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Mycochemicals and bioactivities of Aspergillus niger and Rhizomucor pusillus associated with vermicast

By: Jamile B. Sabado, Eden S. David, Mary Jhane G. Valentino

Key Words: Mycochemicals, Anti-oxidant, Phenolics, Anti-bacterial.

Int. J. Biosci. 13(3), 306-313, September 2018.

DOI: http://dx.doi.org/10.12692/ijb/13.3.306-313

Certification: ijb 2018 0010 [Generate Certificate]

Abstract

This study aimed to determine the mycochemical constituents and biological activities of fungi associated with vermicast namely: Aspergillus niger, Rhizomucor pusillus. Mycochemical screening of A. niger and R. pusillus ethanolic extract and fungal spent revealed the presence of flavonoids and terpenoids. Other bioactive compounds like tannins and alkaloids were only detected in A. niger and R. pusillus ethanolic extracts, respectively. For the antibacterial activity as protectant, at 12 hours and 24 hours of incubation, the least zone of colonization of Staphylococcys aureus and Escherichia coli were recorded in plates treated with A. niger ethanolic extract and R. pusillus ethanolic extracts. Whereas, zone of inhibition at 12 and 24 hours of incubation against the bacterial pathogens were recorded in ethanolic extract of A. niger and R. pusillus. For the DPPH radical scavenging activity, among the four extracts, A. niger ethanolic extract recorded the highest ability to scavenge free radicals. Thus, the aforementioned fungal organisms possess antimicrobial and anti-oxidant properties which can be attributed to their mycochemical components.

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Mycochemicals and bioactivities of Aspergillus niger and Rhizomucor pusillus associated with vermicast

Abbasi SA, Ramasamy EV. 2001. Biotechnological Methods of Pollution Control. Orient Longman, Universities Press India Ltd., Hyderabad, India, 168.

Blackwell M. 2011. The Fungi: 1, 2, 3… 5.1 million species. American Journal of Botany 98(3), 426-38. http://dx.doi.org/10.3732/ajb.1000298

Domínguez J. 2004. State of the art and new perspectives on vermicomposting research. In: C.A. Edwards (Ed.). Earthworm Ecology 2nd Edition, 401-424. CRC Press, Boca Raton, FL.

Duarte K, Teresa AP, Santos R, Freitas AC, Duarte AC. 2012. Analytical techniques for discovery of bioactive compounds from marine fungi. Trends in Analytical Chemistry 34, 97-110.

Durairaj B, Muthu S, Xavier T. 2015. Antimicrobial activity of Aspegillus niger synthesized titanium dioxide nanoparticles. Advances in Applied Science Research 6(1), 45-48.

Gajalakshmi S, Ramasamy EV, Abbasi SA. 2001. Potential of two epigeic and two anecic earthworm species in vermicomposting water hyacinth. Bio resource Technology 76, 177-181.

Gulcin I. 2007. Comparison of in-vitro antioxidant and antiradical activities of L-tyrosine and L-Dopa. Amino Acids 32(3), 431-438.

http://dx.doi.org/10.1007/s00726-006-0379-x

Halliwell B. 1995. How to characterize an antioxidant: An Update. Biochemical Society Symposia 61, 73-101.

Hajdu Z, Hohmann J, Forgo P, Martinek T, Dervaries M, Zupko I. 2007. Diterpenoids and flavonoids from the fruits of Vitex agnucastus and antioxidant activity of the fruit extracts and their constituents. Phytotherapy Research 4, 391-394.

Hameed A, Hussain SA, Yang J, Ijaz MU, Liu Q, Suleria HAR, Song Y. 2017. Antioxidants Potential of the Filamentous Fungi (Mucor circcinelloides, Colin Ratledge Center for Microbial Lipids. Journal of Agriculture Engineering and Food Science 1-20.

Hawksworth DL, Kirk PM, Sutton BC, Pegler DN. 1996. Ainsworth Bisby’s Dictionary of the Fungi. Revista do Instituto de Medicina Tropical de Sao Paulo 38(4), 272.

https://doi.org/10.1590/S003646651996000400018

IshikawaYK, Morimoto T, Sada Fujiwara T. 1995. Aurantionone produced by Penicillium aurantio-virens and its antioxidant activity, Journal of the American Oil Chemistry Society 41, 7-10.

Kalyani P, Hemalatha KPJ. 2017. In vitro Antimicrobial Potential of Aspergillus niger (MTCC-961). International Journal of Chem Tech Research 10(4), 430-435.

Kolak K, Ozturk M, Ozgokce F, Ulebelen A. 2006. Norditerpene alkaloids from Delphinium linearilobum. Phytochemistry 67, 2170-2175.

Koleckar V, Kubikova K, Rehakova Z, Kuca K, Jun D, Jahodar L, Opletal L. 2008. Condensed and hydrolysable tannins as antioxidants influencing the health. Mini-reviews in Medicinal Chemistry 8, 436-447.

Laboratory Manual for The Unesco. 1986. Sponsored workshop on the phytochemical, microbiological, and pharmacological screening of medicinal plants. Department of Chemistry, U.P. Diliman.

Ladoh YCF, Nyegue MA, Ngene JP, Benelesse GE, Lenta B, Wansi JD, Mpondo ME, Dibong SJ. 2015. Identification and Phytochemical Screening of Endophytic Fungi from Stems of Phragmanthera capitate (Sprengel) S. Balle (Loranthaceae). Journal of Applied Bioscience 90, 8355-8360.

http://dx.doi.org/10.4314/jab.v90i1.7

Lavelle P, Martin A. 1992. Small-scale and large-scale effects of endogeic earthworms on soil organic matter dynamics in soils of the humid tropics. Soil Biology and Biochemistry Journal 12, 1491–1498.

Madhusudan T, Mishra JK. 2017. Antibacterial activity of crude extract from Aspergillus niger isolated from the stilt roots of Rhizophora apiculate along South Andaman coast, India. Journal of Pharmacognosy and Phytochemistry 6(5), 1635-1638.

Maiquez JA, Pineda FG, Valentino MJG. 2016. Auxin like activity of Fungi associated with Bamboo in Rice (Oryza sativa L.) International Journal of Biology, Pharmacy and allied Sciences 5(7), 1707-1716.

Mishra A, Kumar S, Pandey AK. 2013. “Scientific validation of the medicinal efficacy of Tinospora cordifolia.” The Scientific World Journal 29, 29-34.

Nitya K, Murthy K, Pushpalatha KC, Joshi CG. 2011. Antioxidant activity and phytochemical analysis of endophytic fingi isolated from Lobelia nicotianfolia. Journal of Chemical and Pharmaceutical Research 3(5), 218-225.

Yadav M, Yadav A, Yadav JP. 2014. In vitro antioxidant activity and total phenolic content of endophytic fungi isolated from Eugenia jambolana Lam. Asia Pacific Journal of Tropical Medicine 7(1), 256-261.

https://doi.org/10.4103/1995-7645.240080

Yao LH, Jiang YM, Shi J, Jomas-Barberan FA, Datta N, Singanusong R, Caen SS. 2004. Flavonoids in food and their health benefits. Plant Foods for Human Nutrition 59(3), 113-122.

Yen GC, Lee CA. 1996. Antioxidant Activity of Extracts from Molds. Journal of Food Protection 59(12), 1327-1330.

https://doi.org/10.4315/0362-028X.81.1.4

Jamile B. Sabado, Eden S. David, Mary Jhane G. Valentino.
Mycochemicals and bioactivities of Aspergillus niger and Rhizomucor pusillus associated with vermicast.
Int. J. Biosci. 13(3), 306-313, September 2018.
https://innspub.net/ijb/mycochemicals-bioactivities-aspergillus-niger-rhizomucor-pusillus-associated-vermicast/
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