International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Isolation and characterization of potential probiotic bacteria from sahiwal cow gut

By: Shahid Zaman, Madeeha Gohar, Misbah Tubassam, Maria Qubtia, Shakira Ghazanfar, Muhammad Imran

Key Words: Probiotic, Lactic acid bacteria, Sahiwal cow, 16S rDNA gene.

Int. J. Biosci. 15(3), 261-271, September 2019.

DOI: http://dx.doi.org/10.12692/ijb/15.3.261-271

Certification: ijb 2019 0138 [Generate Certificate]

Abstract

Farm animal productivity depends upon many factors including gut microbiome. It is well established that dysbiosis in the gut microbiome leads to compromised productivity and metabolic disorder. Microbial species with probiotic potential can be isolated from dung samples. From twelve cow dung samples total of nineteen bacterial strains were isolated on two different growth media (M17 and TSA). Only four gram-positive strains were isolated and conformed through 16S rDNA sequencing. Fully characterized strains QAULL04 (KP256013) and QAUEM01 (KP273582) were checked for their probiotic potential. QAULL04 and QAUEM01 has shown maximum mimic gut survival of 31.70% and 3.35% after two hours respectively while it reduces to 26.72% and 0% after 24 hours respectively. Cholesterol assimilation potential of QAULL04 was high (64.44%) in comparison with QAUEM01 (50%) while cell hydrophobicity of QAUEM01 was high (21.10%) than QAULL04 (4.712%). Cumulatively QAULL04 has shown better results for its probiotic application in animal feed.

| Views 32 |

Isolation and characterization of potential probiotic bacteria from sahiwal cow gut

Bai S. 2012. Cellulase Production by Bacillus subtilis isolated from Cow Dung. Archives of Applied Science Research scholars research library 4(1), 269-279.

Cebra JJ. 1999. e role of mucosal microbiota in the development and maintenance of the mucosal immune system. New York, Academic Press.

Cotta M. 1988. Amylolytic activity of selected species of ruminal bacteria. Applied and Environmental Microbiology 54(3), 772-776.

Cotter PD, Hill C. 2003. Surviving the acid test: responses of gram-positive bacteria to low pH. Microbiology and Molecular Biology Reviews 67(3), 429-453.

https://doi.org/10.1128/mmbr.67.3.429-453.2003 

Cullimore R. 2008. Practical atlas for bacterial identification.

https://doi.org/10.1201/97814200879.87 

Del Piano M. 2006. Probiotics: from research to consumer. Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver 38 Suppl 2, S248-255.

https://doi.org/10.1016/s1590-8658(07)60004-8 

Driessen FM, de Boer R.  1989. Fermented milks with selected intestinal bacteria: a healthy trend in new products.Netherlands Milk Dairy Journal 43, 367–382.

Dunne C. 2001. In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings.The American Journal of Clinical

Nutritin 73(2 Suppl), 386S-392S.

https://doi.org/10.1093/ajcn/73.2.38.6s 

Elmer GW. 1999.  Survival of   Saccharomyces. boulardii  in the rat gastrointestinal tract and effects of dietary fiber.Microbial Ecology in Health and Disease 11, 29–34.

https://doi.org/10.1080/089106099435.899 

Fukushima M, Nakano M.  1996. Effects of a mixture of organisms, Lactobacillus acidophilus or Streptococcus faecalis on cholesterol metabolism in rats fed on a fat- and cholesterol-enriched diet. British Journal of Nutrition 76(6), 857-867.

https://doi.org/10.1079/bjn19960.092 

Fuller R. 1989. Probiotics in man and animals. The Journal of applied bacteriology 66(5), 365-378.

https://doi.org/10.1111/j.1365-2672.1989.tb051.05.x 

Guarner F, Malagelada JR. 2003. Gut flora in health and disease.The Lancet 361(9356), 512-519.

https://doi.org/10.1016/s0140-6736(03)124.89-0 

Hofmann AF, Mysels KJ. 1992. Bile-Acid Solubility and Precipitation Invitro and Invivo – the Role of Conjugation, Ph, and Ca2+ Ions.  Journal of Lipid Research 33(5), 617-626.

Holzapfel WH. 1998. Overview of gut flora and probiotics.International Journal of Food Microbiology 41(2), 85-101.

https://doi.org/10.1016/s0168-1605(98)00.044-0 

Johnsen H, Krause K.  2014. Cellulase activity screening using pure carboxymethylcellulose: application to soluble cellulolytic samples and to plant tissue prints. International journal of molecular sciences 15(1), 830-838. 

https://doi.org/10.3390/ijms15010830

Klaenhammer TR. 2012. The impact of probiotics and prebiotics on the immune system. Nature Reviews Immunology 12(10), 728-734.

https://doi.org/10.1038/nri33.12 

Klaver FAM, Vandermeer R. 1993. The Assumed Assimilation of Cholesterol by Lactobacilli and Bifidobacterium-Bifidum Is Due to Their Bile Salt-Deconjugating Activity. Applied and Environmental Microbiology 59(4), 1120-1124.

Klein G. 1998. Taxonomy and physiology of probiotic lactic acid bacteria.International Journal of Food Microbiology41(2), 103-125.

https://doi.org/10.1016/s0168-1605(98)00.049-x 

Krepsky N. 2003. Cell surface hydrophobicity and slime production of Staphylococcus epidermidis Brazilian isolates. Current microbiology 46(4), 0280-0286.

https://doi.org/10.1007/s00284-002-386.8-5 

Kullen MJ, Klaenhammer TR. 1999. Identification of the pH‐inducible,  proton‐translocating F1F0‐ATPase (atpBEFHAGDC) operon of Lactobacillus acidophilus by differential display: gene structure, cloning and characterization. Molecular microbiology 33(6), 1152-1161.

https://doi.org/10.1046/j.1365-2958.1999.0155.7.x 

Liong MT, Shah NP. 2005. Acid and bile tolerance and cholesterol removal ability of lactobacilli strains. Journal of Dairy Science 88(1), 55-66.

https://doi.org/10.3168/jds.s0022-0302(05)72.662-x 

Lorca GL. 2002. Characterization of the protein-synthesis dependent adaptive acid tolerance response in Lactobacillus acidophilus. Journal of molecular microbiology and biotechnology 4(6), 525-532.

Metchnikoff E. 1907. The prolongation of life. Optimisticstudies. London, UK, William Heinemann.

Mohamed SA. 2010. Production of cellulase in Low Cost Medium by Bacillus Subtilis KO strain. World Applied Sciences Journal 8(1), 35-42.

Musa H. 2009. The potential benefits of probiotics in animal production and health. Journal of Animaland Veterinary Advances 8(2), 313-321.

Nurmi E, Rantala M. 1973. New aspects of Salmonella infection in broiler production. Nature 241(5386), 210-211.

https://doi.org/10.1038/241210.a0 

Parvez S. 2006. Probiotics and their fermented food products are beneficial for health. Journal of Applied Microbiology 100(6), 1171-1185.

https://doi.org/10.1111/j.1365-2672.2006.02963.x 

Pereira DI, Gibson GR. 2002. Cholesterol assimilation by lactic acid bacteria and bifidobacteria isolated from the human gut.Applied and Environmental Microbiology 68(9), 4689-4693.

https://doi.org/10.1128/aem.68.9.4689-4693.20.02 

Pereira DIA. 2003. An in vitro study of the probiotic potential of a bile-salt-hydrolyzing Lactobacillus fermentum strain, and determination of its cholesterol-lowering properties.Applied and Environmental Microbiology 69(8), 4743-4752.

https://doi.org/10.1128/aem.69.8.4743-4752.2.003 

Razin S. 1980. Phospholipid and Cholesterol Uptake by Mycoplasma Cells and Membranes. Biochimica Et Biophysica Acta(BBA) – Biomembranes 598(3), 628-640.

https://doi.org/10.1016/0005-2736(80)900.42-5 

Roos S. 2005. Lactobacillus gastricus sp nov., Lactobacillus antri sp nov., Lactobacillus kalixensis sp nov and Lactobacillus ultunensis sp nov., isolated from human stomach mucosa. International Journal of Systematic and Evolutionary Microbiology 55, 77-82.

https://doi.org/10.1099/ijs.0.6308.3-0 

Senesi S. 2001. Molecular characterization and identification of Bacillus clausii Strains marketed for use in oral bacteriotherapy. Applied and Environmental Microbiology 67(2), 834-839.

https://doi.org/10.1128/aem.67.2.834-839.2001 

Strohlein. 2003. Back to nature.Live yeasts in feed for dairy cows.  DMZ,Lebensm. Ind.Milcheirtsh 124, 68-71.

Takahashi M. 2004. The effect of probiotic treatment with Clostridium butyricum on enterohemorrhagic Escherichia coli O157:H7 infection in mice. Federation of European Microbiological Societies Immunology and Medical Microbiology41(3), 219-226.

https://doi.org/10.1016/j.femsim.2004.03.010 

Tamai Y. 1996. Effects of milk fermented by culturing with various lactic acid bacteria and a yeast on serum cholesterol level in rats. Journal of Fermentation and Bioengineering 81(2), 181-182.

https://doi.org/10.1016/0922-338x(96)87.601-x 

Vacheethasanee K. 1998. Bacterial surface properties of clinically isolated Staphylococcus epidermidis strains determine adhesion on polyethylene. Journal of Biomedical Materials Research: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and the Australian Society for Biomaterials 42(3), 425-432.

https://doi.org/10.1002/(sici)1097-46.36(19981205)42:3<425::aid-jbm12>3.3.co;2-j 

Wilson K. 2001. Preparation of genomic DNA from bacteria. Current protocols in molecular biology 56(1), 2.4. 1-2.4. 5.

https://doi.org/10.1002/0471142727.mb0204.s56

Shahid Zaman, Madeeha Gohar, Misbah Tubassam, Maria Qubtia, Shakira Ghazanfar, Muhammad Imran.
Isolation and characterization of potential probiotic bacteria from sahiwal cow gut.
Int. J. Biosci. 15(3), 261-271, September 2019.
https://innspub.net/ijb/isolation-characterization-potential-probiotic-bacteria-sahiwal-cow-gut/
Copyright © 2019
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update