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Evaluating biotoxicity of cypermethrin towards non-targeted organisms by using phytotoxic, cytotoxic and antimicrobial bioassays

By: Maliha Ahmed, Naeem Ali

Key Words: Pesticides, Bio-toxicity, Pyrethroids, Phytotoxic, Antimicrobial

Int. J. Biosci. 16(4), 514-523, April 2020.


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Pesticides are inevitably used in the horticulture, aquaculture, and agriculture to prevent pests’ infestation and support systematic sustainability. A minute concentration of pesticides reaches the target community while rest of the volume either bulks-up in soil or reaches groundwater and aquatic systems through different channeling processes. Presence of pesticides is undesirable in these systems and may pose long term environmental hazards by interacting with the non-targeted communities in the vicinity. The current study was conducted to project the biotoxicity profile of an abundantly used pesticide ‘Cypermethrin’ by combining various toxicology parameters including Phytotoxicity, Cytotoxicity, Antibacterial and Antifungal activity assays. Evaluation of phytotoxicity of Cypermethrin showed a 50% reduction in radish seeds’ viability on exposure to 100ppm pesticide concentration. A significant decrease in the root and shoot length was recorded on 50 – 1000 ppm pesticide concentration. Cytotoxic profiling of Cypermethrin revealed detrimental effects of pesticide on the Artemia salina larvae, killing 50% of the population at a concentration of 11.161µg/L. Cypermethrin significantly inhibited growth of non-targeted bacterial species at a concentration of 10ppm and more, while showing drastic effect on the growth of Escherichia coli. Antifungal assay affirmed biotoxicity of pesticide towards fungal domain by reducing the growth of fungal species at a pesticide concentration of 10ppm and more with highest impact on the growth of Physarum polycephalum. By using a combination of novel and conventional model organisms, the current study reports biotoxicity of Cypermethrin which is an abundantly used pesticide in agricultural systems.

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Evaluating biotoxicity of cypermethrin towards non-targeted organisms by using phytotoxic, cytotoxic and antimicrobial bioassays

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Maliha Ahmed, Naeem Ali.
Evaluating biotoxicity of cypermethrin towards non-targeted organisms by using phytotoxic, cytotoxic and antimicrobial bioassays.
Int. J. Biosci. 16(4), 514-523, April 2020.
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