International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Evaluation of CHROMagar Candida in the rapid identification of medically important species of Candida

By: Lucilyn D. Lahoylahoy, Bernadette C. Mendoza

Key Words: Candida, CHROMagar Candida, Germ tube test.

Int. J. Biosci. 11(1), 380-385, July 2017.


Certification: ijb 2017 0042 [Generate Certificate]


Providing a quick and effective method of identification of the important human pathogen Candida species is a vital step in delivering prompt and efficient disease treatment and determining antifungal susceptibility profile. This study was conducted with the aim of evaluating the use of CHROMagar Candida in the rapid presumptive taxonomical identification of C. albicans, C. parapsilosis, and C. krusei. The previous taxonomic classification of 188 Candida species were reconfirmed through the standard traditional methods of yeast purification, morphological and colonial characterization, and standard biochemical tests of sugar assimilation and fermentation. The traditional methods reconfirmed that all isolates belonged to the genus Candida: C. albicans (126), C. parapsilosis (41), and C. krusei (21). CHROMagar Candida (CA), a selective medium claimed to differentiate Candida species based on colony color, was compared to the germ tube test (GTT) in the presumptive identification of C. albicans. The use of GTT only confirmed 100 out of the 126 (79%) C. albicans isolates as compared to the 116 (92%) C. albicans verified using the CA medium. The use of CA gave a sensitivity of 92.1%, specificity of 98.4%, and accuracy of 94.1% compared with the 79.4% sensitivity, 70.5% specificity, and accuracy of 86.2% obtained for GTT. Overall, the better method to use for rapid and presumptive identification of C. albicans and C. parapsilosis is CHROMagar Candida.

| Views 148 |

| Views 148 |

Evaluation of CHROMagar Candida in the rapid identification of medically important species of Candida

Agu KC, Edet BE, Ada IC, Sunday AN, Chidi OB, Gladys AC, Uche EC, Uchenna OM, Chinedu OA. 2015. Isolation and characterization of microorganisms from oil polluted soil in Kwata, Awka South, Nigeria. American Journal of Current Microbiology 3, 46-59.

Anaissie EJ. 1992. Opportunistic mycoses in the immune-compromised host: experience at a cancer center and review. Clinical Infectious Diseases 14,


Aprianti D, Haryanto F, Purqon A, Khotimah SN, Viridi S. 2015. Study of budding yeast colony formation and its characterizations by using circular granular cell. Journal of Physics: Conference Series 694, 1-4.

Cameron SJS, Bolt F, Perdones-Montero A, Rickards T, Hardiman K, Abdolrasouli A, Burke A, Bodai Z, Karancsi T, Simon D, Schaffer R, Rebec M, Balog J, Takats Z. 2016. Rapid evaporative ionization mass spectrometry (REIMS) provides accurate direct from culture species identification within the genus Candida. Scientific Reports 6, 1-10.

Chin VK, Lee TY, Rusliza B, Chong, PP. 2016. Dissecting Candida albicans infection from the perspective of C. albicans virulence and omics approaches on host-pathogen interaction: A review. International Journal of Molecular Sciences 17, 1-17.

Deorukhkar SC, Saini S, Jadhav PA. 2012. Evaluation of different media for germ tube production of Candida albicans and Candida dubliniensis. International Journal of Biomedical and Advance Research 3(9), 704-707.

Hoppe JE, Frey P. 1999. Evaluation of six commercial tests and the germ-tube test for presumptive identification of Candida albicans. European Journal of Clinical Microbiology and Infectious Diseases 18, 188-191.

Kauffman CA, Fisher JF, Sobel JD, Newman CA. 2011. Candida urinary tract infections – diagnosis. Clinical Infectious Diseases 52(S6), 452-456.

Koneman EW, Schreehenberger PC, Allen SD, Winn WC, Janda WM. 1992. Colour atlas and textbook of diagnostic microbiology, 4th Ed. In: Winter R (Ed.). Philadelphia, USA: J.B. Lippincott Company.

Nemcova E, Cernochova M, Ruzicka F, Malisova B, Freiberger T, Nemec P. 2015. Rapid identification of medically important Candida isolates using high resolution melting analysis. Public Library of Science One 10(2), e0116940.

Odds FC. 1991. Sabouraud’s agar. Journal of Medical and Veterinary Mycology 29, 353-359.

Odds FC, Bernaerts R. 1994. CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species. Journal of Clinical Microbiology 32, 1923-1929.

Page BT, Kurtzman CP. 2005. Rapid identification of Candida species and other clinically important yeast species by flow cytometry. Journal of Clinical Microbiology. 43(9), 4507-4514.–4514.2005

Posteraro B, Torelli R, De Carolis E, Posteraro P, Sanguinetti M. 2011. Update on the laboratory diagnosis of invasive fungal infections. Mediterranean Journal of Hematology and Infectious Diseases 3(1), e2011002.

Raju SB, Rajappa S. 2011. Isolation and identification of Candida from the oral cavity. International Scholarly Research Network Dentistry 2011, 1-7.

Rippon JW. 1974. The pathogenic fungi and pathogenic actinomycetes. Medical Mycology. Philadelphia, USA: The W. B. Saunder Co.

Sumathi R, Devipriya S. 2016. Antibiogram of Candida albicans using Tabernaemontana divaricata leaf extracts by AWD assay. International Journal of Current Research in Biology and Medicine 1(2), 6-13.

Warren NG, Hazen KC. 1999. Candida, Cryptococcus, and other yeasts of medical importance. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, Eds. Manual of clinical microbiology. Washington DC, USA: American Society for Microbiology, 1184-1199.

Lucilyn D. Lahoylahoy, Bernadette C. Mendoza.
Evaluation of CHROMagar Candida in the rapid identification of medically important species of Candida.
Int. J. Biosci. 11(1), 380-385, July 2017.
Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
innspub logo
english language editing
    Publish Your Article
    Submit Your Article
Email Update