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The mcr-1 gene transformationthrough conjugation assay in avian pathogenic E. coli from Pakistan

By: Muhammad Rafique, Tariq Ahmed, Naeem Ali, Amir Afzal khan, Muzamil Shah, Atif Haroon, Muhammad Athar Abbas, Abdul Rahim, Naila Siddique

Key Words: E. coli, Conjugation assay, Resistance gene, PCR, mcr-1.

Int. J. Biosci. 15(3), 100-109, September 2019.

DOI: http://dx.doi.org/10.12692/ijb/15.3.100-109

Certification: ijb 2019 0120 [Generate Certificate]

Abstract

Escherichia coli are the normal flora of both human and animal intestinal tract. But still it is one of the most frequent members of the Enterobacteriaceae linked with extra intestinal infections. Similarly, global propagation of multidrug resistant (MDR) E. coli in food chains is a threat not only to animal health but also to human health. Colistin as the last resort antimicrobial in the arsenal of antibiotics. But in recent past, plasmid mediated colistin resistant gene has been detected in humans, livestock and on other retail meat products. The poultry industry is an important component of Pakistan gross domestic product. So, the aim of the study is to access the potential ability of mcr-1 resistant gene to transfer to other bacteria through conjugation. Biochemical and morphological identification was followed molecular identification. Therefore, DNA was extracted by DNA kit. The isolated mcr-1 bearing strains E. coli 79 was used to transformed mcr-1 gene to the donor E. coli J53, via conjugation. E. coli 79 isolate was used as donors, and E. coli J53 (resistant to sodium-azide) was used as the recipient strain. In result, the mcr-1 gene transformation was confirmed phenotypically and through PCR. In conclusion, the presence of mcr-1 gene in poultry isolated strains and their transformation potential was mad  e the last resort colistin on stack. Therefore, strict policies need to impose on the over use of the antimicrobial in animals.

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The mcr-1 gene transformationthrough conjugation assay in avian pathogenic E. coli from Pakistan

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Muhammad Rafique, Tariq Ahmed, Naeem Ali, Amir Afzal khan, Muzamil Shah, Atif Haroon, Muhammad Athar Abbas, Abdul Rahim, Naila Siddique.
The mcr-1 gene transformationthrough conjugation assay in avian pathogenic E. coli from Pakistan.
Int. J. Biosci. 15(3), 100-109, September 2019.
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