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Identification of most effective sterilization protocol(S) for establishment of in vitro culture of endangered medicinal plant Skimmia laureola

By: Samina Siddique, Zishan Gul, Zafar Jamal, Asif ur Rehman

Key Words: Skemia laureola, Medicinal plant, Sterilization, Micro propagation

Int. J. Biosci. 12(2), 199-209, February 2018.

DOI: http://dx.doi.org/10.12692/ijb/12.2.199-209

Certification: ijb 2018 0266 [Generate Certificate]

Abstract

Skimmia laureola (DC) is an important endangered medicinal plant. The present research was conducted to investigate the effect of sterilizing procedure(s) for establishment of in vitro culture of Skimmia laureola. The explants were cultured on MS medium containing coconut water as growth promoter instead of any synthetic plant growth hormone. Mercuric chloride (HgCl2) at 0.5 %concentration for 30 seconds and 1 minute was found effective sterilization procedure when used alone or in combination with fungicide (Ridomil) where 100 % contamination was controlled with 100 % plants survived. Sodium hypochlorite (NaOCl) alone and with fungicide at both concentrations i.e 3% and 5% remained less effective, however, NaOCl + fungicide at 3% concentration for 1 minute showed 20% contamination with 80% survived explants. After 28 days the survived plantlets in HgCl2 treatment showed average 3.26 cm shoot length with average 5 number of leaves per explant. It has been concluded that HgCl2 can be used as sterilant for establishment of Skimmia laureola in vitro cultures and further work is needed for standardizing HgCl2 concentrations and time of exposure for sterilization of other endangered medicinal plants.

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Identification of most effective sterilization protocol(S) for establishment of in vitro culture of endangered medicinal plant Skimmia laureola

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Samina Siddique, Zishan Gul, Zafar Jamal, Asif ur Rehman.
Identification of most effective sterilization protocol(S) for establishment of in vitro culture of endangered medicinal plant Skimmia laureola.
Int. J. Biosci. 12(2), 199-209, February 2018.
https://innspub.net/ijb/identification-effective-sterilization-protocol-s-establishment-vitro-culture-endangered-medicinal-plant-skimmia-laureola/
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