International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Influence of physicochemical conditions on the production of lipase by Psychrophilic Bacteria Isolated from Batura Glacier, Hunza Valley, Pakistan

By: Irfan Ullah, Mussarat Shaheen, Muhammad Rafiq, Muhammad Maqsood-ur-Rehman, Fariha Hasan

Key Words: Psychrophilic bacteria; lipase; physicochemical conditions; Batura glacier; Hunza valley.

Int. J. Biosci. 12(6), 1-11, June 2018.

DOI: http://dx.doi.org/10.12692/ijb/12.6.1-11

Certification: ijb 2018 0062 [Generate Certificate]

Abstract

Psychrophilic lipases have great biotechnological potential and are more preferable as compared to mesophilic or thermophilic lipases, due to their high activities at lower temperatures. In the present study, four bacterial isolates (HTB1, HTB2, LTB3, and HTB6), collected from different glaciers of Pakistan, screened for their lipase producing ability. On the basis of qualitative and quantitative analysis, out of four isolates, HTB2 isolate was selected for further production and optimization of lipase. Optimization of different culture conditions (incubation time, pH, temperature, carbon and nitrogen sources and various substrates) for the maximum production of lipase from HTB2isolate, were studied in shake flask fermentation. Maximum growth of HTB2isolate and lipase production (7.6 and 25.9U/mg), was observed at pH 9 and 10ºC, after 72hrs of incubation, respectively. Tween80, was found to be the best substrate for lipase production (20.8 U/mg), in liquid medium with pH 9, at low temperature (10ºC). Addition of glucose (19.4 U/mg) and casein (15.5U/mg) enhanced lipase production and were considered as good carbon and nitrogen sources for the production of lipase.

| Views 204 |

Influence of physicochemical conditions on the production of lipase by Psychrophilic Bacteria Isolated from Batura Glacier, Hunza Valley, Pakistan

Barbaro SE, Trevors JT, Inniss WE. 2001. Effects of low temperature, cold shock, and various carbon sources on esterase and lipase activities and exopolysaccharide production by a psychrotrophic Acinetobacter sp. Canadian Journal of Microbiology 47, 194-205.

https://doi.org/10.1139/w00-141

Beisson F, Tiss A, Rivière C, Verger R. 2000. Methods for lipase detection and assay: a critical review. European Journal of Lipid Science and Technology 102, 133-53.

https://doi.org/10.1002/(SICI)14389312(200002)102:2<133

Chander H, Batish VK, Sannabhadti SS, Srinivasan RA. 1980. Factors affecting lipase production in Aspergilluswentii. Journal of Food Science 45, 598-600.

https://doi.org/10.1111/j.1365-2621.1980.tb04109.x

Choo DW, Kurihara T, Suzuki T, Soda K, Esaki N. 1998. A cold-adapted lipase of an Alaskan psychrotroph, Pseudomonas sp. strain B11-1: gene cloning and enzyme purification and characterization. Applied and environmental Microbiology 64, 486-91.

Feller G, Narinx E, Arpigny JL, Aittaleb M, Baise E, Genicot S, Gerday C. 1996. Enzymes from psychrophilic organisms. Federation of European Microbiological Society’sMicrobiology Reviews 18, 189-202.

https://doi.org/10.1111/j.1574-6976.1996.tb00236.x

Grbavčić SŽ, Dimitrijević-Branković SI, Bezbradica DI, Šiler-Marinković SS, Knežević ZD. 2007. Effect of fermentation conditions on lipase production by Candida utilis. Journal of the Serbian Chemical Society 72, 757-65.

https://doi.org/10.2298/jsc0709757g

Gupta N, Sahai V, Gupta R. 2007. Alkaline lipase from a novel strain Burkholderia multivorans: Statistical medium optimization and production in a bioreactor. Process Biochemistry 30, 518-26.

https://doi.org/10.1016/j.procbio.2006.10.006

Hasan F, Shah AA, Hameed A. 2006. Industrial applications of microbial lipases. Enzyme and Microbial Technology 26, 235-51.

https://doi.org/10.1016/j.enzmictec.2005.10.016

Jaeger KE, Ransac S, Dijkstra BW, Colson C, van Heuvel M, Misset O. 1994. Bacterial lipases. Federation of European Microbiological Societies Microbiology Reviews 15, 29-63.

https://doi.org/10.1111/j.1574-6976.1994.tb00121.x

Kademi A, Danielle L, Ajain H. 2005. Lipases: Enzyme Technology 15, 297-318.

Kiran GS, Shanmughapriya S, Jayalakshmi J, Selvin J, Gandhimathi R, Sivaramakrishnan S, Arunkumar M, Thangavelu T, Natarajaseenivasan K. 2008. Optimization of extracellular psychrophilic alkaline lipase produced by marine Pseudomonas sp.(MSI057). Bioprocess and Biosystems Engineering 31, 483-92.

https://doi.org/10.1007/s00449-007-0186-0

Lesuisse E, Schanck K, Colson C. 1993. Purification and preliminary characterization of the extracellular lipase of Bacillus subtilis 168, an extremely basic pH‐tolerant enzyme.The Federation of European Biochemical Societies Journal 216, 155-60.

https://doi.org/10.1111/j.1432-1033.1993.tb18127.x

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265-75.

Mahler GF, Kok RG, Cordenons A, Hellingwerf KJ, Nudel BC. 2000.  Effects of carbon sources on extracellular lipase production and lipA transcription in Acinetobactercal coaceticus. Journal of Industrial Microbiology and Biotechnology 24, 25-30.

https://doi.org/10.1038/sj.jim.2900764

Margesin R, Spröer C, Schumann P, Schinner F. 2003. Pedobactercryoconitis sp. nov., a facultative psychrophile from alpine glacier cryoconite. International Journal of Systematic and Evolutionary Microbiology 53, 1291-6.

https://doi.org/10.1099/ijs.0.02436-0

Nashif SA, Nelson FE. 1953. The lipase of Pseudomonas fragi1 II. Factors affecting lipase production. Journal of Dairy Science 36, 471-80.

http://dx.doi.org/10.3168/jds.S00220302(53)91523-9

Park H, Lee KS, Chi YM, Jeong SW. 2005. Effects of methanol on the catalytic properties of porcine pancreatic lipase. Journal of Microbiology and Biotechnology 15, 296-301.

Pogaku P, Fan W, Suresh A, Zhong S, Srinivas P, Reddy SR, Ma C, Li P, Zhou K, Peng Z, Zhu M. 2010.Optimization of lipase production by Staphylococcus sp. Lp12. African Journal of Biotechnology 9, 882-6.

https://doi.org/10.5897/AJB09.1222

Pratuangdejkul J, Dharmsthiti S. 2000. Purification and characterization of lipase from psychrophilic Acinetobacter calcoaceticus LP009. Microbiological Research 155, 95-100.

Rajendran A, Palanisamy A, Thangavelu V. 2008. Evaluation of medium components by Plackett-Burman statistical design for lipase production by Candida rugosa and kinetic modeling. Chinese Journal of Biotechnology 24, 436-44.

https://doi.org/10.1016/S1872-2075(08)60024-2

Rathi P, Saxena RK, Gupta R. 2001. A novel alkaline lipase from Burkholderia cepacia for detergent formulation. Process Biochemistry 37, 187-92.

https://doi.org/10.1016/S0032-9592(01)00200-X

Ray N, Ray L, Srimani B, Chattopadhyay P. 1999. Isolation and identification of alkaline thermostable lipase producing microorganism, cultural conditions, nutritional requirements and some properties of crude enzyme. Indian Journal of Experimental Biology 37, 818-24.

Ruberto LA, Vazquez S, Lobalbo A, Mac Cormack WP. 2005. Psychrotolerant hydrocarbon-degrading Rhodococcus strains isolated from polluted Antarctic soils. Antarctic Science 17, 47-56.

https://doi.org/10.1017/S0954102005002415

Sebdani RM, Ardakani MR, Ghezelbash GR, Sadrinasab M. 2011. Phylogenic characterization of lipase producing Bacillus strains isolated from Persian Gulf sediments. Australian Journal of Basic and Applied Sciences 5, 121-6.

Senthilkumar R, Selvakumar G. 2008.Isolation and characterization of an extracellular lipase producing Bacillus sp. SS-1 from slaughterhouse soil. Advanced Biotechnology  6, 24-25.

Sugihara A, Tani T, Tominaga Y. Purification and characterization of a novel thermostable lipase from Bacillus sp. The Journal of Biochemistry 109, 211-6.

Thomson CA, Delaquis PJ, Mazza G. 1999. Detection and measurement of microbial lipase activity: a review. Critical Reviews in Food Science and Nutrition  39, 165-87.

https://doi.org/10.1080/10408399908500492

Van Dyke MI, Lee H, Trevors JT. 1991. Applications of microbial surfactants.Biotechnology Advances 9, 241-52.doi:

https://doi.org/10.1016/0734-9750(91)90006-H

Wang D, Xu Y, Shan T. 2008. Effects of oils and oil-related substrates on the synthetic activity of membrane-bound lipase from Rhizopus chinensis and optimization of the lipase fermentation media.Biochemical Engineering Journal 41, 30-7.

https://doi.org/10.1016/j.bej.2008.03.003

Wiseman A. 1995. Introduction to principles. Handbook of enzyme biotechnology. 3rd ed. Padstow, Cornwall, UK: Ellis Horwood Ltd. TJ Press, 3-8 p.

Zeng X, Xiao X, Wang P, Wang R. 2004. Screening and characterization of psychrotrophic, lipolytic bacteria from deep-sea sediments. Journal of Microbiology and Biotechnology 14, 952-8.

Irfan Ullah, Mussarat Shaheen, Muhammad Rafiq, Muhammad Maqsood-ur-Rehman, Fariha Hasan.
Influence of physicochemical conditions on the production of lipase by Psychrophilic Bacteria Isolated from Batura Glacier, Hunza Valley, Pakistan.
Int. J. Biosci. 12(6), 1-11, June 2018.
https://innspub.net/ijb/influence-physicochemical-conditions-production-lipase-psychrophilic-bacteria-isolated-batura-glacier-hunza-valley-pakistan/
Copyright © 2018
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update