanced-yeast-growth-bioethanol-production-using-molasses-fermentation-medium-substrates/
Copyright © 2020
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update