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Molecular identification of Taenia hydatigena isolated from domestic dogs in Baghdad city

By: Azhar Ali Faraj, Amer Murhum Al-Amery

Key Words: Taenia hydatigena, (18S rRNA )gene, phylogenetic tree, dogs, Iraq.

Int. J. Biosci. 13(4), 71-77, October 2018.

DOI: http://dx.doi.org/10.12692/ijb/13.4.71-77

Certification: ijb 2018 0010 [Generate Certificate]

Abstract

The aim of this present study is to detect the 18S rRNA gene sequences of T. hydatigena and to compare their genetic relatedness utilizing phylogenetic analysis. Twenty of T.  hydatigena adult worms were obtained from ten  naturally infected  domestic dogs after  treatment with albendazol , at a dose of  0.3% g/kg body weight . We obtained samples from dogs that treated in a small animal private clinics in Baghdad, Iraq, during the period from January to May2018. The results of phylogenetic analysis showed that proximity and the genetic dimension among the Taenia hydatigena isolate isolated from of dogs among themselves and the world are with more than 99% compatibility values. The molecular tools, including the sequence data of (18S rRNA) gene. It was the first study on Taenia hydatigena genotypes in  domestic dogs in Iraq.

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Molecular identification of Taenia hydatigena isolated from domestic dogs in Baghdad city

Dalimi A, Sattari A, Motamedi GH. 2006. A studyon intestinal helminthes of dogs, foxes and jackals inthe western part of Iran. Veterinary Parasitology 142, 129 – 133.

Lavikainen A, Haukisalmi V, Lehtinen MJ, Henttonen H, Oksanen A, Meri S. 2008. Aphylogeny of members of the family Taeniidae based on the mitochondrial cox1 and nad1 gene data. Parasitology 135, 1457 – 1467.

Murat K. 2005. Investigation of antigenic specificity Against Cysticercus tenuicollis cyst fluid antigen in dogs experimentally infected with Taenia hydatigena. T urkishJournal of Veterinary Animal Sciences 29, 835 – 840.

Deplazes P, Eckert J. 1988.Investigation of Taenia hydatigena infections in dogs Schweiz Arch Tierheilkd 130(6), 289-306.

Radfar MH, Tajalli   S, Jalalzadeh   M. 2005. Prevalence and  morphological characterization of Cysticercus tenuicollis (Taenia hydatigena cysticerci) from sheep and goats in Iran. Veterinarski arhiv 75, 469–476.

.

Gasser RB, Zhu X, McManus DP. 1999. NADH dehydrogenase subunit 1 and cytochrome c oxidase subunit I sequences compared for members of the genus Taenia (Cestoda). International Journal for Parasitology 29, 1965–1970.

González LM1, Villalobos N, Montero E, Morales J, Sanz RA, Muro A, Harrison LJ, Parkhouse RM, Gárate T. 2006. Differential molecular identification of Taeniid spp. and Sarcocystis spp. cysts isolated from infected pigs and cattle. Veterinary Parasitology 142(1-2), 95–101.

McManus DP. 2006. Molecular discrimination of  taeniid cestodes. Parasitology International 55, S31–

37.

Lavikainen A, Haukisalmi V, Lehtinen MJ, Henttonen H, Oksanen A, Meri S. 2008. Aphylogeny of members of the family Taeniidae based on the mitochondrial cox1 and nad1 gene data. Parasitology 135, 1457 – 1467.

Kassai T. 1999. Veterinary Helminthology, Butterworth-Heineman Publishing, Oxford, UK., p 222.

Bowman DD. 2009.Georgis’ Parasitology for Veterinarians.9th Ed. Westline industrial drive St. Louis, Missouri, p 142.

Rostami SSalavati RBeech RNBabaei ZSharbatkhori MBaneshi MRHajialilo EShad HHarandi MF. 2015. Molecular and  morphological characterization of the tapeworm Taenia hydatigena (Pallas, 1766) in sheep from Iran. Journal of Helminthology 89, 150–157.

Omar MA, Elmajdoub LO, Al-Aboody MS, Elsify AM, Elkhtam AO, Hussien AA. 2016. Molecular characterization of Cysticercus tenuicollis of slaughtered livestock in Upper Egypt governorates. Asian Pacific Journal Tropical Biomedicine 6(8), 706–708, 2016.

Jia WZ, Yan HB, Guo AJ, Zhu XQ, Wang YC, Shi WG. 2010. Complete mitochondrial genomes of Taenia multiceps, T. hydatigena and T. pisiformis additional molecular markers for a tapeworm genus of human and animal health significance. BMC Genomics 11(1), 447.

Chen ZH, Bao HE, Wu XJ, Yang TX, Hu XZ. 2010. Identification of Taeniasolium with abnormal number of scolex booklets. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 28(2), 108-12

Dai RSLiu GHSong HQLin RQYuan ZGLi MWHuang SYLiu WZhu XQ. 2012. Sequence variability in two mitochondrial DNA regions and internal transcribed spacer among three cestodes infecting animals and humans from China. Journal of Helminthology 86(2), 245-51.

Azhar Ali Faraj, Amer Murhum Al-Amery.
Molecular identification of Taenia hydatigena isolated from domestic dogs in Baghdad city.
Int. J. Biosci. 13(4), 71-77, October 2018.
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