International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

In vitro antibacterial evaluation of aqueous and methanol extracts of Tridax procumbens flower on selected pathogenic organisms

By: Enwa Felix Oghenemaro, Adjekuko Collins Ohwonigho, Mokogwu Azukaego Thomas Hughs, Efezino Merit

Key Words: Methanol extract, Aqueous extract, Gram-negative, Tridax procumbens, Antibacterial.

Int. J. Biosci. 18(6), 184-191, June 2021.

DOI: http://dx.doi.org/10.12692/ijb/18.6.184-191

Certification: ijb 2021 0249 [Generate Certificate]

Abstract

This study aimed at evaluating the antibacterial activity of the aqueous and methanol extracts of Tridax procumbens flowers against selected organisms. The powdered flowers of Tridax procumbens were extracted by cold maceration with water and methanol (70% v/v). The aqueous and methanolic extracts were subjected to antibacterial sensitivity test against selected organisms; Escherichia coli, Proteus vulgaris and Pseudomonas aeruginosa by determining the zone of inhibition using the agar well diffusion method. Results yielded varying MIC for each organism. The methanolic extract had an MIC value between 18.75mg/ml -150mg/ml and the aqueous extract had MIC values between 18.75mg/ml – 37.5mg/ml. In conclusion, both aqueous and methanolic extracts of Tridax procumbens flowers showed antibacterial activity against the selected isolates implying that the extracts of Tridax procumbens can serve as a good candidate for the antibacterial agent.

| Views 14 |

| Views 14 |

In vitro antibacterial evaluation of aqueous and methanol extracts of Tridax procumbens flower on selected pathogenic organisms

Aarti VZ, Saurabh B. 2010. Phytochemical investigation on Tridax Procumbens linn. Narsee Monjee Institute of Management Studies (NMiMS).

Adamu HM, Abayeh OJ, Agho MO, Abdullahi DL, Ubah A, Dukku HU. 2005. Antimicrobial activities of medicinal plants. Journal of Ethnopharmacology 99(1), 1-4

Ali M, Ravinder E, Ramachandram R. 2001. A new flavonoid from the aerial parts of Tridax procumbens.Fitoterapia 72(3), 313–5.

Anyamene CO, Ezeakdila JO. 2010. Antimicrobial activities of medicinal plants. Journal of applied science 13(1), 8940-8948.

Ikewuchi Jude C, Ikewuchi Catherine C, Igboh Ngozi M. 2009. Chemical Profile of Tridax procumbens Linn. Pakistan Journal of Nutrition 8, 548-550.

Choudhari MM, Maheshwari JK. 2009. Ethnobotany in south Asia, Middle East. Journal of scientific research 4, 144-146.

Dhanabalan R, Doss A, Jagadeeswari M, Ballachander S, Kezia E. 2008. In vitro phytochemical screening and antibacterial activity of aqueous and methanolic extract of Tridax procumbens against bovine mastitis isolated Staphylococcus aureus. Ethanobotanical leaflets 12, 1090-1095.

Mohale DS, Pokrna AR, Sanghani CV, Rasekar SR, Rathi AS, Rathod AS, Mehetre GR, Chandewar AV. 2014. Antimicrobial activity of methanolic extract of flowers of Tridax Procumbens.Indian Journal of Pharmacy and Pharmacology 1(1).

Durgacharan A, Bhagwat SG, Killedar, Rahul S, Adnaik. 2008 Antidiabetic activity of leaf extracts of Tridax procumbens. Indian Journal of Green Pharmacy 2, 126-127.

Gamboa-Leon Rubi, Vera-Ku Marina, Peraza-Sanchez Sergio R, Ku-Chulim Carlos, Horta-Baas Aurelio, Rosado-Vallado Miguel. 2014. Antileishmanial activity of a mixture of Tridax procumbens and Allium sativum in mice. Parasite 21, 15.

Hemant P, Sameer S, Balwant S, Khajja KJ, Jain GE. 2009. Evaluation of hypoglycemic and anti-hyperglycemic of Tridax procumbens. Fitoterapia 62, 307-313.

Ikewuchi CJ, Ikewuchi CC, Onwuka CF. 2010. Effect of aqueous extract of Tridax procumbens on plasma electrolysis of salt loaded rats. Pakistan Journal of Nutrition 9, 103-105.

Jamine R, Daisy P, Selvekumar BN. 2007. Against ESL-producing multi drug resistant anteric bacteria. Research journal of Microbiology 2, 369-374.

Kalaya A, Orasa P, Uraiwan P. 1997. Hypoglycemic activity of Tridax procumbens in rats.Thai Journal of Pharma Science 21, 211-221.

Kone WM, Atindehou KK, Terreaux C, Hosettman K, Traore D, Dosso M. 2004. Traditional medicine in North Cote d’ivoire; Screening of 50 medicinal plants for antibacterial activity. Journal. of Ethnopharmacol 93, 43-49.

Latha S, Kanaabiran K. 2006. Antimicrobial activity and phytochemicals of solanum trinobactum Limn. African Journal of Biotechnology 5, 23.

Manjamalia A, Sardar SSR, Guruvayooroppan C, Berlin GVM. 2010. Analysis of phytochemical constituents and antimicrobial activity of some medicinal plants. Global journal of Biotechnology and Biochemistry 5, 120-128.

Mir AS, Dar MA, Mir S, Ahmad SM, Chitale G. 2016. Analysis of Phytochemistry and antimicrobial activity of Tridax procumbens Linn. Journal of chemical science 7, 132.

Mir SA, Jan Z, Mir S, Dar AM, Chitale G. 2017. A Concise Review on Biological Activity of Tridax procumbens Linn. Organic Chemistry: Current Research 6, 177.

Nallella Sreeramulu, Suthari, Sateesh Ragan, Raju A, Vatsavaya S. 2013. Ethno-botanico-medicine for common human ailments in Nalgonda and Warangal districts of Telangana, Andhra Pradesh, India. Annals of Plant Sciences 2(7), 220–9.

Naveen PR, Vishwanthan S, Renuka DJ, Vijayashree N, Sweth VC. 2008. Priliminary phytochemical screening and antimicrobial activity of Somaneasaman. .Journal of Medicinal plant Research 2, 268-270.

Oghenemaro FE, Oliseloke CA, Itohan MI, Omoriode ME. 2019. Antibacterial evaluation of aqueous and methanol extracts of Tridax procumbens flower on selected gram negative and gram-positive bacteria. International Journal of Biosciences 15(3), 48-54.

Olandunmoye MK. 2006. Immunomodulatory effects of ethanolic extract of Tridax procumbens on swiss albino rats orogastrically dosed with Pseudomonas aeruginosa. Trends in medical research 1, 122-126.

Pathak AK, Saraf S, Dixit VK. 1991. Hair growth promoting activity of Tridax procumbens. Fitoterapia 62, 307–13.

Petchi R, Vijaya C, Parasuraman S. 2013. Anti-arthritic activity of ethanolic extract of Tridax procumbens (Linn.) in Sprague Dawley rats. Pharmacognosy Research 5(2), 113–7.

Prakash VD, Iravati K, Margaret I, Sattur PB. 1989. Phamacology and biochemical evaluation of Tridax procumbens in inflammation. Indian Journal of pharmacology 21, 17.

Rahman S, Ismail M, Mohammed N, Ali F, Chisthi AK, Imran. 2011. Evaluation of the stem bark of Pistacia integerrima stem for its antimicrobial and phytotoxic activities. African. Journal. Pharmacy, 5(8), 1170-1174.

Sandeep A, Srivastava RC. 2010. Antifungal property of Tridaxprocumbens against three phytopathogenic fungi. Arch Pharmacy Scientific Research 2, 258-263.

Saxena VK, Albert Sosanna. 2005. Β-Sitosterol-3-O-β-D-xylopyranoside from the flowers of Tridax procumbens Linn. Journal of Chemical Sciences 117(3), 263–6.

Suseela L, Sarsvarthy A, Brindha P. 2002. Pharmacognostic studies on Tridax procumbens L. (Asteraceae). Journal of phytological research 15(2), 141-7.

Taddei A, Rosas-Romero AJ. 2000. Bioactivity studies of extracts from Tridax procumbens. Phytomedcine 7, 235-238.

Bharathi V, Varalakshmi B, Gomathi S, Shanmuga Priya A,  Karpaga T. 2012. Antibacterial activity of Tridax procumbensLinn. International Journal of Pharma Sciences and Research. 3(4), 364-7.

Viluvanthan R, Kanchi SS, Thiruvengadam D. 2004. Effect of Tridax procumbens on liver antioxidant defence system during lipopolysacharide-induced hepatitis in D-galactosominesenitized rats. Molecular and Cellular Biochemistry 269, 131-136.

Hugo WB, Russell AD. 2007. Hugo and Russell’s Pharmaceutical Microbiology 7th edition. Blackwell science Ltd. 24, p 284.

Wani M, Pande S, More N. 2010. Callus induction studies in Tridax procumbens L. International Journal of Biotechnology Applications 2(1), 11–4.

WHO. 2014. Antimicrobial resistance fact sheet N°194. who. int. April 2014.

Enwa Felix Oghenemaro, Adjekuko Collins Ohwonigho, Mokogwu Azukaego Thomas Hughs, Efezino Merit.
In vitro antibacterial evaluation of aqueous and methanol extracts of Tridax procumbens flower on selected pathogenic organisms.
Int. J. Biosci. 18(6), 184-191, June 2021.
https://innspub.net/ijb/in-vitro-antibacterial-evaluation-of-aqueous-and-methanol-extracts-of-tridax-procumbens-flower-on-selected-pathogenic-organisms/
Copyright © 2021
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update