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Evaluation of the antimicrobial activities of different solvent extracts from the bark of Acacia Stenophylla A. CUNN. EX. BENTH.

By: Dawood Shah, Arshad Iqbal

Key Words: Antibacterial, Antifungal, Acacia stenophylla, Well diffusion.

Int. J. Biosci. 17(5), 184-194, November 2020.

DOI: http://dx.doi.org/10.12692/ijb/17.5.184-194

Certification: ijb 2020 0082 [Generate Certificate]

Abstract

The present study was conducted to evaluate the in-vitro antimicrobial activities of crude extracts derived from the bark of Acacia stenophylla by different organic solvents such as N-Hexane, Chloroform, Ethyl acetate, and N-Butanol. These solvent extracted samples were applied against three Gram-positive bacteria including Bacillus atrophaus, Bacillus subtilis, and Staphylococcus aureus, as well as four Gram-negative bacteria that comprised Klebsiella pneumonia, Pseudomonas aeruginosa, Salmonella typhi, and Escherichia coli. Additionally, the antimicrobial activities of these samples were also tested against one fungal strain i.e. Candida albicans. The antimicrobial potentials were measured by using the Well diffusion method in three different doses of 1, 2, and 3 mg, respectively. The antibiotic ciprofloxacin, anti-fungal clotrimazole, and DMSO were used as positive and negative control. Our results suggest that Salmonella typhi was a highly sensitive bacterial strain followed by Staphylococcus aureus showing 87.34% and 72.41% ZI, respectively.  Similarly, Bacillus atrophaus was found the most resistant bacterium tested. N-hexane was highly potent against Salmonella typhi that indicates that biologically active substances against S.typhi­ are non-polar. N-Hexane fraction showed maximum inhibitory potential against gram-positive bacteria as compared to other solvent fractions while crude methanol demonstrated maximum anti-microbial strength against Gram-positive, Gram-negative bacteria as well as fungal strain. This specifies the broad-spectrum antibacterial and antifungal potential of the crude methanol. Our study verified that crude methanol and its different solvent fractions extracted from the bark of A. stenophylla have promising antimicrobials that can be used against various bacterial and fungal diseases. Furthermore, a study is needed for the optimization of antimicrobials of the plant for commercial utilization.

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Evaluation of the antimicrobial activities of different solvent extracts from the bark of Acacia Stenophylla A. CUNN. EX. BENTH.

Ahmad B, Azam S, Bashir S, Ahmad J, Hussain F. 2011. Screening of Acacia modesta for haemagglutination, antibacterial, phytotoxic and Insecticidal activities. Journal of Medicinal Plants Research 5(14), 3090-3096.

https://doi.org/10.5897/JMPR.9001109

Akinsulire OR, Aibin I, Adenipekun T, Adelowotan T, Odugbemi T. 2007. In vitro antimicrobial activity of crude extracts from plants Bryophyllum pinnatum and Kalanchoe crenata. African Journal of Traditional, Complementary and Alternative Medicines 4(3), 338-344.

https://www.ajol.info/index.php/ajtcam/article/view/31227

Bakht J, Gohar N, Shafi M. 2014. In vitro antibacterial and antifungal activity of different solvent extracted samples of Alhagi maurorum. Pakistan Journal of Pharmaceutical Science 27(27), 1955-61.

https://applications.emro.who.int/imemrf/Pak_J_Pharm_Sci/Pak_J_Pharm_Sci_2014_27_6_1953_1959.pdf

Bakht J, Khan S, Shafi M. 2013. Antimicrobial potentials of fresh Allium cepa against gram positive and gram negative bacteria and fungi. Pakistan Journal of Botany 45(S1), 1-6.

https://www.pakbs.org/pjbot/PDFs/45(S1)/01.pdf

Berfad MA, Alnour TM. 2014. Phytochemical analysis and Antibacterial activity of the 5 different extract from the seagrasses Posidonia oceanica. Journal of Medicinal Plants Studies 2(4), 15-18.

https://www.plantsjournal.com/vol2Issue4/Issue_july_2014/1.1.pdf

Briskin DP. 2000. Medicinal plants and phytomedicines. Linking plant biochemistry and physiology to human health. Plant physiology, 124(2), 507-514.

https://doi.org/10.1104/pp.124.2.507

Cowan MM. 1999. Plant products as antimicrobial agents. Clinical Microbiology Reviews 12(4), 564-582.

https://doi.org/10.1128/CMR.12.4.564.

https://cmr.asm.org/content/12/4/564.short

Fazal H, Ahmad N, Abbasi BH, Abbass N. 2012. Selected medicinal plants used in herbal industries; their toxicity against pathogenic microoraganisms. Pakistan Journal of Botany 44(3), 1103-1109.

https://www.researchgate.net/profile/Hina_Fazal/publication/270763168_Selected_medicinal_plants_used_in_herbal_industries_their_toxicity_against_pathogenic_microoraganisms/links/54b4b6fc0cf2318f0f96e154.pdf

Iqbal A. 2015. Pharmacological studies of selected plants used traditionally in pain and inflammation, PhD thesis, Islamia Collage Peshawar). http://prr.hec.gov.pk/jspui/bitstream/123456789/12336/1/Arshad%20iqbal%20botany%202015%20icp%20peshwar%20prr.pdf

Khan A, Jan G, Khan A, Gul Jan F, Bahadur A, Danish M. 2017a. In vitro antioxidant and antimicrobial activities of Ephedra gerardiana (root and stem) crude extract and fractions. Evidence-Based Complementary and Alternative Medicine, 2017.

https://doi.org/10.1155/2017/4040254

Khan BM, Bakht J. 2016. “Antifungal, anti-yeast, anti-oxidant and HPLC analysis of different solvent extracted samples from Calamus aromaticus leaves.” Bangladesh Journal of Pharmacology 11(1), 91-100.

https://doi.org/10.3329/bjp.v11i1.23984

Khan BM, Bakht J, Shafi M. 2017b. Screening of leaves extracts from Calamus aromaticus for their antimicrobial activity by disc diffusion assay. Pakistan Journal of Pharmaceutical Sciences 30(3).

https://pubmed.ncbi.nlm.nih.gov/28653924/

Khan I, Ahmad H, AliN, Ahmad B, Tanoli H. 2013. Screening of Bunium bulbocastanum for antibacterial, antifungal, phytotoxic and haemagglutination activities. Pakistan Journal of Pharmaceutical Sciences 26(4), 787-791.

https://pubmed.ncbi.nlm.nih.gov/23811459/

Lim JK, Kang HJ, Kang SN, Lee BY. 2009. “Antioxidant and antimicrobial activities of various solvent fractions of fine ginseng root.” Food Science and Biotechnology 18, 513-518.

https://www.koreascience.or.kr/article/JAKO200917639069572.

Nabèrè O, Adama H, Samson G, Kiessoum K, Patrice Z, Roland MNT, Moussa C, Martin K, Jea MF. 2013. “Antibacterial and phytochemical studies of three Acanthaceae species used in Burkina Faso traditional medicine.” Journal of Applied Pharmaceutical Science 3(5), 49.

https://www.japsonline.com/admin/php/uploads/896_pdf.pdf

Nielsen TR, Kuete V, Jäger AK, Meyer JJM, Lall N. 2012. “Antimicrobial activity of selected South African medicinal plants.” BMC Complementary and Alternative Medicine 12(1), 74.

https://doi.org/10.1186/1472-6882-12-74

Njeru SN, Obonyo MA, Nyambati SO, Ngari S. M. 2015. “Antimicrobial and cytotoxicity properties of the crude extracts and fractions of Premna resinosa (Hochst.) Schauer (Compositae): Kenyan traditional medicinal plant.” BMC complementary and alternative medicine 15(1), 295.

https://doi.org/10.1186/s12906-015-0811-4.

Ogunbinu A, Okeniyi S, Flamini G, Cioni P, Ogunwande I, Babalola I. 2010. “Essential oil composition of Acacia nilotica Linn., and Acacia albida Delile (Leguminosae) from Nigeria.” Journal of Essential Oil Research, 22(6), 540-542.

https://doi.org/10.1080/10412905.2010.9700394

Onuegbu GC, Eze FI, Ayogu PC, Agbo MO, Ezema CG, Ezema BE. 2019. “Phytochemical and In Vitro Antimicrobial Properties of Premna hispida (Verbenaceae) Linn.” Journal of Pharmacy and Biological Sciences 5, 64-74.

https://doi.org/10.9790/3008-1405026474

Rahman MS, Rashid MA. 2008. “Antimicrobial activity and cytotoxicity of Eclipta prostrata.” Oriental Pharmacy and Experimental Medicine, 8, 47-52. https://doi.org/10.3742/OPEM.2008.8.1.047

Shinwari ZK. 2010. “Medicinal plants research in Pakistan.” Journal of medicinal plants research 4(3), 161-176.

https://doi.org/10.5897/JMPR.9000872.

Subedi A, Amatya MP, Shrestha TM, Mishra S. K, Pokhrel BM. 2012. “Antioxidant and antibacterial activity of methanolic extract of Machilus odoratissima.” Kathmandu University Journal of Science, engineering and technology 8(1), 73-80.

https://doi.org/10.3126/kuset.v8i1.6045

Subhan N. 2016. “Phytochemical and pharmacological investigations of Australian Acacia:.” An ethnomedicine-guided bioprospective approach. Australia: Phd thesis, Charles Sturt University.

https://scholar.google.com/scholar?cluster=2657923983858976507&hl=en&as_sdt=0,5#aHR0cHM6Ly9yZXNlYXJjaG91dHB1dC5jc3UuZWR1LmF1L3dzL3BvcnRhbGZpbGVzL3BvcnRhbC85MzE4NDA0Lzg4NDUzQEBAMQ

Subhan N, Burrows GE, Kerr PG, Obied HK. 2018. “Phytochemistry, Ethnomedicine, and Pharmacology of Acacia.” Studies in Natural Products Chemistry 57, 247-326.

https://doi.org/10.1016/B978-0-444-64057-4.00009-0.

Ullah N, Ahmad I, Ayaz S. 2014.In vitro antimicrobial and antiprotozoal activities, phytochemical screening and heavy metals toxicity of different parts of Ballotanigra. BioMed Research International, 2014.

https://doi.org/10.1155/2014/321803.

Wang H, Zhao M, Yang B, Jiang Y, Rao G. 2008. “Identification of polyphenols in tobacco leaf and their antioxidant and antimicrobial activities.” Food Chemistry 107(4), 1399-1406.

https://doi.org/10.1016/j.foodchem.2007.09.068

Dawood Shah, Arshad Iqbal.
Evaluation of the antimicrobial activities of different solvent extracts from the bark of Acacia Stenophylla A. CUNN. EX. BENTH..
Int. J. Biosci. 17(5), 184-194, November 2020.
https://innspub.net/ijb/evaluation-antimicrobial-activities-different-solvent-extracts-bark-acacia-stenophylla-cunn-ex-benth/
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