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Isolation, identification and characterization of Rhizobium from lentil root nodule

By: Most. Farhana Begom, Md. Giush Uddin Ahmed, Rebeka Sultana, Md. Zahurul Islam

Key Words: Rhizobium, Root nodules, Red gram, nitrogen fixation, Biofertilizer.

Int. J. Biosci. 18(4), 22-28, April 2021.

DOI: http://dx.doi.org/10.12692/ijb/18.4.22-28

Certification: ijb 2021 0199 [Generate Certificate]

Abstract

The use of some microorganisms are capable of fixing atmospheric nitrogen can reduce chemical (nitrogen) contamination in the soil. The main purpose of this research paper is to isolate Rhizobium as a nitrogen fixing bacteria from root nodules of lentil (Lens culinaris) and its identification, characterization and hence the production of biofertilizer. In culture initiation ssurvival rates were higher using 2-3% chlorox with 70% ethanol as a disinfection medium and the contamination rate was also lower. The primary inoculation of Rhizobium was effected by cutting and smashed nodules at 30-40 days after sowing of lentil plant. Gram staining, biological microscope and scanned electron microscope observations showed that the bacteria were gram negative, rod shaped, the length to width ratio is 3:1 ‍and even smooth edges. Rhizobium was also positive in all the biochemical tests. In vitro culture initiation, microscopic reviews and biochemical testing for specific microbial isolates are essential for microbial fertilizer production.

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Isolation, identification and characterization of Rhizobium from lentil root nodule

Collee JG, Miles RS, Watt B. 1996. Test for the identification of bacteria. In: Collee JG, Faser AG, Marmion BP, Simmons A (editors). Mackie and McCartney. Practical Medical Microbiology 14th ed.

London: Churchill Livingstone 131-145.

Date RA, Halliday J. 1987.Collection, isolation, cultivation and maintenance of rhizobia. In Elkan, G.H., Ed. Symbiotic Nitrogen Fixation Technology.  Marcel Dekker, New York, NY p 1-27.

Datta A, Singh RK Kumar S. 2015. Isolation, characterization and growth of Rhizobium strains under optimum conditions for effective biofertilizer production. International Journal of Pharmaceutical Sciences Review and Research 32(1), 199-208.

Gauri SAK, Bhatt RP, Pant S, Bedi MK, Naglot A. 2011. Characterization of Rhizobium isolated from root nodules of Trifolium alexandrinum. Journal of Agricultural Technology 7(6), 1705-1723.

Javed K, Asghari B. 2008. Potential allelopathic effects of sunflowers on microorganisms. African Journal of Biotechnology 7(22), 4208-4211.

http://dx.doi.org/10.5897/AJB08.837

Wikipedia.  https://en.wikipedia.org/wiki/Rhizobium

Sawada H, Kuykendall LD, Young JM. 2003. Changing concepts in the systematics of bacterial nitrogen-fixing legume symbionts. Journal of General and Applied Microbiology 49(3), 155–79.

http://dx.doi.org/10.2323/jgam.49.155

Shruti S, Verinder W. 2019. Influence of different sterilizing methods on isolation endophytic bacteria from Rauvolfia serpentine. The Pharma Innovation Journal 8(1), 38-41.

Singh NP, Sewak S. 2013. Global perspective of chickpea research. AICRP on Chickpea 8-13.

Somasegaran P, Hoben HJ. 1994. Handbook for rhizobia: methods in Legume-Rhizobium technology. Springer-Verlag New York, Inc p 79-80.

Vincent JM. 1970. A manual for the practical study

of root nodule bacteria. IBP Hand Book No. 15. Blackwell, Scientific Publications, Oxford.

Wagner SC. 2011. Biological Nitrogen Fixation. Nature Education Knowledge 3(10), 15.

Wani PA, Khan MS. 2013. Isolation of multiple metal and antibiotic resistant Mesorhizobium sp. and their plant growth promoting activity. Research Journal of Microbiology 8, 25-35.

http://dx.doi.org/10.3923/jm.2013.25.35.

Most. Farhana Begom, Md. Giush Uddin Ahmed, Rebeka Sultana, Md. Zahurul Islam.
Isolation, identification and characterization of Rhizobium from lentil root nodule.
Int. J. Biosci. 18(4), 22-28, April 2021.
https://innspub.net/ijb/isolation-identification-and-characterization-of-rhizobium-from-lentil-root-nodule/
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