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Nutrients utilization and biomass production by microalgae culture development in wastewater

By: Faryal Kabir, Muhammad Gulfraz, Ghazala Kaukab Raja, Muhammad Inam-ul-Haq, Muhammad Sheeraz Ahmad, Muhammad Farooq Nasir, Muhammad Awais, Iram Batool

Key Words: Microalgae, Treatment, Biomass, Bioproducts

Int. J. Biosci. 12(6), 460-469, June 2018.

DOI: http://dx.doi.org/10.12692/ijb/12.6.460-469

Certification: ijb 2018 0104 [Generate Certificate]

Abstract

The growth of microalgae for production of biofuels is beneficial when sewage wastewater is used as a growth media because of two reasons; wastewater treatment and cost effective biomass generation as compared to artificial growth medium cultivation. In this study, three microalgae species (Chlorella vulgaris, Selenastrum sp. and Chlorococcum humicola) were isolated from freshwater as well as wastewater sample and were purified. Treatment of sewage was carried out for effective utilization of nutrients by these strains, whereas biomass and lipid productivity was measured accordingly. The wastewater treatment by C. vulgaris shows 98% COD and more than 90% TN removal efficiency. Total phosphorus and ammonia was removed almost 100% by all of the strains. The treatment efficiency and biomass productivity of Selenastrum sp. was less as compared to other strains while C. vulgaris shows higher lipid productivity. According to results the biomass productivity can be further enhanced by addition of external nitrogen and carbon source to wastewater media. While treatment of domestic wastewater along with production of biomass is a key strategy to produce many biobased co-products including biodiesel.

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Nutrients utilization and biomass production by microalgae culture development in wastewater

Anonymous. 2000. National Environmental Quality Standards (NEQS). The Gazette of Pakistan. Ministry of Environment, Local Government and Rural Development, Government of Pakistan.

AOAC (Association of Official Analytical Chemists). 2000. Official methods of analysis of AOAC International. 17th Ed. Gaithersburg, MD, Washington, USA.

Aslan S, Kapdan IK. 2006. Batch kinetics of nitrogen and phosphorus removal from synthetic wastewater by algae. Ecological Engineering 28, 64-70.

Benemann JR, Oswald WJ. 1996. Systems and economic analysis of microalgae pond for conversion of CO2 to biomass. Final report, United States Department of Energy.

Berges JA, Charlebois DO, Mauzerall DC, Falkowski PG. 1996. Differential effects of nitrogen limitation on photosynthetic efficiency of photosystems I and II in microalgae. Plant Physiology 110, 689-696.

Bhatnagar A, Bhatnagar M, Chinnasamy S, Das K. 2010. Chlorella minutissima – promising fuel alga for cultivation in municipal wastewaters. Applied Biochemistry and Biotechnology 161, 523-536.

Chinnasamy S, Rao PH, Bhaskar S, Rengasamy R, Singh M. 2012. Algae: a novel biomass feedstock for biofuels. In: Arora R, Ed. Microbial Biotechnology: Energy and Environment 224-239.

Crites R, Technobanoglous G. 1998. Small and decentralized wastewater management systems, McGraw-Hill. New York. USA.

Darley WM. 1982. Algal biology: A physiological approach. Basic microbiology 9, 168. Oxford: Blackwell scientific publications.

Deng XY, Gao K, Zhang RC, Addy M, Lu Q, Ren HY, Chen P, Liu YH, Ruan R. 2017. Growing Chlorella vulgaris on thermophilic anaerobic digestion swine manure for nutrient removal and biomass production. Bioresource Technology 243, 417-425.

Dere S, Gunes T, Sivaci R. 1998. Spectrop hotometric determination of chlorophyll-a, b and total carotenoid contents of some algae species using different solvents. Turkish Journal of Botany 22, 13-17.

Dincer K. 2008. Lower emissions from biodiesel combustion. Energy Sources: Part A 30, 963-8.

Droop M. 1955. Carotenogenesis in Haematococcus pluvialis. Nature 175, 42.

Feng P, Deng Z, Hua Z, Fanc L. 2011. Lipid accumulation and growth of Chlorella zofingiensis in flat plate photobioreactors outdoors. Bioresource Technology 102, 10577-10584.

Feng Y, Li C, Zhang D. 2011. Lipid production of Chlorella vulgaris cultured in artificial wastewater medium. Bioresource Technology 102, 101-105.

Godos I, Blanco S, Garcia-Encina PA, Becares E, Munoz R. 2009. Long-term operation of high rate algal ponds for the bioremediation of piggery wastewaters at high loading rates. Bioresource Technology 100, 4332-4339.

Gonzalez C, Marciniak J, Villaverde S, Leon C, Garcia-Encina PA, Munoz R. 2008b. Efficient nutrient removal from swine manure in a tubular biofilm photo-bioreactor using algae bacteria consortia. Water Science and Technology 58, 95-102.

Guldhe A, Misra R, Singh P, Rawat I, Bux F. 2015. An innovative electrochemical process to alleviate the challenges for harvesting of small size microalgae by using non-sacrificial carbon electrodes. Algal Research 19, 292-298.

Gutzeit G, Lorch D, Weber A, Engels M, Neis U. 2005. Bioflocculent algal-bacterial biomass improves low-cost wastewater treatment. Water Science and Technology 52, 9-18.

Hach 2008. DR 5000 Spectrophotometer Procedures Manual. Edition 2. Hach Company, Colorado, USA.

Hempel N, Petrick I, Behrendt F. 2012. Biomass productivity and productivity of fatty acids and amino acids of microalgae strains as key characteristics of suitability for biodiesel production. Journal of Applied Phycology 24, 1407-1418.

Hu B, Min M, Zhou W, Du Z, Mohr M, Chen P, Zhu J, Cheng Y, Liu Y, Ruan R. 2012. Enhanced mixotrophic growth of microalga Chlorella sp. on pretreated swine manure for simultaneous biofuel feedstock production and nutrient removal. Bioresource Technology 126, 71-79.

Ji F, Liu Y, Hao R, Li G, Zhou Y, Dong R. 2014. Biomass production and nutrients removal by a new microalgae strain Desmodesmus sp. in anaerobic digestion wastewater. Bioresource Technology 161, 200-207.

Khan M, Yoshida N. 2008. Effect of L-glutamic acid on the growth and ammonium removal from ammonium solution and natural wastewater by Chlorella vulgaris NTM06. Bioresource Technology 99, 575-82.

Khoshmanesh A, Lawson F, Prince IG. 1996. Cadmium uptake by unicellular green microalgae. The Chemical Engineering Journal and the Biochemical Engineering Journal 62, 81-88.

Kobayashi M, Kakizono T, Yamaguchi K, Nishio N, Nagai S. 1992. Growth and astaxanthin formation of Haematococcus pluvialis in heterotrophic and mixotrophic conditions. Journal of Fermentation and Bioengineering 74, 17-20.

Kshirsagar AD. 2013. Bioremediation of wastewater by using microalgae: An experimental study. International Journal of life sciences Biotechnology and Pharma Research 2, 339-346.

Laliberte G, Lessard P, Delanoue J, Sylvestre S. 1997. Effect of phosphorus addition on nutrient removal from wastewater with the cyanobacterium Phormidium bohneri. Bioresource Technology 59, 227-33.

Lau PS, Tam NFY, Wong YS. 1995. Effect of algal density on nutrient removal from primary settled wastewater. Environmental Pollution 89, 59-66.

Lee RE. 1999. Phycology. 3rd Ed. Cambridge University Press, Cambridge, UK, 50 pp.

Li X, Hu HY, Gan K, Sun YX. 2010. Effects of different nitrogen and phosphorus concentrations on the growth, nutrient uptake, and lipid accumulation of a freshwater microalga Scenedesmus sp. Bioresource Technology 101, 5494-500.

Ma X, Zheng H, Addy M, Anderson E, Liu Y, Chen P, Ruan R. 2016. Cultivation of Chlorella vulgaris in wastewater with waste glycerol: strategies for improving nutrients removal and enhancing lipid production. Bioresource Technology 207, 252-261.

Ma X, Zhou W, Fu Z, Cheng Y, Min M, Liu Y, Zhang Y, Chen P, Ruan R. 2014. Effect of wastewater-borne bacteria on algal growth and nutrients removal in wastewater-based algae cultivation system. Bioresource Technology 167, 8-13.

Maestrini SY, Robert JM, Leftley JW, Collos Y. 1986. Ammonium thresholds for simultaneous uptake of ammonium and nitrate by oyster-pond algae. Journal of Experimental Marine Biology and Ecology 102, 75-98.

Martin C, De la Noun J, Picard G. 1985. Intensive cultivation of freshwater microalgae on aerated pig manure. Biomass 7, 245-259.

Martinez ME, Sanchez S, Jimenez JM, Yousfi EL, Munoz L. 2000. Nitrogen and phosphorus removal from urban wastewater by the microalga Scenedesmus obliquus. Bioresource Technology 73, 263-272.

Miao X, Wu Q. 2006. Biodiesel production from heterotrophic microalgal oil. Bioresource Technology 97, 841-846.

Milledge JJ. 2011. Commercial application of microalgae other than as biofuels: a brief review. Reviews in Environmental Science and Biotechnology 10, 31-41.

Nurdogan Y, Oswald WJ. 1995. Enhanced nutrient removal in high-rate ponds. Water Science and Technology 31, 33-43.

Oswald WJ, Gotaas HB, Golueke CG, Kellen WR, Gloyna EF, Hermann ER. 1957. Algae in waste treatment. Sewage and Industrial Wastes 29, 437-457.

Park J, Jin HF, Lim BR, Park KY, Lee K. 2010. Ammonia removal from anaerobic digestion effluent of livestock waste using green alga Scenedesmus sp. Bioresource Technology 101, 8649-8657.

Perez MVJ, Castillo PS, Romera O, Moreno DF, Martínez CP. 2004. Growth and nutrient removal in free and immobilized planktonic green algae isolated from pig manure. Enzyme and Microbial Technology 34, 392-398.

Prescott GW. 1962. Algae of the Western great lakes area. WMC Brown Company publishers, Dubuque, Iowa. 997.

Ramsundar P, Guldhe A, Singh P, Bux F. 2017. Assessment of municipal wastewaters at various stages of treatment process as potential growth media for Chlorella sorokiniana under different modes of cultivation. Bioresource Technology 227, 82-92.

Reynolds CS. 1984. The ecology of freshwater phytoplankton. Cambridge: Cambridge University Press 157-191.

Ruiz-Marin A, Mendoza-Espinosa LG, Stephenson T. 2010. Growth and nutrient removal in free and immobilized green algae in batch and semi-continuous cultures treating real wastewater. Bioresource Technology 101, 58-64.

Shi J, Podola B, Melkonian M. 2007. Removal of nitrogen and phosphorus from wastewater using microalgae immobilized on twin layers: an experimental study. Journal of Applied Phycology 19, 417-423.

Sires I, Brillas E. 2012. Remediation of water pollution caused by pharmaceutical residues based on electrochemical separation and degradation technologies: a review. Environment International 40, 212-229.

Wang L, Li Y, Chen P, Min M, Chen Y, Zhu J, Ruan R. 2010. Anaerobic digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp. Bioresource Technology   101, 2623-2628.

Wang L, Min M, Li Y, Chen P, Chen Y, Liu Y, Wang Y, Ruan R. 2010. Cultivaton of green algae Chlorella sp. in different wastewaters from municipal wastewater treatment plant. Applied Biochemistry and Biotechnology 162, 1174-1186.

Zhou W, Li Y, Min M, Hu B, Chen P, Ruan R. 2011. Local bioprospecting for high-lipid producing microalgal strains to be grown on concentrated municipal wastewater for biofuel production. Bioresource Technology 102, 6909-6919.

Zhou W, Min M, Li Y, Hu B, Ma X, Cheng Y, Liu Y, Chen P, Ruan R. 2012. A hetero-photoautotrophic two-stage cultivation process to improve wastewater nutrient removal and enhance algal lipid accumulation. Bioresource Technology 110, 448-455.

Zhu LD. 2015. Biorefinery as a promising approach to promote microalgae industry: an innovative framework. Renewable and Sustainable Energy Reviews 41, 1376-1384.

Faryal Kabir, Muhammad Gulfraz, Ghazala Kaukab Raja, Muhammad Inam-ul-Haq, Muhammad Sheeraz Ahmad, Muhammad Farooq Nasir, Muhammad Awais, Iram Batool.
Nutrients utilization and biomass production by microalgae culture development in wastewater.
Int. J. Biosci. 12(6), 460-469, June 2018.
https://innspub.net/ijb/nutrients-utilization-biomass-production-microalgae-culture-development-wastewater/
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