International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Evaluation of antibacterial activity of plant based silver nanoparticles in Synergism with Antibiotics

By: Faisal Rasheed Anjum, Sidra Anam, Sajjadur Rahman, Ashiq Ali, Ahsan Naveed, SadafNaz

Key Words: Silver nanoparticles, UV-vis spectrophotometer, Zeta potential, Minimal inhibitory concentration, Disk Diffusion Method.

Int. J. Biosci. 13(3), 250-261, September 2018.


Certification: ijb 2018 0005 [Generate Certificate]


Plant derived silver nanoparticles might be a possible replacement of antibiotics in order to treat multi drug resistant bacterial infections, hence could be the answer to antibiotic resistance. The current study deals with the biosynthesis of silver nanoparticles (AgNPs) and determination of their bactericidal activity against Staphylococcus aureus and Streptococcus pneumoniae. The reduction of silver nitrate was indicated by characteristic change of colour from light yellowish to deep brown and finally colloidal brown. Spectrophotometer analysis showed that maximum absorption was observed at 450 nm. Silver nanoparticles with crystalline structure having 19 nm domain size was observed. The surface charge of silver nanoparticles was found to be varying with pH of the environment. Minimal inhibitory concentration (MIC50 and MIC90) against Staphylococcus aureus was 2.66 & 5.31 µg/100 µl, while MIC50 and MIC90 against Streptococcus pneumoniae were found to be 5.31 & 1.06×101/100 µl, respectively. Disc Diffusion Method has revealed that biosynthesized AgNPs when used in synergism with antibiotics have significantly larger zone of inhibition as compared to AgNPs alone. Briefly, this research has provided a simple and environment friendly method for production of Ag particles as compared to complex chemical method. Moreover, green synthesized particles formulation showed a significant antibacterial activity against both test strains, whereas combinations of AgNPs and antibiotics have a better bactericidal effect.

| Views 21 |

Evaluation of antibacterial activity of plant based silver nanoparticles in Synergism with Antibiotics

Abubakar AS, Salisu IB, Chahal S. 2014. Green synthesis of nano silver particles using some selected plant species :comparative studies. Indian Journal of Applied Research 4, 5-8. Indian Journal ofApplied Research 4, 5-8.

Banerjee P, Mukhopahayay M, Das AP. 2014. Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis. Bio resources and Bioprocessing 1, 1-3.

Batarseh KI. 2004. Anomaly and correlation of killing in the therapeutic properties of silver (I) chelation with glutamic and tartaric acids. Journal of AntimicrobialChemotherapy 54, 546-548.

Dwivedi AD, Gopal K. 2010.Biosynthesis of silver and gold nanoparticles using Chenopodium album leaf extract. Colloids and Surfaces A: Physicochemical and Engineering Aspect 369, 27-33.

Gibbins B, Warner L. 2005. The role of antimicrobial silver nanotechnology. Medical    Device and Diagnostic Industry1, 1-2.

Gilberg K, Laouri M, Wade S, Isonaka S. 2003. Analysis of medication use patterns: apparent over use of antibiotics and underuse of prescription drugs for asthma, depression, and CHF. Journal of Manged Care Pharmacy 9,232-237.

Goswami SR, Sahareen T, Singh M, Kumar S. 2015. Role of biogenic silver nanoparticles in disruption of cell–cell adhesion in Staphylococcus aureus and Escherichia coli biofilm. Journal of Industrial and Engineering Chemistry 26, 73–80.

Haider A, Kang IK. 2015. Preparation of silver nanoparticles and their industrial and biomedical applications: a comprehensive review. Advances in Material  Science and Engineering.

Janardhanan R, Karuppaiah M, Hebalkar N, Rao TN. 2009. Synthesis and surface chemistry of nano silver particles. Polyhedron 28, 2522–2530.

Kumar L, Vemula LP, Ajayan ML, John G. 2008.Silver-nanoparticle-embedded antimicrobial paints based on vegetable oil. Nature Materials 7, 236-241.

Lalitha A, Subbaiya R, Ponmurugan V. 2013. Green synthesis of silver nanoparticles from leaf extract Azhadirachta indica and to study its anti-bacterial andantioxidant property. International journal of CurrentMicrobiology and Applied Science 2, 228–235.

Lok CN, Ho CM, Chen R, He QY, Yu WY, Sun V, Che CM. 2006. Proteomic analysis of the mode of antibacterial action of silver nanoparticles. Journal of Proteome Research 5, 916-924.

Madigan M, Martinko J. 2005. Brock Biology of Microorganisms, 11thed. Englewood     Cliffs, NJ: Prentice Hall.

Malik P, Shankar R, Malik V, Sharma V, Mukherjee TK. 2014. Green chemistry based benign routes for nanoparticle synthesis. Journal of Nanoparticles.

Manikandan R, Manikandan B, Raman T, Arunagirinathan K, Prabhu NM, Basu J,Per-umalM, Palanisamy S, Munusamy A. 2015. Biosynthesis of silver   nanoparticles using ethanolic petals extract of Rosa indica and characterization of its antibacterial, anticancer and anti-inflammatory activities. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 138, 120–129.

Manikandan V, Velmurugan P, Park JH, Chang WS, Park YJ, Jayanthi P, Cho P, Oh BT. 2017. Green synthesis of silver oxide nanoparticles and its                 antibacterial activity againstdental pathogens. 3 Biotech 7, 72.

Mazur M. 2004. Electrochemically prepsared silver nanoflakes and nanowires. Electrochemistry Communications 6, 400-403.

Mirzajani O, Khataee AR. 2010. UV/peroxydisulfate oxidation of CI Basic Blue 3: modeling of key factors by artificial neural network. Desalination 251, 64-69.

Qian Y, Yao J, Russel M, Chen K, Wang X. 2015. Characterization of green synthesizednano-formulation (ZnO-A. vera) and their antibacterial activity against pathogens. Environmental Toxicology and Pharmacology 39, 736–46.

Raffi M, Hussain F, Bhatti TM, Akhter JI, Hameed A, Hasan MM. 2008. Antibacterial  characterization of silver nanoparticles against E. coli ATCC-15224. Journal of Material Science and Technology 24, 192-196.

SarahIbrahim H, Ayad M, Fadhil A, Al-AniNK. 2014. Production of Ag nanoparticles using Aloe vera extract and its antimicrobial activity. Al-Nahrain Journal of Science 17,165–171.

Sathishkumar V, Sneha K, Won S, Kim VC, Yun V. 2009. Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver   particles and its bactericidal activity. Colloids and Surfaces B: Bio interfaces73, 332–338.

Savithramma N, Rao ML, Rukmini K, Suvarnalatha P. 2011. Antimicrobial activity of silver nanoparticles synthesized by using medicinal plants. International Journal of Chem Tech Research 3, 1394–1402.

Saxena RM, Zafar FT, Singh P. 2012. Green synthesis of silver nanoparticles using aqueous solution of Ficus benghalensis leaf extract and characterization of their antibacterial activity. Materials Letters 67, 91–94.

Shankar SS, Rai A, Ankamwar A, Singh A, Ahmad A, Sastry M. 2004. Biological synthesis of triangular gold nan prisms.Nature Materials 3, 482-488.

Sheehy K, Casey A, Murphy A, Chambers G. 2014. Antimicrobial properties of nanosilver: A cautionary approach to ionic interference. Journal of Colloid and Interface Science 443, 56-64.

Shockman GD, Barren JF. 1983. Structure, function, and assembly of cell walls of Gram-positive bacteria. Annual Reviews in Microbiology 37, 501-527.

Sui ZM, Chen X, Wang LY, Xu LM, Zhuang WC, Chai YC. 2006. Capping effect of CTAB on positively charged Ag nanoparticles. Physica E: Low-Dimensional Systems and Nanostructures 33, 308-14.

Vijayaraghavan K, Nalini SK, Prakash NU, Madhankumar D. 2012. Biomimetic synthesis of silvernanoparticles by aqueous extract of Syzygium aromaticum. Materials Letters 75, 33-35.

Zaman SB, Hussain MA, Nye R, Mehta V, Mamun KT, Hossain N. 2017. A review onantibi-oticresistance: Alarm bells are ringing. Cureus 9.

Faisal Rasheed Anjum, Sidra Anam, Sajjadur Rahman, Ashiq Ali, Ahsan Naveed, SadafNaz.
Evaluation of antibacterial activity of plant based silver nanoparticles in Synergism with Antibiotics.
Int. J. Biosci. 13(3), 250-261, September 2018.
Copyright © 2018
By Authors and International Network for
Natural Sciences (INNSPUB)
innspub logo
english language editing
    Publish Your Article
    Submit Your Article
Email Update