Nutraceutical potential and biological activities of selected medicinal plants
By: Sanam Zarif Satti, Sumera Zarif Satti, Shafqat Ali Khan, Wahiba Iqbal, Zahid Rauf, Hameed Ur Rehman, Nisar Ahmad, Syed Izhar Hussain Shah, Fazle Akbar, Habibullah, Shahid Raza
Key Words: Medicinal plants, proximate composition, Mineral profile, Phytochemical constituents, Antioxidant, Antimicrobial activity.
Int. J. Biosci. 15(2), 305-319, August 2019.
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In this study five wild medicinal plants viz. Caltha alba, Matricaria chamomilla, Ocimum basilicum, Phyllanthus emblica and Valeriana wallichii were screened to examine their proximate composition, mineral profile, phytochemical constituents, antioxidant and antimicrobial activity. The results of proximate analysis showed that promising moisture (10.20%), fat (3.63%) and protein (11.50%) was recorded in P. emblica while ash (8.87%) and fiber (14.90%) was noted in M. chamomilla and C. alba. However, least amount of moisture (6.92%), fiber (6.10%) and protein (7.42%) was examined in V. wallichii whereas ash (5.07%) and fat (1.56%) was found in C. alba and M. chamomilla. Among all the plant species maximum NFE was observed for V. wallichii (70.19) followed by M. chamomilla (60.23%) while minimum value was reported in P. emblica (57.99%). The results of mineral profile showed that all the selected plants possess notable amount of C, O, Mg, Si, P, K and Al however, S and Cl was not detected in V. wallichii while Ca was not found in O. basilicum. Phytochemical screening revealed that among all the plant species promising total phenolics (95.25 mg GAE g-1), total flavonoids (61.33 mg QE g-1) and antioxidant activity (84.70%) was recorded in P. emblica, V. wallichii and M. chamomilla whereas lowest values was investigated in O. basilicum (10.25 mg GAE g-1), C. alba (8.0 mg QE g-1) and V. wallichii (65.40%). The antimicrobial potential of crude methanol extract of the examined plant species was tested against opportunistic bacterial and fungal strains by using well diffusion assay. Among all the plant species M. chamomilla exhibits promising inhibitory potential against S. aureus (74.07%) followed by A. tumfaciens (69.23%), C. michiganensis (59.25%) and B. subtilis (53.57%). Likewise, V. wallichi have considerable activity against E. coli (56.0%) and X. compestris (46.15%). In case of fungicidal activity notable inhibitory zone was recorded for V. wallichii and P. emblica against Pacelomyces spp., (77.27% and 57.0%) followed by V. wallichii against A. niger (68.42%), however the selected plant also exhibited moderate to good and no activity against the tested fungal strains. It was concluded from the present results that antioxidant and antimicrobial potential of the plant extracts might be due to the presence of total phenols and total flavonoids content.
Nutraceutical potential and biological activities of selected medicinal plants
Abdul-Latif FM, Mohammad N, Edou Ali AA, Djama SO, Obam L, Bassole IHN, Dicko MH. 2011. Antimicrobial and antioxidant activities of essential oil and methanol extract of Matricaria chamomilla L. from Djibouti. Journal of Medicinal Plant Research 5, 1512-1517.
Abiy Y, Solmon D, Jacob DM, Christine CB, Matthias H, Martin GP. 2005. Antimicrobial flavonoids from the stem bark of Erythrina burtii. Fitoterapia. 96, 496-499.
Abolaji OA, Adebayo AH, Odesanmi OS. 2007. Nutritional qualities of three medicinal plant parts (Xylopia aethiopica, Blighia sapida and Parinari polyandra) commonly used by pregnant women in the Western part of Nigeria. Pakistan Journal of Nutrition 6, 665-668.
Adam AA, Omer AFA. 2015. Antibacterial activity of Ocimum basilicum (Rehan) leaf extract against bacterial pathogens in Sudan. American Journal of Research Communication 3, 94-99.
Agostoni C, Riva R, Giovannini M. 1995. Dietary fiber in waning foods of young children. Pediatrics 96, 1000-1005.
Aiyelaagbe OO, Osamudiamen PM. 2009. Phytochemical screening for active compounds in Mangifera indica. Plant Science Research 2, 11-13.
Akpabio UD, Ikpe EE. 2013. Proximate composition and nutrient analysis of Aneilema aequinoctiale leaves. Asian Journal Plant Science Research 3, 55-61.
Akpanyung EO, Udoh AP, Akpan EJ. 1995. Chemical composition of the edible leaves of Pterocarpus mildbreadii. Plant Foods Hum Nutrition 48, 209-2015.
Ali A. 2010. A comparative study of nutrients and mineral molar ratios of some plant foods with recommended dietary allowances. Journal of Food Science Technology 2, 104-108.
AOAC. 2012. Association of Official Analytical Chemists. Official Methods of Analysis 19th Edition (George Latimer.ed.). AOAC Intl, Arlington, Virginia, USA.
Ayo RG. 2013. Proximate and mineral compositions of the leaves and stem bark of Cassia nigricans Vahl. International Journal of Medicinal Plants Research 2, 242-246.
Balandrin MF, Kjocke AJ, Wurtele E. 1985. Natural plant chemicals: sources of industrial and mechanical materials. Science 228, 1154-1160.
Beaglehole R, Irwin A, Prentice T. 2004. The World health report 2004, changing history. Statistical Annex. Deaths by cause, sex and mortality stratum in WHO regions, estimates for 2002. WHO, Geneva, p 120-122.
Bello MO, Falade OS, Adewusi SRA, Olawore NO. 2008. Studies on the chemical compositions and antinutrients of some lesser known Nigeria fruits. African Journal of Biotechnology 7, 3972-3979.
Bibi S, Dastagir G, Hussain F, Sanaullah P. 2006. Elemental composition of Viola odorata Linn. Pakistan Journal of Plant Science 12, 141-143.
Blackwell WH. 1990. Poisonous and Medicinal Plants. Prentice Hall: Englewood Clifts, NJ.
Cheeke PR. 1998. Natural toxicants in Feeds, Forages, and Poisonous Plants, 2nd Edition. Interstate Pubs II.
Choudhary RK, Saroha AE, Swarnkar PL. 2011. Radical scavenging activity of phenolics and flavonoids in some medicinal plants of Indian Journal of Pharmacy Research. 4, 712-713.
Chung KT, Weichang I, Johnson MJK. 1998. Are tannins a double edged sword in biology and health? Trends Food Science Technology 4, 168-175.
Dastagir G, Pervez N. 2004. Elemental composition of Alstonia scholaris Linn. Pakistan Journal of Plant Science 10, 47-50.
Davey KR. 1989. A predictive model for combined
temperature and water activity on microbial growth during the growth phase. Journal of Applied Microbiology 65, 483-488.
Desai BB, Salunkhe DK. 1991. Fruits and vegetables. In: Salunkhe DK, Desh-pande SS (Eds). Foods of Plant Origin. Production, Technology and Human Nutrition, AVI, New York, p 301-355.
Dingman SL. 2002. Water in soils: infiltration and redistribution. Physical hydrology, 2nd edition, upper saddle river, New Jersey: Prentice-Hall, Inc. p 646.
Ebrahimzadeh MA, Nabavi SF, Nabavi SM, Pourmorad F. 2010. Nitric oxide radical scavenging potential of some Elburz medicinal plants. African Journal of Biotechnology 9, 5212-5217.
Ebun-Oluwa PO, Alade AS. 2007. Nutritional potential of berlandier nettle spurge (Jatropha cathatica) seed. Pakistan Journal of Nutrition 6, 345-348.
Effiong GS, Ibia TO, Udofia US. 2009. Nutritive and energy values of some wild fruit spices in Southeastern Nigeria. Journal of Environmental Agriculture Food Chemistry 8, 917-923.
Ene-obong HN. 1992. Nutritional evaluation, composition pattern and processing of underutilized traditional foods with particular reference to the African yam beans (Sphenostylis stenocarpa). PhD thesis, Department of Home Science and Nutrition, University of Nigeria, Nsukka.
Exarchou V, Nenadis U, Tsimidou U, Gerothanassis IP, Troganis A, Boskou D. 2002. Antioxidant activities and phenolic composition of extracts from Greek oregano, Greek sage and summer savory. Journal of Agriculture Food Chemistry 50,5294-5299.
Frazier WS, Westoff DC. 1978. Food Microbiology. 3rd Edition, McGraw Hill, New York, p 278-298.
Gill NS, Sharma R, Arora R, Bali M. 2012. Antioxidant and antibacterial activity of Hippophae rhamonides methanolic leaf extracts from dry temperate Agro-climate region of Himachal Pradesh. Journal of Plant Science 7, 194-200.
Gogoi P, Kalita JC. 2014. Proximate analysis and mineral components of some edible medicinally important leafy vegetables of Kamrup district of Assam, India. International Journal of Pharmacy Biological Science 5, 451-457.
Gul H, Khattak RA, Muhammad D. 2006. Yield and chemical composition of tobacco leaves of different cultivars as effected by four levels of potassium chloride. Pakistan Journal of Science and Industrial Research 49, 125-133.
Gupta R, Gupta GD. 2016. Pharmacognostic, phytochemical and antioxidant activity studies on Cordia obliqua willd. Leaf. International Journal of Pharm Science Research 7, 4669-4677.
Gupta SM, Gupta AK, Ahmed Z, Kumar A. 2011. Antibacterial and Antifungal Activity in Leaf, Seed Extract and Seed Oil of Seabuckthorn (Hippophae salicifolia D. Don) Plant. Journal of Plant Pathology and Microbiology 2, 1-4.
Hanif R, Iqbal Z, Iqbal M, Hanif S, Rasheed M. 2006. Use of vegetables as nutritional food: Role in human health. Journal of Agriculture Biological Science 1, 18-20.
Hassan LG, Ngaski MMA. 2007. Nutritional evaluation of Cassia siamea leaves. Journal of Chemical Society 32,137-143.
Hausladen A, Stamler JS. 1999. Nitrosative stress. Method Enzymology 300, 389-395.
Hoffman PC, Comb DK, Casler MD. 1998. Performance of lactating dairy cows fed alfalfa silage or perennial ryegrass silage. Journal Dairy Science 81, 162-168.
Ibanga OI, Okon DE. 2009. Minerals and anti-nutrients in two varieties of African pear (Dacryodes edulis). Journal of Food Technology 7, 106-110.
Jimoh FO, Oladiji AT. 2005. Preliminary Studies on Piliostigma thonningii seeds: Proximate analysis, mineral composition and phytochemical screening. African Journal of Biotechnology 4, 1439-1442.
Jones MM, Johnson DO, Netlerville JT, Wood JI, Joesten MD. 1985. Chemistry and Society. 5th Edition Saunders College Publishers USA, p 521- 577.
Kalim MD, Bhattacharyya D, Banerjee A, Chattopadhyay S. 2010. Oxidative DNA damage preventive activity and antioxidant potential of plants used in Unani system of medicine. BMC Complementary Alternative Medicine 10, 1-11.
Krishnaraju AV, Rao TV, Sundararaju D. 2005. Assessment of bioactivity of Indian medicinal plants using Brine shrimp (Artemisia salina) lethality assay. International Journal of Applied Science Engineering 2, 125-134.
Lajide L, Oseke MO, Olaoye OO. 2008. Vitamin C, fibre, lignin and mineral contents of some edible legume seedlings. Journal of Food Technology 6, 237-241.
Latif A, AhmadH, Begum S, Adnan M, Hussian S, Waseem S. 2003. Medicinal and other economic plants as substitute to forest logging in Miandam and Sulatanr valleys, Swat. Proceedings of international workshop on conservation and sustainable use of medicinal and aromatic plants in Pakistan. WWF-Pak. p 101-105.
Lavanya J, Selvam SP, Priya MJ, Jacintha P, Aradana M. 2016. Antioxidant and antimicrobial activity of selected medicinal plants against human oral pathogens. International Journal of Pharma Science 8, 71-78.
Lee, YM, Kim H, Hong JK, Kang BH, Kim SJ.
- Water extract of 1:1 mixture of Phellodendron cortex and Aralia cortex has inhibitory effects on oxidative stress in kidney of diabetic rats. Journal of Ethno pharmacology 73, 429-436.
Linder MC. 1991. Nutrition and metabolism of the major minerals, in: Linder MC (Ed.) Nutrition biochemistry and metabolism with clinical applications, 2nd Edition. Appleton and Lange, USA.
Liu X, Zhao M, Wang J, Yang B, Jiang Y. 2008. Antioxidant activity of methanolic extract of emblica fruit (Phyllanthus emblica L.) from six regions in China. Journal of Food Computational analysis 21, 219-228.
Mathew S, Abraham TE. 2006. Studies on the antioxidant activities of cinnamon (Cinnamomum verum) bark extracts, through various in vitro models. Food Chemistry 94, 520-528.
Mohanta B, Chakraborty A, Sudarshan M, Dutta RK, Baruah M. 2003. Elemental profile in some common medicinal plants of India. Its correlation with traditional therapeutic usage. Journal of Radio analytical Nuclear Chemistry 258, 175-179.
Mubashir S, Dar MY, Lone BA, Zargar MI, Shah WA. 2014. Anthelmintic, antimicrobial, antioxidant and cytotoxic activity of Caltha palustris var. alba Kashmir, India. Chinese Journal of Natural Medicine 12, 567-572.
Naidu PVL, Kumar KK, Kumar KM, Gunesh G, Narasimha MR. 2006. Antimicrobial activity of Achyranthes aspera. Biosciences, Biotechnology Research Asia 03, 1.
Nnamani CV, Oselebe HO, Agbatutu A. 2009. Assessment of nutritional values of three underutilized indigenous leafy vegetables of Ebonyi State, Nigeria. African Journal Biotechnology 8, 2321-2324.
Oktyabrsky O, Vysochina G, Muzyka G, Samoilova Z, Kukushkina T, Smirnova G. 2009. Assessment of anti-oxidant activity of plant extracts using microbial test systems. Journal of Applied Microbiology 106, 1175-1183.
Onwuka GI. 2005. Food Analysis and Instrumentation; Theory and Practice. Naphthalic prints, Surulere, Lagos, Nigeria, p 219- 230.
Ooi D, Iqbal S, Ismail M. 2012. Proximate composition, nutritional attributes and mineral composition of Peperomia pellucida L. (Ketumpangan Air) grown in Malaysia. Molecules. 17, 11139-11145.
Osman MA. 2004. Chemical and nutrient analysis of baobab (Adansonia digitata) fruit and seed protein solubility. Plant Foods Human Nutrition 59, 29-33.
Pamela CC, Richard AH, Denise RF. 2005. Lippincotts illustrated Reviews Biochemistry 3rd Edition, Lippincott Williams and Wilkins, Philadelphia, p 335- 388.
Pandey MAB, Abidi S, Singh RP. 2006. Nutritional evaluation of leafy vegetables. Paratha. Journal of Human Ecology 19, 155-156.
Parekh J, Chanda SV. 2007. In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turkish Journal of Biology 31, 53-58.
Park SK, Choi CW, Lee YK, Hyun PJ, Arokiyaraj S. 2014. Antimicrobial and free radical scavenging activity of Cordia obliqua W. (Boraginaceae) fruit extracts. Journal of Pure Applied Microbiology 8, 117-120.
Park, YS, S, Jung SG, Kang Heo BK, Arancibia-Avila P, Toledo F. 2008. Antioxidants and proteins in ethylene treated kiwifruits. Food Chemistry 107, 640-648.
Patil DD, Mhaske DK, Wadhawa GC. 2011. Antibacterial and antioxidant study of Ocimum basilicum Labiatae (sweet basil). Journal of Advance Pharmacy and Educational Research 2, 104-112.
Perez C, Pauli M, Bazevque P. 1990. An antibiotic assay by the agar well diffusion method. Acta Biology Medical Expression 15, 113-115.
Raghu HS, Ravindra P. 2010. Antimicrobial activity and phytochemical study of Phyllanthus emblica linn. International Journal of Pharmacy Studies Research 1, 30-33.
Rajurkar NS, Damame MM. 1997. Elemental analysis of some herbal plants used in the treatment of cardiovascular diseases by NAA and AAS. Journal of Radio analytical Nuclear Chemistry 219, 77-80.
Said S, Saeed HMA, DeSilva LA, Zubairy HN, Bano Z. 1996. Medicinal Herbal, A Textbook for Medical Students and Doctors. Published by Hamdard Foundation Pakistan Nazimabad, Karachi 74600, Pakistan 1, 272-291.
Saldanha LG. 1995. Fiber in the diet of U.S. Children: Results of national survey. Pediatrics 96, 994-996.
Scalbert A, Manach C, Remesy C, Morand C. 2005. Dietary polyphenols and the prevention of diseases. Critical Reviews in Food Science Nutrition 45, 287-306.
Scott WS. 1957. Water relations of food spoilage microorganisms. Advance Food Research 7, 84-127.
Shinwari ZK, Rehman M, Watanabe T, Yoshikawa T. 2006. A Pictorial guide to medicinal plants of Pakistan. Kohat University of Science and Technology Pakistan.
Singleton VL, Rossi JA. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology Viticulture 16, 144-158.
Soare LC, Ferdes M, Stefanov S, Denkova Z, Nicolova R, Denev P, Bejan C, Paunescu A. 2012. Antioxidant activity, polyphenols content and antimicrobial activity of several native pteridophytes of Romania Liliana Cristina. Not. Botanical Horticulture Agrobiology 40, 53-57.
Sofowora A. 1982. Medicinal plants and traditional medicine in Africa. Wiley, Nigeria, p 33-34.
Sudhanshu Rao N, Mittal S, Menghani E. 2012. Evaluation of antioxidant properties of Valeriana wallichi to scavenge free radicals. Asian Journal of Pharmacology Clinical Research 5, 238-240.
Tairo TV, Jesse TN, Lukhoba WC, Herbert VML. 2011. Nutritive and antinutritive qualities of mostly preferred edible woody plants in selected dry lands of Iringa District, Tanzania. Pakistan Journal of Nutrition 10, 786-791.
Thomas T. 2011. Preliminary antibacterial and phytochemical evaluation of Dryopteris cochleata (D. Don) Global Pharm Technology 3, 4-8.
Sulieman MM, Dzenda T, Sani CA. 2008. Antidiarreal activity of the methanol stem-bark extract of Annona senagalensis. Journal of Ethnopharmacology 116, 125-130.
Taesotikul T, Panthong A, Kanjanapothi D, Verpoorti R, Scheffer JJC. 2003. Anti-inflammatory, antipyretic and antinoceceptive activities of Tabernaemontena pandacaque poir. Journal of Ethnopharmacology 84, 31-35.
Tehrian AA, Babaei M, Vafaei AA, Jarrahi M, Jadidi M, Sadeghai H. 2009. Antinoceceptive effect of Hydrochloric extracts of Thymus vulgaris. Pak Journal of Pharmacological science 22(1), 83-89.
Tesema S, Makonnen E. 2015. In vivo analgesic and antipyretic activities of N-Butanol and water reactions of Ocimum suave aqueous leaves extracts in mice. Ethiop Journal of health and science 25(2), 139-146.
Treas GE, Evans WC. 1985. Textbook of Pharmacognosy. Published by Balleire Tindal, London.
Udgire MS, Pathade GR. 2013. Evaluation of antimicrobial activities and phytochemical constituents of extracts of Valeriana wallichii. Asian Journal of Plant Science Research 3, 55-59.
Vaya J, Belinky GA, Aviram M. 1997. Antioxidant constituents from Licorice roots: isolation, structure elucidation and anti-oxidative capacity toward LDL oxidation. Free Radical Bio Medical 23, 302-313.
Williamson G, Dupont MS, Heaney RK, Roger G, Rhodes MJ. 1997. Induction of slutathione S transferase activity in hepG2 cells by extracts of fruits and vegetables. Food Chemistry 2, 157-160.
Zhang X. 1976. Traditional medicine and WHO, World health, the magazine of World Health Organization 49th year 2, 4-5.
Nutraceutical potential and biological activities of selected medicinal plants.
Int. J. Biosci. 15(2), 305-319, August 2019.
By Authors and International Network for
Natural Sciences (INNSPUB)