Isolation and Resistance Pattern of Bacteria from Infected Corneal Ulcer Patients in Human

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Research Paper 01/09/2021
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Isolation and Resistance Pattern of Bacteria from Infected Corneal Ulcer Patients in Human

Md. Anamul Haq, Mst. Mahbubay Habibah, Md. Rayhan Chowdhury, Md. Ariful Haque
Int. J. Biosci.19( 3), 83-89, September 2021.
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Abstract

A corneal ulcer is an infectious or more severely infective disease of the cornea, which affects the epithelial surface of the corneal stroma after trachoma. Corneal ulcers are the world’s second most important cause of blindness. This research was performed to determine the microbiological composition of corneal ulcers and the resistant pattern of bacteria collected from the patients of corneal ulcers from the department of ophthalmology at Rajshahi medical college hospital. The isolated bacteria were gram-negative. In Klebsiella selective agar, it showed a purple-magenta-colored colony, as well as in ESBL selective agar; the isolated bacteria showed a purple or blue-colored colony. Thus the isolate has been identified as Klebsiella pneumoniae by selective agar test. Eight different antibiotics were used to evaluate the isolate for antibiotic sensitivity using the disc diffusion method. The isolated bacteria showed resistance to three of the antibiotics, namely ampicillin, vancomycin, and bacitracin, and showed susceptibility to five such as gentamicin, amikacin, chloramphenicol, imipenem, levofloxacin. The isolated bacteria exhibited 37.5% resistance and 62.5% sensitivity against those eight antibiotics. According to the findings of this study, Klebsiella pneumoniae can be detected in corneal ulcers, and mentioned antibiotics can be used to treat these bacteria.

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Abubakar UM, Lawan A,  Muhammad I. 2018. Clinical pattern and antibiotic sensitivity of bacterial corneal ulcers in Kano, Northern Nigeria. Annals of African Medicine 17(3), 151. https://doi.org/10.4103/aam.aam_59_17

Amatya R, Shrestha S, Khanal B, Gurung R, Poudyal N, Bhattacharya SK,  Badu BP. 2012. Etiological agents of corneal ulcer: five years prospective study in eastern Nepal 14(3), 219-222. Nepal Medical College Journal : NMCJ.

Bauer AW, Kirby WM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology 45, 149-158. https://doi.org/10.1093/ajcp/45.4_ts.493

Bergey’s Manual® of Systematic Bacteriology. 2005. In Bergey’s Manual® of Systematic Bacteriology. https://doi.org/10.1007/0-387-29298-5

Bhadange Y, Sharma S, Das S, Sahu SK. 2013. Role of liquid culture media in the laboratory diagnosis of microbial keratitis. American Journal of Ophthalmology 156(4), 745-751. https://doi.org/10.1016/j.ajo.2013.05.035

Bruce SK, Schick DG, Tanaka L, Jimenez EM, Montgomerie JZ. 1981. Selective medium for isolation of Klebsiella pneumoniae. Journal of Clinical Microbiology 13(6), 1114-1116. https://doi.org/10.1128/jcm.13.6.1114-1116.1981

Buck JD, Cleverdon RC. 1960. THE SPREAD PLATE AS A METHOD FOR THE ENUMERATION OF MARINE BACTERIA,. Limnology and Oceanography 5(1), 78-80. https://doi.org/10.4319/lo.1960.5.1.0078

Cao J, Yang Y, Yang W, Wu R, Xiao X, Yuan J, Xing Y, Tan X. 2014. Prevalence of infectious keratitis in Central China. BMC Ophthalmology 14(1), 1-6. https://doi.org/10.1186/1471-2415-14-43

Collier SA, Gronostaj MP, MacGurn AK, Cope, JR, Awsumb KL., Yoder JS, Beach MJ, Centers for Disease Control and Prevention (CDC). 2014. Estimated burden of keratitis–United States, 2010. MMWR. Morbidity and Mortality Weekly Report.

Flonta M, Almas A. 2011. Phenotypic methods for detection of beta-lactamasemediated resistance in E. Coli and Klebsiella pneumoniae. Therapeutics 15(1), Pharmacology and Clinical Toxicology.

International Organisation for Standardisation. 2002. Microbiology of food and animal feeding stuffs — horizontal method for the detection of Salmonella spp. International Organisation for Standardisation, ISO 6579:2002 (E). Geneva: International Organization for Standardization (ISO).

Jain DN, Kamble VA. 2017. Molecular characterization of corneal ulcers causing Staphylococcus aureus. International Journal of Research in Medical Sciences 5(4), 1419-1424. https://doi.org/10.18203/2320-6012.ijrms20171238

Jondle CN, Gupta K, Mishra BB, Sharma J. 2018. Klebsiella pneumoniae infection of murine neutrophils impairs their efferocytic clearance by modulating cell death machinery 14(10), PLoS Pathogens. https://doi.org/10.1371/journal.ppat.1007338

Kaliamurthy J, Kalavathy CM, Parmar P, Nelson Jesudasan CA, Thomas PA. 2013. Spectrum of bacterial keratitis at a tertiary eye care centre in India. BioMed Research International. https://doi.org/10.1155/2013/181564

Katara RS, Patel ND, Sinha M. 2013. A clinical microbiological study of corneal ulcer patients at Western Gujarat, India 399-403. Acta Medica Iranica.

Kato GJ, Steinberg MH, Gladwin MT. 2017. Intravascular hemolysis and the pathophysiology of sickle cell disease 127(3), 750-760. In Journal of Clinical Investigation. https://doi.org/10.1172/JCI89741

Leck AK, Kalavathy CM, Essuman V, Jesudasan CAN, Johnson GJ, Thomas PA, Hagan M, Kaliamurthy J, Ackuaku E, John, M, Newman MJ, Codjoe FS, Opintan JA. 2002. Aetiology of suppurative corneal ulcers in Ghana and south India, and epidemiology of fungal keratitis 86(11), 1211-1215. In British Journal of Ophthalmology. https://doi.org/10.1136/bjo.86.11.1211

Lenchenko E, Blumenkrants D, Sachivkina N, Shadrova N, Ibragimova A. 2020). Morphological and adhesive properties of Klebsiella pneumoniae biofilms. Veterinary World. https://doi.org/10.14202/vetworld.2020.197-200

McDonnell PJ, Nobe J, Gauderman WJ, Lee P, Aiello A., Trousdale, M. 1992. Community care of corneal ulcers. American Journal of Ophthalmology 114(5), 531-538. https://doi.org/10.1016/S0002-9394(14)74479-4

Palioura S, Henry CR, Amescua G, Alfonso E. C. 2016. Role of steroids in the treatment of bacterial keratitis 10, 179. In Clinical Ophthalmology. https://doi.org/10.2147/OPTH.S80411

Rahman MS, Rana MS, Hosen MB, Habib M. A, Nur A, Kaly MK, Matin MN, Nasirujjaman, K, Rahman MM. 2021. Isolation and characterization of bacteria from fermented cooked-rice 1-6. Biologia. https://doi.org/10.1007/s11756-021-00753-6

Sanders ER. 2012. Aseptic laboratory techniques: Plating methods. Journal of Visualized Experiments. https://doi.org/10.3791/3064

Shah A, Sachdev A, Coggon D, Hossain P. (2011). Geographic variations in microbial keratitis: An analysis of the peer-reviewed literature. In British Journal of Ophthalmology 95(6), 762-767. https://doi.org/10.1136/bjo.2009.169607

Tanure MAG, Cohen EJ, Sudesh S, Rapuano C. J, Laibson PR. 2000. Spectrum of fungal keratitis at Wills Eye Hospital, Philadelphia, Pennsylvania 19(3), 307-312. Cornea. https://doi.org/10.1097/00003226-200005000-00010

Ting DSJ, Ho CS, Deshmukh R, Said DG, Dua HS. 2021. Infectious keratitis: an update on epidemiology, causative microorganisms, risk factors, and antimicrobial resistance 35:1084–1101. In Eye (Basingstoke). https://doi.org/10.1038/s41433-020-01339-3

Toriyama K, Suzuki T, Shiraishi A. 2018. Characteristics of Infectious Keratitis in Old and Very Old Patients. Journal of Ocular Pharmacology and Therapeutics 34(8), 565-569. https://doi.org/10.1089/jop.2018.0028

Vajpayee RB, Dada T, Saxena R, Vajpayee M, Taylor HR, Venkatesh P, Sharma N. 2000. Study of the first contact management profile of cases of infectious keratitis: A hospital-based study 19(1), 52-56. Cornea. https://doi.org/10.1097/00003226-200001000-00011

Verma R, Khanna P, Prinja S, Rajput M, Arora V. 2011. The national programme for control of blindness in India 4(1), 1. In Australasian Medical Journal. https://doi.org/10.4066/AMJ.2011.505

Whitcher JP, Srinivasan M, Upadhyay MP. 2001. Corneal blindness: A global perspective 79, 214-221. Bulletin of the World Health Organization. https://doi.org/10.1590/S0042-96862001000300009

Zare M, Torbati PM, Asadi-Amoli F, Talebnejad M. Parvizi M, Nasiri Z, Gharebaghi R, Heidary F. 2019. Microbiological Profile of Corneal Ulcers at a Tertiary Referral Center 8(1), 16-21. Medical Hypothesis, Discovery & Innovation Ophthalmology Journal.