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The fertility of South Kalimantan buffalo spermatozoa after cold preservation and cryopreservation

By: Muhammad Rizal, Muhammad Riyadhi, Nursyam Andi Syarifuddin, Muhammad Thahir

Key Words: Sugar palm juice, Preservation, Cryopreservation, Artificial insemination, South Kalimantan buffalo.

Int. J. Biosci. 17(5), 85-95, November 2020.

DOI: http://dx.doi.org/10.12692/ijb/17.5.85-95

Certification: ijb 2020 0073 [Generate Certificate]

Abstract

Sugar palm juice can be an alternative extender of buffalo semen because it contains nutrients needed by spermatozoa during preservation. The objective of this study was to examine the fertility of South Kalimantan buffalo spermatozoa after the preservation and cryopreservation process.  Semen was collected by the artificial vagina. Fresh semen was divided into two tubes in equal volume and then diluted with sugar palm juice extender with a different composition. Semen in the first tube was diluted with 80% sugar palm juice + 20% egg yolk, and then stored in a refrigerator at 5ºC (chilled-semen) for three days. Semen in the second tube was diluted with 73% sugar palm juice + 20% egg yolk + 7% glycerol. Diluted-semen was loaded in a mini straw and then stored in a liquid nitrogen container (-196oC) for seven days. A total of 22 adult female buffaloes aged 3–5 years were selected as acceptors and estrous synchronization by PGF2α. Thirteen females were inseminated with chilled-semen, and nine females were inseminated with frozen semen The results of this study showed that motility of spermatozoa after preservation for three days was an average of 45.83% and 38.33% for frozen-thawed semen.  The percentage of pregnancy and birth for chilled-semen was 69.23% and 61.54%, higher than for frozen-thawed semen (66.67% and 55.55%), but there was no significant difference between treatment. It can be concluded that sugar palm juice is suitable as an alternative extender for South Kalimantan buffalo semen because it is able to maintain the quality and fertility of spermatozoa after preservation and cryopreservation.

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The fertility of South Kalimantan buffalo spermatozoa after cold preservation and cryopreservation

Abbas A, Andrabi SMH. 2002. Effect of different glycerol concentrations on motility before and after freezing, recovery rate, longevity and plasma membrane integrity of Nili-Ravi buffalo bull spermatozoa. Pakistan Veterinary Journal 22, 1-4.

http://pvj.com.pk/abstract/22_1/1.htm

Akhter S, Rakha BA, Ansari MS, Andrabi SMH, Ullah N. 2011. Storage of Nili-Ravi buffalo (Bubalus bubalis) semen in skim milk extender supplemented with ascorbic acid and α-tocopherol. Pakistan Journal of Zoology 43, 273-277.

https://docplayer.net/137400288-Storage-of-nili-ravi-buffalo-bubalus-bubalis-semen-in-skim-milk-extender-supplemented-with-ascorbic-acid-and-a-tocopherol.html

Almadaly EA, Tawfik FS, El-Kon II, Heleil BA, Fattouh EM. 2019. Effect of different cryoprotectents on the post-thaw sperm characteristics and in vivo fertility of buffalo (Bubalus bubalis) bull semen. Slovak Veterinary Research 56 (22), 541-551.

https://www.cabdirect.org/cabdirect/abstract/20193447175

Amin MR, Toelihere MR, Yusuf TL, Situmorang P. 1999. Pengaruh plasma semen sapi terhadap kualitas semen beku kerbau lumpur (Bubalus bubalis). Jurnal Ilmu Ternak dan Veteriner 4, 143-147.

http://dx.doi.org/10.14334/jitv.v4i3.151

Andrabi SMH, Ansari MS, Ullah N, Anwar M, Mehmood A, Akhter S. 2008. Duck egg yolk in extender improves the freezability of buffalo bull spermatozoa.  Animal Reproduction Science 104, 427-433.

https://doi.org/10.1016/j.anireprosci.2007.07.003

Ansari MS, Rakha BA, Andrabi SMH, Ullah N, Iqbal R, Holt WV, Akhter S. 2012. Glutathione supplemented tris-citric acid extender improves the postthaw quality and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa. Reproduction Biology 12, 271-6.

https://doi.org/10.1016/j.repbio.2010.10.001

Aviram M, Dornfield L, Rosenblatt M, Volkova N, Kaplan M, Coleman R. 2000. Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL, and platelet aggregation: studies in humans and in atherosclerotic apolipoprotein E-deficient mice. American Journal of Clinical Nutrition 71, 1062-1076.

http://dx.doi.org/10.1093/ajcn/71.5.1062

Aviram M, Rosenblatt M, Gaitani D, Nitecki S, Hoffman A, Dornfield L. 2004. Pomegranate juice consumption for 3 years by patients with carotidartery stenosis (CAS) reduces common carotid intima-media thickness (IMT), blood pressure and LDL oxidation. Clinical Nutrition 23, 423-433.

http://dx.doi.org/10.1016/j.clnu.2003.10.002

Barati F, Mahabady KM, Mohammadi GH. 2009. Cryopreservation of in situ cool stored buffalo (Bubalus bubalis) epididymal sperm. Iranian Journal of Veterinary Research 10, 339-345.

http://ijvr.shirazu.ac.ir/article_1718_354f16050fe0fb2226c57858cb3b6c76.pdf

Barile VL.  2005.  Improving reproductive efficiency in female buffaloes. Livestock Production Science 92, 183-194.

https://doi.org/10.1016/j.livprodsci.2004.06.014

Berber RCA, Madureira EH, Baruselli PS. 2002. Comparison of two ovsynch protocols (GnRH vs. LH) for fixed timed insemination in buffalo (Bubalus bubalis). Theriogenology 57, 1421-1430.

https://doi.org/10.1016/s0093-691x(02)00639-8

Brito LFC, Satrapa R, Marson EP, Kastelic JP. 2002. Efficacy of PGF2α to synchronize estrus in water buffalo cows (Bubalus bubalis) is dependent upon plasma progesterone concentration, corpus luteum size and ovarian follicular status before treatment. Animal Reproduction Science 73, 23-35.

https://doi.org/10.1016/S0378-4320(02)00124-0

Budiarto A, Ciptadi G, Hakim L, Putri AI. 2019. Reproductive performance and fertility index of swamp buffalo (Bubalus bubalis) in ngawi regency, east java. IOP Conf. Series: Journal of Physics: Conf. Series1146 (2019) 012024. Hlm 1-4.

https://doi.org/10.1088/1742-6596/1146/1/0212024

Daramola JO, Adekunle EO, Oke OE, Onagbesan OM, Oyewusi IK, Oyewusi JA. 2016. Effects of coconut (Cocos nucifera) water with or without egg-yolk on viability of cryopreserved buck spermatozoa. Animal Reproduction 13, 57-62.

http://dx.doi.org/10.21451/1984-3143-AR724

El-Sheshtawy RI, El-Nattat WS, Ali GAD. 2017. Cryopreservation of cattle semen using coconut water extender with different glycerol concentrations. Asian Pacific Journal of Reproduction 6, 279-282.

http://dx.doi.org/10.4103/2305-0500.217343

El-Sisy GA, Shahba MI, El-Sheshtawy RI. 2016. Frezeability of buffalo semen with TRIS extender enriched with disaccharides (trehalose or sucrose) and different glycerol concentrations. Asian Pacific Journal of Reproduction 5, 416-418.

https://doi.org/10.1016/j.apjr.2016.07.007

Gutiérrez-Añez JC, Palomares RA, Jiménez-Pineda JR, Camacho AR, Portillo-Martínez GE. 2018.  Pregnancy rate in water buffalo following fixed-time artificial insemination using new or used intravaginal devices with two progesterone concentrations. Tropical Animal Health Production 50, 629-634.

https://doi.org/10.1007/s11250-017-1479-1

Hafez ESE, Hafez B. 2000. Reproduction in Farm Animals 7th Edition. Lippincott Williams & Wilkins, Baltimore.

Hamid MA. 2018. Study on the effect of insemination time on pregnancy rate of Bangladeshi buffalo in intensive farming. SAARC Journal of Agriculture 16, 143-152.

https://doi.org/10.3329/sja.v16i2.40266

Hermansson U, Linde-Forsberg C. 2006. Freezing of stored, chilled dog spermatozoa. Theriogenology 65, 5845593.

https://doi.org/10.1016/j.theriogenology.2005.06.004

Hunter RHF. 1981. Physiology and Technology of Reproduction in Female Domestic Animals. Academic Press Limited, London.

Kulaksiz R, Ari UC, Daskin A, Uner AG. 2013. The effect of different glycerol concentrations on freezability of semen from agora, kilis, and saanen goats. Slovak Journal of Animal Science 46, 39-44

http://www.cvzv.sk/slju/13_2/1Kulaksiz.pdf

Kumar S, Sahni KL, Mohan G. 1992. Effects of different levels of glycerol and yolk on freezing and storage of buffalo semen in milk, Tris and sodium citrate buffers. Buffalo Journal 2, 151-156.

Leboeuf B, Restall B, Salamon S. 2000. Production and storage of goat semen for artificial insemination. Animal Reproduction Science 62, 113-141.

https://doi.org/10.1016/S0378-4320(00)00156-1

Lemma A. 2011. Effect of cryopreservation on sperm quality and fertility. In: M. Manafi (editor). 2011.  Artificial Insemination in Farm Animals. Accessed from

https://www.intechopen.com/books/artificial-insemination-in-farm-animals/effect-of-cryopreservation-on-sperm-quality-and-fertility

Mahmoud KGM, El-Sokary AAE, El-Roos MEAA, Ghaffar ADA, Nawito M. 2013. Sperm charactetistics in cryopreserved buffalo bull semen and field fertility. Iranian Journal of Applied Animal Science 3, 777-783.

http://ijas.iaurasht.ac.ir/article_513585.html

Martinez RRD, Hernandez J, Reyna L, Santos R. 2007. Intrauterine insemination with frozen-thawed semen in Creole goats, synchronized in oestrous during the non-breeding season. Researh Journal of Animal Science 1, 76-80.

http://medwelljournals.com/abstract/?doi=rjnasci.2007.76.80

Mazur P. 1984. Freezing of living cells: Mechanisms and implications. American Journal of Physiology 247, 125-142.

https://doi.org/10.1152/ajpcell.1984.247.3.C125

McKinnon AO. 1999. Breeding and its technology – now and the future. Accessed from www.harness.org.au/99wldcon/CONFEREN.HTM.

Morrell JM. 2006. Update on semen technologies for animal breeding. Reproduction Domestic Animal 41, 63-67.

https://doi.org/10.1111/j.1439-0531.2006.00621.x

Mughal DH, Ijaz A, Yousaf MS, Wadood F, Farooq U, Mahmood SA, Riaz A. 2018. Effect of osmotic pressure on spermatozoa characteristics of cryopreserved buffalo bull (Bubalus bubalis) semen. Journal of Applied Animal Research 46, 274-277.

https://doi.org/10.1080/09712119.2017.1295971

Naseer Z, Ahmad E, Singh J, Ahmad N. 2011. Fertility following CIDR based synchronization regimens in anoestrous Nili-Ravi buffaloes. Reproduction Domestic Animal 46, 814-817.

https://doi.org/10.1111/j.1439-0531.2010.01746.x

Neglia G, Gasparrini B, Palo RD, Rosa CD, Zicarelli L, Campanile G. 2003. Comparison of pregnancy rates with two estrus synchronization protocols in Italian Mediterranean Buffalo cows. Theriogenology 60, 125-133.

https://doi.org/10.1016/s0093-691x(02)01328-6

Parks JE, Graham JK. 1992. Effects of cryopreservation procedures on sperm membranes. Theriogenology 38, 209-222.

https://doi.org/10.1016/0093-691X(92)90231-F

Paul V, Prakash BS. 2005. Efficacy of the ovsynch protocol for synchronization of ovulation and fixed-time artificial insemination in Murrah buffaloes (Bubalus bubalis). Theriogenology 64, 1049-1060.

https://doi.org/10.1016/j.theriogenology.2001.02.004

Rizal M, Riyadhi M. 2016. Fertilitas semen kerbau rawa (Bubalus bubalis carabanensis) yang diencerkan dengan pengencer nira aren. Jurnal Veteriner 17, 457-467.

https://doi.org/10.19087/jveteriner.2016.17.3.457

Sansone G, Nastri MFJ, Fabbrocini A. 2000. Storage of buffalo (Bubalus bubalis) semen. Animal Reproduction Science 62, 55-76.

https://doi.org/10.1016/S0378-4320(00)00154-8

Seeram NP, Adams LS, Henning SM, Niu Y, Zhang Y, Nair MG, Heber D. 2005. In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, Ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. Journal of Nutritional Biochemistry 16, 360-367.

http://dx.doi.org/10.1016/j.jnutbio.2005.01.006

Sianturi RG, Purwantara B, Supriatna I, Amrozi, Situmorang P. 2012. Effect of glutathione and bovine seminal plasma in lactose extender on viability of swamp buffalo frozen semen. Jurnal Ilmu Ternak dan Veteriner 17, 169-178.

http://dx.doi.org/10.14334/jitv.v17i3.697

Singh A, Singh V, Narwade B, Mohanty T, Atreja S. 2012. Comparative quality assessment of buffalo (Bubalus bubalis) semen chilled (5°C) in egg yolk and soya milk-based extenders. Reproduction Domestic Animal 47, 596-600.

https://doi.org/10.1111/j.1439-0531.2011.01928.x

Singh P, Kumar D, Kumar P, Singh I, Yadav PS. 2013. Cryopreservation and quality assessment of buffalo bull semen collected from farmer’s doorstep. Agricultural Research 2, 148-52.

https://link.springer.com/article/10.1007/s40003-013-0056-8

Su L, Li X, Quan J, Yang S, Li Y, He X, Tang X. 2007. A comparison of the protective action of added egg yolks from five avian species to the cryopreservation of bull sperm. Animal Reproduction Science 104, 212–9.

https://doi.org/10.1016/j.anireprosci.2007.06.019

Stryer L. 1995. Biochemistry. WH Freeman and Company, New York.

Swelum AA, Mansour HA, Elsayed AA, Amer HA. 2011. Comparing ethylene glycol with glycerol for cryopreservation of buffalo bull semen in egg-yolk containing extender. Theriogenology 76, 833-842.

https://doi.org/10.1016/j.theriogenology.2011.04.015

Tarig AA, Wahid H, Rosnina Y, Yimer N, Goh YM, Baiee FH, Khumran AM, Salman H, Assi MA, Ebrahimi M. 2017. Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen. Veterinary World 10, 672-678.

http://dx.doi.org/10.14202/vetworld.2017.672-678

Tezcan F, Ozguven MG, Diken T, Ozcelik B, Erim FB. 2009. Antioxidant activity and total phenolic, organic acid and sugar content in commercial pomegranate juices. Food Chemistry 115, 873-877.

https://doi.org/10.1016/j.foodchem.2008.12.103

Wadood F, Aleem M, Ijaz A, Ahmad M, Yousaf M, Mughal H. 2016. Effect of extender osmolality and butylated hydoxy toluene supplementation on post-thaw quality and fertility of Nili Ravi buffalo bull (Bubalus bubalis) semen.  The Journal of Animal and Plant Science 26, 605-611.

https://pdfs.semanticscholar.org/9785/09633a22a4f46fc0cd32201d60fb953b7319.pdf

Warriach HM, Channa AA, Ahmad N. 2008. Effect of oestrus synchronization methods on oestrus behaviour, timing of ovulation and pregnancy rate during the breeding and low breeding seasons in Nili-Ravi buffaloes. Animal Reproduction Science 107, 62–67.

https://doi.org/10.1016/j.anireprosci.2007.06.007

Watson PF. 2000. The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science 60-61, 4815492.

https://doi.org/10.1016/S0378-4320(00)00099-3

Willoughby CE, Mazur P, Peter AT, Kritser JK. 1996. Osmotic tolerance limits and properties of murine spermatozoa. Biology of Reproduction 55, 715-727.

https://doi.org/10.1095/biolreprod55.3.715

Yulnawati, Gunawan M, Maheshwari H, Rizal M, Herdis, Boediono A. 2010. Quality of ejaculated and epididymal sperms spotted buffalo in dextrose supplemented extender. Hayati Journal of Biosciences 17, 27-30.

https://doi.org/10.4308/hjb.17.1.27

Yulnawati, Maheshwari H, Rizal M, Boediono A. 2013. Frozen-thawed epididymal sperm quality and the success rate of artificial insemination in spotted buffaloes (Bubalus bubalis carabanensis).  Buffalo Bulletin (Special Issue 2) 32, 494-497.

http://ibic.lib.ku.ac.th/e-Bulletin/IBBUSI201302039.pdf

Muhammad Rizal, Muhammad Riyadhi, Nursyam Andi Syarifuddin, Muhammad Thahir.
The fertility of South Kalimantan buffalo spermatozoa after cold preservation and cryopreservation.
Int. J. Biosci. 17(5), 85-95, November 2020.
https://innspub.net/ijb/fertility-south-kalimantan-buffalo-spermatozoa-cold-preservation-cryopreservation/
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