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Investigation of haemato- and hepatotoxic effects of titanium dioxide nanoparticles in male sprague dawley rats

By: Adeel Khalid, Farhat Jabeen, Azhar Rasul, Salma Sultana, Kashif Zahoor, Muhammad Asif Latif

Key Words: Histological alteration, Epithelial lining, Karyolysis, Sinusoids, Lymphocytic.

Int. J. Biosci. 15(2), 182-193, August 2019.

DOI: http://dx.doi.org/10.12692/ijb/15.2.182-193

Certification: ijb 2019 0080 [Generate Certificate]

Abstract

This study investigated the toxic effect of TiO2 NPs on blood and liver tissues of male Sprague Dawley rats. For this purpose, 25 adult male Sprague Dawley rats of 200±5g weight were procured from the animal house of Government College University Faisalabad, Pakistan. Rats were acclimatized for 7 days before the start of study in ventilated cages at (25±2°C) with the approval of ethical committee of Government College University Faisalabad. These rats were randomly divided in to 5 groups. First control (C) with no treatment, 2nd placebo injected with normal saline (S) and three treated groups (G1, G2 and G3). The treated groups were intraperitoneally injected with TiO2 NPs @ 80 or 120 or 160 mg/kg BW of rats for 28 days on alternate day. Animal’s mortality, hematology and liver histology were evaluated. During the experiment no mortality was found while TiO2 NPs exposure showed highly significant pathological changes in haematological parameters and liver function test (p<0.05). The histological alterations were found severe in medium and high dose treated groups as compared to low dose while Control (C) and (S) saline treated groups showed normal histology. Treated groups showed different alterations such as vacuolation, damaging epithelial lining, karyolysis, sinusoids and neutrophilic and lymphocytic infiltration were highly significant in both G2 and G3 treated groups. This study explored the TiO2 -NPs caused toxicity in blood and hepatic tissues. It is concluded from present study that selected dose of titanium dioxide has negative impact on the health of living organisms.

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Investigation of haemato- and hepatotoxic effects of titanium dioxide nanoparticles in male sprague dawley rats

Abbasi-Oshaghi E, Mirzaei F, Pourjafar M. 2019. NLRP3 inflammasome oxidative stress and apoptosis induced in the intestine and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro study. International journal of nanomedicine 14, 1919.

https://doi.org/10.2147/IJN.SI92382

Abu-Dief EE, Khalil KM, Abdel-Aziz HO, Nor-Eldin EK, Ragab EE. 2015. Histological Effects of Titanium Dioxide Nanoparticles in Adult Male Albino Rat Liver and Possible Prophylactic Effects of Milk Thistle Seeds. Life Science Journal12(2), 115-123.

Alarifi S, Ali D, Al-Doaiss AA, Ali BA, Ahmed M, Al-Khedhairy AA. 2013. Histologic and apoptotic changes induced by titanium dioxide nanoparticles in the livers of rats. International Journal of Nanomedicine 8, 3937–3943.

http://dx.doi.org/10.2147/IJN.S47174

Allen R. 2016. The cytotoxic and genotoxic potential of titanium dioxide (TiO2) nanoparticles on human SH-SY5Y neuronal cells in vitro. The Plymouth Student Scientist 9, 5-28.

Amara S, Khemissi W, Mrad I, Rihane N, Ben Slama I, Mir LE, Sakly M. 2013. Effect of TiO2 nanoparticles on emotional behavior and biochemical parameters in adult Wistar rats. General Physiol. Biophysics 32(2), 229-234.

https://doi:10.4149/gpb_2013015

Azim SAA, Darwish HA, Rizk MZ, Ali SA, Kadry MO. 2015. Amelioration of titanium dioxide nanoparticles-induced liver injury in mice: possible role of some antioxidants. Experimental and Toxicological Pathology 67(4), 305-314.

http://dx.doi.org/10.1016/j.etp.2015.02.001

Bamidele FP, Ajibade AJ, Oyewo EB, Hannah AO. 2013. A study of some effects of aqueous extract of neem (Azadirachta indica) leaves on the lead acetate induced neurotoxicity in the superficial layers of superior colliculus of adult Wistar rats (Rattus norvegicus). British Journal of Pharmaceutical Research 3(2), 217–231.

Baldini E, Palmieri T, Pomarico E, Auböck G, Chergui M. 2019. Electron Dynamics in Anatase TiO2 Nanoparticles by Ultrafast Broadband Deep-Ultraviolet Spectroscopy. In EPJ Web of Conferences (205, p. 05017).

https://doi.org/10.1051/epjconf/201920505017

Cui Y, Chen X, Zhou Z, Lei Y, Ma M, Cao R, Sun T, Xu J, Huo M, Wen C, Che Y. 2014. Prenatal exposure to nanoparticulate titanium dioxide enhances depressive-like behaviors in adult rats. Chemosphere 96, 99-104.

http://dx.doi.org/10.1016/j.chemosphere.2013.07.051.

Dudefoi W, Moniz K, Allen-Vercoe E, Ropers M-H, Virginia K. 2017. Impact of food grade and nano- TiO2 particles on a human intestine community. Food and Chemical Toxicology 106, 242-249.

https://doi:10.1016/j.fct.2017.05.050

Elgrabli D, Beaudouin R, Jbilou N, Floriani M, Pery A, Rogerieux F. Lacroix G. 2015. Biodistribution and clearance of TiO(2) nanoparticles in rats after intravenous injection. PLoS One 10(4), e0124490.

https://doi:10.1371/journal.pone.0124490

Fadda LM, Ali HM, Mohamed AM, Hagar H. 2019. Prophylactic administration of carnosine and melatonin abates the incidence of apoptosis, inflammation, and DNA damage induced by titanium dioxide nanoparticles in rat livers. Environmental Science and Pollution Research, 1-9.

https://doi.org/10.1007/s11356-019-05059-4

Fadda LM, Hagar H, Mohamed AM, Ali HM. 2018. Quercetin and Idebenone Ameliorate Oxidative Stress, Inflammation, DNA damage, and Apoptosis Induced by Titanium Dioxide Nanoparticles in Rat Liver. Dose-Response, 16(4), 1559325818812188.

https://doi.org/10.1002/jbt.22040

Fakhar-e-Alam M, Abbas N, Imran M, Atif M. 2014. Apoptotic effect of TiO2 in HepG2 cellular model. Journal of Optoelectronics and Advanced Materials 16, 1481-1486.

Gate L, Disdier C, Cosnier F, Gagnaire F, Devoy J, Saba W, Mabondzo A. 2017. Biopersistence and translocation to extrapulmonary organs of titanium dioxide nanoparticles after subacute inhalation exposure to aerosol in adult and elderly rats. Toxicology letters 265, 61-69.

http://dx.doi.org/10.3390/ijms18122757

Grissa I, Guezguez S, Ezzi L, Chakroun S, Sallem A, Kerkeni E,  Haouas Z. 2016. The effect of titanium dioxide nanoparticles on neuroinflammation response in rat brain. Environmental Science and Pollution Research 23(20), 20205-20213.

https://doi.10.1007/s11356-016-7234-8

Gui S, Sang X, Zheng L, Ze Y, Zhao X, Sheng L, Wang L. 2013. Retracted article: Intragastric exposure to titanium dioxide nanoparticles induced nephrotoxicity in mice, assessed by physiological and gene expression modifications. Particle and fibre toxicology 10(1), 4.

https://doi.org/10.1186/s12989

Hong F, Yu X, Wu N, Yu-Qing, Zhang YQ. 2017. Progress of in vivo studies on the systemic toxicities induced by titanium dioxide nanoparticles. Toxicological Research 6, 115-133.

https://doi.10.1039/c6tx00338a

Hou J, Wang L, Wang C, Zhang S, Liu H, Li S, Wang X. 2019. Toxicity and mechanism of action of titanium dioxide nanoparticles in living organism.

Journal of Environmental Sciences (China) 75, 40-53.

https://doi.10.1016/j.jes.2018.06.010.

Hou X, Cai Y, Mushtaq M, Song X, Yang Q, Huang F, Wei Q. 2018. Deposition of TiO2 nanoparticles on porous polylactic acid fibrous substrates and its photocatalytic capability. Journal of nanoscience and nanotechnology 18(8), 5617-5623.

https://doi.org/10.1166/jnn.2018.15426

Jasim B, Thomas R, Mathew J, Radhakrishnan EK. 2017. Plant growth and diosegenin enhancement effect of silver nanoparticles in fenugreek (Trigonellafoenum graecum L) Saudi Pharmaceutical Journal 25 (3), 443-447.

https://doi.org/10.1016/j.jsps.2016.09.012

Khan I, Saeed K, Khan I. 2017. Nanoparticles: Properties, applications and toxicities. Arabian Journal of Chemistry.

https://doi.org/10.1016/j.arabjc.2017.05.011

Krawczyk M, Stanisz E. 2016. Ultrasound-assisted dispersive micro solid-phase extraction with nano-TiO2 as adsorbent for the determination of mercury species. Talanta 161, 384-391.

http://dx.doi.org/10.1016/j.talanta.2016.08.071

Lim JH, Sisco P, Mudalige TK, Sanchez- Pomales G, Howard PC, Linder SW. 2015. Detection and characterization of SiO2 and TiO2 nanostructure in dietary supplements. Journal of Agriculture and Food Chemistry 63, 3144-3152.

http://dx.doi.org/10.1021/acs.jafc.5b00392

Lu Z, Xie X, Zhou Y, Yu D, Deng Y, Ouyang J, Yang B, Luo D, Zhang D, Kuang H. 2018. Gestational exposure to titanium dioxide nanoparticles impairs the placentation through dysregulation of vascularization, proliferation and apoptosis in mice. International Journal of Nanomedicine 13, 777-789.

http://dx.doi.org/10.2147/IJN.S152400

Magdolenova Z, Collins A, Kumar A, Dhawan A, Stone V,  Dusinska M. 2014. Mechanisms of genotoxicity. A review of in vitro and in vivo studies with engineered nanoparticles. Nanotoxicology 8(3), 233-278.

https://doi.org/10.3109/17435390.2013.773464

Morgan A, Ibrahim MA, Galal MK, Ogaly HA, Abd-Elsalam RM. 2018. Innovative perception on using Tiron to modulate the hepatotoxicity induced by titanium dioxide nanoparticles in male rats. Biomedicine & Pharmacotherapy 103, 553-561. http://dx.doi.org/10.1016/j.biopha.2018.04.064

Noureen A, Jabeen F, Tabish TA, Ali M, Iqbal R, Yaqub S, Shakoor Chaudhry A. 2019. Histopathological changes and antioxidant responses in common carp (Cyprinus carpio) exposed to copper nanoparticles. Drug and chemical toxicology, 1-8.

http://dx.doi.org/10.1080/01480545.2019.1606233

Pandey RK, Prajapati VK. 2017. Molecular and immunological toxic effects of nanoparticles. International Journal of Biological Macromolecules 107, 1278-1293.

http://dx.doi.org/10.1016/j.ijbiomac.2017.09.110

Pierzchala K. 2010. Oxidative stress on human cells in the presence of nano-sized titanium dioxide. 2010. École polytechnique fédérale de Lausanne EPFL: Lausanne.

http://dx.doi.org/10.5075/epfl-thesis-4906

Pujalte I, Dieme D, Haddad S, Serventi AM, Bouchard M. 2017. Toxicokinetics of titanium dioxide (TiO2) nanoparticles after inhalation in rats. Toxicology letters 265, 77-85.

https://doi.org/10.1016/j.toxlet.2016.11.014

Rabhi S, Belkacemi H, Bououdina M, Kerrami  A, Brahem LA,  Sakher E. 2019. Effect of Ag doping of TiO2 nanoparticles on anatase-rutile phase transformation and excellent photodegradation of amlodipine besylate. Materials Letters 236, 640-643.  http://dx.doi.org/10.1016/j.matlet.2018.11.006

Samat MH, Ali AM M, Taib, MF M, Hassan, O.H, Yahya MZA. 2016. Hubbard U calculations on optical properties of 3d transition metal oxide TiO2. Results in physics 6, 891-896.

http://dx.doi.org/10.1016/j.rinp.2016.11.006

Shah SNA, Shah Z, Hussain M, Khan M. 2017. Hazardous effects of titanium dioxide nanoparticles in ecosystem. Bioinorganic chemistry and applications.

http://dx.doi.org/10.1155/2017/4101735

Shakeel M, Jabeen F, Iqbal R, Chaudhry AS, Zafar S, Ali M,  Asghar MS. 2018. Assessment of titanium dioxide nanoparticles (TiO2 NPs) Induced hepatotoxicity and ameliorative effects of Cinnamomum cassia in Sprague-Dawley rats. Biological Trace Element Research 182(1), 57-69.

https://doi.org.10.1007/s12011-017-1074-3

Shakeel M, Jabeen F, Qureshi NA, Fakhr-e-Alam M. 2016.Toxic Effects of Titanium Dioxide Nanoparticles and Titanium Dioxide Bulk Salt in the Liver and Blood of Male Sprague-Dawley Rats Assessed by Different Assays. Biological Trace Element Research 2(173), 405-426.

http://dx.doi.org/10.1007/s12011-016-0677-5

Sharma P, Singh R, Jan M. 2014. Dose-dependent effect of deltamethrin in testis, liver and kidney of Wistar rats. Toxicology International 21(2), 131–139.  http://dx.doi.org/10.4103/0971-6580.139789

Shinohara N, Oshima Y, Kobayashi T, Imatanaka N, Nakai M, Ichinose T, Gamo M. 2014. Dose-dependent clearance kinetics of intratracheally administered titanium dioxide nanoparticles in rat lung. Toxicology 325, 1-11.

https://doi.org/10.1016/j.tox.2014.08.003

Thomas S, Kumar Mishra P, Talegaonkar S. 2015. Ceramic nanoparticles fabrication methods and applications in drug delivery. Current pharmaceutical design 21(42), 6165-6188.

http://dx.doi.org/10.2174/1381612821666151027153246

Valentini X, Rugira P, Frau A, Tagliatti V, Conotte R, Laurent S, Nonclercq D. 2019. Hepatic and Renal Toxicity Induced by TiO2 Nanoparticles in Rats: A Morphological and Metabonomic Study. Journal of Toxicology.

http://dx.doi.org/10.1155/2019/5767012

Vance ME, Kuiken T, Vejerano EP, McGinnis SP, Hochella Jr  F, Rejeski D, Hull MS. 2015. Nanotechnology in the real world: Redeveloping the nanomaterial consumer products inventory. Beilstein journal of nanotechnology 6(1), 1769-1780.

http://dx.doi.org/10.3762/bjnano.6.181

Vasantharaja D, Ramalingam V, Aadinaath RG. 2015. Oral toxic exposure of titanium dioxide nanoparticles on serum biochemical changes in adult male Wistar rats. Nanomedicine Journal 2(1), 46–53.

Wang Y, Chen Z, Ba T, Pu J, Chen T, Song Y, Wang H. 2013. Susceptibility of young and adult rats to the oral toxicity of titanium dioxide nanoparticles. Small 9(910), 1742-1752.

https://doi.org/10.1002/smll.201201185

Younes NRB, Amara S, Mrad I, Ben-Slama I, Jeljeli M, Omri K, El GJ, El ML, Rhouma K, Abdelmelek H, Sakly M. 2015. Sub-acute toxicity of titanium dioxide (TiO2) nanoparticles in male rats: emotional behavior and pathophysiological examination. Environmental Science and Pollution Research 22(11), 8728–8737.

https://doi.org/10.1007/s11356-014-4002-5

Yu R, Fang X, Somasundaran P, Chandran K. 2015. Short-term effects of TiO2, CeO2, and ZnO nanoparticles on metabolic activities and gene expression of Nitrosomonas europaea. Chemosphere,  128, 207-215.

https://doi.org/10.1016/j.chemosphere.2015.02.002

Adeel Khalid, Farhat Jabeen, Azhar Rasul, Salma Sultana, Kashif Zahoor, Muhammad Asif Latif.
Investigation of haemato- and hepatotoxic effects of titanium dioxide nanoparticles in male sprague dawley rats.
Int. J. Biosci. 15(2), 182-193, August 2019.
https://innspub.net/ijb/investigation-haemato-hepatotoxic-effects-titanium-dioxide-nanoparticles-male-sprague-dawley-rats/
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