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Identification and characterization of at CNGC19 for its role in salt stress regulation in Arabidopsis thaliana

By: Sadaf Oranab, Bushra Munir, Khadim Hussain, Aftab Ahmad, Abdul Ghaffar

Key Words: Arabidopsis thaliana, Phylogenetic relationship, Salt and drought, Motif analysis, Knock out, Over expression lines.

Int. J. Biosci. 14(4), 378-386, April 2019.

DOI: http://dx.doi.org/10.12692/ijb/14.4.378-386

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Abstract

Cyclic nucleotide gated ion channels (CNGCs) in plants, animals and prokaryotes have very important role in signaling and development. Structural analysis of cyclic nucleotide gated ion channels showed that CNGC 4, 5, 6 and 9 had long untranslated introns, while CNGC 7, 8, 13 and 16 had fully translated exons and introns. The insertion was confirmed in STC13 on chromosome 3 through TAIL PCR and the expression of AtCNGC19 was determined by RT PCR, which was activated twice in control and about 5 times under 150mM NaCl. The expression of gene was significantly reduced in knock out lines. The over expression lines were generated for expressing AtCNGC19 under 35: S constitutive promoter. The calli showed salt tolerance at 150mM NaCl compared with control. AtCNGC19 showed significant enhancement under salt stress in microarray. CNGCs expression under abiotic stresses showed that CNGC 19 of group IVa was highly expressed in roots under salt stress after 6h. These results provide new evidences for role of AtCNGC19 undersalt stress regulation in Arabidopsis thaliana. The study for them explores new insights of AtCNGCs helpful for functional genomics of CNGCs in plants and for their roles during biotic and abiotic stress.

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Identification and characterization of at CNGC19 for its role in salt stress regulation in Arabidopsis thaliana

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Sadaf Oranab, Bushra Munir, Khadim Hussain, Aftab Ahmad, Abdul Ghaffar.
Identification and characterization of at CNGC19 for its role in salt stress regulation in Arabidopsis thaliana.
Int. J. Biosci. 14(4), 378-386, April 2019.
https://innspub.net/ijb/identification-characterization-cngc19-role-salt-stress-regulation-arabidopsis-thaliana/
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