International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Extended spectrum beta lactamases producing non-lactose fermentative bacterial isolates causing blood stream infections in children

By: Anees Muhammad, Muhammad Jaseem Khan, Inam Ullah, Habib Ullah Khan, Ihteshamul Haq

Key Words: ESBL, BSIs, Children, Non-fermenter.

Int. J. Biosci. 15(2), 61-69, August 2019.

DOI: http://dx.doi.org/10.12692/ijb/15.2.61-69

Certification: ijb 2019 0067 [Generate Certificate]

Abstract

Blood stream infections (BSIs) are the important cause of morbidity and mortality in pediatrics. BSIs are usually caused by common gram positive and gram negative bacterial isolates but few uncommon bacteria may lead to BSIs in children significantly. The aim of the present study was to determine the drug resistance pattern of uncommon non-lactose fermenting gram negative bacterial isolates from blood specimen of children. A cross sectional study was conducted at tertiary care hospital, Peshawar from June to December 2018. Blood specimens were collected aseptically in BACTAMTM bottles and were processed in BACTEC 9120 according to the standard protocol. Antibiotics resistance profile was determined by using Kirby-Bauer Disc diffusion method. Bacterial isolates showed resistance to cephalosporin were further verified for extended spectrum beta lactamases (ESBL) by double disc diffusion method according to the clinical laboratory standards institute guidelines. Out of total, 20.6% were positive with significant growth in which 6.0% (07) isolates were non-lactose fermenter gram negative bacteria including Morgenella morganii (0.9%), Stenotrophomonas maltophila (2.7%), Acinetobacter baumannii (0.9) and Burkholderia cepacia (1.8%). Colistin/Polymixin B was found only effective antibiotics against Acinetobacter baumannii. Among recovered isolates, 42.9% were ESBL producer while 71.4 % were found multidrug resistant strains. It is concluded that non-fermenter bacterial isolates can contribute in blood stream infections significantly. ESBL producing by non-lactose fermenter bacterial isolates were identified with emerging MDR isolates to various antibiotics classes which is major concern in developing countries.

| Views 54 |

Extended spectrum beta lactamases producing non-lactose fermentative bacterial isolates causing blood stream infections in children

Adams-Sapper S, Sergeevna-Selezneva J, Tartof S, Raphael E, Diep BA, Perdreau-Remington F. 2012. Globally dispersed mobile drug-resistance genes in Gram-negative bacterial isolates from patients with bloodstream infections in a US urban general hospital. Journal of medical microbiology 61(Pt 7), 968.

Ahmed J, Jan AH, Nawaz G, Khan M. 2011. Epidemiology and antibiotic susceptibility of bacterial isolates from Northern Pakistan. African Journal of Microbiology Research 5(28), 4949-55.

http://dx.doi.org/10.5897/AJMR10.368

Anwar M, Ejaz H, Zafar A, Hamid H. 2016.  Phenotypic detection of metallo-beta-lactamases in carbapenem resistant Acinetobacter baumannii isolated from pediatric patients in Pakistan. Journals of Pathology.

http://dz.doi.org/10.1155/2016/8603964

Arega B, Woldeamanuel Y, Adane K, Sherif AA, Asrat D. 2018. Microbial spectrum and drug-resistance profile of isolates causing bloodstream infections in febrile cancer patients at a referral hospital in Addis Ababa, Ethiopia. Infection and drug resistance11, 1511.

Azargun R, Sadeghi MR, Barhaghi MHS, Kafil HS, Yeganeh F, Oskouee MA. 2018. The prevalence of plasmid-mediated quinolone resistance and ESBL-production in Enterobacteriaceae isolated from urinary tract infections. Infection and drug resistance (11), 1007.

Bajpai T, Pandey M. 2017. Eiological Landscape of Microoragnisms causing Blood Stream Infections: Commensal? or Pathogen? 6(14), 895-905.

http://dz.doi.org/10.20959/wjpr201714-999

Banik A, Bhat SH, Kumar A, Palit A, Snehaa K. 2018. Bloodstream infections and trends of antimicrobial sensitivity patterns at Port Blair. Journal of laboratory physicians 10(3), 332.

Barati M, Taher MT, Abasi R, Zadeh MM, Barati M, Shamshiri AR. 2009. Bacteriological profile and antimicrobial. Archives of Clinical Infectious Diseases 4(2), 87-95.

Bisharat N, Gorlachev T, Keness Y. 2012. 10-Years hospital experience in Pseudomonas stutzeri and literature. Open Infect Dis J. 6, 21-4.

Chen CY, Tsay W, Tang JL, Tien HF, Chen YC, Chang SC. 2010. Epidemiology of bloodstream infections in patients with haematological malignancies with and without neutropenia. Epidemiology & Infection 138(7), 1044-51.

http://dz.doi.org/10.1017/S095026880 9991208

Cho SY, Lee DG, Choi SM, Park C, Chun HS, Park YJ. 2015. Stenotrophomonas maltophilia bloodstream infection in patients with hematologic malignancies: a retrospective study and in vitro activities of antimicrobial combinations. BMC infectious diseases 15(1), 69.

Clinical Laboratory Standards Institute. 2014. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement. M100-S24. 13(1).

Dimple R, Jyoti R, Mahawal B, Ankit K. 2016. Prevalence of Gram negative bacteria causing neonatal septicemia in a tertiary care hospital of Dehradun, Uttarakhand, India. International Journal of Current Microbiology and Applied Science 5(1), 136-47.

Gupta I, Naskar P, Mitra G. 2016. Spectrum of bacterial infection and antimicrobial sensitivity pattern in neonatal septicemia in a peripheral tertiary care hospital in West Bengal. International Journal of Contemporary Medical Research 3, 2669-71.

Hannan A, Qamar MU, Usman M, Waheed KAI, Rauf K. 2013. Multidrug resistant microorganisms causing neonatal septicemia: In a tertiary care hospital Lahore, Pakistan. African Journal of Microbiology Research 7(19), 1896-902. http://dz.doi.org/10.5897/AJMR2012.2307

Kotgire S, Hatkar S. 2017. Aerobic bacteriological profile and its antimicrobial sensitivity pattern from blood culture specimens in a tertiary care hospital. Annals of Pathology and Laboratory Medicine 4(01).

Kwa A, Low J, Lim TP, Leow PC, Kurup A, Tam VH. 2008. Independent predictors for mortality in patients with positive Stenotrophomonas maltophilia cultures. Ann Acad Med Singapore 37(10), 826-30.

Laboratory Quality Assurance policy Manual. College of Physicians and Surgeons of Saskatchewan. Laboratory Quality Assurance Program. 2016.

Laing F, Ramotar K, Read RR, Alfieri N, Kureishi A, Henderson EA. 1995. Molecular epidemiology of Xanthomonas maltophilia colonization and infection in the hospital environment. Journal of clinical microbiology 33(3), 513-8.

Laxminarayan R, Matsoso P, Pant S, Brower C, Røttingen JA, Klugman K. 2016. Access to effective antimicrobials: a worldwide challenge. The Lancet 387(10014), 168-75.

Lugito H, Pratama N, Kurniawan A. 2016. A lethal case of Sphingomonas paucimobilis bacteremia in an immunocompromised patient. Case reports in infectious diseases 2.

McDonald LC, Banerjee SN, Jarvis WR, System NNIS. 1999. Seasonal variation of Acinetobacter infections: 1987–1996. Clinical infectious diseases 29(5), 1133-7.

Montefour K, Frieden J, Hurst S, Helmich C, Headley D, Martin M, 2008. Acinetobacter baumannii: an emerging multidrug-resistant pathogen in critical care. Critical care nurse 28(1), 15-25.

Munoz-Price LS, Zembower T, Penugonda S, Schreckenberger P, Lavin MA, Welbel S. 2010. Clinical outcomes of carbapenem-resistant Acinetobacter baumannii bloodstream infections: study of a 2-state monoclonal outbreak. Infection Control & Hospital Epidemiology 31(10), 1057-62. http://dz.doi.org/10.1086/656247

Naureen A, Saqib M, Muhammad F, Ahmad R, Muhammad G, Asi MN. 2010. Antimicrobial susceptibility of 41 Burkholderia mallei isolates from spontaneous outbreaks of equine glanders in Punjab, Pakistan. Journal of equine veterinary science 30(3), 134-40.

http://dz.doi.org/10.1016/j.jevs.2010.01.056

Naveed S, Zafar A, Javed H, Atif M, Abosalif KOAA, Ejaz H. 2018. Bacterial Spectrum and Antimicrobial Susceptibility Pattern in Septic Paediatric Patients. Pakistan Journal of Medical and Health Sciences 12(2), 845-8.

http://dz.doi.org/10.17582/journal.pjz/2017.49.6. 1997.2003

Naz SA, Tariq P. 2014. Prevalence of secondary infections with opportunistic bacteria in drug addicts suffering from tuberculosis. Int J Biol Biotechnol (Pak). 11, 363-7.

Ozdemir H, Kendirli T, Ergun H, Çiftçi E, Tapisiz A, Guriz H. 2011. Nosocomial infections due to Acinetobacter baumannii in a pediatric intensive care unit in Turkey. Turk J Pediatr 53(3), 255-60.

Peterside O, Pondei K, Akinbami FO. 2015.  Bacteriological profile and antibiotic susceptibility pattern of neonatal sepsis at a teaching hospital in Bayelsa state, Nigeria. Tropical medicine and health. 5(7).

Siebor E, Llanes C, Lafon I, Ogier-Desserrey A, Duez J, Pechinot A. 2007. Presumed pseudobacteremia outbreak resulting from contamination of proportional disinfectant dispenser. European Journal of Clinical Microbiology & Infectious Diseases 26(3), 195-8.

Tang HJ, Lai CC, Lin HL, Chao CM. 2014. Clinical manifestations of bacteremia caused by Aeromonas species in southern Taiwan. PLoS One. 9(3), e91642.

Tariq TM, Rasool E. 2016. Emerging trends of bloodstream infections: a six-year study at a paediatric tertiary care hospital in Kabul. J Coll Physicians Surg Pak JCPSP 26(11), 887-91.

Van Nguyen K, Do NTT, Chandna A, Nguyen TV, Van Pham C, Doan PM. 2013. Antibiotic use and resistance in emerging economies: a situation analysis for Viet Nam. BMC public health 13(1), 1158.

Wali R, Haque AU, Fadoo Z. 2012. Healthcare-associated infections among pediatric oncology patients in Pakistan: risk factors and outcome. The Journal of Infection in Developing Countries 6(05), 416-21.

Yemişen M, Balkan İİ, Salihoğlu A, Eşkazan AE, Mete B, Ar MC. 2016 The changing epidemiology of bloodstream infections and resistance in hematopoietic stem cell transplantation recipients. Turkish Journal of Hematology 33(3), 216.

Anees Muhammad, Muhammad Jaseem Khan, Inam Ullah, Habib Ullah Khan, Ihteshamul Haq.
Extended spectrum beta lactamases producing non-lactose fermentative bacterial isolates causing blood stream infections in children.
Int. J. Biosci. 15(2), 61-69, August 2019.
https://innspub.net/ijb/extended-spectrum-beta-lactamases-producing-non-lactose-fermentative-bacterial-isolates-causing-blood-stream-infections-children/
Copyright © 2019
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update