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Evaluation of the antimicrobial activity and cytotoxic effect of some uridine derivatives

By: Sarkar M. A. Kawsar, Mariam Islam, Sanjida Jesmin, Mohammad A. Manchur, Imtiaj Hasan, Sulatana Rajia

Key Words: Uridine, antibacterial, antifungal, toxicity and inhibition.

Int. J. Biosci. 12(6), 211-219, June 2018.


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Nucleoside analogues may represent good candidates for the discovery of new antimicrobial agents, therefore, a series of uridine derivatives (2-13) was assessed for their antibacterial and antifungal activities, and the relationship between the structure and activity of these molecules was outlined. The 2-bromobenzoylation of uridine derivatives was evaluated for in vitro antibacterial and antifungal screening studies against a number of human and plant pathogenic microorganisms by disc diffusion and food poisoned methods, respectively. From the antibacterial screening results, it was revealed that the test chemical 4 and 6 very significantly inhibited the growth of all Gram-positive and Gram-negative bacterial strains used. The inhibition of E. coli by 4 (14 mm), of S. typhiby 4 (15 mm), of B. subtilisby 6 (12 mm), of B. cereus by 6 (14 mm) were remarkable. However, the test chemical 10 inhibited the highest mycelial growth of Rhizopus nigricans (60.0%) against all examined fungal pathogens. For comparative studies, two standard antibiotics, Ampicillin and Nystatin, were also determined. In addition to that the toxicity results of brine shrimp lethality assay displayed the test chemicals 6, 7 and 8 highest levels of mortality (i.e., ~80% death) among all tested chemicals. Hence, uridine derivatives bearing various acyl substituents in the ribose moiety may represent good lead compounds for the future discovery of novel antibacterial and/or antifungal agents.

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Evaluation of the antimicrobial activity and cytotoxic effect of some uridine derivatives

WHO. 2008.The global burden of disease update. World Health Organization. Available at

Carlezon JWA, Mague SD, Parow AM, Stoll AL, Cohen BM, Renshaw PF. 2005. Antidepressant-like effects of uridine and omega-3 fatty acids are potentiated by combined treatment in rats. Biological Psychiatry, 57, 343-350.

Jonas DA, Elmadfa I, Engel KH, Heller KJ, Kozianowski G, Konig A, Muller D, Narbonne JF, Wackemagel W, Kleiner J. 2001. Safety considerations of DNA in food. Annals of Nutrition & Metabolism 45, 235-254.

Jordheim LP, Durantel D, Zoulim F, Dumontet C. 2013.Advances in the development of nucleoside and nucleotide analogues for cancer and viral diseases. Nature Reviews Drug Discovery 12, 447-464.

Minuesa G, Huber-Ruano I, Pastor-Anglada M, Koepsell H, Clotet B, Martinez-Picado J. 2011. Drug uptake transporters in antiretroviral therapy. PharmacologyTherapeutics132, 268-279.

Marçal PA, Sandra PT. 2016. Nucleoside transporter proteins as biomarkers of drug responsiveness and drugtargets. Molecular Pharmacology 6, 1-14.

Alan MS, Ronald MP. 1998. Antifungal Activity of 3′-Deoxyadenosine (Cordycepin). Antimicrobial Agents Chemotherapy42, 1424-1427.

PMCID: PMC105616

Richa S, Anudita B, Ramendra S. 2007. Synthesis and antimicrobial activity of some novel nucleoside analogues of adenosine and 1,3-dideazaadenosine. Bioorganic & Medicinal Chemistry Letters 17, 6239-44.

Gupta R, Paul S, Gupta AK, Kachroo PL, Bani S. 1997. Synthesis and biological activities of some 2-substituted phenyl-3-(3-alkyl/aryl-5,6-dihydro-s-triazolo [3,4-b][1,3,4]thiazolo-6-yl)-indoles. Indian Journal of Chemistry36, 707-710.

Kabir AKMS, Matin MM, Kawsar SMA. 1998. Antimicrobial activities of some selectively acylated uridine derivatives. Chittagong University Studies Part II: Science 22, 37-41.

Kabir AKMS, Kawsar SMA, Bhuiyan MMR, Rahman MS, Chowdhury ME. 2009. Antimicrobial screening studies of some derivatives of methyl α-D-glucopyranoside. Pakistan Journal of Scientific and IndustrialResearch52, 138-142.

Kabir AKMS, Dutta P, Anwar MN. 2004. Biological evaluation of some acylated derivatives of D-mannose. Pakistan Journal of Biological Sciences 7, 1730-1734.

Kawsar SMA, Hamida AA, Sheikh AU, Hossain MK, Shagir AC, Sanaullah AFM, Manchur MA, Imtiaj H, Ogawa Y, Fujii Y, Koide Y, Ozeki Y. 2015. Chemically modified uridine molecules incorporating acyl residues to enhance antibacterial and cytotoxic activities. International Journal of Organic Chemistry 5, 232-245.

Bauer AW, Kirby WMM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology 45, 439-476.

Miah MAT, Ahmed HU, Sharma NR, Ali A, Miah SA. 1990. Antifungal activity of some plant extracts. Bangladesh Journal of Botany 19, 05-10.

Grover RK, Moore JD.1962. Toximetric studies of fungicides against the brown rot organisms Sclerotiniaflucticola and S. laxa. Phytopathology 52, 876-880.

McLaughlin JL. 1991. In: K. Hostettmann (Ed.), Methods in Plant Biochemistry: Assays for bioactivity, ISBN: 0-124610161, London, Academic press1, 1-31 p.

Kabir AKMS, Matin MM, Kawsar SMA. 1998.  Synthesis and Antibacterial activities of some uridine derivatives. The Chittagong University Journal of Science 22, 13-18.

Kabir AKMS, Kawsar SMA, Bhuiyan MMR, Hossain S, Rahman MS. 2003. Biological evaluation of some uridine derivatives. The Chittagong University Journal of Science.27, 105-111.

Kawsar SMA, Faruk MO, Rahman MS, Fujii Y, Ozeki Y. 2014. Regioselective synthesis, characterization and antimicrobial activities of some new monosaccharide derivatives. Scientia Pharmaceutica 82, 1-20.

Kabir AKMS, Kawsar SMA, Bhuiyan MMR, Ferdous J, Rahman MS. 2013. Synthesis, characterization and microbial screening of some new methyl4,6-O-(4-methoxybenzylidene)-a-D’glucopyranoside derivatives. Journal of Bangladesh Academy of Sciences 37, 145-158.

Hunt WA. 1975. The effects of aliphatic alcohols on the biophysical and biochemical correlates of membrane function. Advances in Experimental Medicine and Biology56, 195-210.

Judge V, Narasimhan B, Ahuja M, Sriram D, Yogeeswari P, Clercq ED. Pannecouque C, Balzarini J. 2013. Synthesis, antimycobacterial, antiviral, antimicrobial activity and QSAR studies of N2-acyl isonicotinic acid hydrazide derivatives. Medicinal Chemistry9,53-76.

Kim YM, Farrah S. Baney RH. 2007. Structure–antimicrobial activity relationship for silanols, a new class of disinfectants, compared with alcohols and phenols. International Journal of Antimicrobial Agents 29, 217-222.

Sarkar M. A. Kawsar, Mariam Islam, Sanjida Jesmin, Mohammad A. Manchur, Imtiaj Hasan, Sulatana Rajia.
Evaluation of the antimicrobial activity and cytotoxic effect of some uridine derivatives.
Int. J. Biosci. 12(6), 211-219, June 2018.
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